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1
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Liu Y, Meng X, Wang H, Sun Y, Wang SY, Jiang YK, Algradi AM, Naseem A, Kuang HX, Yang BY. Inositol Derivatives with Anti-Inflammatory Activity from Leaves of Solanum capsicoides Allioni. Molecules 2022; 27:molecules27186063. [PMID: 36144793 PMCID: PMC9503535 DOI: 10.3390/molecules27186063] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/28/2022] [Revised: 09/10/2022] [Accepted: 09/14/2022] [Indexed: 11/16/2022] Open
Abstract
Eight new inositol derivatives, solsurinositols A-H (1-8), were isolated from the 70% EtOH extract of the leaves of Solanum capsicoides Allioni. Careful isolation by silica gel column chromatography followed by preparative high-performance liquid chromatography (HPLC) allowed us to obtain analytically pure compounds 1-8. They shared the same relative stereochemistry on the ring but have different acyl groups attached to various hydroxyl groups. This was the first time that inositol derivatives have been isolated from this plant. The chemical structures of compounds 1-8 were characterized by extensive 1D nuclear magnetic resonance (NMR) and 2D NMR and mass analyses. Meanwhile, the in vitro anti-inflammatory activity of all compounds was determined using lipopolysaccharide (LPS)-induced BV2 microglia, and among the isolates, compounds 5 (IC50 = 11.21 ± 0.14 µM) and 7 (IC50 = 14.5 ± 1.22 µM) were shown to have potential anti-inflammatory activity.
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2
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Saranya T, Ramya S, Kavithaa K, Paulpandi M, Cheon YP, Harysh Winster S, Balachandar V, Narayanasamy A. Green Synthesis of Selenium Nanoparticles Using Solanum nigrum Fruit Extract and its Anti-cancer Efficacy Against Triple Negative Breast Cancer. J CLUST SCI 2022. [DOI: 10.1007/s10876-022-02334-2] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/24/2022]
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Antimalarial Activity of Crude Extract and Solvent Fractions of Leaves of Solanum nigrum L. (Solanaceae) against Plasmodium berghei in Mice. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2022. [DOI: 10.1155/2022/3426175] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/17/2022]
Abstract
Background. Current malaria treatment is associated with continued development of drug resistance. Thus, there is a need to develop safe and effective new treatments from different sources. Solanum nigrum L. (Solanaceae) is a plant used for the treatment of malaria in Ethiopian traditional medicine. This study was aimed at evaluating of antimalarial activity of the crude extract and fractions of S. nigrum L. (Solanaceae) leaves against P. berghei infection in mice. Method. Both prophylactic and suppressive models were used in evaluating antimalarial activity using the ANKA Plasmodium strain. In these models, male mice were randomly grouped into eleven groups (n = 5). Mice in group I were given 4% Tween-80, mice from group II up to X were given 100, 200, and 400 mg/kg of plant extract, and the last group (XI) was treated with chloroquine (25 mg/kg). Data were analyzed using one-way ANOVA followed by post hoc Tukey’s multiple comparison test. Results. Crude extract of leaves of S. nigrum showed chemosuppression of 30.68 (
), 42.42 (
), and 50.75% (
) at 100, 200, and 400 mg/kg doses of the extract, respectively. At doses of 100, 200, and 400 mg/kg, the chloroform fraction produced a chemosuppressive effect of 40.15% (
), 53.78% (
), and 65.15% (
) and a chemoprophylactic effect of 42.7% (
), 51.84% (
), and 67.17% (
) when compared with negative control. In the suppressive model, the ethyl acetate fraction demonstrated a mean chemosuppression of 56.81% (
), 65.9% (
), and 70.83% (
). Similarly, in the prophylactic model, the fraction showed suppression of 42.70% (
), 53.11% (
), and 71.03% (
) at 100, 200, and 400 mg/kg doses. On the acute oral toxicity test, the extracts were safe at 2 g/kg dose. Conclusion. S. nigrum L. has antimalarial activity and supports the traditional medical practice.
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4
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Krithika R, Verma RJ. Solanum nigrum confers protection against CCl4-induced experimental hepatotoxicity by increasing hepatic protein synthesis and regulation of energy metabolism. CLINICAL PHYTOSCIENCE 2019. [DOI: 10.1186/s40816-018-0096-5] [Citation(s) in RCA: 16] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 11/10/2022] Open
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5
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Ahmad R. Steroidal glycoalkaloids from Solanum nigrum target cytoskeletal proteins: an in silico analysis. PeerJ 2019; 7:e6012. [PMID: 30627484 PMCID: PMC6321755 DOI: 10.7717/peerj.6012] [Citation(s) in RCA: 14] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/05/2018] [Accepted: 10/26/2018] [Indexed: 12/03/2022] Open
Abstract
Background Solanum nigrum (black nightshade; S. nigrum), a member of family Solanaceae, has been endowed with a heterogeneous array of secondary metabolites of which the steroidal glycoalkaloids (SGAs) and steroidal saponins (SS) have vast potential to serve as anticancer agents. Since there has been much controversy regarding safety of use of glycoalkaloids as anticancer agents, this area has remained more or less unexplored. Cytoskeletal proteins like actin play an important role in maintaining cell shape, synchronizing cell division, cell motility, etc. and along with their accessory proteins may also serve as important therapeutic targets for potential anticancer candidates. In the present study, glycoalkaloids and saponins from S. nigrum were screened for their interaction and binding affinity to cytoskeletal proteins, using molecular docking. Methods Bioactivity score and Prediction of Activity Spectra for Substances (PASS) analysis were performed using softwares Molinspiration and Osiris Data Explorer respectively, to assess the feasibility of selected phytoconstituents as potential drug candidates. The results were compared with two standard reference drugs doxorubicin hydrochloride (anticancer) and tetracycline (antibiotic). Multivariate data obtained were analyzed using principal component analysis (PCA). Results Docking analysis revealed that the binding affinities of the phytoconstituents towards the target cytoskeletal proteins decreased in the order coronin>villin>ezrin>vimentin>gelsolin>thymosin>cofilin. Glycoalkaloid solasonine displayed the greatest binding affinity towards the target proteins followed by alpha-solanine whereas amongst the saponins, nigrumnin-I showed maximum binding affinity. PASS Analysis of the selected phytoconstituents revealed 1 to 3 violations of Lipinski’s parameters indicating the need for modification of their structure-activity relationship (SAR) for improvement of their bioactivity and bioavailability. Glycoalkaloids and saponins all had bioactivity scores between −5.0 and 0.0 with respect to various receptor proteins and target enzymes. Solanidine, solasodine and solamargine had positive values of druglikeness which indicated that these compounds have the potential for development into future anticancer drugs. Toxicity potential evaluation revealed that glycoalkaloids and saponins had no toxicity, tumorigenicity or irritant effect(s). SAR analysis revealed that the number, type and location of sugar or the substitution of hydroxyl group on alkaloid backbone had an effect on the activity and that the presence of α-L-rhamnopyranose sugar at C-2 was critical for a compound to exhibit anticancer activity. Conclusion The present study revealed some cytoskeletal target(s) for S. nigrum phytoconstituents by docking analysis that have not been previously reported and thus warrant further investigations both in vitro and in vivo.
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Affiliation(s)
- Rumana Ahmad
- Department of Biochemisty, Era's Lucknow Medical College and Hospital, Era University, Lucknow, Uttar Pradesh, India
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6
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Gu XY, Shen XF, Wang L, Wu ZW, Li F, Chen B, Zhang GL, Wang MK. Bioactive steroidal alkaloids from the fruits of Solanum nigrum. PHYTOCHEMISTRY 2018; 147:125-131. [PMID: 29306798 DOI: 10.1016/j.phytochem.2017.12.020] [Citation(s) in RCA: 45] [Impact Index Per Article: 6.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/10/2017] [Revised: 12/23/2017] [Accepted: 12/29/2017] [Indexed: 05/25/2023]
Abstract
The investigation of the fruits of Solanum nigrum led to the isolation of four previously undescribed steroidal alkaloids, named solanine A, 7α-OH khasianine, 7α-OH solamargine and 7α-OH solasonine, together with six known ones. The structures of the isolated compounds were elucidated unambiguously by spectroscopic data analyses and chemical methods. Solanine A represents an unusual steroidal alkaloid with an unprecedented 6/5/6/5/5/6 hexacyclic ring system, and its structure was confirmed by X-ray single crystal diffraction analysis. Compounds 2-4 were rare naturally occurring steroidal alkaloid glycosides bearing a hydroxyl group at C-7 position. Solanine A showed the most potent inhibitory activity against the LPS-induced NO production in murine RAW264.7 macrophages with an IC50 value of 3.85 ± 0.71 μM and significant cytotoxicity against MGC803, HepG2 and SW480 cancer cell lines with IC50 values of 6.00 ± 0.52 μM, 9.25 ± 0.49 μM and 6.23 ± 0.26 μM, respectively.
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MESH Headings
- Alkaloids/chemistry
- Alkaloids/isolation & purification
- Alkaloids/pharmacology
- Animals
- Antineoplastic Agents, Phytogenic/chemistry
- Antineoplastic Agents, Phytogenic/isolation & purification
- Antineoplastic Agents, Phytogenic/pharmacology
- Cell Line, Tumor
- Cell Proliferation/drug effects
- Cell Survival/drug effects
- Dose-Response Relationship, Drug
- Drug Screening Assays, Antitumor
- Drugs, Chinese Herbal/chemistry
- Drugs, Chinese Herbal/isolation & purification
- Drugs, Chinese Herbal/pharmacology
- Fruit/chemistry
- Humans
- Lipopolysaccharides/antagonists & inhibitors
- Lipopolysaccharides/pharmacology
- Macrophages/drug effects
- Macrophages/metabolism
- Medicine, Chinese Traditional
- Mice
- Molecular Structure
- Nitric Oxide/antagonists & inhibitors
- Nitric Oxide/biosynthesis
- RAW 264.7 Cells
- Solanum nigrum/chemistry
- Steroids/chemistry
- Steroids/isolation & purification
- Steroids/pharmacology
- Structure-Activity Relationship
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Affiliation(s)
- Xin-Yue Gu
- School of Pharmacy and Bioengineering, Chengdu University, Chengdu, 610106, China; Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China
| | - Xiao-Fei Shen
- Key Laboratory of Birth Defects and Related Diseases of Women and Children (Ministry of Education), Department of Paediatrics, West China Second University Hospital, Sichuan University, Chengdu, 610041, China
| | - Lun Wang
- School of Pharmacy and Bioengineering, Chengdu University, Chengdu, 610106, China; Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.
| | - Zhou-Wei Wu
- Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China
| | - Fu Li
- School of Pharmacy and Bioengineering, Chengdu University, Chengdu, 610106, China; Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China
| | - Bin Chen
- Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China
| | - Guo-Lin Zhang
- Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China
| | - Ming-Kui Wang
- School of Pharmacy and Bioengineering, Chengdu University, Chengdu, 610106, China; Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.
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7
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Two potential uses for silver nanoparticles coated with Solanum nigrum unripe fruit extract: Biofilm inhibition and photodegradation of dye effluent. Microb Pathog 2017; 111:316-324. [DOI: 10.1016/j.micpath.2017.08.039] [Citation(s) in RCA: 33] [Impact Index Per Article: 4.1] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/26/2017] [Revised: 08/26/2017] [Accepted: 08/30/2017] [Indexed: 11/17/2022]
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8
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Hameed A, Ijaz S, Mohammad IS, Muhammad KS, Akhtar N, Khan HMS. Aglycone solanidine and solasodine derivatives: A natural approach towards cancer. Biomed Pharmacother 2017; 94:446-457. [PMID: 28779706 DOI: 10.1016/j.biopha.2017.07.147] [Citation(s) in RCA: 23] [Impact Index Per Article: 2.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/05/2017] [Revised: 07/26/2017] [Accepted: 07/27/2017] [Indexed: 11/30/2022] Open
Abstract
Over the past few years, it was suggested that a rational approach to treat cancer in clinical settings requires a multipronged approach that augments improvement in systemic efficiency along with modification in cellular phenotype leads to more efficient cell death response. Recently, the combinatory delivery of traditional chemotherapeutic drugs with natural compounds proved to be astonishing to deal with a variety of cancers, especially that are resistant to chemotherapeutic drugs. The natural compounds not only synergize the effects of chemotherapeutics but also minimize drug associated systemic toxicity. In this review, our primary focus was on antitumor effects of natural compounds. Previously, the drugs from natural sources are highly precise and safer than drugs of synthetic origins. Many natural compounds exhibit anti-cancer potentials by inducing apoptosis in different tumor models, in-vitro and in-vivo. Furthermore, natural compounds are also found equally useful in chemotherapeutic drug resistant tumors. Moreover, these Phyto-compounds also possess numerous other pharmacological properties such as antifungal, antimicrobial, antiprotozoal, and hepatoprotection. Aglycone solasodine and solanidine derivatives are the utmost important steroidal glycoalkaloids that are present in various Solanum species, are discussed here. These natural compounds are highly cytotoxic against different tumor cell lines. As the molecular weight is concerned; these are smaller molecular weight chemotherapeutic agents that induce cell death response by initiating apoptosis through both extrinsic and intrinsic pathways.
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Affiliation(s)
- Abdul Hameed
- Department of Pharmacy, Faculty of Pharmacy and Alternative Medicines, The Islamia University of Bahawalpur, Bahawalpur, Punjab, 63100, Pakistan
| | - Shakeel Ijaz
- Department of Pharmacy, Faculty of Pharmacy and Alternative Medicines, The Islamia University of Bahawalpur, Bahawalpur, Punjab, 63100, Pakistan
| | - Imran Shair Mohammad
- Department of Pharmaceutics, School of Pharmacy, China Pharmaceutical University, Nanjing, 211198, PR China.
| | | | - Naveed Akhtar
- Department of Pharmacy, Faculty of Pharmacy and Alternative Medicines, The Islamia University of Bahawalpur, Bahawalpur, Punjab, 63100, Pakistan
| | - Haji Muhammad Shoaib Khan
- Department of Pharmacy, Faculty of Pharmacy and Alternative Medicines, The Islamia University of Bahawalpur, Bahawalpur, Punjab, 63100, Pakistan
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Ganesan K, Jayachandran M, Xu B. A critical review on hepatoprotective effects of bioactive food components. Crit Rev Food Sci Nutr 2017; 58:1165-1229. [DOI: 10.1080/10408398.2016.1244154] [Citation(s) in RCA: 28] [Impact Index Per Article: 3.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/06/2023]
Affiliation(s)
- Kumar Ganesan
- Program of Food Science and Technology, Beijing Normal University–Hong Kong Baptist University United International College, Zhuhai, China
| | - Muthukumaran Jayachandran
- Program of Food Science and Technology, Beijing Normal University–Hong Kong Baptist University United International College, Zhuhai, China
| | - Baojun Xu
- Program of Food Science and Technology, Beijing Normal University–Hong Kong Baptist University United International College, Zhuhai, China
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10
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El-Meligy RM, Awaad AS, Soliman GA, Kenawy SA, Alqasoumi SI. Prophylactic and curative anti-ulcerogenic activity and the possible mechanisms of action of some desert plants. Saudi Pharm J 2017; 25:387-396. [PMID: 28344494 PMCID: PMC5357099 DOI: 10.1016/j.jsps.2016.10.008] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/24/2016] [Accepted: 10/08/2016] [Indexed: 12/17/2022] Open
Abstract
The present study aimed to evaluate the anti-ulcerogenic activities and the possible mechanisms of action of seven desert plants from different families. Conyza dioscoridis (L.) Desf. (Asteraceae), Euphorbia hirta L. (Euphorpiaceae), Origanum syriacum L., Salvia lanigera L. (Lamiaceae), Sisymbrium irio L., Solanum nigrum Linn. (Solanaceae) and Solenostemma arghel (Del.) Hayne. (Asclepiadaceae), were tested using prophylactic and curative models of absolute ethanol-induced ulcer, at three doses (125, 250 & 500 mg/kg) of each extract. The investigated extracts possessed dose dependent anti-ulcerogenic activities in both models, with LD50 higher than 5 g/kg. The most effective extracts were C. dioscoridis and S. irio with percent protection of control ulcer; 91.1% and 85.4% respectively. The antisecretory activity of both C. dioscoridis and S. irio appears to be mainly related to the suppression of gastrin release. The in vitro potential radical (DPPH) scavenging activities of the investigated extracts were well supported with the reduction in gastric MDA (50.6% and 43.3%) and enhancing the level of reduced GSH (2.84, 2.59 mg/g tissue) for C. dioscoridis and S. irio respectively. In addition, suppression of the inflammatory mediator TNF-α may be one of the possible mechanisms of action. The alcohol extracts of C. dioscoridis and S. irio showed no alteration on liver and kidney functions. Phytochemical screening of the investigated extracts revealed the presence of flavonoids, tannins and sterols which could be related to the activities.
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Affiliation(s)
- Reham M El-Meligy
- Department of Medicinal and Aromatic Plants, Desert Research Center, Cairo, Egypt
| | - Amani S Awaad
- Department Pharmacognosy, Faculty of Pharmacy, Prince Sattam Bin Abdul Aziz University, Al-Kharj, Saudi Arabia
| | - Gamal A Soliman
- Pharmacology Dept., College of Pharmacy, Al-Kharj University, Al-Kharj, Saudi Arabia
| | - Sanaa A Kenawy
- Pharmacology and Toxicology Department, Faculty of Pharmacy, Cairo University, Egypt
| | - Saleh I Alqasoumi
- Department of Pharmacognosy, College of Pharmacy, King Saud University, Saudi Arabia
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11
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Jinu U, Jayalakshmi N, Sujima Anbu A, Mahendran D, Sahi S, Venkatachalam P. Biofabrication of Cubic Phase Silver Nanoparticles Loaded with Phytochemicals from Solanum nigrum Leaf Extracts for Potential Antibacterial, Antibiofilm and Antioxidant Activities Against MDR Human Pathogens. J CLUST SCI 2016. [DOI: 10.1007/s10876-016-1125-5] [Citation(s) in RCA: 25] [Impact Index Per Article: 2.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/25/2022]
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Zhao X, Qian Y, Li GJ, Tan J. Preventive effects of the polysaccharide of Larimichthys crocea swim bladder on carbon tetrachloride (CCl4)-induced hepatic damage. Chin J Nat Med 2016; 13:521-8. [PMID: 26233842 DOI: 10.1016/s1875-5364(15)30046-7] [Citation(s) in RCA: 14] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/26/2014] [Indexed: 02/03/2023]
Abstract
The aim of the present study was to determine the preventive effects of the polysaccharide of Larimichthys crocea swim bladder (PLCSB) on CCl4-induced hepatic damage in ICR mice. The in vitro preventive effects of PLCSB on CCl4-induced liver cytotoxic effect were evaluated in BRL 3A rat liver cells using the MTT assay. The serum levels of AST, ALT, and LDH in mice were determined using commercially available kits. The levels of IL-6, IL-12, TNF-α, and IFN-γ were determined using ELISA kits. The pathological analysis of hepatic tissues was performed with H and E staining, and the gene and protein expressions were determined by RT-PCR and Western blotting, respectively. PLCSB (20 μg·mL(-1)) could increase the growth of BRL 3A rat liver cells treated with CCl4. The serum levels of AST, ALT, and LDH were significantly decreased when the mice were treated with two doses of PLCSB, compared with the control mice (P < 0.05). PLCSB-treated groups also showed reduced levels of the serum pro-inflammatory cytokines IL-6, IL-12, TNF-α, and IFN-γ. PLCSB could decrease the liver weight, compared to the CCl4-treated control mice. The histopathology sections of liver tissues in the 100 mg·kg(-1) PLCSB group indicated that the animals were recovered well from CCl4 damage, but the 50 mg·kg(-1) PLCSB group showed necrosis to a more serious extent. The 100 mg·kg(-1) PLCSB group showed significantly decreased mRNA and protein expression levels of NF-κB, iNOS, and COX-2, and increased expression of IκB-α compared with the CCl4-treated control group. In conclusion, PLCSB prevented from CCl4-induced hepatic damage in vivo.
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Affiliation(s)
- Xin Zhao
- Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
| | - Yu Qian
- Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
| | - Gui-Jie Li
- Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
| | - Jun Tan
- Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China.
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13
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Rehan HS, Chopra D, Yadav M, Wardhan N, Manak S, Siddiqui KM, Aslam M. Safety and efficacy of Qurse-e-istisqua in chronic hepatitis C infection: an exploratory study. Indian J Pharmacol 2015; 47:72-9. [PMID: 25821315 PMCID: PMC4375823 DOI: 10.4103/0253-7613.150347] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 09/11/2013] [Revised: 11/24/2014] [Accepted: 12/30/2014] [Indexed: 12/02/2022] Open
Abstract
Background: Qurse-e-istisqua (Q-e-I), an Unani medicine commonly prescribed to treat liver disorders. Objectives: To study efficacy and safety of Q-e-I in hepatitis C virus (HCV) infection. Methods: In this randomized double-blind exploratory study, 60 naive patients of HCV infection were assigned to receive either interferonα2a (IFNα2a) (3 mIU, subcutaneous, thrice weekly), ribavirin (RBV) (1000 mg, orally, twice daily in divided doses) and placebo (n = 30) or IFNα2a, RBV and Q-e-I (5 g, orally, thrice daily in divided doses) (n = 30). HCV RNA levels, serum hyaluronic acid (SHA), ultrasound image scoring for fibrosis, liver and renal function test, prothrombin time, were done at the baseline and thereafter periodically. Results: Early virologic response (EVR), end of treatment response (ETR) and sustained virologic response (SVR) were 90%, 96.6% and 90% in the control group and 86.6%, 90.0% and 83.3% in the treatment group. SHA level was lower in the treatment group at the end of the treatment as compared to the control group. Mean end of follow-up ultrasound image scoring for fibrosis in the control and the treatment group was 1.37 ± 0.07 and 1.22 ± 0.06 respectively. Aspartate aminotransferase (AST) levels were significantly lower in the treatment group than the control group at 1-month. Commonly observed adverse drug reactions included fever, hair fall, fatigue, anemia, and diarrhea. Conclusion: Q-e-I was well tolerated and showed anti-fibrotic activity. EVR, ETR and SVR suggested that Q-e-I do not have any anti-HCV activity. Early recovery in AST and inhibition of progress of fibrosis in Q-e-I group was probably due to the anti-inflammatory and antioxidant activity of its ingredients.
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Affiliation(s)
| | - Deepti Chopra
- Department of Pharmacology, HIMSR, Jamia Hamdard, New Delhi, India
| | - Madhur Yadav
- Department of Medicine, Lady Hardinge Medical College, New Delhi, India
| | - Neeta Wardhan
- Department of Pharmacology, University College of Medical Sciences, New Delhi, India
| | - Seema Manak
- Department of Pharmacology, Lady Hardinge Medical College, New Delhi, India
| | - K M Siddiqui
- Central Council for Research in Unani Medicines, AYUSH, Ministry of Health and Family Welfare, Janak Puri, New Delhi, India
| | - Mohd Aslam
- Department Pharmacology Ilmul Advia, faculty of Unani Medicine, Hamdard University, New Delhi, India
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14
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A review of hepatoprotective plants used in saudi traditional medicine. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2014; 2014:890842. [PMID: 25587347 PMCID: PMC4281445 DOI: 10.1155/2014/890842] [Citation(s) in RCA: 43] [Impact Index Per Article: 3.9] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 06/03/2014] [Revised: 09/17/2014] [Accepted: 09/18/2014] [Indexed: 12/12/2022]
Abstract
Liver disease is one of the major causes of morbidity and mortality across the world. According to WHO estimates, about 500 million people are living with chronic hepatitis infections resulting in the death of over one million people annually. Medicinal plants serve as a vital source of potentially useful new compounds for the development of effective therapy to combat liver problems. Moreover herbal products have the advantage of better affordability and acceptability, better compatibility with the human body, and minimal side effects and is easier to store. In this review attempt has been made to summarize the scientific data published on hepatoprotective plants used in Saudi Arabian traditional medicine. The information includes medicinal uses of the plants, distribution in Saudi Arabia, ethnopharmacological profile, possible mechanism of action, chemical constituents, and toxicity data. Comprehensive scientific studies on safety and efficacy of these plants can revitalise the treatment of liver diseases.
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15
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Girdhar M, Sharma NR, Rehman H, Kumar A, Mohan A. Comparative assessment for hyperaccumulatory and phytoremediation capability of three wild weeds. 3 Biotech 2014; 4:579-589. [PMID: 28324308 PMCID: PMC4235884 DOI: 10.1007/s13205-014-0194-0] [Citation(s) in RCA: 22] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/21/2013] [Accepted: 01/03/2014] [Indexed: 11/10/2022] Open
Abstract
The composition and the organization of soil are changing rapidly by the diverged mankind activities, leading to the contamination of environment. Several methods are employed to clean up the environment from these kinds of contaminants, but most of them are costly and ineffective to yield optimum results. Phytoremediation is a natural green technology, which is eco-friendly for the removal of toxic metals from the polluted environment. Phytoremediation is a cost-effective technique through which the cleanup of contaminated soil laced with heavy metals is performed by wild weeds and small herbal plants. The phytoremediation technique provides a promising tool for hyperaccumulation of heavy metals; arsenic, lead, mercury, copper, chromium, and nickel, etc., by the wild weeds and that has been discussed here in detail in case of Cannabissativa, Solanum nigrum and Rorippa globosa. In general, weeds that have the intrinsic capacity to accumulate metals into their shoots and roots, have the ability to form phytochelates and formation of stable compound with ions. This behavior of accumulation along with chelate and stable compound formation is utilized as a tool for phytoremediation activity.
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Affiliation(s)
- Madhuri Girdhar
- Department of Biotechnology, Lovely Professional University, Chehru, Phagwara, India
| | - Neeta Raj Sharma
- Department of Biotechnology, Lovely Professional University, Chehru, Phagwara, India
| | - Hasibur Rehman
- Department of Biology, Faculty of Sciences, University of Tabuk, Tabuk, Saudi Arabia
| | - Anupam Kumar
- Department of Biotechnology, Lovely Professional University, Chehru, Phagwara, India
| | - Anand Mohan
- Department of Biotechnology, Lovely Professional University, Chehru, Phagwara, India.
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LI GUIJIE, SUN PENG, WANG QIANG, QIAN YU, ZHU KAI, ZHAO XIN. Dendrobium candidum Wall. ex Lindl. attenuates CCl 4-induced hepatic damage in imprinting control region mice. Exp Ther Med 2014; 8:1015-1021. [PMID: 25120640 PMCID: PMC4113641 DOI: 10.3892/etm.2014.1834] [Citation(s) in RCA: 14] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/08/2014] [Accepted: 06/18/2014] [Indexed: 01/13/2023] Open
Abstract
The aim of the present study was to determine the preventive effect of the traditional Chinese medicine, Dendrobium candidum Wall ex Lindl. (D. candidum), on CCl4-induced hepatic damage in mice. The CCl4-induced hepatic damage mice were treated with D. candidum, and the serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), triglyceride (TG) and total cholesterol (TC) were determined. In addition, serum cytokine levels of interleukin (IL)-6, IL-12, tumor necrosis factor (TNF)-α and interferon (IFN)-γ were analyzed with kits, while liver tissues were analyzed using hematoxylin and eosin staining and reverse transcription polymerase chain reaction (RT-PCR). Furthermore, the contents of D. candidum were determined by nuclear magnetic resonance (NMR). D. candidum was demonstrated to successfully prevent hepatic damage in mice. The serum levels of AST, ALT and LDH were significantly decreased when the mice were treated with 200 and 400 mg/kg D. candidum, as compared with the control mice (P<0.05). The lowest enzymatic activities were exhibited in the 400 mg/kg D. candidum group, which produced similar results to the positive control drug, silymarin. In addition, in the 400 mg/kg D. candidum group, the highest levels of TG and TC were observed among the treated groups. D. candidum-treated groups also demonstrated reduced levels of the serum proinflammatory cytokines, IL-6, IL-12, TNF-α and IFN-γ. The sections of liver tissue examined during histopathology in the high concentration 400 mg/kg D. candidum group recovered well from CCl4 damage; however, the sections in the 200 mg/kg D. candidum group revealed necrosis to a more serious degree. RT-PCR analysis was conducted on inflammation-associated genes, including nuclear factor (NF)-κB, IκB-α, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, in the livers of the mice. The 400 mg/kg D. candidum group demonstrated significantly decreased mRNA expression levels of NF-κB, iNOS and COX-2, but an increased expression level of IκB-α when compared with the CCl4-treated control group. Furthermore, using NMR, 11 compounds were identified in the D. candidum leaf, whose functional contents may aid the preventive effect observed in the current study. Therefore, D. candidum may potentially contribute to the prevention of CCl4-induced hepatic damage in vivo.
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Affiliation(s)
| | | | - QIANG WANG
- Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China
| | - YU QIAN
- Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China
| | - KAI ZHU
- Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China
| | - XIN ZHAO
- Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China
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Abdel-Rahim EA, Abdel-Mobdy YE, Ali RF, Mahmoud HA. Hepatoprotective effects of Solanum nigrum Linn fruits against cadmium chloride toxicity in albino rats. Biol Trace Elem Res 2014; 160:400-8. [PMID: 25022247 DOI: 10.1007/s12011-014-9994-7] [Citation(s) in RCA: 8] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 02/12/2014] [Accepted: 04/23/2014] [Indexed: 10/25/2022]
Abstract
The present work is aimed to investigate the toxicity of 1/20 LD50 of cadmium chloride (CdCl2) on male albino rats by oral ingestion and to determine the hepatoprotective effect of Solanum nigrum Linn (SN) dried fruits and their ethanolic extract against CdCl2 toxicity using biochemical parameters. Rats were divided into six groups; the first group is control, second group is CdCl2-intoxicated rats, third group is fed with a semi-modified diet with S. nigrum fruits, fourth group rats ingested with dried extract, and intoxicated rats (groups 5 and 6) were treated with fruits and ethanolic extract of S. nigrum, respectively. The results showed that rats exposed to CdCl2 induced remarkable decrease in body weight gain, feed efficiency, and Hb, Hct, RBC, and WBC count and MCHC, but increase in MCV and MCH values. In the case of plasma enzymes, there were significant stimulations observed in ALT and AST, acid phosphatase, alkaline phosphatase, and LDH activities of CdCl2-intoxicated rats (group 2) compared to control (group 1). Plasma protein profile showed decreases in total soluble protein and albumin; also globulin content was decreased by CdCl2 ingestion. Under the same condition, plasma total bilirubin and glucose levels were increased in group 2. In addition, lipid peroxidation and antioxidative system (GSH, catalase, and SOD) of liver were harmed by CdCl2 ingestion. Whereas, normal rats treated with SN showed insignificant changes in groups 3 and 4 as compared to control (group 1). The treatment with dried fruits and their ethanolic extract in CdCl2-intoxicated rats (groups 5 and 6) ameliorated and improved these harmful effects in all above parameters either for blood or liver. The results of this study suggest the protective effect of S. nigrum against liver injury happened by CdCl2 which may be attributed to its hepatoprotective activity and thereby.
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Affiliation(s)
- Emam A Abdel-Rahim
- Department of Biochemistry, Faculty of Agriculture, Cairo University, Cairo, Egypt,
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18
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H.O. Mogla E, M. Abdalla O, Koko W, Saadabi A. In vitro Anticancer Activity and Cytotoxicity of Solanum nigrum on Cancers and Normal Cell Lines. ACTA ACUST UNITED AC 2014. [DOI: 10.3923/ijcr.2014.74.80] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/15/2022]
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El-Meligy RM, Awaad AS, Soliman GA, Bacha AB, Alafeefy AM, Kenawy SA. Prophylactic and curative anti-ulcerative colitis activity and the possible mechanisms of action of some desert plants. J Enzyme Inhib Med Chem 2014; 30:250-8. [PMID: 24811429 DOI: 10.3109/14756366.2014.915394] [Citation(s) in RCA: 14] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/07/2023] Open
Abstract
The aim of the present study was to evaluate both prophylactic and curative anti-ulcerative colitis activity and the possible mechanism of action of seven desert plant extracts. Seven desert plants from different families; Conyza dioscoridis (L.) Desf. (Asteraceae), Euphorbia hirta L. (Euphorpiaceae), Origanum syriacum L. and Salvia lanigera L. (Lamiaceae), Sisymbrium irio L., Solanum nigrum Linn. (Solanaceae) and Solenostemma arghel (Del.) Hayne. (Asclepiadaceae) were separately evaluated at three doses (125, 250, and 500 mg/kg) using the acetic acid-induced colitis model. The investigated extracts possessed prophylactic and curative anti-ulcerative colitis activities in a dose-dependent manner, where Salvia lanigera (87.9) and Solenostemma arghel (89.2) were the most effective extracts whereas the dexamesathone produced 68%. These extracts were further investigated for estimation of their mechanism of action. The in vitro potential radical (DPPH) scavenging activities of the investigated extracts were well supported with the reduction of colonic MDA content for both extracts. Suppression of the inflammatory mediator TNF-α and inhibition of both PLA2 and protease enzymes may play an important role in the anti-ulcerative colitis activities. The investigated extracts were safe for use up to 5 g/kg and the total alcohol extracts of Salvia lanigera and Solenostemma arghel (400 mg/kg for 35 d) showed no alteration on liver and kidney functions. Phytochemical screening of the investigated extracts revealed the presence of flavonoids, tannins, unsaturated sterols, and proteins which could be responsible for the activities.
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Affiliation(s)
- Reham M El-Meligy
- Chemistry Department, Faculty of Science, King Saud University , Riyadh , KSA
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20
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Biosynthesis of gold nanoparticles using Solanum nigrum leaf extract and screening their free radical scavenging and antibacterial properties. ACTA ACUST UNITED AC 2014. [DOI: 10.1016/j.bionut.2014.03.004] [Citation(s) in RCA: 105] [Impact Index Per Article: 9.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/18/2022]
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Pestana M, Rodrigues M, Teixeira L, Abrantes IDO, Gouveia M, Cordeiro N. In vitro evaluation of nematicidal properties of Solanum sisymbriifolium and S. nigrum extracts on Pratylenchus goodeyi. NEMATOLOGY 2014. [DOI: 10.1163/15685411-00002743] [Citation(s) in RCA: 7] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/19/2022]
Abstract
The root-lesion nematode, Pratylenchus goodeyi, is among the most economically damaging parasites of banana plants. Nematode control can benefit from searching for novel bio-nematicides. The present study was carried out to assess the potential nematicidal properties of two Solanum species (Solanum sisymbriifolium and S. nigrum) against P. goodeyi, using dichloromethane, acetone, ethanol and either cold or hot water extracts of the plants. Water extracts of both plants at a concentration of 10 mg ml−1 greatly affected nematode movement and also caused mortality. The analysis of sequential extracts at the same experimental concentrations showed that, although water extracts affect nematode mobility and mortality, the acetone extract from S. nigrum was the most efficient, causing 100% mortality after 23 h exposure. The results showed that S. sisymbriifolium and S. nigrum extracts contain chemical components that induce morphological changes in the body structure of the root-lesion nematode, affect mobility and cause mortality. The nematostatic and nematicidal potential of the extracts described herein merit further studies to find novel bio-nematicides against the root-lesion nematode.
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Affiliation(s)
- Margarida Pestana
- 1Madeira Agricultural Laboratory, RAM, 9135-372 Santa Cruz, Portugal
- 2Centre of Life Sciences, University of Madeira, 9000-390 Funchal, Portugal
| | - Mónica Rodrigues
- 2Centre of Life Sciences, University of Madeira, 9000-390 Funchal, Portugal
| | - Lucília Teixeira
- 2Centre of Life Sciences, University of Madeira, 9000-390 Funchal, Portugal
| | - Isabel de O. Abrantes
- 3IMAR-CMA, Department of Life Sciences, University of Coimbra, 3004-517 Coimbra, Portugal
| | - Manuela Gouveia
- 2Centre of Life Sciences, University of Madeira, 9000-390 Funchal, Portugal
| | - Nereida Cordeiro
- 4Centre of Exact Science and Engineering, University of Madeira, 9000-390 Funchal, Portugal
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Zhao X, Song JL, Kil JH, Park KY. Bamboo salt attenuates CCl4-induced hepatic damage in Sprague-Dawley rats. Nutr Res Pract 2013; 7:273-80. [PMID: 23964314 PMCID: PMC3746161 DOI: 10.4162/nrp.2013.7.4.273] [Citation(s) in RCA: 14] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/24/2013] [Revised: 04/11/2013] [Accepted: 04/16/2013] [Indexed: 01/17/2023] Open
Abstract
Bamboo salt, a Korean folk medicine, is prepared with solar salt (sea salt) and baked several times at high temperatures in a bamboo case. In this study, we compared the preventive effects of bamboo salt and purified and solar salts on hepatic damage induced by carbon tetrachloride in Sprague-Dawley rats. Compared with purified and solar salts, bamboo salts prevented hepatic damage in rats, as evidenced by significantly reduced serum levels of aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase (P < 0.05). Bamboo salt (baked 9×) triggered the greatest reduction in these enzyme levels. In addition, it also reduced the levels of the proinflammatory cytokines interleukin (IL)-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α. Histopathological sections of liver tissue demonstrated the protective effect of bamboo salt, whereas sections from animals treated with the other salt groups showed a greater degree of necrosis. We also performed reverse transcription-polymerase chain reaction and western blot analyses of the inflammation-related genes iNOS, COX-2, TNF-α, and IL-1β in rat liver tissues. Bamboo salt induced a significant decrease (~80%) in mRNA and protein expression levels of COX-2, iNOS, TNF-α, and IL-1β, compared with the other salts. Thus, we found that baked bamboo salt preparations could prevent CCl4-induced hepatic damage in vivo.
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Affiliation(s)
- Xin Zhao
- Department of Food Science and Nutrition, Pusan National University, 30 Jangjun-dong, Geumjung-gu, Busan 609-735, Korea. ; Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, PR China
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23
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Dhiman A, Nanda A, Ahmad S. A recent update in research on the antihepatotoxic potential of medicinal plants. ACTA ACUST UNITED AC 2012; 10:117-27. [PMID: 22313878 DOI: 10.3736/jcim20120201] [Citation(s) in RCA: 30] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/11/2022]
Abstract
Hepatic damage is a global metabolic and epidemic disease, affecting essential biochemical activities in almost every age group. Conventional drugs used in the treatment of liver disorders are often inadequate. Also, the spectrum of liver abnormalities caused by allopathic drugs is found to be broad. In view of severely undesirable side effects of synthetic agents, it is necessary to search for alternative drugs for the treatment of liver diseases to replace the currently used drugs, which are of doubtful efficacy and safety. Therefore, there is growing focus to follow systematic research methodology and to evaluate scientific basis for the traditional herbal medicines that are claimed to possess hepatoprotective activities. Use of herbal drugs in the treatment of liver diseases has a long tradition, especially in Eastern medicine and can be traced back as far as 2100 B.C. in ancient China (Xia Dynasty) and India (Vedic period), but evidence for efficacy is sparse. The current study is aimed at providing an overview of clinical and experimental studies carried out on the most effective and commonly used hepatoprotective plants and their beneficial aspects.
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Affiliation(s)
- Anju Dhiman
- Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak 124001, Haryana, India.
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24
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Zhao X. Hawk tea (Litsea coreana Levl. var. lanuginose) attenuates CCl(4)-induced hepatic damage in Sprague-Dawley rats. Exp Ther Med 2012; 5:555-560. [PMID: 23403509 PMCID: PMC3570157 DOI: 10.3892/etm.2012.840] [Citation(s) in RCA: 22] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/27/2012] [Accepted: 11/26/2012] [Indexed: 01/17/2023] Open
Abstract
Hawk tea (Litsea coreana Levl. var. lanuginose) is a traditional Chinese drink similar to green tea. In the present study, the preventive effects of Hawk tea on hepatic damage induced by carbon tetrachloride (CCl4) were studied in Sprague-Dawley rats. Silymarin was used as a positive control. Hawk tea was successfully shown to prevent hepatic damage in the rats. Serum levels of AST, ALT and LDH were significantly decreased when the rats were treated with varying concentrations of Hawk tea compared with silymarin (P<0.05). The lowest enzyme activities were exhibited in the 400 mg/kg Hawk tea group. This group showed reduced levels of the serum proinflammatory cytokines IL-6, IFN-γ and TNF-α. In particular, the IFN-γ level decreased markedly compared with the other concentration groups. The histopathology sections of liver tissue in the 400 mg/kg Hawk tea group recovered well from the CCl4 damage, but the sections of the other concentration groups showed necrosis to a more serious degree. Reverse transcription-polymerase chain reaction (RT-PCR) and western blot analyses of the inflammation-related genes iNOS, COX-2, TNF-α and IL-1β in the rat livers were tested. The 400 mg/kg Hawk tea group showed significantly decreased mRNA and protein expression levels of iNOS, COX-2, TNF-α and IL-1β compared with the control group. Accordingly, 400 mg/kg Hawk tea potentially contributes to the prevention of CCl4-induced hepatic damage in vivo. A 200 or 100 mg/kg dose of Hawk tea also demonstrated preventive effects against hepatic damage.
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Affiliation(s)
- Xin Zhao
- Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China
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Kang H, Jeong HD, Choi HY. The Chloroform Fraction of Solanum nigrum Suppresses Nitric Oxide and Tumor Necrosis Factor-α in LPS-Stimulated Mouse Peritoneal Macrophages Through Inhibition of p38, JNK and ERK1/2. THE AMERICAN JOURNAL OF CHINESE MEDICINE 2012; 39:1261-73. [DOI: 10.1142/s0192415x11009548] [Citation(s) in RCA: 14] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/14/2022]
Abstract
Solanum nigrum L., commonly known as black nightshade, is used worldwide for the treatment of skin and mucosal ulcers, liver cirrhosis and edema. We aimed to determine the anti-inflammatory active fraction of S. nigrum by serial extractions. S. nigrum was first extracted with methanol, then fractionated with chloroform and water. The effects of S. nigrum fractions, diosgenin and α-solanine on LPS/interferon-gamma-induced nitric oxide (NO) and inducible NO synthase (iNOS), or LPS-induced tumor necrosis factor-α (TNF-α) and interleukin (IL)-6, in mouse peritoneal macrophages were determined. Western blotting analysis was used to detect LPS-induced phosphorylation of p38, JNK and ERK1/2. The chloroform fraction of S. nigrum was cytotoxic in a time and concentration dependent manner; however, the methanol and water fractions were not. The chloroform fraction reduced NO through inhibition of iNOS synthesis and inhibited TNF-α and IL-6 at the level of protein secretion; the methanol and water fractions showed a weak or no effect. The chloroform fraction also suppressed p38, JNK and ERK1/2. Diosgenin and α-solanine were cytotoxic at a high concentration. In particular, diosgenin was able to inhibit TNF-α and IL-6, but both compounds did not affect LPS-induced iNOS expression. These results indicate that the anti-inflammatory compounds of S. nigrum exist preferentially in the nonpolar fraction, ruling out the possibility that diosgenin and α-solanine are the likely candidates. The inhibition of iNOS, TNF-α and IL-6 by the chloroform fraction may be partly due to the suppression of p38, JNK and ERK1/2. Further study is required to identify the active compounds of S. nigrum.
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Affiliation(s)
- Hee Kang
- Department of East-West Medical Science, Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Gyeonggi-do 446-701, South Korea
| | - Ha-Deok Jeong
- Department of Herbology, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, South Korea
| | - Ho-Young Choi
- Department of Herbology, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, South Korea
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NAWAB AKBAR, THAKUR VIJAYS, YUNUS MOHAMMAD, MAHDI ABBASALI, GUPTA SANJAY. Selective cell cycle arrest and induction of apoptosis in human prostate cancer cells by a polyphenol-rich extract of Solanum nigrum. Int J Mol Med 2012; 29:277-84. [PMID: 22076244 PMCID: PMC3981642 DOI: 10.3892/ijmm.2011.835] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/19/2011] [Accepted: 09/02/2011] [Indexed: 01/05/2023] Open
Abstract
Progression of prostate cancer is associated with escape of tumor cells from cell cycle arrest and apoptosis. Agents capable of selectively eliminating cancer cells by cell cycle arrest and/or induction of apoptosis offer a highly desirable approach. Here we demonstrate that a polyphenolic extract derived from ripe berries of Solanum nigrum (SN) differentially causes cell cycle arrest and apoptosis in various human prostate cancer cells without affecting normal prostate epithelial cells. Virally transformed normal human prostate epithelial PZ-HPV-7 cells and their cancer counterpart CA-HPV-10 cells, were used to evaluate the growth-inhibitory effects of the SN extract. SN treatment (5-20 µg/ml) of PZ-HPV-7 cells resulted in growth inhibitory responses of low magnitude. In sharp contrast, SN treatment of CA-HPV-10 cells increased cytotoxicity, decreased cell viability and induced apoptosis. Similar results were noted in the human prostate cancer LNCaP, 22Rv1, DU145 and PC-3 cell lines, where significant reductions in cell viability and induction of apoptosis was observed in all these cells, an effect independent of disease stage and androgen association. Cell cycle analysis revealed that SN treatment (5-20 µg/ml) resulted in a dose-dependent G2/M phase arrest and subG1 accumulation in the CA-HPV-10 but not in the PZ-HPV-7 cell line. Our results, for the first time, demonstrate that the SN extract is capable of selectively inhibiting cellular proliferation and accelerating apoptotic events in prostate cancer cells. SN may be developed as a promising therapeutic and/or preventive agent against prostate cancer.
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Affiliation(s)
- AKBAR NAWAB
- Department of Urology, Case Western Reserve University, Cleveland, OH 44106, USA
- The Urology Institute, University Hospitals Case Medical Center, Cleveland, OH 44106, USA
- Department of Environmental Sciences, Babasaheb Bhimrao Ambedkar University, Lucknow 226025, India
| | - VIJAY S. THAKUR
- Department of Urology, Case Western Reserve University, Cleveland, OH 44106, USA
- The Urology Institute, University Hospitals Case Medical Center, Cleveland, OH 44106, USA
| | - MOHAMMAD YUNUS
- Department of Environmental Sciences, Babasaheb Bhimrao Ambedkar University, Lucknow 226025, India
| | - ABBAS ALI MAHDI
- Department of Biochemistry, King George’s Medical University, Lucknow 226003, India
| | - SANJAY GUPTA
- Department of Urology, Case Western Reserve University, Cleveland, OH 44106, USA
- The Urology Institute, University Hospitals Case Medical Center, Cleveland, OH 44106, USA
- Case Comprehensive Cancer Center, Cleveland, OH 44106, USA
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Singhal VK, Kaur M, Kumar HP, Gupta RC. High Pollen Sterility and 2 n Pollen Grains in an Asynaptic 4 x Cytotype (2 n=48) of Solanum nigrum L. CYTOLOGIA 2012. [DOI: 10.1508/cytologia.77.333] [Citation(s) in RCA: 12] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/16/2022]
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Wang L, Gao G, Bai Y, Luo W, Lin C, Jia Q. Fingerprint quality detection of Solanum nigrum using high-performance liquid chromatography-evaporative light scattering detection. PHARMACEUTICAL BIOLOGY 2011; 49:595-601. [PMID: 21554001 DOI: 10.3109/13880209.2010.535171] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/30/2023]
Abstract
CONTEXT Solanum nigrum Linn. (Solanaceae), a traditional Chinese medicine (TCM), has been used for cancer therapy. It is urgent to develop a novel quality standard to validly detect its quality. OBJECTIVE To control its quality, a novel, accurate, and valid fingerprint method was developed by high-performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD) in the current case. We could evaluate the quality of different batches and assure the stability of herbs' quality in subsequent research. MATERIALS AND METHODS The HPLC-ELSD fingerprints have been developed through analyzing 41 batches of raw herbs collected from different areas in different harvesting time. RESULTS We have determined the optimum extraction and detection conditions in the process of establishing herb fingerprint. And, we could establish reference fingerprint to control such herb quality. Also, we could determine optimum collecting location and harvesting time according to the fingerprint. DISCUSSION AND CONCLUSION It is the first time a new method has been established to control the quality of S. nigrum through HPLC-ELSD. We developed combining similarity evaluation to identify and distinguish raw materials efficiently from different sources. For S. nigrum the most influenced factor on herb quality was the collecting location, and the next was the harvesting time. So, in order to get the consistent raw materials, the collecting location and the harvesting time should be fixed.
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Affiliation(s)
- Liye Wang
- Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, P. R. China
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29
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Loganayaki N, Siddhuraju P, Manian S. Antioxidant activity of two traditional Indian vegetables: Solanum nigrum L. and Solanum torvum L. Food Sci Biotechnol 2010. [DOI: 10.1007/s10068-010-0017-y] [Citation(s) in RCA: 14] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/19/2022] Open
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Arulmozhi V, Krishnaveni M, Karthishwaran K, Dhamodharan G, Mirunalini S. Antioxidant and antihyperlipidemic effect of Solanum nigrum fruit extract on the experimental model against chronic ethanol toxicity. Pharmacogn Mag 2010; 6:42-50. [PMID: 20548935 PMCID: PMC2881655 DOI: 10.4103/0973-1296.59965] [Citation(s) in RCA: 36] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/29/2009] [Revised: 12/07/2009] [Accepted: 02/13/2010] [Indexed: 11/20/2022] Open
Abstract
The possible protective effect of Solanum nigrum fruit extract (SNFEt) was investigated for its antioxidant and antihyperlipidemic activity against ethanol-induced toxicity in rats. The experimental animals were intoxicated with 20% ethanol (7.9 g/kg/day) for 30 days via gastric intubation. SNFEt was administered at the dose of 250 mg/kg body weight along with the daily dose of ethanol for 30 days. From the result it was observed that ethanol-induced rats showed a significant elevation in the levels of Thiobarbituric acid reactive substances (TBARS), which lowered the antioxidant defense systems, such as, reduced glutathione (GSH) and vitamins C and E, when compared to the controls. In the lipid profiles, the levels of total cholesterol (TC), triglycerides (TG), low density lipoproteins (LDL), very low density lipoproteins (VLDL), free fatty acids (FFA), and phospholipids were significantly elevated in the ethanol-induced group, whereas, the high density lipoproteins (HDL) were found to be reduced in the plasma, and the phospholipid levels were significantly decreased in the tissues. Supplementation of SNFEt improved the antioxidant status by decreasing the levels of TBARS and altering the lipid profiles to near normal. These activities were also compared to the standard drug silymarin (25 mg/kg body weight). Thus the findings of the present study indicated a significant antioxidant and antihyperlipidemic activity of Solanum nigrum fruits, which offered protection against ethanol-induced toxicity.
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Affiliation(s)
- Vadivel Arulmozhi
- Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar - 608 002, Tamil Nadu, India
| | - Mani Krishnaveni
- Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar - 608 002, Tamil Nadu, India
| | - Kandhan Karthishwaran
- Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar - 608 002, Tamil Nadu, India
| | - Ganesan Dhamodharan
- Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar - 608 002, Tamil Nadu, India
| | - Sankaran Mirunalini
- Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar - 608 002, Tamil Nadu, India
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Chandrasekaran CV, Sundarajan K, David K, Agarwal A. In vitro efficacy and safety of poly-herbal formulations. Toxicol In Vitro 2009; 24:885-97. [PMID: 19958825 DOI: 10.1016/j.tiv.2009.11.021] [Citation(s) in RCA: 16] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/20/2009] [Revised: 10/23/2009] [Accepted: 11/26/2009] [Indexed: 11/26/2022]
Abstract
Indigenous plants are used as a traditional source of raw materials for the manufacture of medicines. Modernizing the ancient art of herbal medicine bequeathed from generations entails addressing two interrelated issues i.e. efficacy, and safety prior to their acceptance and use worldwide. The present study was designed to investigate three of our veterinary poly-herbal formulations - Phytocee an antistressor; Zigbir(R) a hepatoprotectant; and Zist(R) as an immunomodulator in the pertinent in vitro cell assay models in order to validate their therapeutic potential. Cellular antioxidant potential of Phytocee was demonstrated against AAPH induced oxidative stress using HepG2 cells. Zigbir(R) was confirmed as a hepatoprotectant against tert-butylhydroperoxide induced cytotoxicity in HepG2 cells. Immunomodulatory activity of Zist(R) was established by its ability to inhibit the proliferation of mitogen stimulated murine splenocytes in vitro. On treatment with Zist(R), a trend of decline in IL-6, and IL-12 levels was observed following stimulation with Con A, and LPS respectively in murine splenocytes. Further, all the three poly-herbal formulations were subjected to Ames II assay for ensuring their safety profile. Results epitomize that all the three poly-herbal formulations were devoid of significant mutagenic effect in TA98, and TAMix strains of Salmonella typhimurium under our experimental conditions.
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Affiliation(s)
- C V Chandrasekaran
- Department of Cellular Assay, R&D Centre, Natural Remedies Pvt Ltd, Bangalore 560 100, Karnataka, India.
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Mukherjee PK, Sahoo AK, Narayanan N, Kumar NS, Ponnusankar S. Lead finding from medicinal plants with hepatoprotective potentials. Expert Opin Drug Discov 2009; 4:545-76. [DOI: 10.1517/17460440902911433] [Citation(s) in RCA: 24] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/12/2023]
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Antihepatopathic Plants Used by the Lepcha Tribe of the Sikkim and Darjeeling Himalayan Region of India. ACTA ACUST UNITED AC 2008. [DOI: 10.1300/j044v13n03_03] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/18/2022]
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Hsieh CC, Fang HL, Lina WC. Inhibitory effect of Solanum nigrum on thioacetamide-induced liver fibrosis in mice. JOURNAL OF ETHNOPHARMACOLOGY 2008; 119:117-121. [PMID: 18606216 DOI: 10.1016/j.jep.2008.06.002] [Citation(s) in RCA: 31] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/06/2007] [Revised: 05/25/2008] [Accepted: 06/07/2008] [Indexed: 05/26/2023]
Abstract
AIM Solanum nigrum (Solanaceae) has been used in traditional folk medicine for its hepatoprotective agent. The purpose of this study was to investigate the effects of Solanum nigrum extract (SNE) on thioacetamide (TAA)-induced liver fibrosis in mice. MATERIALS AND METHODS Hepatic fibrosis was produced by TAA (0.2 g/kg, i.p.) three times a week for 12 weeks. Mice in the three TAA groups were treated daily with distilled water and SNE (0.2 or 1.0 g/kg) via gastrogavage throughout the experimental period. RESULTS SNE reduced the hepatic hydroxyproline and alpha-smooth muscle actin protein levels of TAA-treated mice. SNE inhibited TAA-induced collagene (alpha1)(I) and transforming growth factor-beta1 (TGF-beta1) mRNA levels in the liver. Histological examination also confirmed that SNE reduced the degree of fibrosis caused by TAA treatment. CONCLUSION Oral administration of SNE significantly reduces TAA-induced hepatic fibrosis in mice, probably through the reduction of TGF-beta1 secretion.
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Affiliation(s)
- Chang-Chi Hsieh
- Department of Animal Science and Biotechnology, Tunghai University, Taichung, Taiwan
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Lin HM, Tseng HC, Wang CJ, Chyau CC, Liao KK, Peng PL, Chou FP. Induction of autophagy and apoptosis by the extract of Solanum nigrum Linn in HepG2 cells. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2007; 55:3620-8. [PMID: 17419635 DOI: 10.1021/jf062406m] [Citation(s) in RCA: 54] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/14/2023]
Abstract
Solanum nigrum L. (SN) has been used in traditional folk medicine to treat different cancers. It is also used as a hepatoprotective and anti-inflammatory agent. In this study, we demonstrated that the extract of SN (SNE) induced a strong cytotoxic effect toward HepG2 cells but much less to Chang liver and WRL-68 cells. The mechanisms of the cytotoxic effect were concentration-dependent. High doses of SNE (2 and 5 mg/mL) induced apoptotic cell death in HepG2 cells, as evidenced by increases in the expressions of p-JNK and Bax, mitochodrial release of cytochrome c, and caspase activation. On the other hand, cells treated with low concentrations of SNE (50-1000 microg/mL) revealed morphological and ultrastructural changes of autophagocytic death under electron microscopic observation. Furthermore, these cells showed increased levels of autophagic vacuoles and LC3-I and LC3-II proteins, specific markers of autophagy. The levels of Bcl-2 and Akt that have been implicated in the down-regulation of autophagy were decreased upon SNE treatment. Taken together, these findings indicate that SNE induced cell death in hepatoma cells via two distinct antineoplastic activities of SNE, the ability to induce apoptosis and autophagocytosis, therefore suggesting that it may provide leverage to treat liver cancer.
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Affiliation(s)
- Hui-Mei Lin
- Institute of Biochemistry and Biotechnology, College of Medicine, Chung Shan Medical University, Taichung, Taiwan
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Zakaria ZA, Gopalan HK, Zainal H, Mohd Pojan NH, Morsid NA, Aris A, Sulaiman MR. Antinociceptive, Anti-inflammatory and Antipyretic Effects of Solanum nigrum Chloroform Extract in Animal Models. YAKUGAKU ZASSHI 2006; 126:1171-8. [PMID: 17077618 DOI: 10.1248/yakushi.126.1171] [Citation(s) in RCA: 43] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/22/2022]
Abstract
AIM The present study was carried out to evaluate the antinociceptive, anti-inflammatory and antipyretic effects of chloroform extract of Solanum nigrum leaves using various animal models. METHODS The extract was prepared by soaking (1:20; w/v) the air-dried powdered leaves (20 g) in chloroform for 72 hrs followed by evaporation (40 degrees C) under reduced pressure to dryness (1.26 g) and then dissolved (1:50; w/v) in dimethylsulfoxide (DMSO). The supernatant, considered as the stock solution with dose of 200 mg/kg, was diluted using DMSO to 20 and 100 mg/kg, and all doses were administered (s.c.; 10 ml/kg) in mice/rats 30 min prior to tests. RESULTS The extract exhibited significant (p<0.05) antinociceptive activity when assessed using the abdominal constriction, hot plate and formalin tests. The extract also produced significant (p<0.05) anti-inflammatory and antipyretic activities when assessed using the carrageenan-induced paw edema and brewer's yeast-induced pyrexia tests. Overall, the activities occurred in a dose-independent manner. CONCLUSION The present study demonstrated that the lipid-soluble extract of S. nigrum leaves possessed antinociceptive, anti-inflammatory and anti-pyretic properties and confirmed the traditional claims.
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Affiliation(s)
- Zainul Amiruddin Zakaria
- Faculty of Biotechnology and Life Sciences, Universiti Industri Selangor, Jalan Zirkon A7/A, Seksyen 7, 40000 Shah Alam, Selangor, Malaysia.
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Jainu M, Devi CSS. Antiulcerogenic and ulcer healing effects of Solanum nigrum (L.) on experimental ulcer models: possible mechanism for the inhibition of acid formation. JOURNAL OF ETHNOPHARMACOLOGY 2006; 104:156-63. [PMID: 16202548 DOI: 10.1016/j.jep.2005.08.064] [Citation(s) in RCA: 74] [Impact Index Per Article: 3.9] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/17/2005] [Revised: 07/10/2005] [Accepted: 08/27/2005] [Indexed: 05/04/2023]
Abstract
Solanum nigrum, an herbal plant which is recommended in ayurveda for the management of gastric ulcers. Therefore, the purpose of the study was to investigate the antiulcer effect of Solanum nigrum fruits extract (SNE) on cold restraint stress (CRU), indomethacin (IND), pyloric ligation (PL) and ethanol (EtOH) induced gastric ulcer models and ulcer healing activity on acetic acid induced ulcer model in rats. The treatment with SNE at higher dose significantly inhibited the gastric lesions induced by CRU (76.6%), IND (73.8%), PL (80.1%) and EtOH (70.6%), respectively, with equal or higher potency than omeprazole. SNE showed concomitant attenuation of gastric secretory volume, acidity and pepsin secretion in ulcerated rats. In addition, SNE (200 and 400mg/kgb.w.) accelerated the healing of acetic acid induced ulcers after the treatment for 7 days. Further, to ascertain the antisecretory action, the effects of SNE on H(+)K(+)ATPase activity and plasma concentration of gastrin hormone in ulcerated rats were determined. SNE significantly inhibits H(+)K(+)ATPase activity and decreases the gastrin secretion in EtOH-induced ulcer model. The severity of the reaction of ulcerogen and the reduction of ulcer size by SNE was evident by histological findings. Toxicity studies of SNE have also been carried out for its safety evaluation. SNE, thus, offers antiulcer activity by blocking acid secretion through inhibition of H(+)K(+)ATPase and decrease of gastrin secretion. These results further suggest that SNE was found to possess antiulcerogenic as well as ulcer healing properties, which might also be due to its antisecretory activity.
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Affiliation(s)
- Mallika Jainu
- Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India.
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Effects of Callophyllis japonica powder on carbon tetrachloride-induced liver injury in rats. ACTA ACUST UNITED AC 2005. [DOI: 10.3742/opem.2005.5.3.231] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/07/2022]
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An HJ, Kwon KB, Cho HI, Seo EA, Ryu DG, Hwang WJ, Yoo SJ, Kim YK, Hong SH, Kim HM. Solanum nigrum produces nitric oxide via nuclear factor-kappaB activation in mouse peritoneal macrophages. Eur J Cancer Prev 2005; 14:345-50. [PMID: 16030424 DOI: 10.1097/00008469-200508000-00006] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/25/2022]
Abstract
Nitric oxide (NO) is an antitumour molecule produced in activated macrophages and Solanum nigrum is a plant used in oriental medicine to treat tumours. In this study using mouse peritoneal macrophages, we have examined the mechanism by which Solanum nigrum regulates NO production. When Solanum nigrum was used in combination with 20 U/ml of recombinant interferon-gamma (rIFN-gamma), there was a marked cooperative induction of NO production. The increase in NO synthesis was reflected as an increased amount of inducible NO synthase (iNOS) protein. The production of NO from rIFN-gamma plus Solanum nigrum-stimulated peritoneal macrophages was decreased by treatment with N-monomethyl-L-arginine or N-tosyl-Phe chloromethyl ketone, an iNOS inhibitor. Additionally, the increased production of NO from rIFN-gamma plus Solanum nigrum-stimulated cells was almost completely inhibited by pretreatment with 100 micromol/l of pyrrolidine dithiocarbamate, an inhibitor of nuclear factor kappaB (NF-kappaB). Furthermore, Solanum nigrum increased activation of NF-kappaB. These findings suggest that Solanum nigrum increases the production of NO by rIFN-gamma-primed macrophages and NF-kappaB plays a critical role in mediating these effects.
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Affiliation(s)
- H-J An
- College of Oriental Medicine, Kyung Hee University, Seoul, 130-701, South Korea
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Huang FJ, Lv ZB, Li Q, Wei LJ, Zhang L, Wu WT. Study on hepatoprotective effect of peptide S-8300 from shark liver. World J Gastroenterol 2005; 11:1809-12. [PMID: 15793870 PMCID: PMC4305880 DOI: 10.3748/wjg.v11.i12.1809] [Citation(s) in RCA: 7] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 02/06/2023] Open
Abstract
AIM: To explore the effects of peptide S-8300 from shark liver (S-8300) on liver function as well as in regulating immune functions in experimental injury models.
METHODS: Mice were administered with different doses of S-8300 for four consecutive days, followed by mice injected with tetrachloromethane (CCl4) on d 3. The activity of ALT, AST, LDH, SOD and contents of MDA and GSH in the mice liver were tested. And mice were treated with Cy (100 mg/kg) at the beginning of the experiment to induce immunosuppression model. The S-8300 groups were treated with S-8300 seven days after the beginning of Cy administration. The effects of S-8300 on the formulation of serum hemolysin and the content of IL-2 in serum in the immunosuppression mice were observed.
RESULTS: S-8300 obviously decreased the level of ALT (52.2±11.0 U/dL vs 135.9±6.5 U/dL, P<0.01), AST (67.5±6.9 U/dL vs 238.8±8.7 U/dL, P<0.01), LDH (155.1±46.8 U/dL vs 240.4±6.0 U/dL, P<0.01) & MDA (0.64±0.027 nmol/mg vs 1.06±0.040 nmol/mg, P<0.01) and increased SOD (24.51±1.01 U/mg vs 19.05±0.73 U/mg, P<0.01) & GSH (24.17±0.91 µg/mg vs 14.93±0.45 µg/mg, P<0.01) in mice liver damaged by CCl4. S-8300 also markedly improved the formulation of serum hemolysin (0.094±0.005 A540vs 0.063±0.006 A540, P<0.01) and increased the level of IL-2 (9.74±1.16 pg/mL vs 5.81±0.87 pg/mL, P<0.01) in serum of the immunosuppression mice.
CONCLUSION: The results suggested S-8300 has significant hepatoprotective, immunomodulatory and inhibiting of lipid peroxidation activity.
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Affiliation(s)
- Feng-Jie Huang
- School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, Jiangsu Province, China
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