Increased oxidative DNA damage, inducible nitric oxide synthase, nuclear factor κB expression and enhanced antiapoptosis-related proteins in Helicobacter pylori-infected non-cardiac gastric adenocarcinoma

Figure 1 Concentrations of 8-OHdG. A: Higher concentration of 8-OhdG in cancer tissues compared with adjacent non- cancer tissues. B: Higher concentration of 8-OhdG in cancer tissues of patients with and without H pylori infection. C: Significantly higher F8-OHdG concentrations in stage 3 and 4 patients ^aP < 0.05, N: non-cancer tissue, C: cancer tissue, Hp(-): without H pylori infection, Hp(+): with H pylori infection, 1&2: gastric cancer stages 1 and 2, 3&4: gastric cancer stages 3 and 4.

Figure 2 Immunoblot analysis of factors affecting cell death. A: Immunoblot analysis of iNOS. 1: Significantly higher concentra-tions of iNOS in cancer tissues compared with adjacent non-cancer tissues. 2: Significantly higher concentrations of iNOS in cancer tissues of patients with and without H pylori infection. 3: Significantly higher iNOS concentrations in stage 3 and 4 patients. #=H pylori infected, ^aP < 0.05, ^bP < 0.01, N: non-cancer tissue, C: cancer tissue, Hp(-): without H pylori infection, Hp(+): with H pylori infection, 1&2: gastric cancer stages 1 and 2, 3&4: gastric cancer stages 3 and 4. B: Immunoblot analysis of NFκB. 1: Significantly higher concentrations of NFκB in cancer tissues in comparison with adjacent non-cancer tissues. 2: Significantly higher concentrations of NFκB in cancer tissues of patients with and without H pylori infection. 3: Significantly higher NFκB concentrations in stage 3 and 4 patients #=H pylori infected, P < 0.05, N: non-cancer tissue, C: cancer tissue, Hp( -): without H pylori infection, Hp(+): with H pylori infection, 1&2: gastric cancer stages 1 and 2, 3&4: gastric cancer stages 3 and 4. C: Immunoblot analysis of MEKK1. 1: Concentra-tions of MEKK1 in cancer tissues and adjacent non-cancer tissues. 2: Significantly higher concentrations of MEKK1 in cancer tissues of patients without H pylori infection. 3. No differences in MEKK1 concentrations in cancer tissues with different stages. #= H pylori infected, ^aP < 0.05, N: non-cancer tissue, C: cancer tissue, Hp(-): without H pylori infection, Hp(+): with H pylori infection, 1&2: gastric cancer stages 1 and 2, 3&4: gastric cancer stages 3 and 4. D: Immunoblot analysis of Caspase 3. 1: Similar concentrations of Caspase 3 in cancer tissues and adjacent non-cancer tissues. 2: Similar concentrations of Caspase 3 in cancer tissues of patients with and without H pylori infection. 3: Significantly lower Caspase 3 concentrations in stage 1 and 2 patients. #=H pylori infected, ^bP < 0.01, N: non- cancer tissue, C: cancer tissue, Hp(-): without H pylori infection, Hp(+): with H pylori infection, 1&2: gastric cancer stages 1 and 2, 3&4: gastric cancer stages 3 and 4. E: Immunoblot analysis of Bcl-2. 1: Similar concentrations of Bcl-2 in cancer tissues and adjacent non-caner tissues. 2: No differences in concentrations of Bcl-2 in cancer tissues of patients with and without H pylori infection. 3: Significantly higher Bcl-2 concentrations in stage 1 and 2 patients. #= H pylori infected, ^bP < 0.01, N: non-cancer tissue, C: cancer tissue, Hp( -): without H pylori infection, Hp(+): with H pylori infection, 1&2: gastric cancer stages 1 and 2, 3&4: gastric cancer stages 3 and 4. F: Immunoblot analysis of Mcl-1. 1: Significantly higher concentrations of Mcl-1 in cancer tissues in comparison with adjacent non-cancer tissues. 2: Significantly higher concentration of Mcl-1 in cancer tissues of patients with and without H pylori infection. 3: Significant higher Mcl -1 concentrations in stage 3 and 4 patients than #= H pylori infected, ^aP < 0.05, N: non -cancer tissue, C: cancer tissue, Hp(-): without H pylori infection, Hp(+): with H pylori infection, 1&2: gastric cancer stages 1 and 2, 3&4: gastric cancer stages 3 and 4. G: Immunoblot analysis of IAP. 1: Significantly higher concentrations of IAP in cancer tissues compared with adjacent non- cancer tissues, 2: Significantly higher concentrations of IAP in cancer tissues with H pylori infection, 3: Significantly higher IAP concentrations in stage 3 and 4 patients #=H pylori infected, ^aP < 0.05, N: non -cancer tissue, C: cancer tissue, Hp(-): without H pylori infection, Hp(+): with H pylori infection, 1&2: gastric cancer stages 1 and 2, 3&4: gastric cancer stages 3 and 4.