Modulation of human enteric epithelial barrier and ion transport function by Peyer’s patch lymphocytes

Figure 1 Comparison of time-dependent changes in transepithelial resistance (TER) of different culture groups. Data in all panels are mean ± SE for 4 experiments, significant differences relative to its own 4-d of culture in Caco-2 alone (^bP < 0.01), and to its own 6^th day culture of Caco-2 alone (^dP < 0.001) are indicated.

Figure 2 Effect of Shigella F2a-12 LPS on transepithelial resis-tance (TER) of different culture groups. Cultured monolayers were exposed to LPS 48-h prior to TER measurement on the 6 d of culture. Comparison of TER in the absence or presence of Shigella F2a-12 LPS among the three culture groups. The values indicate mean ± SE; n = 5; ^aP < 0.05 (compared to its own control).

Figure 3 Effect of Shigella F2a-12 LPS on the Forskolin-induced Isc. Comparison of the Forskolin (4 mg/mL, basoateral addition) -induced Isc in the absence or presence of LPS for 8-h and 24-h under different culture conditions. The values indicate mean ± SE; n = 12; ^aP < 0.05 (compared to its own control) ; ^cP < 0.05 and ^bP < 0.001 (compared to its own LPS treatment for 8-h).

Figure 4 Induction of hIL-8 expression in Caco-2 cells by Shi-gella F2a-12 LPS in different culture groups. Comparison of hIL-8 expression in Caco-2 cells in the absence or presence of LPS for 8-h and 24-h between three culture groups by semi-quantitative RT-PCR with Peyer’s patch lymphocytes as nega-tive control. The values indicate mean ± SE; ^aP < 0.05, ^bP < 0.01 (compared to its own control) ; ^cP < 0.05 (compared to separated co-culture LPS pre-treatment for 8-h).

Figure 5 Comparison of Shigella F2a-12 LPS-induced hIL-8 release from different culture groups by ELISA. Culture groups and Peyer’s patch lymphocytes alone (control, 1 × 10⁶ cells) were treated with LPS for 8-h and 24-h. The presented values indicate mean ± SE; n = 8; ^bP < 0.001 (compared to its own control) ; ^aP < 0.05 and ^dP < 0.001 (compared to its own LPS treatment for 8-h).

Figure 6 Induction of mIL-6 expression by Shigella F2a-12 LPS in different culture groups. Comparison of mIL-6 expression in the absence or presence of LPS for 8-h and 24-h among the culture groups by semi-quantitative RT-PCR with mouse Peyer’s patch lymphocytes as positive control. The values indicate mean ± SE ^aP < 0.01 (compared to the mixed control).

Figure 7 Comparison of Shigella F2a-12 LPS-induced mIL-6 release from different culture groups by ELISA. Culture groups and Peyer’s patch lymphocytes (1 × 10⁶ cells) were treated with LPS for 8-h and 24-h. The values indicate mean ± SE; n = 5; ^bP < 0.01 (compared to mixed co-culture control) and ^dP < 0.01 (compared to the mixed LPS treatment for 8-h).