Donlon NE, Davern M, O’Connell F, Sheppard A, Heeran A, Bhardwaj A, Butler C, Narayanasamy R, Donohoe C, Phelan JJ, Lynam-Lennon N, Dunne MR, Maher S, O’Sullivan J, Reynolds JV, Lysaght J. Impact of radiotherapy on the immune landscape in oesophageal adenocarcinoma. World J Gastroenterol 2022; 28(21): 2302-2319 [PMID: 35800186 DOI: 10.3748/wjg.v28.i21.2302]
Reader's ID:
05116814
Submitted on:
June 27, 2022, 07:41
Reader Expertise:
Reader’s expertise on the topic of the manuscript
Conflicts-of-Interest Statement:
Does the reader have a conflict of interest?
Reader Comment Standards for Published Articles:
1 Title
Does the title reflect the main subject/hypothesis of the manuscript?
2 Abstract
Does the abstract summarize and reflect the work described in the manuscript?
3 Key Words
Do the key words reflect the focus of the manuscript?
4 Background
Does the manuscript adequately describe the background, present status and significance of the study?
5 Methods
Does the manuscript describe methods (e.g., experiments, data analysis, surveys, and clinical trials, etc.) in adequate detail?
6 Results
Are the research objectives achieved by the experiments used in this study?
Has the study made meaningful contributions towards research progress in this field?
7 Discussion
Does the manuscript interpret the findings adequately and appropriately, highlighting the key points concisely, clearly and logically?
Are the findings and their applicability/relevance to the literature stated in a clear and definite manner?
Is the Discussion accurate and does it discuss the paper’s scientific significance and/or relevance to clinical practice sufficiently?
8 Illustrations and Tables
Are the figures, diagrams and tables sufficient, good quality and appropriately illustrative of the paper contents?
Do figures require labeling with arrows, asterisks, etc., or better legends?
9 Biostatistics
Does the manuscript meet the requirements of biostatistics?
10 Units
Does the manuscript meet the requirements of use of SI units?
11 References
Does the manuscript appropriately cite the latest, important and authoritative references in the Introduction and Discussion sections?
Does the author self-cite, omit, incorrectly cite and/or over-cite references?
12 Quality of manuscript organization and presentation
Is the manuscript concisely and coherently organized and presented?
Are the style, language and grammar accurate and appropriate?
13 Ethics statements
For all manuscripts involving human studies and/or animal experiments, author(s) must submit the related formal ethics documents that were reviewed and approved by their local ethical review committee. Did the manuscript meet the requirements of ethics?
Scientific Quality:
The overall quality of the manuscript, based on the above-listed criteria, should be evaluated and classified according to the following five categories
Language Quality:
Language quality (style, grammar, and spelling) should be evaluated and classified according to the following five categories.
Reader Comments:
In order to describe the immunophenotype of immune checkpoint (IC) expression in OAC patients before and after radiotherapy, evaluate the effect of checkpoint blocking on cell viability, and pay more and more attention to the spread of anti-tumor immune response, this article studies the effect of radiation and checkpoint blocking on cell viability in OAC patients. A mixed combination of in vitro and in vitro models was used. The serum immune proteins were quantified by enzyme-linked immunosorbent assay. Flow cytometry staining was used to evaluate IC expression in OAC cell lines and OAC biopsies in vitro. The cell viability was evaluated by cell counting kit-8 analysis under the radiation conditions with or without IC blocking. The experimental process and data results are clear. The results of this study have great implications for clinical practice. In the new adjuvant treatment environment, ICIS and ionizing radiation have synergistic effects, which also have obvious implications for the treatment of specific patients in the future. It is a rare good article
Reply from the Editorial Office:
Thank you very much for your comments.