Basic Research
Copyright ©2008 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. Feb 28, 2008; 14(8): 1204-1211
Published online Feb 28, 2008. doi: 10.3748/wjg.14.1204
Establishment and characterization of a new cell line derived from human colorectal laterally spreading tumor
Xin-Ying Wang, Zhou-Sheng Lai, Chung-Man Yeung, Ji-De Wang, Wen Deng, Hoi Yee Li, Yu-Jing Han, Hsiang-Fu Kung, Bo Jiang, Marie Chia-mi Lin
Xin-Ying Wang, Zhou-Sheng Lai, Ji-De Wang, Yu-Jing Han, Bo Jiang, Department of Gastroenterology, Nanfang Hospital, Nanfang Medical University, Tong He, Guangzhou 510520, Guangdong Province, China
Chung-Man Yeung, Hoi Yee Li, Marie Chia-mi Lin, Department of Chemistry, the University of Hong Kong, Pokfulam Road, Hong Kong, China
Wen Deng, Department of Anatomy, the University of Hong Kong, Pokfulam Road, Hong Kong, China
Hsiang-Fu Kung, Li Ka Shing Institute of Health Sciences, The Centre for Emerging Infectious Diseases, the Chinese University of Hong Kong, Hong Kong, China
Correspondence to: Bo Jiang, MD, Department of Gastroenterology, Nanfang Hospital, Nanfang Medical University, Tong He, Guangzhou 510520, Guangdong Province, China. drjiang@163.com
Telephone: +86-20-61641558
Fax: +86-20-61641558
Received: October 18, 2007
Revised: December 12, 2007
Published online: February 28, 2008
Abstract

AIM: To study the molecular mechanism of laterally spreading tumor (LST), a cell line [Laterally Spreading Tumor-Rectum 1 (LST-R1)] was derived and the characteristics of this cell line were investigated.

METHODS: A new cell line (LST-R1) originated from laterally spreading tumor was established. Properties of the cell line were characterized using scanning and transmission electron microscopy, immunohistochemistry method, cytogenetic analysis and nude mice xenograft experiments. In vitro invasion assay, cDNA microarray and Western blotting were used to compare the difference between the LST-R1 and other colorectal cancer cell lines derived from prudent colon cancer.

RESULTS: Our study demonstrated that both epithelial special antigen (ESA) and cytokeratin-20 (CK20) were expressed in LST-R1. The cells presented microvilli and tight junction with large nuclei. The karyotypic analysis showed hyperdiploid features with structural chromosome aberrations. The in vivo tumorigenicity was also demonstrated in nude mice xenograft experiments. The invasion assay suggested this cell line has a higher invasive ability. cDNA microarray and Western blotting show the loss of the expression of E-cadherin in LST-R1 cells.

CONCLUSION: We established and characterized a colorectal cancer cell line, LST-R1 and LST-R1 has an obvious malignant tendency, which maybe partially attributed to the changes of the expression of some adhesion molecules, such as E-cadherin. It is also a versatile tool for exploring the original and progressive mechanisms of laterally spreading tumor and the early colon cancer genesis.

Keywords: LST-R1; Cell line; Invasion; E-cadherin