A rapid and efficient method to express target genes in mammalian cells by baculovirus

Figure 1 Plasmids pFB-EGFP, pFB-CMV-EGFPA and pFB-CMV-EGFPB.

Figure 2 Fluorescence photos of Sf9 cells infected with recombinant baculoviruses. A: BacV–EGFP; B: BacV-CMV-EGFPA; C: BacV-CMV-EGFPB.

Figure 3 Fluorescence photos (up) and FCM analysis (down) of HepG2 cells transduced with recombinant baculoviruses. A: BacV–EGFP; B: BacV-CMV-EGFPA; C: BacV-CMV-EGFPB.

Figure 4 Effect of different moi and incubation time on gene transfer and expression efficiencies in HepG2 cells transduced with BacV-CMV-EGFPA.

Figure 5 Reporter gene transfer and expression efficiencies at different times in HepG2 cells transduced with BacV-CMV-EGFPA.

Figure 6 Comparison of gene-transfer and expression efficiencies in different mammalian cell lines transduced with BacV-CMV-EGFPA.