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Omadang L, Chamai M, Ejobi F, Erume J, Oba P, Ocaido M. Prevalence of cystic echinococcosis among livestock in pastoral and agro-pastoral areas in Uganda. Parasitology 2024; 151:68-76. [PMID: 38012850 PMCID: PMC10941043 DOI: 10.1017/s0031182023001154] [Citation(s) in RCA: 5] [Impact Index Per Article: 5.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/27/2023] [Revised: 11/16/2023] [Accepted: 11/20/2023] [Indexed: 11/29/2023]
Abstract
Cystic echinococcosis (CE) remains a significant challenge in Uganda with precise status largely undocumented in most communities. To determine CE prevalence, post-mortem examination was done on 14 937 livestock (5873 goats, 1377 sheep, 3726 zebu cattle and 3054 Ankole cattle) slaughtered in abattoirs in the districts of Moroto in Karamoja region, Kumi in Teso region and Nakasongola and Luwero in Buganda region. The overall CE prevalence was 21.9% in sheep, 15.2% in zebu cattle, 5.5% in goats and 2.1% in Ankole cattle. Moroto district had a higher prevalence of CE than other districts with 31.3% in zebu cattle, sheep 28%, goats 29.1% and (0%) in Ankole cattle. On organ locations, the lungs were the most affected in all livestock in all the study areas. Considering cyst fertility, 33.9, 1.7 and 6.4% of Ankole cattle, sheep and zebu cattle respectively had fertile cysts in the liver while 4.5% of goats and 4% Ankole cattle had fertile cysts in the lungs. In conclusion, CE is widespread and occurs among cattle, sheep and goats in pastoral and agro-pastoral areas in Uganda. Therefore, there is an urgent need to create awareness among the communities on role of livestock in CE epidemiology and transmission.
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Affiliation(s)
- Leonard Omadang
- College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda
- Faculty of Agriculture and Animal Sciences, Department of Animal Production and Management, Busitema University, Arapai Campus, Soroti, Uganda
| | - Martin Chamai
- College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda
| | - Francis Ejobi
- Directorate of Research and Innovations, Faculty of Health Sciences, Soroti University, Soroti, Uganda
| | - Joseph Erume
- College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda
| | - Peter Oba
- National Agricultural Research Organization (NARO), Abi Zonal Agricultural Research and Development Institute (Abi ZARDI), Arua, Uganda
| | - Michael Ocaido
- College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda
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Chen CS, Zhang YG, Wang HJ, Fan HN. Effect and mechanism of reactive oxygen species-mediated NOD-like receptor family pyrin domain-containing 3 inflammasome activation in hepatic alveolar echinococcosis. World J Gastroenterol 2023; 29:2153-2171. [PMID: 37122606 PMCID: PMC10130966 DOI: 10.3748/wjg.v29.i14.2153] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 12/17/2022] [Revised: 02/01/2023] [Accepted: 03/16/2023] [Indexed: 04/13/2023] Open
Abstract
BACKGROUND The NOD-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome is a significant component of the innate immune system that plays a vital role in the development of various parasitic diseases. However, its role in hepatic alveolar echinococcosis (HAE) remains unclear.
AIM To investigate the NLRP3 inflammasome and its mechanism of activation in HAE.
METHODS We assessed the expression of NLRP3, caspase-1, interleukin (IL)-1β, and IL-18 in the marginal zone and corresponding normal liver of 60 patients with HAE. A rat model of HAE was employed to investigate the role of the NLRP3 inflammasome in the marginal zone of HAE. Transwell experiments were conducted to investigate the effect of Echinococcus multilocularis (E. multilocularis) in stimulating Kupffer cells and hepatocytes. Furthermore, immunohistochemistry, Western blotting, and enzyme-linked immunosorbent assay were used to evaluate NLRP3, caspase-1, IL-1β, and IL-18 expression; flow cytometry was used to detect apoptosis and reactive oxygen species (ROS).
RESULTS NLRP3 inflammasome activation was significantly associated with ROS. Inhibition of ROS production decreased NLRP3-caspase-1-IL-1β pathway activation and mitigated hepatocyte damage and inflammation.
CONCLUSION E. multilocularis induces hepatocyte damage and inflammation by activating the ROS-mediated NLRP3-caspase-1-IL-1β pathway in Kupffer cells, indicating that ROS may serve as a potential target for the treatment of HAE.
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Affiliation(s)
- Cai-Song Chen
- Research Center for High Altitude Medicine of Qinghai University, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai Province, China
| | - Yao-Gang Zhang
- Qinghai Province Research Key Laboratory for Echinococcosis, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai Province, China
| | - Hai-Jiu Wang
- Qinghai Province Research Key Laboratory for Echinococcosis, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai Province, China
| | - Hai-Ning Fan
- Department of Hepatobiliary and Pancreatic Surgery, Qinghai Province Research Key Laboratory for Echinococcosis, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai Province, China
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Guo B, Zhao L, Zhao L, Mi R, Zhang X, Wang B, Guo G, Ren Y, Qi W, Zhang Z. Survey and Molecular Characterization of Echinococcus granulosus sensu stricto from Livestock and Humans in the Altai Region of Xinjiang, China. Pathogens 2023; 12:pathogens12010134. [PMID: 36678482 PMCID: PMC9866323 DOI: 10.3390/pathogens12010134] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/17/2022] [Revised: 12/29/2022] [Accepted: 01/05/2023] [Indexed: 01/14/2023] Open
Abstract
Cystic echinococcosis (CE), caused by the metacestode Echinococcus granulosus sensu stricto (s.s.), is an important zoonotic parasite, endemic in the Altai region of China. It is a serious human health risk and causes livestock losses. To evaluate the prevalence, genetic variation, and population structure of CE, 2898 sheep and 703 cattle were examined from October 2019 to mid-February 2020 in the Altai region (Altai, Habahe, Fuhai, and Buerjin). Sheep had an infection rate of 4.52% (131/2898) and cattle had an infection rate of 4.84% (34/703). In total, 180 cyst isolates were obtained, including 131 sheep, 34 cattle, and 15 from CE human patients. The cysts were investigated using mitochondrial cytochrome C oxidase subunit 1 (cox1). Polymerase Chain Reaction (PCR) results showed that, among the two genotypes of E. granulosus s.s., there were 22 different haplotypes (Haps). Phylogenetic analysis and parsimony network indicated that seventeen (77.27%) Haps belonged to the sheep strain (G1 genotype) and five Haps (22.73%) belonged to the buffalo strain (G3 genotype). Hap3 was the most common haplotype (65.00%, 112/180), which belongs to the G1 genotype. Hap18−Hap22 were found in human samples, indicating that sheep and cattle reservoirs of human CE. Molecular diversity indices revealed the high levels of haplotype diversity and relatively low levels of nucleotide diversity. Tajima’s D and Fu’s Fs tests displayed that the Altai population had a significant deviation from neutrality. Based on pairwise fixation index (Fst) values, a low level of genetic differentiation was found between the populations of E. granulosus s.s. isolated from different regions. The present survey findings represent an epidemiological survey of CE in the Altai region where there were two genotypes simultaneously and will provide more information on the genetic structure of E. granulosus s.s. within this region.
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Affiliation(s)
- Baoping Guo
- State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, The First Affiliated Hospital of Xinjiang Medical University, No.137, Liyushan Road, Urumqi 830054, China
| | - Li Zhao
- Veterinary Research Institute, Xinjiang Academy of Animal Sciences, No. 726, Dongrong Street, Urumqi 830013, China
| | - Lu Zhao
- College of Life Sciences, Shaanxi Normal University, No. 620 Xi Chang‘an Avenue, Xi’an 710119, China
| | - Rongsheng Mi
- Key Laboratory of Animal Parasitology of Ministry of Agriculture, Laboratory of Quality and Safety Risk Assessment for Animal Products on Biohazards (Shanghai) of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
| | - Xu Zhang
- Veterinary Research Institute, Xinjiang Academy of Animal Sciences, No. 726, Dongrong Street, Urumqi 830013, China
| | - Bingjie Wang
- Veterinary Research Institute, Xinjiang Academy of Animal Sciences, No. 726, Dongrong Street, Urumqi 830013, China
| | - Gang Guo
- State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, The First Affiliated Hospital of Xinjiang Medical University, No.137, Liyushan Road, Urumqi 830054, China
| | - Yuan Ren
- State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, The First Affiliated Hospital of Xinjiang Medical University, No.137, Liyushan Road, Urumqi 830054, China
| | - Wenjing Qi
- State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, The First Affiliated Hospital of Xinjiang Medical University, No.137, Liyushan Road, Urumqi 830054, China
| | - Zhuangzhi Zhang
- Veterinary Research Institute, Xinjiang Academy of Animal Sciences, No. 726, Dongrong Street, Urumqi 830013, China
- Correspondence:
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Shabani M, Solhjoo K, Taghipour A, Jahromi AS, Karami S, Armand B. The occurrence of cystic echinococcosis in slaughtered livestock in Jahrom, south of Iran. Parasite Epidemiol Control 2022; 19:e00274. [PMID: 36118047 PMCID: PMC9475307 DOI: 10.1016/j.parepi.2022.e00274] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/01/2022] [Revised: 07/09/2022] [Accepted: 09/04/2022] [Indexed: 11/21/2022] Open
Abstract
Cystic echincoccosis (CE) is a major medical and veterinary concern in the world, especially in Iran. Domestic intermediate hosts are an important reservoir for the disease spread. The purpose of this study was to determine the prevalence of CE in slaughtered livestock in Jahrom, south of Iran. In this cross-sectional study, a total of 3074 animals (2325 (75.63%) goats, 423 (13.76%) sheep, and 326 (10.60%) cattle) were inspected macroscopically for CE. In this regard, a questionnaire about the age, sex, infected organ, number of cysts, and cyst fertility was accomplished for each animal. Moreover, PCR was applied by using the cytochrome c oxidase I (COX1) and NADH dehydrogenase subunit 1 (nad1) fragments of parasite mitochondrial genomes on some positive samples. Prevalence of CE in sheep, cattle, and goats was 11.34% (48/423), 11.04% (36/326), and 2.79% (65/2325), respectively. In all livestock, CE was more common in females than males. Moreover, the highest infection rate was observed in the age group of more than 72 months. Considering the fertility of cysts, the ratio of the number of fertile cysts to total cysts in sheep and goats were 83.3% (40/48) and 80% (52/65), respectively. All hydatid cysts were infertile in cattle. With regard to the location of the cyst on internal organs, the most were observed in the lungs and liver. Regarding the intensity of infection, 1–5, 6–9 and ≥ 10 cysts were detected in 78.52% (117/149), 6.71% (10/149) and 10.73% (16/149) of infected livestock, respectively. In all livestock, hydatid cyst with a diameter of 1–5 cm was the most frequent with 71.81% (107/149). All 149 cyst samples were subjected to PCR. Among them, a total of 18 samples (six samples of each animal) were prepared for sequencing. G6 was the most dominant. According to the results of present study and the relatively high prevalence of CE in slaughtered livestock in Jahrom, health policy makers, health authorities, and experts should make effective approach in this regard, and implement careful inspections.
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Affiliation(s)
- Manoochehr Shabani
- Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran.,Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran
| | - Kavous Solhjoo
- Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran.,Department of Medical Parasitology and Mycology, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran
| | - Ali Taghipour
- Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran
| | | | - Saina Karami
- Department of Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
| | - Belal Armand
- Institute of Precision Medicine, Medical and Life Sciences Faculty, Furtwangen University, Furtwangen, Germany
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Yang XB, Meng XZ, Zhao Y, Zhao JP, Chen C, Qin Y, Zhang Y, Zhao Q. Meta-analysis of the prevalence of bovine cystic echinococcosis in China during decade. Res Vet Sci 2022; 152:465-475. [PMID: 36150257 DOI: 10.1016/j.rvsc.2022.09.004] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/21/2022] [Revised: 07/29/2022] [Accepted: 09/03/2022] [Indexed: 11/25/2022]
Abstract
BACKGROUND Cystic echinococcosis (CE) is a zoonotic parasitic disease caused by Echinococcus granulosus sensu lato (E. granulosus s.l.) larvae. It is mainly prevalent in western agricultural and pastoral areas in China, seriously affecting the development of public health and animal husbandry. METHODS This study conducted a systematic evaluation and meta-analysis of bovine CE in China in the past 10 years (2011-2020). Five databases (PubMed, ScienceDirect, CNKI, Wanfang Data, VIP Chinese Journal Database) were used to collect the relevant references, and finally 77 published articles were obtained. RESULTS The total prevalence of bovine CE was estimated to be 9.62% during decade, and decreased year by year after reaching its peak in 2014 (18.75%). The highest E. granulosus s.l. infection rate was found in Qinghai province (14.79%). The infection rate of cattle >4 years old (15.84%) was significantly higher than that of other age groups, and cattle raised by grazing was significantly higher than that of other methods (17.00%). In the groups of geographical and climatic factors, the prevalence of CE was higher in regions with high-altitude (20.76%), cold (20.76%) and rainy areas (11.33%). CONCLUSION This study found that old age and grazing were two important factors for the high incidence of bovine CE. The prevalence of CE is higher in low temperature, rainy and high altitude areas, where may be more suitable for the survival of E. granulosus s.l. eggs.
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Affiliation(s)
- Xin-Bo Yang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, Jilin 130118, PR China
| | - Xiang-Zhu Meng
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, Jilin 130118, PR China
| | - Yan Zhao
- Wenzhou Animal Disease Prevention and Control Center, Wenzhou, Zhejiang 325000, PR China
| | - Jin-Ping Zhao
- College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong 266109, PR China
| | - Chao Chen
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, Jilin 130118, PR China
| | - Ya Qin
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, Jilin 130118, PR China
| | - Yuan Zhang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, Jilin 130118, PR China.
| | - Quan Zhao
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, Jilin 130118, PR China.
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Eaton SL, Murdoch F, Rzechorzek NM, Thompson G, Hartley C, Blacklock BT, Proudfoot C, Lillico SG, Tennant P, Ritchie A, Nixon J, Brennan PM, Guido S, Mitchell NL, Palmer DN, Whitelaw CBA, Cooper JD, Wishart TM. Modelling Neurological Diseases in Large Animals: Criteria for Model Selection and Clinical Assessment. Cells 2022; 11:cells11172641. [PMID: 36078049 PMCID: PMC9454934 DOI: 10.3390/cells11172641] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/25/2022] [Revised: 08/19/2022] [Accepted: 08/22/2022] [Indexed: 11/16/2022] Open
Abstract
Issue: The impact of neurological disorders is recognised globally, with one in six people affected in their lifetime and few treatments to slow or halt disease progression. This is due in part to the increasing ageing population, and is confounded by the high failure rate of translation from rodent-derived therapeutics to clinically effective human neurological interventions. Improved translation is demonstrated using higher order mammals with more complex/comparable neuroanatomy. These animals effectually span this translational disparity and increase confidence in factors including routes of administration/dosing and ability to scale, such that potential therapeutics will have successful outcomes when moving to patients. Coupled with advancements in genetic engineering to produce genetically tailored models, livestock are increasingly being used to bridge this translational gap. Approach: In order to aid in standardising characterisation of such models, we provide comprehensive neurological assessment protocols designed to inform on neuroanatomical dysfunction and/or lesion(s) for large animal species. We also describe the applicability of these exams in different large animals to help provide a better understanding of the practicalities of cross species neurological disease modelling. Recommendation: We would encourage the use of these assessments as a reference framework to help standardise neurological clinical scoring of large animal models.
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Affiliation(s)
- Samantha L. Eaton
- Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK
- Correspondence: (S.L.E.); (T.M.W.); Tel.: +44-(0)-131-651-9125 (S.L.E.); +44-(0)-131-651-9233 (T.M.W.)
| | - Fraser Murdoch
- Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK
| | - Nina M. Rzechorzek
- Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK
| | - Gerard Thompson
- Centre for Clinical Brain Sciences, University of Edinburgh, Chancellor’s Building, 49 Little France Crescent, Edinburgh EH16 4SB, UK
- Department of Clinical Neurosciences, NHS Lothian, 50 Little France Crescent, Edinburgh EH16 4TJ, UK
| | - Claudia Hartley
- Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK
| | - Benjamin Thomas Blacklock
- Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK
| | - Chris Proudfoot
- The Large Animal Research & Imaging Facility, Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK
| | - Simon G. Lillico
- Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK
| | - Peter Tennant
- The Large Animal Research & Imaging Facility, Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK
| | - Adrian Ritchie
- The Large Animal Research & Imaging Facility, Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK
| | - James Nixon
- The Large Animal Research & Imaging Facility, Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK
| | - Paul M. Brennan
- Translational Neurosurgery, Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh EH16 4SB, UK
| | - Stefano Guido
- Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK
- Bioresearch & Veterinary Services, University of Edinburgh, Chancellor’s Building, 49 Little France Crescent, Edinburgh EH16 4SB, UK
| | - Nadia L. Mitchell
- Faculty of Agriculture and Life Sciences, Lincoln University, P.O. Box 85084, Lincoln 7647, New Zealand
| | - David N. Palmer
- Faculty of Agriculture and Life Sciences, Lincoln University, P.O. Box 85084, Lincoln 7647, New Zealand
| | - C. Bruce A. Whitelaw
- Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK
| | - Jonathan D. Cooper
- Departments of Pediatrics, Genetics, and Neurology, Washington University School of Medicine in St. Louis, 660 S Euclid Ave, St. Louis, MO 63110, USA
| | - Thomas M. Wishart
- Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK
- Correspondence: (S.L.E.); (T.M.W.); Tel.: +44-(0)-131-651-9125 (S.L.E.); +44-(0)-131-651-9233 (T.M.W.)
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Cao Y, Li M, Haihambo N, Zhu Y, Zeng Y, Jin J, Qiu J, Li Z, Liu J, Teng J, Li S, Zhao Y, Zhao X, Wang X, Li Y, Feng X, Han C. Oscillatory properties of class C notifiable infectious diseases in China from 2009 to 2021. Front Public Health 2022; 10:903025. [PMID: 36033737 PMCID: PMC9402928 DOI: 10.3389/fpubh.2022.903025] [Citation(s) in RCA: 11] [Impact Index Per Article: 3.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/23/2022] [Accepted: 07/19/2022] [Indexed: 01/22/2023] Open
Abstract
Background Epidemics of infectious diseases have a great negative impact on people's daily life. How it changes over time and what kind of laws it obeys are important questions that researchers are always interested in. Among the characteristics of infectious diseases, the phenomenon of recrudescence is undoubtedly of great concern. Understanding the mechanisms of the outbreak cycle of infectious diseases could be conducive for public health policies to the government. Method In this study, we collected time-series data for nine class C notifiable infectious diseases from 2009 to 2021 using public datasets from the National Health Commission of China. Oscillatory power of each infectious disease was captured using the method of the power spectrum analysis. Results We found that all the nine class C diseases have strong oscillations, which could be divided into three categories according to their oscillatory frequencies each year. Then, we calculated the oscillation power and the average number of infected cases of all nine diseases in the first 6 years (2009-2015) and the next 6 years (2015-2021) since the update of the surveillance system. The change of oscillation power is positively correlated to the change in the number of infected cases. Moreover, the diseases that break out in summer are more selective than those in winter. Conclusion Our results enable us to better understand the oscillation characteristics of class C infectious diseases and provide guidance and suggestions for the government's prevention and control policies.
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Affiliation(s)
- Yanxiang Cao
- The National Clinical Research Center for Mental Disorders & Beijing Key Laboratory of Mental Disorders, Beijing Anding Hospital, Capital Medical University, Beijing, China
- Advanced Innovation Center for Human Brain Protection, Capital Medical University, Beijing, China
| | - Meijia Li
- Faculty of Psychology and Center for Neuroscience, Vrije Universiteit Brussel, Brussels, Belgium
| | - Naem Haihambo
- Faculty of Psychology and Center for Neuroscience, Vrije Universiteit Brussel, Brussels, Belgium
| | - Yuyao Zhu
- College of Environmental Sciences and Engineering, Peking University, Beijing, China
| | - Yimeng Zeng
- State Key Laboratory of Cognitive Neuroscience and Learning & IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, China
| | - Jianhua Jin
- Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
| | - Jinyi Qiu
- School of Artificial Intelligence, Beijing Normal University, Beijing, China
| | - Zhirui Li
- Baoding First Central Hospital, Baoding, China
| | - Jiaxin Liu
- Department of Psychology, University of Washington, Washington, SA, United States
| | - Jiayi Teng
- School of Psychology, Philosophy and Language Science, University of Edinburgh, Edinburgh, United Kingdom
| | - Sixiao Li
- Faculty of Arts, Humanities and Cultures, School of Music, University of Leeds, Leeds, United Kingdom
| | - Yanan Zhao
- China Academy of Chinese Medical Sciences, Institute of Acupuncture and Moxibustion, Beijing, China
| | - Xixi Zhao
- The National Clinical Research Center for Mental Disorders & Beijing Key Laboratory of Mental Disorders, Beijing Anding Hospital, Capital Medical University, Beijing, China
- Advanced Innovation Center for Human Brain Protection, Capital Medical University, Beijing, China
| | - Xuemei Wang
- The National Clinical Research Center for Mental Disorders & Beijing Key Laboratory of Mental Disorders, Beijing Anding Hospital, Capital Medical University, Beijing, China
- Advanced Innovation Center for Human Brain Protection, Capital Medical University, Beijing, China
| | - Yaqiong Li
- The National Clinical Research Center for Mental Disorders & Beijing Key Laboratory of Mental Disorders, Beijing Anding Hospital, Capital Medical University, Beijing, China
- Advanced Innovation Center for Human Brain Protection, Capital Medical University, Beijing, China
| | - Xiaoyang Feng
- Institute of Mental Health, Peking University Sixth Hospital, Beijing, China
| | - Chuanliang Han
- Shenzhen Key Laboratory of Neuropsychiatric Modulation and Collaborative Innovation Center for Brain Science, Guangdong Provincial Key Laboratory of Brain Connectome and Behavior, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Shenzhen–Hong Kong Institute of Brain Science, Shenzhen Fundamental Research Institutions, Shenzhen, China
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Avcioglu H, Bia MM, Balkaya I, Kirman R, Akyuz M, Guven E. Cystic echinococcosis in slaughtered sheep in Erzurum province, Turkey. Acta Trop 2022; 233:106559. [PMID: 35688264 DOI: 10.1016/j.actatropica.2022.106559] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/21/2021] [Revised: 03/03/2022] [Accepted: 06/06/2022] [Indexed: 11/01/2022]
Abstract
Cystic echinococcosis (CE) is an important zoonotic infection caused by the larval stages of the genus Echinococcus. Turkey is a highly endemic region for CE and the disease is one of the major public health problems. The study was aimed to assess the situation of the CE in sheep in Turkey and also to provide data on circulating genotypes in the country. A total of 3319 sheep at slaughter were screened during the study. The prevalence of CE in the study area was 31.7% (1052/3319). The lungs were the most frequently CE infected organ (52.6%, 526/1052). Microscopic examination revealed that overall cyst fertility was 68.1%. Molecular analysis of partial fragments of 12S and COI gene regions were included for 351 selected cyst samples and all of them were identified as E. granulosus sensu stricto. Sequence analysis showed that the predominant genotype in the study areas was G1 (77.1%), and the rest were G3 (22.9%). The prevalence rate of CE in sheep in the study area is lower compared to previous years except for one province. Considering the high cyst fertility rate and the predominance of E. granulosus G1 which is particularly pathogenic to humans, calls for serious control measures like public awareness about the disease, sufficient dog deworming programs, continuity of monitoring the disease should be taken.
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Affiliation(s)
- Hamza Avcioglu
- Department of Parasitology, Faculty of Veterinary Medicine, Atatürk University, Erzurum 25240, Turkey.
| | - Mohammed Mebarek Bia
- Department of Parasitology, Parasitology Research Center and Parasite Resource Bank, Chungbuk National University, School of Medicine, Cheongju 28644, Korea
| | - Ibrahim Balkaya
- Department of Parasitology, Faculty of Veterinary Medicine, Atatürk University, Erzurum 25240, Turkey
| | - Ridvan Kirman
- Department of Parasitology, Faculty of Veterinary Medicine, Atatürk University, Erzurum 25240, Turkey
| | - Muzaffer Akyuz
- Department of Parasitology, Faculty of Veterinary Medicine, Atatürk University, Erzurum 25240, Turkey
| | - Esin Guven
- Department of Parasitology, Faculty of Veterinary Medicine, Atatürk University, Erzurum 25240, Turkey
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Meta-analysis on the prevalence of bovine hydatid disease in China from 2000 to 2021. Microb Pathog 2022; 168:105586. [DOI: 10.1016/j.micpath.2022.105586] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/10/2022] [Revised: 05/08/2022] [Accepted: 05/09/2022] [Indexed: 11/23/2022]
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10
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Khan J, Basharat N, Khan S, Jamal SM, Rahman SU, Shah AA, Khan S, Ali R, Khan SN, Ali I. Prevalence and Molecular Characterization of Cystic Echinococcosis in Livestock Population of the Malakand Division, Khyber Pakhtunkhwa, Pakistan. Front Vet Sci 2021; 8:757800. [PMID: 34746289 PMCID: PMC8567086 DOI: 10.3389/fvets.2021.757800] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/12/2021] [Accepted: 09/20/2021] [Indexed: 11/18/2022] Open
Abstract
Cystic echinococcosis (CE) is a neglected zoonotic disease prevalent in Pakistan, but the genetic diversity of the cestode is largely unexplored in the country. This study investigated the molecular epidemiology of CE infecting the livestock population of the Malakand division, Khyber Pakhtunkhwa, Pakistan. A total of 1,200 livestock, including buffaloes, cattle, goats, and sheep, were examined for echinococcosis from November 2017-2018 at different slaughterhouses in the Malakand division. Hydatid cysts were collected from different organs, and hydatid cyst fluid (HCF) was examined microscopically and used for DNA extraction. The LSU (rrnl) and NAD1 genes were amplified and sequenced. The overall prevalence of CE was 17% (204/1,200), including cows (21.7%), buffaloes (17.4%), goats (10%), and sheep (9.6%). The infection was relatively more prevalent among males (17%) than females (16.9%) and animals of older age (>5 years) (p = 0.710). Liver (63.2%) and lungs (25%) were more affected as compared to kidneys (6.8%) and heart (4.9%). HCF analysis indicated that 52.0% of the cysts were sterile and (48.0%) were fertile. Sequencing and phylogenetic analyses confirmed 80.0% of the isolates as Echinococcus granulosus sensu stricto (G1-G3) in all animal species, while Echinococcus equinus (G4) and Echinococcus ortleppi (G5) were present in buffaloes. The present study concluded that CE is prevalent in the livestock population of Malakand. Besides E. granulosus s. s. (G1-G3), E. ortleppi genotype (G5) and E. equinus (G4) in livestock were also reported.
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Affiliation(s)
- Jadoon Khan
- Department of Biosciences, COMSATS University, Islamabad, Pakistan
- Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan
| | - Nosheen Basharat
- Department of Biosciences, COMSATS University, Islamabad, Pakistan
| | - Salman Khan
- Department of Biotechnology, University of Malakand, Chakdara, Pakistan
| | | | - Sadeeq Ur Rahman
- College of Veterinary Science and Animal Husbandry, Abdul Wali Khan University, Mardan, Pakistan
| | - Aamer Ali Shah
- Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan
| | - Sanaullah Khan
- Department of Zoology, University of Peshawar, Peshawar, Pakistan
| | - Rehman Ali
- Department of Zoology, Faculty of Biological Sciences, Kohat University of Science and Technology, Kohat, Pakistan
| | - Shahid Niaz Khan
- Department of Zoology, Faculty of Biological Sciences, Kohat University of Science and Technology, Kohat, Pakistan
| | - Ijaz Ali
- Department of Biosciences, COMSATS University, Islamabad, Pakistan
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11
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Fu MH, Wang X, Han S, Guan YY, Bergquist R, Wu WP. Advances in research on echinococcoses epidemiology in China. Acta Trop 2021; 219:105921. [PMID: 33878307 DOI: 10.1016/j.actatropica.2021.105921] [Citation(s) in RCA: 25] [Impact Index Per Article: 6.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/20/2020] [Revised: 04/10/2021] [Accepted: 04/12/2021] [Indexed: 12/11/2022]
Abstract
Echinococcoses are serious zoonotic diseases in China's vast, western and north-western pastoral areas that has one of the highest prevalence in the world. The two most common forms, cystic echinococcosis (CE) and alveolar echinococcosis (AE), are co-epidemic in some areas causing a grave threat to people's health and economic development. Echinococcus spp. are transmitted through domestic, sylvatic and mixed cycles involving many kinds of host. Successful transmission requires a favourable environment for the growth of the parasites and survival of their eggs, while the unique customs and religious beliefs in the endemic areas pose a challenge to the prevention and control of these parasites. Based on previous epidemiological studies, this paper reviews the particular factors affecting the transmission of Echinococcus parasites in China, with a focus on biological (parasite genotype and the species, age, sex and density of hosts), environmental (landscape and climate) and social (age, gender, ethnicity, education, occupation, life style, cultural customs, living conditions and hygiene practices of humans in the endemic areas). These three factors interact with each other and jointly determine the parasites' transmission intensity, the study of which supports the formulation of the strategies and measures that are significant for control of these infections.
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12
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Vaisi-Raygani A, Mohammadi M, Jalali R, Salari N, Hosseinian-Far M. Prevalence of cystic echinococcosis in slaughtered livestock in Iran: a systematic review and meta-analysis. BMC Infect Dis 2021; 21:429. [PMID: 33962578 PMCID: PMC8103583 DOI: 10.1186/s12879-021-06127-2] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/08/2020] [Accepted: 04/29/2021] [Indexed: 11/10/2022] Open
Abstract
BACKGROUND Hydatidosis is a zoonotic disease and has a great general and economic health importance in both developed and developing countries. Therefore, this systematic and meta-analytic study was conducted to determine the prevalence of cystic echinococcosis in slaughtered livestock in Iran. METHODS The present study was conducted as a systematic review and meta-analysis. The SID & Magiran, MEDLINE (PubMed), ScienceDirect, Scopus, and Google Scholar databases were searched with a view to selecting relevant research works. As a result, 31 articles published from April 1970 to April 2020 were selected. The heterogeneity of the studies was assessed using the I2 index. Data analysis was conducted within the Comprehensive Meta-Analysis software (CMA) v.3.0 (Biostat, Englewood, NJ, USA) and Arc map (ArcGIS 10.3) software. RESULTS The heterogeneity of the studies was evaluated using the I2 test which value was 99% showing a high heterogeneity in the studies. The results of publication bias in studies were evaluated by the Egger test, which were not statistically significant (P = 0.144). The overall prevalence of cystic echinococcosis in slaughtered livestock in Iran is 13.9% (95%CI: 10.7-17.7%). The results of the meta-regression analysis indicate the increasing trend of the hydatid cyst prevalence with the increase of sample size and publication year (P < 0.05). CONCLUSION According to the results of this study and the relatively high prevalence of cystic echinococcosis in slaughtered livestock in Iran, health policy makers should make effective decisions in this regard, and implement careful inspections and interventions by experts and health authorities.
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Affiliation(s)
- Aliakbar Vaisi-Raygani
- Department of Nursing, School of Nursing and Midwifery, Kermanshah University of Medical Sciences, Kermanshah, Iran
| | - Masoud Mohammadi
- Department of Nursing, School of Nursing and Midwifery, Kermanshah University of Medical Sciences, Kermanshah, Iran
| | - Rostam Jalali
- Department of Nursing, School of Nursing and Midwifery, Kermanshah University of Medical Sciences, Kermanshah, Iran
| | - Nader Salari
- Department of Biostatistics, School of Health, Kermanshah University of Medical Sciences, Kermanshah, Iran
| | - Melika Hosseinian-Far
- Department of Food Science & Technology, Faculty of Agriculture, Ferdowsi University of Mashhad (FUM), Mashhad, Iran
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13
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Anvari D, Pourmalek N, Rezaei S, Fotovati A, Hosseini SA, Daryani A, Spotin A, Sarvi S, Hosseini M, Narouei MR, Kalkali M, Pendar F, Gholami S. The global status and genetic characterization of hydatidosis in camels ( Camelus dromedarius): a systematic literature review with meta-analysis based on published papers. Parasitology 2021; 148:259-273. [PMID: 32940199 PMCID: PMC11010127 DOI: 10.1017/s0031182020001705] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/16/2020] [Revised: 08/24/2020] [Accepted: 09/10/2020] [Indexed: 02/07/2023]
Abstract
Hydatidosis is a potential zoonotic helminthic disease affecting a broad spectrum of mammals, including humans, worldwide. The current review was conducted to investigate the genotypic status and prevalence of hydatid disease in camels across the world. For the purpose of the study, the articles addressing the worldwide prevalence of hydatidosis in camels were searched in several English language databases. The search process resulted in the inclusion of 122 papers. Based on the data presented in the reviewed articles, the pooled prevalence of hydatid disease in camels across the world was measured at 23.75% (95% CI 20.15-27.55). Moreover, the subgroup analysis demonstrated significant differences in the overall prevalence of hydatidosis among camels based on year, geographic area, climate parameters, camel population, gender, infected organ, fertility rate of the cyst and laboratory diagnostic technique. Furthermore, the Echinococcus granulosus genotypes identified in camels with hydatidosis included G1, G2, G3, G1-G3, G5, G6, G7, G6-G7 and G6-G10, with G6 being the most common genotype throughout the world. The data obtained from the current study are central to the better conceptualization of the biological and epidemiological characteristics of E. granulosus s.l. genotypes around the world, which can be helpful in the planning and adoption of more comprehensive control strategies.
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Affiliation(s)
- Davood Anvari
- Student Research Committee, Mazandaran University of Medical Science, Sari, Iran
- Toxoplasmosis Research Center, Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
- School of Medicine, Iranshahr University of Medical Sciences, Iranshahr, Iran
| | - Nima Pourmalek
- Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Karaj, Iran
| | - Saeed Rezaei
- Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Karaj, Iran
| | - Amir Fotovati
- Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
| | - Seyed Abdollah Hosseini
- Toxoplasmosis Research Center, Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
| | - Ahmad Daryani
- Toxoplasmosis Research Center, Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
| | - Adel Spotin
- Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
| | - Shahabeddin Sarvi
- Toxoplasmosis Research Center, Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
| | - Mona Hosseini
- Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
| | | | - Mohammad Kalkali
- Student Research Committee, Iranshahr University of Medical Sciences, Iranshahr, Iran
| | - Faezeh Pendar
- Student Research Committee, Iranshahr University of Medical Sciences, Iranshahr, Iran
| | - Shirzad Gholami
- Toxoplasmosis Research Center, Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
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14
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Ahmed AB, Ras R, Mahmoud AF, El-Ghazaly E, Widmer G, Dahshan H, Elsohaby I. Prevalence and bacterial isolation from hydatid cysts in dromedary camels ( Camelus dromedarius) slaughtered at Sharkia abattoirs, Egypt. J Parasit Dis 2020; 45:236-243. [PMID: 33746409 DOI: 10.1007/s12639-020-01300-x] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/29/2020] [Accepted: 10/23/2020] [Indexed: 11/25/2022] Open
Abstract
Cystic echinococcosis (CE) is a severe neglected zoonotic parasitic disease caused by the larval stage of the dog tapeworm, Echinococcus granulosus. The objectives of this study were to determine the prevalence of hydatid cysts in dromedary camels (Camelus dromedarius) at Sharkia province, Egypt and investigate the occurrence of bacteria in hydatid fluid. A total of 6416 dromedary camels slaughtered in five abattoirs in Sharkia province, Egypt during the period from January and December 2018 were investigated for the presence of hydatid cysts. Furthermore, the bacterial species in 10 hydatid fluid isolated from lungs and livers was identified. The current findings revealed that the prevalence of hydatid cysts was 3.7%. Among those, the infection rate in lungs was 78.2%, which was significantly higher than hepatic infections (21.8%). The prevalence of hydatid cysts was the highest in winter (7.4%) and the lowest in spring (1.5%). The most common bacterial species found inside hydatid fluid collected from lungs were Salmonella spp., Staphylococcus spp., Enterococci and Pseudomonas spp. Meanwhile, Staphylococcus spp. were isolated from hepatic hydatid fluid. In conclusion, hydatid cysts infection is prevalent in dromedary camels in Sharkia province, Egypt as well as various aerobic and anaerobic bacterial species were isolated from hydatid fluid from camel lungs and livers.
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Affiliation(s)
| | - Refaat Ras
- Department of Parasitology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
| | - Abdallah F Mahmoud
- Food Control Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
| | - Emad El-Ghazaly
- Food Control Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
| | - Giovanni Widmer
- Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA 01536 USA
| | - Hesham Dahshan
- Department of Veterinary Public Health, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
| | - Ibrahim Elsohaby
- Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
- Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, PEI C1A 4P3 Canada
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15
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Alvi MA, Ohiolei JA, Li L, Saqib M, Hussain MH, Tayyab MH, Ghafoor M, Qamar W, Faridi NY, Alvi AA, Fu BQ, Yan HB, Jia WZ. In-House Developed ELISA Indicates High Prevalence of Anti- Echinococcus granulosus IgG in Sheep Population-An Update from Pakistan. Pathogens 2020; 9:pathogens9110905. [PMID: 33137933 PMCID: PMC7693474 DOI: 10.3390/pathogens9110905] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/06/2020] [Revised: 10/23/2020] [Accepted: 10/27/2020] [Indexed: 01/25/2023] Open
Abstract
Cystic echinococcosis (CE) is a World Health Organization (WHO)-listed neglected tropical farm economy jeopardizing and public health concern disease. This study was aimed at furnishing sero-epidemiological baseline data of CE in sheep in Pakistan, where data are non-existent. For this purpose, two sheep-rich provinces of Pakistan were selected, and 728 sheep sera were collected using probability proportional to size (PPS) statistical technique. Epidemiological information was recorded on a questionnaire for the estimation of potential risk factors. The serum samples were analyzed for IgG antibodies against Echinococcus granulosus using an in-house-developed EgAgB-based ELISA kit. The overall seroprevalence recorded was 21.98% (160/728) in the tested sheep, suggesting higher seropositivity in sheep from Punjab (23.73%) as compared to Khyber Pakhtunkhwa (KPK) (19.04%). The overall apparent prevalence observed by this ELISA method was almost similar to the calculated true prevalence (21.77%). Prevalence was significantly different (p < 0.05) among sheep from different districts. Higher prevalence was found in females (22.54%, OR 1.41), age group > 5 years (29.66%, OR 1.64), crossbreeds (42.85%, OR 2.70), and sheep with pasture access (25.96%, OR 3.06). Being in age group > 5 years and having pasture access were the factors significantly associated with seropositivity (p < 0.05). This study provides serological evidence of E. granulosus infection in sheep and can be used as a model for ante-mortem screening of the sheep globally.
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Affiliation(s)
- Mughees Aizaz Alvi
- State Key Laboratory of Veterinary Etiological Biology, National Professional Laboratory of Animal Hydatidosis, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
| | - John Asekhaen Ohiolei
- State Key Laboratory of Veterinary Etiological Biology, National Professional Laboratory of Animal Hydatidosis, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
| | - Li Li
- State Key Laboratory of Veterinary Etiological Biology, National Professional Laboratory of Animal Hydatidosis, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
| | - Muhammad Saqib
- Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad 38400, Pakistan
| | | | - Muhammad Haleem Tayyab
- Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad 38400, Pakistan
| | - Muzafar Ghafoor
- Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad 38400, Pakistan
| | - Warda Qamar
- Department of Pathobiology, University of Veterinary and Animal Sciences, Lahore Sub-Campus Jhang 12-Km Chiniot Road, Jhang 35200, Pakistan
| | - Noman Yousaf Faridi
- Punjab Livestock and Dairy Development, Government of Punjab, Chiniot 35400, Pakistan
| | | | - Bao-Quan Fu
- State Key Laboratory of Veterinary Etiological Biology, National Professional Laboratory of Animal Hydatidosis, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
| | - Hong-Bin Yan
- State Key Laboratory of Veterinary Etiological Biology, National Professional Laboratory of Animal Hydatidosis, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
| | - Wan-Zhong Jia
- State Key Laboratory of Veterinary Etiological Biology, National Professional Laboratory of Animal Hydatidosis, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
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16
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Zeng X, Guan Y, Wu W, Wang L, Cai H, Fang Q, Yu S, Zheng C. Analysis of Factors Influencing Cystic Echinococcosis in Northwest Non-Qinghai Tibetan Plateau Regions of China. Am J Trop Med Hyg 2020; 102:567-573. [PMID: 31933454 DOI: 10.4269/ajtmh.18-0703] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/01/2023] Open
Abstract
Cystic echinococcosis (CE) is an important health problem in northwest non-Qinghai Tibetan Plateau regions of China. Understanding CE risk factors may help in formulating effective prevention and control measures. In the current study, negative binomial regression was used to analyze CE-related factors using prevalence data from 147 counties (cities, districts, and banners) in northwest non-Qinghai Tibetan Plateau regions of China gathered from a national survey conducted in 2012, county economic data, and livestock production data for 2012. The negative binomial regression models indicated Echinococcus granulosus infection rate in livestock (odds ratio [OR]: 1.0471, 95% CI: 1.0128-1.0825, P = 0.0067), the mean number of dogs per family (OR: 3.0589, 95% CI: 1.5116-6.1900, P = 0.0019), the mean number of sheep per family (OR: 1.0431, 95% CI: 1.0027-1.0852, P = 0.0365), and the source of drinking water (OR: 0.9808, 95% CI: 0.9627-0.9992, P = 0.0413) were correlated with human CE prevalence, whereas altitude, surface temperature, economy, and mean number of cattle per family had a negative association. We found that the positive rate of dog stool coproantigen of Echinococcus was associated with human CE prevalence using the univariate analysis but not after controlling for covariates including the number of dogs per family. This might be partially due to the deworming campaigns that were carried out in most of the researched areas. Therefore, the positive rate of dog stool coproantigen of Echinococcus could not reflect the dog infection rate under natural conditions. These findings indicate that dogs and livestock play the most important role in CE transmission. Further studies should be carried out at the township or village level.
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Affiliation(s)
- Xiangman Zeng
- National Institute of Parasitic Disease China CDC, Shanghai, China.,WHO Collaborating Centre for Tropical Diseases, Shanghai, China.,Chinese Center for Tropical Diseases Research, Shanghai, China.,HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, China.,National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, China
| | - Yayi Guan
- Chinese Center for Tropical Diseases Research, Shanghai, China.,National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, China.,WHO Collaborating Centre for Tropical Diseases, Shanghai, China.,National Institute of Parasitic Disease China CDC, Shanghai, China
| | - Weiping Wu
- Chinese Center for Tropical Diseases Research, Shanghai, China.,National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, China.,WHO Collaborating Centre for Tropical Diseases, Shanghai, China.,National Institute of Parasitic Disease China CDC, Shanghai, China
| | - Liying Wang
- WHO Collaborating Centre for Tropical Diseases, Shanghai, China.,National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, China.,Chinese Center for Tropical Diseases Research, Shanghai, China.,National Institute of Parasitic Disease China CDC, Shanghai, China
| | - Huixia Cai
- Department of Parasite Control, Qinghai Provincial Institute for Endemic Diseases Prevention and Control, Xining, China
| | - Qi Fang
- WHO Collaborating Centre for Tropical Diseases, Shanghai, China.,National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, China.,Chinese Center for Tropical Diseases Research, Shanghai, China.,National Institute of Parasitic Disease China CDC, Shanghai, China
| | - Shicheng Yu
- Chinese Center for Disease Control and Prevention, Beijing, China
| | - Canjun Zheng
- Chinese Center for Disease Control and Prevention, Beijing, China
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17
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The current prevalence and diversity of cystic echinococcosis in slaughtered animals in Egypt. J Parasit Dis 2019; 43:711-717. [PMID: 31749543 DOI: 10.1007/s12639-019-01151-1] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/08/2019] [Accepted: 08/21/2019] [Indexed: 02/02/2023] Open
Abstract
Cystic echinococcosis is a potential zoonotic helminthic disease affect a broad spectrum of mammals including humans worldwide. The economic impact of the disease interestingly requires updated gathering information on the prevalence in slaughtered animals. Accordingly, in the current study, 573 camels, 4300 sheep, and 1235 pigs were surveyed in four Egyptian municipal abattoirs. Among those, 62 (10.82%) camels, 33 (0.77%) sheep and 3 (0.24%) pigs had cystic echinococcosis in lungs, livers and spleen. The diversity of cysts revealed that positive-cystic echinococcosis animals from all species were detected in El-Basatin abattoir. In El-Monieb abattoir, sheep only were infected. In El-Waraa and Beni-Suef abattoirs, cysts were seen in camels only. Infected animals included both sexes. In camels, lungs were the most affected organs, while in sheep, livers were the most abundant. In pigs, hydatid cysts were detected only in lungs. Moreover, camel cysts were mostly large-sized (diameter > 10 cm) with smaller cysts are also recorded. In sheep, small-, moderately- and large-sized cysts were equally present. Cysts in pigs were large-sized. Camels showed a higher percentage of fertile cysts (46.77%; 29/62) followed by sheep (21.21%; 7/33). Calcified/degenerated cysts were less in camels (38.71%; 24/62) than in sheep (75.76%). Non-viable cysts were the less frequent in both species (14.52% in camels and 3.03% in sheep). All cysts recovered from pigs were fertile. In Egypt, governmental agencies and veterinary authorities are asked to potentially eradicate stray dogs (the main definitive host) and towards the proper hygienic disposal of infected offal in abattoirs to minimize the prevalence of cystic echinococcosis.
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18
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Ola-Fadunsin SD, Uwabujo PI, Halleed IN, Richards B. Prevalence and financial loss estimation of parasitic diseases detected in slaughtered cattle in Kwara State, North-central Nigeria. J Parasit Dis 2019; 44:1-9. [PMID: 32174699 DOI: 10.1007/s12639-019-01154-y] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/05/2019] [Accepted: 08/26/2019] [Indexed: 12/01/2022] Open
Abstract
Parasitism is of great concern to the wellbeing, productivity and reproductivity of animals and it causes great financial loss to the economy of any nation. This study was conducted to determine the prevalence and the economic loss from carcass and viscera condemnation due to parasitic diseases in slaughtered cattle in Kwara State, North-central Nigeria. A 16 year (2003-2018) record of parasitic diseases detected in cattle slaughtered at the major abattoirs in Ilorin city was retrieved from the Kwara State Veterinary department of the Ministry of Agriculture and Natural Resources. The total prevalence as well as the yearly, monthly and seasonal prevalence of each of the documented parasitic diseases was determined. The financial losses associated with each of the parasitic diseases were estimated in Nigerian Naira (₦) and US Dollar (USD) considering the market price and weight of viscera condemned. Fasciolosis, hydatidosis, dicrocoeliosis, cysticercosis and oesophagostomiasis were the parasitic disease conditions with fasciolosis (6371/832,001) and cysticercosis (134/832,001) been the most and least prevalent disease conditions respectively. The yearly and monthly prevalence of each disease conditions showed an undefined pattern. There was a significantly difference in the prevalence of the detected parasitic conditions within seasons. The total financial loss associated with these disease conditions was 304,133.82 USD (46,161,433 Nigerian Naira). This study showed that parasitic diseases are of great financial drain to the livestock economy of Nigeria and this study will serve as baseline information on the economic losses associated with parasitic diseases of cattle at slaughter in Nigeria.
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Affiliation(s)
- Shola David Ola-Fadunsin
- 1Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Ilorin, PMB 1515, Ilorin, Kwara State Nigeria
| | - Patricia Isioma Uwabujo
- 1Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Ilorin, PMB 1515, Ilorin, Kwara State Nigeria
| | - Ibrahim Ndaman Halleed
- Veterinary Services Division, Kwara State Ministry of Agriculture and Natural Resources, Ilorin, Kwara State Nigeria
| | - Bukola Richards
- Veterinary Services Division, Kwara State Ministry of Agriculture and Natural Resources, Ilorin, Kwara State Nigeria
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19
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Guo B, Zhang Z, Zheng X, Guo Y, Guo G, Zhao L, Cai R, Wang B, Yang M, Shou X, Zhang W, Jia B. Prevalence and Molecular Characterization of Echinococcus granulosus Sensu Stricto in Northern Xinjiang, China. THE KOREAN JOURNAL OF PARASITOLOGY 2019; 57:153-159. [PMID: 31104407 PMCID: PMC6526214 DOI: 10.3347/kjp.2019.57.2.153] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 08/05/2018] [Accepted: 12/17/2018] [Indexed: 02/04/2023]
Abstract
Echinococcus granulosus is an important zoonotic parasite globally causing cystic echinococcosis (CE) in humans and animals. In this study, prevalence of CE and variation of cox1 gene sequence were analyzed with isolates E. granulosus collected from different areas in northern Xinjiang, China. The survey showed that 3.5% of sheep and 4.1% of cattle were infected with CE. Fragment of cox1 was amplified from all the positive sheep and cattle samples by PCR. In addition, 26 positive samples across the 4 areas were included. The isolates were all E. granulosus sensu stricto (s.s.) containing 15 haplotypes (Hap1-15), and clustered into 2 genotypes, G1 (90.1%, 91/101) and G3 (9.9%, 10/101). Hap1 was the most common haplotype (48.5%, 49/101). Hap9 were found in humans samples, indicating that sheep and cattle reservoir human CE. It is indicate that E. granulosus may impact on control of CE in livestock and humans in the region.
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Affiliation(s)
- Baoping Guo
- College of Animal Science and Technology, Shihezi University, Road Beisi, Shihezi 832003, Xinjiang, PR China
| | - Zhuangzhi Zhang
- Veterinary Research Institute, Xinjiang Academy of Animal Sciences, 726 Street Dongrong, North Gaoxin District, Urumqi 830000, Xinjiang, PR China
| | - Xueting Zheng
- State Key Laboratory for Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asian, First Affiliated Hospital of Xinjiang Medical University, Road Liyushan, Urumqi 830054, Xinjiang, PR China.,Xinjiang Key Laboratory of Medical Animal Model Research, Clinical Medical Research Institute of First Affiliated Hospital of Xinjiang Medical University, Road Liyushan, Urumqi 830011, Xinjiang, PR China
| | - Yongzhong Guo
- The Friendship Hospital of Yili Kazak Autonomous Prefecture, 92 Road Stalin, Yining 835000, Xinjiang, PR China
| | - Gang Guo
- State Key Laboratory for Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asian, First Affiliated Hospital of Xinjiang Medical University, Road Liyushan, Urumqi 830054, Xinjiang, PR China.,Xinjiang Key Laboratory of Medical Animal Model Research, Clinical Medical Research Institute of First Affiliated Hospital of Xinjiang Medical University, Road Liyushan, Urumqi 830011, Xinjiang, PR China
| | - Li Zhao
- Veterinary Research Institute, Xinjiang Academy of Animal Sciences, 726 Street Dongrong, North Gaoxin District, Urumqi 830000, Xinjiang, PR China
| | - Ren Cai
- State Key Laboratory for Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asian, First Affiliated Hospital of Xinjiang Medical University, Road Liyushan, Urumqi 830054, Xinjiang, PR China.,Xinjiang Key Laboratory of Medical Animal Model Research, Clinical Medical Research Institute of First Affiliated Hospital of Xinjiang Medical University, Road Liyushan, Urumqi 830011, Xinjiang, PR China
| | - Bingjie Wang
- Veterinary Research Institute, Xinjiang Academy of Animal Sciences, 726 Street Dongrong, North Gaoxin District, Urumqi 830000, Xinjiang, PR China
| | - Mei Yang
- Basic Medical College of Xinjiang Medical University, Road Liyushan, Urumqi 830011, Xinjiang, PR China
| | - Xi Shou
- State Key Laboratory for Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asian, First Affiliated Hospital of Xinjiang Medical University, Road Liyushan, Urumqi 830054, Xinjiang, PR China.,Xinjiang Key Laboratory of Medical Animal Model Research, Clinical Medical Research Institute of First Affiliated Hospital of Xinjiang Medical University, Road Liyushan, Urumqi 830011, Xinjiang, PR China
| | - Wenbao Zhang
- State Key Laboratory for Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asian, First Affiliated Hospital of Xinjiang Medical University, Road Liyushan, Urumqi 830054, Xinjiang, PR China.,Xinjiang Key Laboratory of Medical Animal Model Research, Clinical Medical Research Institute of First Affiliated Hospital of Xinjiang Medical University, Road Liyushan, Urumqi 830011, Xinjiang, PR China
| | - Bin Jia
- College of Animal Science and Technology, Shihezi University, Road Beisi, Shihezi 832003, Xinjiang, PR China
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20
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Kere OJ, Joseph E, Jessika BL, Maina KJ. Prevalence and monetary loss due to cystic Echinococcosis in slaughter house livestock: A case study of Migori County, Kenya. Parasite Epidemiol Control 2019; 5:e00105. [PMID: 31193569 PMCID: PMC6536491 DOI: 10.1016/j.parepi.2019.e00105] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/05/2019] [Revised: 04/09/2019] [Accepted: 04/13/2019] [Indexed: 11/26/2022] Open
Abstract
Cystic Echinococcosis (CE/Hydatidosis) is a parasitic zoonosis of public health importance that causes considerable economic loss worldwide. The aim of this study was to assess prevalence and monetary loss of CE in livestock slaughtered in Migori County, Kenya. The study was conducted by retrieving and analyzing secondary data over a ten year period (2007–2016) from annual meat inspection reports from sub-county veterinary offices within Migori County. The data included species/number of slaughtered animals and number of organs condemned due to presence of hydatid cyst(s) recorded. The results showed CE prevalence was highest in cattle (5.3%) followed by goats (2.0%), least affected were sheep (0.1%). The overall direct monetary loss was $152,003/year. The study results confirm occurrence of CE in Migori County and demonstrate an emerging new CE focus in Kenya with a significant direct monetary loss, a phenomenon that require serious attention to control the spread of CE in Kenya.
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Affiliation(s)
- Odero Joseph Kere
- Department of Animal Sciences, Jomo Kenyatta University of Agriculture and Technology, P O Box 62, 000-00200, Nairobi, Kenya.,Department of Animal Science, Lilongwe University of Agriculture and Natural Resources, P O Box 219, Lilongwe, Malawi
| | - Erume Joseph
- Department of Biomolecular Resources and Bio laboratory Sciences, Makerere University, P O Box 7062, Kampala, Uganda
| | - Banda Liveness Jessika
- Department of Animal Science, Lilongwe University of Agriculture and Natural Resources, P O Box 219, Lilongwe, Malawi
| | - Kagira John Maina
- Department of Animal Sciences, Jomo Kenyatta University of Agriculture and Technology, P O Box 62, 000-00200, Nairobi, Kenya
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21
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Tianli L, Xifeng W, Zhenzhong T, Lixia W, Xingxing Z, Jun Q, Qingling M, Shasha G, Ying C, Xuepeng C. Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep. THE KOREAN JOURNAL OF PARASITOLOGY 2019; 57:61-67. [PMID: 30840802 PMCID: PMC6409221 DOI: 10.3347/kjp.2019.57.1.61] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 05/01/2018] [Accepted: 01/30/2019] [Indexed: 12/16/2022]
Abstract
Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. coli and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep.
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Affiliation(s)
- Liu Tianli
- College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China
| | - Wang Xifeng
- College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China
| | - Tian Zhenzhong
- College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China
| | - Wang Lixia
- College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China
| | - Zhang Xingxing
- State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, Xinjiang 832000, China
| | - Qiao Jun
- College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China
| | - Meng Qingling
- College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China
| | - Gong Shasha
- College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China
| | - Chen Ying
- College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China
| | - Cai Xuepeng
- State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China
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22
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Moazeni M, Khamesipour F, Anyona DN, Dida GO. Epidemiology of taeniosis, cysticercosis and trichinellosis in Iran: A systematic review. Zoonoses Public Health 2019; 66:140-154. [PMID: 30575317 DOI: 10.1111/zph.12547] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/15/2018] [Revised: 10/25/2018] [Accepted: 11/11/2018] [Indexed: 02/05/2023]
Abstract
BACKGROUND The aim of this review was to establish the current epidemiology of taeniosis, cysticercosis and trichinellosis among humans and animals in Iran by carrying out a comprehensive assessment of published articles reporting on these foodborne zoonotic diseases across the country. METHODS The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guideline was used in the search for relevant published articles reporting on cysticercosis, taeniosis and trichinellosis in Iran using a number of appropriate key words. The search was conducted through PubMed, Web of Science, Google Scholar, SpringerLink, SCOPUS, WHOLIS, FAO and CDC. Published scientific articles including journals, books and book chapters reporting on cysticercosis, taeniosis and trichinellosis in Iran for the period between 1967 and 2018 were selected. RESULTS A total of 37 articles met the search criteria and were incorporated in this review. Of these, 10 (27%) reported on human taeniosis, 15 (40.5%) on cysticercosis (10 on Taenia saginata and five on Taenia spp. cysticercosis) and 12 (32.5%) on trichinellosis. T. saginata was implicated in all human taeniosis cases. All Taenia spp. cysticercosis cases were reported among domesticated pigs and wild animals. A case of neurocysticercosis was reported in a male patient at Shohada Hospital in Tehran. Eleven (91.7%) of the 12 studies reported on trichinellosis among wild animals, while one (8.3%) study detected anti-Trichinella IgG in 8 (2.2%) of the 364 at-risk human beings tested. Nevertheless, most of these studies were carried out in northern Iran. CONCLUSION This review found T. saginata to be the most prevalent and of greater economic and public health significance in Iran. However, T. solium and Trichinella spp. were of little significance to human health. More studies should focus on other regions besides northern Iran.
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Affiliation(s)
- Mohammad Moazeni
- Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
| | - Faham Khamesipour
- Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
- Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
| | - Douglas N Anyona
- School of Environment and Earth Sciences, Maseno University, Kisumu, Kenya
| | - Gabriel O Dida
- Department of Community and Public Health, Technical University of Kenya, Nairobi, Kenya
- School of Public Health and Community Development, Maseno University, Kisumu, Kenya
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23
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Aminzare M, Hashemi M, Faz SY, Raeisi M, Hassanzadazar H. Prevalence of liver flukes infections and hydatidosis in slaughtered sheep and goats in Nishapour, Khorasan Razavi, Iran. Vet World 2018; 11:146-150. [PMID: 29657395 PMCID: PMC5891866 DOI: 10.14202/vetworld.2018.146-150] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/28/2017] [Accepted: 12/26/2017] [Indexed: 12/04/2022] Open
Abstract
Background: Food-borne trematode infections and hydatidosis are endemic diseases caused by helminths in Iran that are responsible for great economic loss and getting public health at risk. Aim: Aim of this study was to determine the prevalence of fasciolosis, dicrocoeliasis, and hydatidosis infections in slaughtered sheep and goats in Nishapour, Khorasan Razavi province of Iran. Materials and Methods: A survey was implemented on 130,107 sheep and goats slaughtered at an abattoir in Nishapour (Neyshbur) city, north central Khorasan Razavi Province, Iran, to determine the prevalence of fascioliasis, dicrocoeliosis and presence of hydatidosis. Results: During a 1-year period of study, among 130,107 of sheep and goats slaughtered at Nishapour abattoir, 1064 and 7124 livers were condemned totally and partially, respectively. A total of 255 (0.19%), 181 (0.12 %), and 7751 (5.95%) of livers were condemned due to cysts of Echinococcus granulosus, flukes of Fasciola spp., and Dicrocoelium dendriticum, respectively. Totally, 1932 (1.48%) lungs were condemned due to hydatidosis. The significant seasonal pattern was seen for fasciolosis, dicrocoeliosis, and hydatidosis, statistically (p<0.01). Conclusion: According to this study, it seems that Neyshabour is considered as an endemic region for Fasciola spp. and D. dendriticum infections and D. dendriticum is the most widespread liver fluke found in sheep and goats.
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Affiliation(s)
- Majid Aminzare
- Department of Food Safety and Hygiene, School of Public Health, Zanjan University of Medical Sciences, Zanjan, Iran
| | - Mohammad Hashemi
- Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
| | - Samaneh Yaghoobi Faz
- Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
| | - Mojtaba Raeisi
- Cereal Health Research Center, Golestan University of Medical Sciences, Gorgan, Iran
| | - Hassan Hassanzadazar
- Department of Food Safety and Hygiene, School of Public Health, Zanjan University of Medical Sciences, Zanjan, Iran
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24
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Zhang RQ, Chen XH, Wen H. Improved experimental model of hepatic cystic hydatid disease resembling natural infection route with stable growing dynamics and immune reaction. World J Gastroenterol 2017; 23:7989-7999. [PMID: 29259374 PMCID: PMC5725293 DOI: 10.3748/wjg.v23.i45.7989] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 07/21/2017] [Revised: 10/06/2017] [Accepted: 10/17/2017] [Indexed: 02/06/2023] Open
Abstract
AIM To investigate a safer way to set up the disease model of cystic echinococcosis without contamination risk and develop a novel experimental murine model of hepatic cystic echinococcosis.
METHODS C57B/6 mice were injected with human protoscolices of three different concentrations via the portal vein. The mice were followed for 10 mo by ultrasound, gross anatomy, and pathological and immunological examinations. The protoscolex migration in the portal vein, hydatid cyst growth, host immune reaction, and hepatic histopathology were examined periodically.
RESULTS The infection rates in the mice in the high, medium, and low concentration groups were 90%, 100%, and 63.6%, respectively. The protoscolices migrated in the portal vein with blood flow, settled in the liver, and developed into orthotopic hepatic hydatid cysts, resembling the natural infection route and course.
CONCLUSION We have established an improved experimental model of hepatic cystic echinococcosis with low biohazard risk but stable growing dynamics and immune reaction. It is especially useful for new anti-parasite medication trials against hydatid disease.
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Affiliation(s)
- Rui-Qing Zhang
- Hepatobiliary and Hydatid Department, Digestive and Vascular Surgery Centre, Xinjiang Key Laboratory of Echinococcosis, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
| | - Xin-Hua Chen
- Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University, Hangzhou 310000, Zhejiang Province, China
- Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, The First Affiliated Hospital, Zhejiang University, Hangzhou 310000, Zhejiang Province, China
- Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University, Hangzhou 310000, Zhejiang Province, China
| | - Hao Wen
- Hepatobiliary and Hydatid Department, Digestive and Vascular Surgery Centre, Xinjiang Key Laboratory of Echinococcosis, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
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25
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Novel Interventional Management of Hepatic Hydatid Cyst with Nanosecond Pulses on Experimental Mouse Model. Sci Rep 2017; 7:4491. [PMID: 28674451 PMCID: PMC5495767 DOI: 10.1038/s41598-017-04873-5] [Citation(s) in RCA: 7] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/05/2017] [Accepted: 05/22/2017] [Indexed: 02/08/2023] Open
Abstract
The nanosecond pulsed electric field (nsPEF) is investigated as an alternative plan for benign hepatic hydatid cyst. Altogether 72 C57B6 mice were included. Normal group (n = 12) had no parasite injection and the other 60 mice were used to induce hydatid cyst in liver by injecting protoscolices in portal vein. The liver hydatid cysts were exposed to nsPEF with different doses and then follow up. The standard surgery was performed as positive control. The hydatid cyst growth was monitored by ultrasound; the morphology was checked by gross anatomy and pathology was tested by H&E stain. In nsPEF-treated groups no hepatic failure nor bleeding were observed. As a comparison, in the surgery group, high post-treatment complications occurred (50%). Significant parasite growth inhibition was seen in high nsPEF dose group as compared with control group (P < 0.05). Pathological analysis confirmed destruction of hydatid cyst with sharp demarcation defined by the electrodes. Laboratory analysis showed nsPEF stimulated a time-dependent infection and recoverable liver function. The traumatic reactions defined by white blood count was significant lower than surgery groups (P < 0.05).Preliminary studies demonstrate nsPEF ablation can be applied on hepatic hydatid by inhibiting parasite growth, destructing the cyst and stimulating infections.
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26
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AZIZI H, KAZEMI B, BANDEHPOUR M, MOHEBALI M, KHAMESIPOUR A, ARYAEIPOUR M, YAGHOOBI H, ROKNI MB. Modulation of the Immune Response to DNA Vaccine Encoding Gene of 8-kDa Subunit of Echinococcus granulosus Antigen B Using Murine Interleukin-12 Plasmid in BALB/c Mice. IRANIAN JOURNAL OF PARASITOLOGY 2016; 11:480-489. [PMID: 28127359 PMCID: PMC5251176] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 02/14/2016] [Accepted: 08/12/2016] [Indexed: 11/25/2022]
Abstract
BACKGROUND The current study was designed to evaluate immune responses induced by DNA vaccines encoding 8-kDa subunit of antigen B (HydI) of Echinococcus granulosus and murine interleukin 12 (IL-12) as genetic adjuvants in BALB/c mice. METHODS Expression plasmid pcDNA3.1 containing HydI (pcHyd1) as vaccine along with the murine interleukin 12 (pcMIL12) as adjuvant were used. Thirty-five mice in the five experimental groups received PBS, empty pcDNA3.1, pcHydІ, pcMIL-12, and pcHydІ+ pcMIL-12 in days zero, 14th and 28th. Two weeks after the last immunization, evaluation of the immune response was performed by evaluating the proliferation of splenic lymphocytes, IFN-γ and IL-4, determination of IgG isotyping titer. RESULTS Mice that received the pcHydI+pcMIL12 exhibited higher levels of lymphocyte proliferation compared to mice that received the pcHydI alone (P<0.001), and produced significantly more IFN-γ in comparison to other groups (P< 0.001). In addition, they produced significantly less IL-4 than mice receiving the PBS and the empty plasmid (P<0.023). The IgG2a levels were clearly higher in pcHydI+pcMIL12 group in comparison with the groups of pcHydI alone, empty plasmid, and PBS. In contrast, IgG1 was elevated in the group of pcHydI. CONCLUSION Co-delivery of IL-12 with DNA encoding 8-kDa subunit of antigen B was effective significantly in inducing the immune response in mice.
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Affiliation(s)
- Hakim AZIZI
- Dept. of Medical Parasitology, Zabol University of Medical Sciences, Zabol, Iran
- Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
| | - Bahram KAZEMI
- Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
- Dept. of Medical Biotechnology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| | - Mojgan BANDEHPOUR
- Dept. of Medical Biotechnology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| | - Mehdi MOHEBALI
- Dept. of Medical Parasitology, Zabol University of Medical Sciences, Zabol, Iran
- Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran
| | - Ali KHAMESIPOUR
- Skin and Leprosy Research Center, Tehran University of Medical Sciences, Tehran, Iran
| | - Mojgan ARYAEIPOUR
- Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
| | - Hajar YAGHOOBI
- Dept. of Medical Biotechnology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| | - Mohammad Bagher ROKNI
- Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
- Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran
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27
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Rahimi M, Sarvi S, Daryani A, Sharif M, Ahmadpour E, Shokri A, Mizani A. Application of multiplex PCR for the simultaneous detection of Taenia spp. from domestic dogs in the north of Iran. Helminthologia 2016. [DOI: 10.1515/helmin-2016-0017] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/15/2022] Open
Abstract
Summary
The family Taeniidae is of great importance in the medical and veterinary fields, particularly in the tropics and subtropics. Identification of eggs of different Taenia spp. in the final host by morphological examination is difficult owing to their similarity. Therefore, a multiplex polymerase chain reaction (PCR) targeting a mitochondrial gene was applied to identify morphologically indistinguishable eggs. Fecal samples from 100 domestic dogs, from the Mazandaran province in Iran, were examined using the flotation/sieving method followed by multiplex PCR. Taeniid eggs were observed in 24 % samples, of which 12 %, 10 %, and 2 % were infected with Echinococcus granulosus, Taenia spp., and both E. granulosus and Taenia spp., respectively. E. multilocularis was absent in these samples. The prevalence of E. granulosus in the examined domestic dogs as definitive hosts in north of Iran was high (14 %). Therefore, people living in this region of Iran are in danger of acquiring hydatid cyst, which is a serious public health problem.
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Affiliation(s)
| | - S. Sarvi
- Toxoplasmosis Research Center, Mazandaran University of Medical Science, Sari, Iran
| | - A. Daryani
- Toxoplasmosis Research Center, Mazandaran University of Medical Science, Sari, Iran
| | - M. Sharif
- Toxoplasmosis Research Center, Mazandaran University of Medical Science, Sari, Iran
| | - E. Ahmadpour
- Infectious and Tropical Diseases Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran
| | - A. Shokri
- Toxoplasmosis Research Center, Mazandaran University of Medical Science, Sari, Iran
| | - A. Mizani
- Toxoplasmosis Research Center, Mazandaran University of Medical Science, Sari, Iran
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28
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First serological study of equine hydatidosis in Iran. J Parasit Dis 2016; 40:1567-1570. [PMID: 27876984 DOI: 10.1007/s12639-015-0729-2] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/13/2015] [Accepted: 09/23/2015] [Indexed: 09/29/2022] Open
Abstract
Hydatidosis, is an important worldwide zoonotic disease caused by larval stages (metacestodes) of tapeworm parasites of the genus Echinococcus. The objective of the present study was to determine the seroprevalence of equine hydatidosis in Iran by latex agglutination test. This study also served to correlate sex and age with mentioned results in cases. Therefore, 193 serum samples were collected from clinically healthy horses at 9 race clubs in Kerman, Yazd and Golestan provinces, Iran. According to the results, antibodies against hydatidosis were detected in 6 sera (3.11 %) among 193 samples. Results showed two male and four female horses were sero-positive against hydatidosis. In conclusion, present study shows that antibodies against hydatidosis have been detected in Iran equine population. Therefore, it seems that Iranian horse clubs should improve their management and health levels to increase their proficiencies.
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