Gastric Cancer
Copyright ©The Author(s) 2004.
World J Gastroenterol. Jan 1, 2004; 10(1): 26-30
Published online Jan 1, 2004. doi: 10.3748/wjg.v10.i1.26
Figure 1
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Figure 1 Diagram of the construction of the expression vector PL(TT)SN.
Figure 2
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Figure 2 Segment analyses of pEGFP-TI and pEGFP-TT by restriction endonuclease digestion. Lane 1: DNA marker (from top to bottom: 1 543, 994, 697, 515 and 337 bp); Lane 2: pEGFP-TI/EcoR I and Hpa I (TK gene 1 128 bp); Lane 3: pEGFP-TI/ EcoRI and XhoI (TK + IRES 1.71kb); Lane 4: pEGFP-TI/XhoI and BamH I (IL-2 gene 456 bp); Lane 5: pEGFP-TT/EcoRI and HpaI (TK gene 1 128 bp); Lane 6: pEGFP-TT/EcoRI and XhoI (TK + IRES 1.71 kb); Lane 7: pEGFP-TT/XhoI and BamHI (TNF-α gene 474 bp).
Figure 3
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Figure 3 Transient expression of the recombinant genes in SGC7901 cells. A: Transient expression of pEGFP-TT in SGC7901 cells × 100. B: Transient expression of pEGFP-TI in SGC7901 cells × 100.
Figure 4
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Figure 4 Stably expressed recombinant protein in transduced SGC7901 cells confirmed by Western blot. Lane 1: IL-2 (15ku) expressed in SGC/TI cells; Lane 2: TNF-α (17ku) expressed in SGC/TT cells.