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Graça VC, Ferreira ICFR, Santos PF. Bioactivity of the Geranium Genus: A Comprehensive Review. Curr Pharm Des 2020; 26:1838-1865. [PMID: 31942856 DOI: 10.2174/1381612826666200114110323] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/23/2019] [Accepted: 11/20/2019] [Indexed: 12/22/2022]
Abstract
BACKGROUND Plants from the Geranium genus, which comprises about 400 species, have been used since ancient times in the practice of traditional medicines throughout the world. Therefore, herbal preparations based on Geranium species have found wide usage for the treatment of a variety of ailments. The aim of this work is to present a review, as comprehensive as possible, of the studies concerning different biological activities of Geranium species. METHODS Relevant data were obtained through systematic computer searches from major reputed scientific databases, particularly Web of Science and Scopus. Occasionally, information issued in primary sources not covered by these databases was also included provided published as peer-reviewed literature. This review covers the literature disclosed till the end of 2018. RESULTS Accompanying the increasing interest in herbal medicines in general, the evaluation of the biological properties of medicinal plants from the Geranium genus has been addressed thoroughly, mostly over the last two decades. Geranium species are endowed with a number of different biological activities. Herein, we present a survey of the results of the studies concerning these different biological activities. CONCLUSION Most studies found in the literature effectively contribute to scientifically validate the beneficial properties of Geranium plants claimed by traditional medicines and medical herbalism and demonstrate that many of them possess evident therapeutic properties.
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Affiliation(s)
- Vânia C Graça
- Centro de Quimica-Vila Real (CQ-VR), Universidade de Tras-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal.,Centre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB) - Vila Real, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal.,Centro de Investigacao de Montanha (CIMO), Instituto Politecnico de Braganca, Campus de Santa Apolonia, 5300-253 Braganca, Portugal
| | - Isabel C F R Ferreira
- Centro de Investigacao de Montanha (CIMO), Instituto Politecnico de Braganca, Campus de Santa Apolonia, 5300-253 Braganca, Portugal
| | - Paulo F Santos
- Centro de Quimica-Vila Real (CQ-VR), Universidade de Tras-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal
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Urrutia-Hernández TA, Santos-López JA, Benedí J, Sánchez-Muniz FJ, Velázquez-González C, De la O-Arciniega M, Jaramillo-Morales OA, Bautista M. Antioxidant and Hepatoprotective Effects of Croton hypoleucus Extract in an Induced-Necrosis Model in Rats. Molecules 2019; 24:molecules24142533. [PMID: 31373296 PMCID: PMC6680924 DOI: 10.3390/molecules24142533] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/14/2019] [Revised: 07/05/2019] [Accepted: 07/09/2019] [Indexed: 12/21/2022] Open
Abstract
The aim of this study was to evaluate the antioxidant and hepatoprotective activity of Croton hypoleucus (EC). The present work reports the first pharmacological, toxicological, and antioxidant studies of EC extract on liver injury. Liver necrosis was induced by thioacetamide (TAA). Five groups were established: Croton Extract (EC), thioacetamide (TAA), Croton extract with thioacetamide (EC + TAA), vitamin E with thioacetamide (VE + TAA) and the positive control and vehicle (CT). For EC and EC + TAA, Wistar rats (n = 8) were intragastrically pre-administered for 4 days with EC (300 mg/kg.day) and on the last day, EC + TAA received a single dose of TAA (400 mg/kg). At 24 h after damage induction, animals were sacrificed. In vitro activity and gene expression of superoxide dismutase (SOD), catalase (Cat), and Nrf2 nuclear factor were measured. The results show that EC has medium antioxidant properties, with an IC50 of 0.63 mg/mL and a ferric-reducing power of 279.8 µM/mg. Additionally, EC reduced hepatic damage markers at 24 h after TAA intoxication; also, it increased SOD and Cat gene expression against TAA by controlling antioxidant defense levels. Our findings demonstrated the hepatoprotective effect of EC by reducing hepatic damage markers and controlling antioxidant defense levels. Further studies are necessary to identify the mechanism of this protection.
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Affiliation(s)
- Thania Alejandra Urrutia-Hernández
- Área Académica de Farmacia, Universidad Autónoma del Estado de Hidalgo, Mariano Abasolo 600, Colonia Centro, Pachuca, Hidalgo CP 42000, Mexico
| | - Jorge Arturo Santos-López
- Departamento de Farmacología, Farmacognosia y Botánica, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal S/N, 28040 Madrid, Espana
| | - Juana Benedí
- Departamento de Farmacología, Farmacognosia y Botánica, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal S/N, 28040 Madrid, Espana
| | - Francisco Jose Sánchez-Muniz
- Departamento de Nutrición y Ciencia de los Alimentos, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal S/N, 28040 Madrid, Espana
| | - Claudia Velázquez-González
- Área Académica de Farmacia, Universidad Autónoma del Estado de Hidalgo, Mariano Abasolo 600, Colonia Centro, Pachuca, Hidalgo CP 42000, Mexico
| | - Minarda De la O-Arciniega
- Área Académica de Farmacia, Universidad Autónoma del Estado de Hidalgo, Mariano Abasolo 600, Colonia Centro, Pachuca, Hidalgo CP 42000, Mexico
| | - Osmar Antonio Jaramillo-Morales
- Área Académica de Farmacia, Universidad Autónoma del Estado de Hidalgo, Mariano Abasolo 600, Colonia Centro, Pachuca, Hidalgo CP 42000, Mexico
| | - Mirandeli Bautista
- Área Académica de Farmacia, Universidad Autónoma del Estado de Hidalgo, Mariano Abasolo 600, Colonia Centro, Pachuca, Hidalgo CP 42000, Mexico
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Vargas-Mendoza N, Vázquez-Velasco M, González-Torres L, Benedí J, Sánchez-Muniz FJ, Morales-González JA, Jaramillo-Morales OA, Valadez-Vega C, Bautista M. Effect of Extract and Ellagic Acid from Geranium schiedeanum on the Antioxidant Defense System in An Induced-Necrosis Model. Antioxidants (Basel) 2018; 7:178. [PMID: 30513625 PMCID: PMC6316715 DOI: 10.3390/antiox7120178] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/09/2018] [Revised: 11/20/2018] [Accepted: 11/26/2018] [Indexed: 02/07/2023] Open
Abstract
Geranium schiedeanum has been used in traditional therapies as an antiseptic, antipyretic, and as analgesic. The present study was designed to evaluate the pretreatment with G. schiedeanum total extract (GS) and its active metabolites on stimulating the endogenous antioxidant defense system (EADS): catalase (Cat), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione reduction index (RI GSH/GSSG) in rat liver treated with a sublethal dose (6.6 mmol/Kg) of thioacetamide (TAA) in order to probe the capacity of GS and the active compounds to reduce liver injury. This was assessed by measuring aspartate aminotransferase (AST), alanine aminotransferase (ALT), and total bilirubin (BILT) in rats pretreated or not with TAA, and pretreated or not with GS and its metabolites. The results showed that GS was able to induce the production of EADS enzymes, increasing redox index GSH/GSSG at 24 and 48 h after intoxication, and both the extract and the ellagic acid exhibited a significant reduction of hepatic damage markers. Our data confirmed the hepatoprotective effect of GS and its metabolites, like ellagic acid, support the possible use of this extract in the treatment of liver injury.
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Affiliation(s)
- Nancy Vargas-Mendoza
- Área Académica de Farmacia, Universidad Autónoma del Estado de Hidalgo, Abasolo N. 600, Colonia Centro, Pachuca, Hidalgo CP 42000, Mexico.
| | - Miguel Vázquez-Velasco
- Facultad de Farmacia, Universidad Complutense de Madrid, Ciudad Universitaria, Plaza de Ramón y Cajal S/N, 28040 Madrid, Spain.
| | - Laura González-Torres
- Facultad de Farmacia, Universidad Complutense de Madrid, Ciudad Universitaria, Plaza de Ramón y Cajal S/N, 28040 Madrid, Spain.
| | - Juana Benedí
- Facultad de Farmacia, Universidad Complutense de Madrid, Ciudad Universitaria, Plaza de Ramón y Cajal S/N, 28040 Madrid, Spain.
| | - Francisco José Sánchez-Muniz
- Facultad de Farmacia, Universidad Complutense de Madrid, Ciudad Universitaria, Plaza de Ramón y Cajal S/N, 28040 Madrid, Spain.
| | - Jose Antonio Morales-González
- Instituto Politécnico Nacional, Escuela Superior de Medicina, Plan de San Luis y Díaz Mirón, Col. Casco de Santo Tomás, Del. Miguel Hidalgo, Hidalgo DF 11340, Mexico.
| | - Osmar Antonio Jaramillo-Morales
- Área Académica de Farmacia, Universidad Autónoma del Estado de Hidalgo, Abasolo N. 600, Colonia Centro, Pachuca, Hidalgo CP 42000, Mexico.
- Área Académica de Enfermería, Universidad Autónoma del Estado de Hidalgo, Abasolo N. 600, Colonia Centro, Pachuca, Hidalgo CP 42000, Mexico.
| | - Carmen Valadez-Vega
- Área Académica de Medicina, Universidad Autónoma del Estado de Hidalgo, Abasolo N. 600, Colonia Centro, Pachuca, Hidalgo CP 42000, Mexico.
| | - Mirandeli Bautista
- Área Académica de Farmacia, Universidad Autónoma del Estado de Hidalgo, Abasolo N. 600, Colonia Centro, Pachuca, Hidalgo CP 42000, Mexico.
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Chung APYS, Gurtu S, Chakravarthi S, Moorthy M, Palanisamy UD. Geraniin Protects High-Fat Diet-Induced Oxidative Stress in Sprague Dawley Rats. Front Nutr 2018; 5:17. [PMID: 29616223 PMCID: PMC5864930 DOI: 10.3389/fnut.2018.00017] [Citation(s) in RCA: 41] [Impact Index Per Article: 5.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/22/2017] [Accepted: 02/27/2018] [Indexed: 12/02/2022] Open
Abstract
Geraniin, a hydrolysable polyphenol derived from Nephelium lappaceum L. fruit rind, has been shown to possess significant antioxidant activity in vitro and recently been recognized for its therapeutic potential in metabolic syndrome. This study investigated its antioxidative strength and protective effects on organs in high-fat diet (HFD)-induced rodents. Rats were fed HFD for 6 weeks to induce obesity, followed by 10 and 50 mg/kg of geraniin supplementation for 4 weeks to assess its protective potential. The control groups were maintained on standard rat chows and HFD for the same period. At the 10th week, oxidative status was assessed and the pancreas, liver, heart and aorta, kidney, and brain of the Sprague Dawley rats were harvested and subjected to pathological studies. HFD rats demonstrated changes in redox balance; increased protein carbonyl content, decreased levels of superoxide dismutase, glutathione peroxidase, and glutathione reductase with a reduction in the non-enzymatic antioxidant mechanisms and total antioxidant capacity, indicating a higher oxidative stress (OS) index. In addition, HFD rats demonstrated significant diet-induced changes particularly in the pancreas. Four-week oral geraniin supplementation, restored the OS observed in the HFD rats. It was able to restore OS biomarkers, serum antioxidants, and the glutathione redox balance (reduced glutathione/oxidized glutathione ratio) to levels comparable with that of the control group, particularly at dosage of 50 mg geraniin. Geraniin was not toxic to the HFD rats but exhibited protection against glucotoxicity and lipotoxicity particularly in the pancreas of the obese rodents. It is suggested that geraniin has the pharmaceutical potential to be developed as a supplement to primary drugs in the treatment of obesity and its pathophysiological sequels.
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Affiliation(s)
- Alexis Panny Y S Chung
- School of Medicine and Health Sciences, Monash University Malaysia, Sunway City, Malaysia
| | - Sunil Gurtu
- School of Medicine and Health Sciences, Monash University Malaysia, Sunway City, Malaysia
| | | | - Mohanambal Moorthy
- School of Medicine and Health Sciences, Monash University Malaysia, Sunway City, Malaysia
| | - Uma D Palanisamy
- School of Medicine and Health Sciences, Monash University Malaysia, Sunway City, Malaysia
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Meng X, Li Y, Li S, Gan RY, Li HB. Natural Products for Prevention and Treatment of Chemical-Induced Liver Injuries. Compr Rev Food Sci Food Saf 2018; 17:472-495. [DOI: 10.1111/1541-4337.12335] [Citation(s) in RCA: 51] [Impact Index Per Article: 7.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/08/2017] [Revised: 12/26/2017] [Accepted: 12/29/2017] [Indexed: 12/19/2022]
Affiliation(s)
- Xiao Meng
- Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Dept. of Nutrition, School of Public Health; Sun Yat-sen Univ.; Guangzhou 510080 China
| | - Ya Li
- Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Dept. of Nutrition, School of Public Health; Sun Yat-sen Univ.; Guangzhou 510080 China
| | - Sha Li
- School of Chinese Medicine, Li Ka Shing Faculty of Medicine; The Univ. of Hong Kong; Hong Kong China
| | - Ren-You Gan
- Dept. of Food Science and Engineering, School of Agriculture and Biology; Shanghai Jiao Tong Univ.; Shanghai 200240 China
| | - Hua-Bin Li
- Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Dept. of Nutrition, School of Public Health; Sun Yat-sen Univ.; Guangzhou 510080 China
- South China Sea Bioresource Exploitation and Utilization Collaborative Innovation Center; Sun Yat-sen Univ.; Guangzhou 510006 China
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Moreno E, Gayosso JA, Montejano JR, Almaguer G, Vázquez N, Cruz C, Mercado A, Bobadilla NA, Gamba G, Sierra A, Ramírez V. Geraniin is a diuretic by inhibiting the Na +-K +-2Cl - cotransporter NKCC2. Am J Physiol Renal Physiol 2017; 314:F240-F250. [PMID: 29046296 DOI: 10.1152/ajprenal.00221.2017] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/15/2023] Open
Abstract
Geranium seemannii Peyr is a perennial plant endemic to central Mexico that has been widely used for its diuretic effect, but the responsible compound of this effect is unknown as well as the mechanism by which the diuretic effect is achieved. Geraniin is one of the compounds isolated from this kind of geranium. This study was designed to determinate whether geraniin possesses diuretic activity and to elucidate the mechanism of action. Geraniin was extracted and purified from Geranium seemannii Peyr. Male Wistar rats were divided into four groups: 1) Control, 2) 75 mg/kg of geraniin, 3) 20 mg/kg of furosemide, and 4) 10 mg/kg of hydrochlorothiazide. Each treatment was administered by gavage every 24 h for 7 days. The urinary excretion of electrolytes and the fractional excretion of sodium (FENa) were determined. To uncover the molecular target of geraniin, Xenopus laevis oocytes were microinjected with cRNAs encoding the Na+-Cl- cotransporter (NCC) and the Na+-K+-2Cl- cotransporter NKCC2 to functionally express these cotransporters. Geraniin significantly increased diuresis, natriuresis, and calciuresis to a similar extent as was observed in the furosemide-treated rats. Consistent with the furosemide-like effect, in X. laevis oocytes, geraniin significantly reduced the activity of NKCC2, with no effect on NCC activity. In contrast to furosemide, the effect of geraniin on NKCC2 was irreversible, apparently due to its inhibitory effect on heat shock protein 90. Our observations suggest that geraniin could have a potential role in the treatment of hypertension or edematous states.
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Affiliation(s)
- Erika Moreno
- Department of Nephrology and Mineral Metabolism, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Tlalpan, Mexico City, Mexico
| | - Juan A Gayosso
- Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo Pachuca, Hidalgo, Mexico
| | - José R Montejano
- Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo Pachuca, Hidalgo, Mexico
| | - Georgina Almaguer
- Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo Pachuca, Hidalgo, Mexico
| | - Norma Vázquez
- Molecular Physiology Unit, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico
| | - Cristino Cruz
- Department of Nephrology and Mineral Metabolism, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Tlalpan, Mexico City, Mexico
| | - Adriana Mercado
- Department of Nephrology, Instituto Nacional de Cardiología Ignacio Chávez, Tlalpan, Mexico City, Mexico
| | - Norma A Bobadilla
- Department of Nephrology and Mineral Metabolism, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Tlalpan, Mexico City, Mexico.,Molecular Physiology Unit, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico
| | - Gerardo Gamba
- Department of Nephrology and Mineral Metabolism, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Tlalpan, Mexico City, Mexico.,Molecular Physiology Unit, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico.,Tecnológico de Monterrey, Escuela de Medicina y de Ciencias de la Salud, Monterrey, Nuevo León , México
| | - Alfredo Sierra
- Escuela Superior de Medicina, Instituto Politécnico Nacional , Mexico City, Mexico
| | - Victoria Ramírez
- Department of Nephrology and Mineral Metabolism, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Tlalpan, Mexico City, Mexico
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Morales-González Á, Bautista M, Madrigal-Santillán E, Posadas-Mondragón A, Anguiano-Robledo L, Madrigal-Bujaidar E, Álvarez-González I, Fregoso-Aguilar T, Gayosso-Islas E, Sánchez-Moreno C, Morales-González JA. Nrf2 modulates cell proliferation and antioxidants defenses during liver regeneration induced by partial hepatectomy. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 2017; 10:7801-7811. [PMID: 31966628 DOI: pmid/31966628] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Subscribe] [Scholar Register] [Received: 04/21/2017] [Accepted: 05/21/2017] [Indexed: 02/07/2023]
Abstract
The objective was to determine the regulatory dynamic of Nrf2 during liver regeneration and the administration of EtOH and/or the G. schiedeanum extract. Male Wistar rats weighing 200-230 g were subjected to a 70% partial hepatectomy; they were then divided into three groups (groups 1-3). During the experiment, animals in Group 1 drank only water. The other two groups (2-3) received an intragastric dose of ethanol (1.5 g/kg BW, solution at 40% in isotonic saline solution). Additionally, rats in group 3 received a geranium extract daily at a dose of 300 mg/kg BW i.g. EtOh and/or Geranium schiedeanum was administered to rats with regenerating livers for 7 days. At the end of treatment, the activity was determined of the antioxidant enzymes, DNA concentration, TBARS, and TAC, in addition to the expression of Nrf-2, Cyclin D1, and Nqo1. EtOH increased ROS and Nrf-2, which activated the antioxidant defenses and delayed liver proliferation. On the other hand, Geranium schiedeanum exerted an antioxidant effect, diminishing ROS, but Nrf-2 expression increased, favoring liver proliferation through the increase of DNA concentration and the overexpression of Cyclin D1, however it did not activate the antioxidant defenses. In sum, it can be concluded that Nrf-2 possesses a regulatory dynamic that is evident in the presence of a toxic agent (EtOH) and/or a phytochemical agent with antioxidant capacity (Geranium schiedeanum) during liver regeneration.
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Affiliation(s)
- Ángel Morales-González
- Escuela Superior de Cómputo, Instituto Politécnico Nacional México
- Área Académica de Farmacia, ICSa, Universidad Autónoma del Estado de Hidalgo México
- Laboratorio de Medicina de Conservación, Escuela Superior de Medicina, Instituto Politécnico Nacional México
- Laboratorio de Farmacología Molecular, Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional México
- Laboratorio de Genética, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional México
- Depto. de Fisiología, Laboratorio de Hormonas y Conducta, ENCB campus Zacatenco, Instituto Politécnico Nacional México
- Área Académica de Enfermería, ICSa, Universidad Autónoma del Estado de Hidalgo México
| | - Mirandeli Bautista
- Área Académica de Farmacia, ICSa, Universidad Autónoma del Estado de Hidalgo México
| | - Eduardo Madrigal-Santillán
- Laboratorio de Medicina de Conservación, Escuela Superior de Medicina, Instituto Politécnico Nacional México
| | - Araceli Posadas-Mondragón
- Laboratorio de Medicina de Conservación, Escuela Superior de Medicina, Instituto Politécnico Nacional México
| | - Liliana Anguiano-Robledo
- Laboratorio de Farmacología Molecular, Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional México
| | - Eduardo Madrigal-Bujaidar
- Laboratorio de Genética, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional México
| | - Isela Álvarez-González
- Laboratorio de Genética, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional México
| | - Tomás Fregoso-Aguilar
- Depto. de Fisiología, Laboratorio de Hormonas y Conducta, ENCB campus Zacatenco, Instituto Politécnico Nacional México
| | - Evila Gayosso-Islas
- Área Académica de Enfermería, ICSa, Universidad Autónoma del Estado de Hidalgo México
| | | | - José A Morales-González
- Laboratorio de Medicina de Conservación, Escuela Superior de Medicina, Instituto Politécnico Nacional México
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Madrigal-Santillán E, Bautista M, Gayosso-De-Lucio JA, Reyes-Rosales Y, Posadas-Mondragón A, Morales-González &A, Soriano-Ursúa MA, García-Machorro J, Madrigal-Bujaidar E, Álvarez-González I, Morales-González JA. Hepatoprotective effect of Geranium schiedeanum against ethanol toxicity during liver regeneration. World J Gastroenterol 2015; 21:7718-7729. [PMID: 26167072 PMCID: PMC4491959 DOI: 10.3748/wjg.v21.i25.7718] [Citation(s) in RCA: 18] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 12/09/2014] [Revised: 02/25/2015] [Accepted: 04/09/2015] [Indexed: 02/06/2023] Open
Abstract
AIM To evaluate the effect of an extract of Geranium schiedeanum (Gs) as a hepatoprotective agent against ethanol (EtOH)-induced toxicity in rats. METHODS Male Wistar rats weighing 200-230 g were subjected to a 70% partial hepatectomy (PH); they were then divided into three groups (groups 1-3). During the experiment, animals in group 1 drank only water. The other two groups (2-3) drank an aqueous solution of EtOH (40%, v/v). Additionally, rats in group 3 received a Gs extract daily at a dose of 300 mg/kg body weight intragastically. Subsequently, to identify markers of liver damage in serum, alanine aminotransferase, aspartate aminotransferase, albumin and bilirubin were measured by colorimetric methods. Glucose, triglyceride and cholesterol concentrations were also determined. In addition, oxidative damage was estimated by measuring lipid peroxidation [using thiobarbituric-acid reactive substances (TBARS)] in both plasma and the liver and by measuring the total concentration of antioxidants in serum and the total antioxidant capacity in the liver. In addition, a liver mass gain assessment, total DNA analysis and a morpho-histological analysis of the liver from animals in all three groups were performed and compared. Finally, the number of deaths observed in the three groups was analyzed. RESULTS Administration of the Geranium shiedeanum extract significantly reduced the unfavorable effect of ethanol on liver regeneration (restitution liver mass: PH-EtOH group 60.68% vs PH-Gs-EtOH group 69.22%). This finding was congruent with the reduced levels of hepatic enzymes and the sustained or increased levels of albumin and decreased bilirubin in serum. The extract also modified the metabolic processes that regulate glucose and lipid levels, as observed from the serum measurements. Lower antioxidant levels and the liver damage induced by EtOH administration appeared to be mitigated by the extract, as observed from the TBARs (PH-EtOH group 200.14 mmol/mg vs PH-Gs-EtOH group 54.20 mmol/mg; P < 0.05), total status of antioxidants (PH-EtOH group 1.43 mmol/L vs PH-Gs-EtOH group 1.99 mmol/L; P < 0.05), total antioxidant capacity values, liver mass gain and total DNA determination (PH-EtOH group 4.80 mg/g vs PH-Gs-EtOH 9.10 mg/g; P < 0.05). Overall, these processes could be related to decreased mortality in these treated animals. CONCLUSION The administered extract showed a hepatoprotective effect, limiting the EtOH-induced hepatotoxic effects. This effect can be related to modulating oxido-reduction processes.
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