In vitro cultivation of human fetal pancreatic ductal stem cells and their differentiation into insulin-producing cells

Figure 1 After being cultured for 24 h, a few cells were adherented on the bottom of non-sticky flasks, some of the cells processed protrusions (× 100).

Figure 2 After being cultured for 2 wk in the media added the keratinocyte growth factor, the number of cells increased, the cells became fusiform with a few processes (× 100).

Figure 3 Most cells of culture were positive for CK-19 immu-noreactivity after being cultured for 2 wk (× 100).

Figure 4 The cells were cobblestone pattern after being cul-tured for 4 wk (× 150).

Figure 5 The cells were cobblestone pattern and formed mono-layer after being cultured for 6 wk (× 100).

Figure 6 The cells were induced to form pancreatic islet-like struc-tures after being cultured in the media containing 17. 8 mmol/L glucose for 1 wk (× 100).

Figure 7 After being cultured in the medium containing 17. 8 mmol/L glucose for 1 wk, many cells in islet-like struc-tures were positve for insulin immunoreactivity (× 150).

Figure 8 After being cultured in the medium containing 17 mmol/L glucose for 1 wk, a few cells in islet-like structures were posi-tive for glucagon immunoreactivity (× 150).

Figure 9 Percentage of immunopositive cells induced by glucose.