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Barp L, Miklavčič Višnjevec A, Moret S. Analytical Determination of Squalene in Extra Virgin Olive Oil and Olive Processing By-Products, and Its Valorization as an Ingredient in Functional Food-A Critical Review. Molecules 2024; 29:5201. [PMID: 39519842 PMCID: PMC11547617 DOI: 10.3390/molecules29215201] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/27/2024] [Revised: 10/25/2024] [Accepted: 11/01/2024] [Indexed: 11/16/2024] Open
Abstract
Squalene is a bioactive compound with significant health benefits, predominantly found in extra virgin olive oil (EVOO) and its processing by-products. This critical review explores the analytical determination of squalene in EVOO and various by-products from olive oil production, highlighting its potential as a valuable ingredient in functional foods. An overview of existing analytical methods is provided, focusing on different approaches to sample preparation before analytical determination, evaluating their effectiveness in quantifying squalene concentrations. Studies not primarily centered on analytical methodologies or squalene quantification were excluded. A critical gap identified is the absence of an official method for squalene determination, which hinders comparability and standardization across studies, underscoring the importance of developing a reliable, standardized method to ensure accurate quantification. The valorization of squalene involves advocating for its extraction from olive oil processing by-products to enhance sustainability in the olive oil industry. By recovering squalene, the industry can not only reduce waste but also enhance functional food products with this health-promoting compound. Additionally, there is a need for economically sustainable and environmentally friendly extraction techniques that can be scaled up for industrial application, thus contributing to a circular economy within the olive oil sector.
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Affiliation(s)
- Laura Barp
- Department of Agri-Food, Environmental and Animal Sciences, University of Udine, 33100 Udine, Italy;
| | - Ana Miklavčič Višnjevec
- Faculty of Mathematics, Natural Sciences and Information Technologies, University of Primorska, Glagoljaška 8, 6000 Koper, Slovenia;
| | - Sabrina Moret
- Department of Agri-Food, Environmental and Animal Sciences, University of Udine, 33100 Udine, Italy;
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Vivanco PG, Taboada P, Coelho A. The Southern European Atlantic Diet and Its Supplements: The Chemical Bases of Its Anticancer Properties. Nutrients 2023; 15:4274. [PMID: 37836558 PMCID: PMC10574233 DOI: 10.3390/nu15194274] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/13/2023] [Revised: 09/27/2023] [Accepted: 09/29/2023] [Indexed: 10/15/2023] Open
Abstract
Scientific evidence increasingly supports the strong link between diet and health, acknowledging that a well-balanced diet plays a crucial role in preventing chronic diseases such as obesity, diabetes, cardiovascular issues, and certain types of cancer. This perspective opens the door to developing precision diets, particularly tailored for individuals at risk of developing cancer. It encompasses a vast research area and involves the study of an expanding array of compounds with multilevel "omics" compositions, including genomics, transcriptomics, proteomics, epigenomics, miRNomics, and metabolomics. We review here the components of the Southern European Atlantic Diet (SEAD) from both a chemical and pharmacological standpoint. The information sources consulted, complemented by crystallographic data from the Protein Data Bank, establish a direct link between the SEAD and its anticancer properties. The data collected strongly suggest that SEAD offers an exceptionally healthy profile, particularly due to the presence of beneficial biomolecules in its foods. The inclusion of olive oil and paprika in this diet provides numerous health benefits, and scientific evidence supports the anticancer properties of dietary supplements with biomolecules sourced from vegetables of the brassica genus. Nonetheless, further research is warranted in this field to gain deeper insights into the potential benefits of the SEAD's bioactive compounds against cancer.
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Affiliation(s)
- Pablo García Vivanco
- Spanish Academy of Nutrition and Dietetics, 31006 Pamplona, Spain
- Nutrition and Digestive Working Group, Spanish Society of Clinical, Family, and Community Pharmacy (SEFAC), 28045 Madrid, Spain
| | - Pablo Taboada
- Department of Condensed Matter Physics, Faculty of Physics, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain
- Institute of Materials-USC (IMATUS), University of Santiago de Compostela, 15782 Santiago de Compostela, Spain
| | - Alberto Coelho
- Institute of Materials-USC (IMATUS), University of Santiago de Compostela, 15782 Santiago de Compostela, Spain
- Department of Organic Chemistry, Faculty of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain
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Galani E, Ly I, Laurichesse E, Schmitt V, Xenakis A, Chatzidaki MD. Pea and Soy Protein Stabilized Emulsions: Formulation, Structure, and Stability Studies. COLLOIDS AND INTERFACES 2023. [DOI: 10.3390/colloids7020030] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 04/09/2023]
Abstract
During the last decades, there has been a huge consumer concern about animal proteins that has led to their replacement with plant proteins. Most of those proteins exhibit emulsifying properties; thus, the food industry begins their extensive use in various food matrices. In the present study, pea and soy protein isolates (PPI and SPI) were tested as potential candidates for stabilizing food emulsions to encapsulate α-tocopherol and squalene. More specifically, PPI and SPI particles were formulated using the pH modification method. Following, emulsions were prepared using high-shear homogenization and were observed at both a microscopic and macroscopic level. Furthermore, the adsorption of the proteins was measured using the bicinchoninic acid protein assay. The emulsions’ droplet size as well as their antioxidant capacity were also evaluated. It was found that the droplet diameter of the SPI-based emulsions was 60.0 μm, while the PPI ones had a relatively smaller diameter of approximately 57.9 μm. In the presence of the bioactives, both emulsions showed scavenging activity of the 2,20-Azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical cation (ABTS·+) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals, with the ones loaded with α-tocopherol having the greatest antioxidant capacity. Overall, the proposed systems are very good candidates in different food matrices, with applications ranging from vegan milks and soups to meat alternative products.
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Affiliation(s)
- Eleni Galani
- Institute of Chemical Biology, National Hellenic Research Foundation, 11635 Athens, Greece
- Food Chemistry & Human Nutrition, School of Food, Biotechnology and Development, Agricultural University of Athens, 11855 Athens, Greece
| | - Isabelle Ly
- CNRS Centre de Recherche Paul Pascal, University of Bordeaux, 33600 Bordeaux, France
| | - Eric Laurichesse
- CNRS Centre de Recherche Paul Pascal, University of Bordeaux, 33600 Bordeaux, France
| | - Veronique Schmitt
- CNRS Centre de Recherche Paul Pascal, University of Bordeaux, 33600 Bordeaux, France
| | - Aristotelis Xenakis
- Institute of Chemical Biology, National Hellenic Research Foundation, 11635 Athens, Greece
| | - Maria D. Chatzidaki
- Institute of Chemical Biology, National Hellenic Research Foundation, 11635 Athens, Greece
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Hosni S, Gani SSA, Orsat V, Hassan M, Abdullah S. Ultrasound-Assisted Extraction of Antioxidants from Melastoma malabathricum Linn.: Modeling and Optimization Using Box-Behnken Design. MOLECULES (BASEL, SWITZERLAND) 2023; 28:molecules28020487. [PMID: 36677546 PMCID: PMC9863510 DOI: 10.3390/molecules28020487] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 10/29/2022] [Revised: 12/14/2022] [Accepted: 12/16/2022] [Indexed: 01/06/2023]
Abstract
This study presents modeling and optimization of ultrasound-assisted extraction (UAE) of Melastoma malabathricum with the objective of evaluating its phytochemical properties. This one-factor-at-a-time (OFAT) procedure was conducted to screen for optimization variables whose domains included extraction temperature (XET), ultrasonic time (XUT), solvent concentration (XSC), and sample-to-liquid ratio (XSLR). Response surface methodology (RSM) coupled with Box-Behnken design (BBD) was applied to establish optimum conditions for maximum antioxidant extraction. Modeling and optimization conditions of UAE at 37 kHz, XET 32 °C for XUT 16 min and dissolved in an XSC 70% ethanol concentration at a XSLR 1:10 ratio yielded scavenging effects on 2,2-diphenyl-1-picryl-hydrazyl (DPPH) at 96% ± 1.48 and recorded values of total phenolic content (TPC) and total flavonoid content (TFC) at 803.456 ± 32.48 mg GAE (gallic acid equivalents)/g, and 102.972 ± 2.51 mg QE (quercetin equivalents)/g, respectively. The presence of high flavonoid compounds was verified using TWIMS-QTOFMS. Chromatic evaluation of phytochemicals using gas chromatography-mass spectrometry (GC-MS) revealed the presence of 14 phytocompounds widely documented to play significant roles in human health. This study provides a comparative evaluation with other studies and may be used for validation of the species' potential for its much-acclaimed medicinal and cosmeceutical uses.
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Affiliation(s)
- Suzziyana Hosni
- Halal Products Research Institute, Universiti Putra Malaysia, Seri Kembangan, Serdang 43400, Selangor, Malaysia
| | - Siti Salwa Abd Gani
- Halal Products Research Institute, Universiti Putra Malaysia, Seri Kembangan, Serdang 43400, Selangor, Malaysia
- Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, Seri Kembangan, Serdang 43400, Selangor, Malaysia
- Natural Medicine and Products Research Laboratory, Institute of Biosceince, Universiti Putra Malaysia, Seri Kembangan, Serdang 43400, Selangor, Malaysia
- Correspondence:
| | - Valérie Orsat
- Macdonald Campus, McGill University, Lakeshore Road, Sainte-Anne-de-Bellevue, QC 21111, Canada
| | - Masriana Hassan
- Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Seri Kembangan, Serdang 43400, Selangor, Malaysia
| | - Sumaiyah Abdullah
- Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Seri Kembangan, Serdang 43400, Selangor, Malaysia
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Quan W, Wang A, Gao C, Li C. Applications of Chinese Camellia oleifera and its By-Products: A Review. Front Chem 2022; 10:921246. [PMID: 35685348 PMCID: PMC9171030 DOI: 10.3389/fchem.2022.921246] [Citation(s) in RCA: 29] [Impact Index Per Article: 9.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/15/2022] [Accepted: 05/05/2022] [Indexed: 11/13/2022] Open
Abstract
Camellia oleifera is a woody oil tree species unique to China that has been cultivated and used in China for more than 2,300 years. Most biological research on C. oleifera in recent years has focused on the development of new varieties and breeding. Novel genomic information has been generated for C. oleifera, including a high-quality reference genome at the chromosome level. Camellia seeds are used to process high-quality edible oil; they are also often used in medicine, health foods, and daily chemical products and have shown promise for the treatment and prevention of diseases. C. oleifera by-products, such as camellia seed cake, saponin, and fruit shell are widely used in the daily chemical, dyeing, papermaking, chemical fibre, textile, and pesticide industries. C. oleifera shell can also be used to prepare activated carbon electrodes, which have high electrochemical performance when used as the negative electrode of lithium-ion batteries. C. oleifera is an economically valuable plant with diverse uses, and accelerating the utilization of its by-products will greatly enhance its industrial value.
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Affiliation(s)
- Wenxuan Quan
- Guizhou Provincial Key Laboratory for Information Systems of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, China.,Key Laboratory of Forest Cultivation in Plateau Mountain of Guizhou Province, Institute for Forest Resources and Environment of Guizhou, Guizhou University, Guiyang, China
| | - Anping Wang
- Guizhou Provincial Key Laboratory for Information Systems of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, China
| | - Chao Gao
- Key Laboratory of Forest Cultivation in Plateau Mountain of Guizhou Province, Institute for Forest Resources and Environment of Guizhou, Guizhou University, Guiyang, China
| | - Chaochan Li
- Guizhou Provincial Key Laboratory for Information Systems of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, China
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Recent advances in the microbial production of squalene. World J Microbiol Biotechnol 2022; 38:91. [PMID: 35426523 PMCID: PMC9010451 DOI: 10.1007/s11274-022-03273-w] [Citation(s) in RCA: 22] [Impact Index Per Article: 7.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/17/2022] [Accepted: 03/30/2022] [Indexed: 11/06/2022]
Abstract
Squalene is a triterpene hydrocarbon, a biochemical precursor for all steroids in plants and animals. It is a principal component of human surface lipids, in particular of sebum. Squalene has several applications in the food, pharmaceutical, and medical sectors. It is essentially used as a dietary supplement, vaccine adjuvant, moisturizer, cardio-protective agent, anti-tumor agent and natural antioxidant. With the increased demand for squalene along with regulations on shark-derived squalene, there is a need to find alternatives for squalene production which are low-cost as well as sustainable. Microbial platforms are being considered as a potential option to meet such challenges. Considerable progress has been made using both wild-type and engineered microbial strains for improved productivity and yields of squalene. Native strains for squalene production are usually limited by low growth rates and lesser titers. Metabolic engineering, which is a rational strain engineering tool, has enabled the development of microbial strains such as Saccharomyces cerevisiae and Yarrowia lipolytica, to overproduce the squalene in high titers. This review focuses on key strain engineering strategies involving both in-silico and in-vitro techniques. Emphasis is made on gene manipulations for improved precursor pool, enzyme modifications, cofactor regeneration, up-regulation of limiting reactions, and downregulation of competing reactions during squalene production. Process strategies and challenges related to both upstream and downstream during mass cultivation are detailed.
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Moral R, Escrich E. Influence of Olive Oil and Its Components on Breast Cancer: Molecular Mechanisms. MOLECULES (BASEL, SWITZERLAND) 2022; 27:molecules27020477. [PMID: 35056792 PMCID: PMC8780060 DOI: 10.3390/molecules27020477] [Citation(s) in RCA: 39] [Impact Index Per Article: 13.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 11/24/2021] [Revised: 12/31/2021] [Accepted: 01/08/2022] [Indexed: 02/06/2023]
Abstract
Breast cancer is the most frequent malignant neoplasia and a leading cause of mortality in women worldwide. The Mediterranean diet has been proposed as a healthy dietary pattern with protective effects in several chronic diseases, including breast cancer. This diet is characterized by the consumption of abundant plant foods and olive oil as the principal source of fat, which is considered one of the main components with potential antioxidant, anti-inflammatory and anticancer effects. Extra-virgin olive oil (EVOO) has several bioactive compounds, mainly including monounsaturated fatty acids, triterpenes and polyphenols, such as phenolic alcohols (e.g., hydroxytyrosol), secoiridoids (e.g., oleuropein and oleocanthal), lignans (e.g., pinoresinol) or flavonoids (e.g., luteolin). While epidemiological evidence is still limited, experimental in vivo and in vitro data have shown a protective effect of this oil and its compounds on mammary carcinogenesis. Such effects account through complex and multiple mechanisms, including changes in epigenetics, transcriptome and protein expression that modulate several signaling pathways. Molecular targets of EVOO compounds have a role in the acquisition of cancer hallmarks. Although further research is needed to elucidate their beneficial effects on human prevention and progression of the disease, evidence points to EVOO in the context of the Mediterranean diet as a heathy choice, while EVOO components may be promising adjuvants in anticancer strategies.
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Sánchez-Quesada C, Gutiérrez-Santiago F, Rodríguez-García C, Gaforio JJ. Synergistic Effect of Squalene and Hydroxytyrosol on Highly Invasive MDA-MB-231 Breast Cancer Cells. Nutrients 2022; 14:nu14020255. [PMID: 35057436 PMCID: PMC8780125 DOI: 10.3390/nu14020255] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/17/2021] [Revised: 12/27/2021] [Accepted: 01/05/2022] [Indexed: 12/11/2022] Open
Abstract
Several studies relate Mediterranean diet and virgin olive oil (VOO) intake with lower risk of several chronic diseases, including breast cancer. Many of them described antitumor properties of isolated minor compounds present in VOO, but beneficial properties of VOO arise from the effects of all its compounds acting together. The aim of the present study was to test the antitumor effects of two minor compounds from VOO (hydroxytyrosol (HT) and squalene (SQ)) on highly metastatic human breast tumor cells (MDA-MB-231) when acting in combination. Both isolated compounds were previously analyzed without showing any antitumoral effect on highly invasive MDA-MB-231 breast cancer cells, but the present results show that HT at 100 µM, combined with different concentrations of SQ, could exert antitumor effects. When they are combined, HT and SQ are able to inhibit cell proliferation, promoting apoptosis and DNA damage in metastatic breast cancer cells. Therefore, our results suggest that the health-promoting properties of VOO may be due, at least in part, to the combined action of these two minor compounds.
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Affiliation(s)
- Cristina Sánchez-Quesada
- Department of Health Sciences, Faculty of Experimental Sciences, University of Jaen, 23071 Jaen, Spain; (C.S.-Q.); (F.G.-S.); (C.R.-G.)
- University Institute of Research in Olive Groves and Olive Oils, University of Jaen, Campus las Lagunillas s/n, 23071 Jaen, Spain
- Agri-Food Campus of International Excellence (ceiA3), 14071 Cordoba, Spain
| | - Francisco Gutiérrez-Santiago
- Department of Health Sciences, Faculty of Experimental Sciences, University of Jaen, 23071 Jaen, Spain; (C.S.-Q.); (F.G.-S.); (C.R.-G.)
| | - Carmen Rodríguez-García
- Department of Health Sciences, Faculty of Experimental Sciences, University of Jaen, 23071 Jaen, Spain; (C.S.-Q.); (F.G.-S.); (C.R.-G.)
- University Institute of Research in Olive Groves and Olive Oils, University of Jaen, Campus las Lagunillas s/n, 23071 Jaen, Spain
| | - José J. Gaforio
- Department of Health Sciences, Faculty of Experimental Sciences, University of Jaen, 23071 Jaen, Spain; (C.S.-Q.); (F.G.-S.); (C.R.-G.)
- University Institute of Research in Olive Groves and Olive Oils, University of Jaen, Campus las Lagunillas s/n, 23071 Jaen, Spain
- Agri-Food Campus of International Excellence (ceiA3), 14071 Cordoba, Spain
- CIBER Epidemiología y Salud Pública (CIBER-ESP), Instituto de Salud Carlos III, 28029 Madrid, Spain
- Correspondence: ; Tel.: +34-953-212-002
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Khalifa S, Enomoto M, Kato S, Nakagawa K. Novel Photoinduced Squalene Cyclic Peroxide Identified, Detected, and Quantified in Human Skin Surface Lipids. Antioxidants (Basel) 2021; 10:1760. [PMID: 34829631 PMCID: PMC8614752 DOI: 10.3390/antiox10111760] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/08/2021] [Revised: 10/28/2021] [Accepted: 11/01/2021] [Indexed: 12/20/2022] Open
Abstract
Skin surface lipids (SSLs) form the first barrier that protects the human organism from external stressors, disruption of the homeostasis of SSLs can result in severe skin abnormalities. One of the main causes of this disruption is oxidative stress that is primarily due to SSLs oxidation. Squalene (SQ), the most abundant lipid among SSLs, was shown to first undergo singlet molecular oxygen (1O2) oxidation to yield 6 SQ-monohydroperoxide (SQ-OOH) isomers as the primary oxidation products. However, due to the instability and lability of hydroperoxides, we found that when total SQ-OOH isomers are further photooxidized, they form a unique higher molecular weight secondary oxidation product. To generate the compound, we photooxidized total SQ-OOH isomers in the presence of ground state molecular oxygen (3O2), after its isolation and purification, we studied its structure using MS/MS, NMR, derivatization reactions, and chemical calculations. The compound was identified as 2-OOH-3-(1,2-dioxane)-SQ. Photooxidation of individual SQ-OOH isomers revealed that 6-OOH-SQ is the precursor of 2-OOH-3-(1,2-dioxane)-SQ and indicated the possibility of the formation of similar cyclic peroxides from each isomer following the same photoinduced chain reaction mechanism. An HPLC-MS/MS method was developed for the analysis of 2-OOH-3-(1,2-dioxane)-SQ and its presence on the skin was confirmed in SSLs of six healthy individuals. Its quantity on the skin correlated directly to that of SQ and was not inversely proportional to its precursor, indicating the possibility of its accumulation on the skin surface and the constant regeneration of 6-OOH-SQ from SQ's oxidation. In general, research on lipid cyclic peroxides in the human organism is very limited, and especially on the skin. This study shows for the first time the identification and presence of a novel SQ cyclic peroxide "2-OOH-3-(1,2-dioxane)-SQ" in SSLs, shedding light on the importance of further studying its effect and role on the skin.
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Affiliation(s)
- Saoussane Khalifa
- Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8577, Japan; (S.K.); (S.K.)
| | - Masaru Enomoto
- Applied Bioorganic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8577, Japan;
| | - Shunji Kato
- Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8577, Japan; (S.K.); (S.K.)
| | - Kiyotaka Nakagawa
- Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8577, Japan; (S.K.); (S.K.)
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The Impact of Short-Term Shark Liver Oil Supplementation on the Fatty Acid Composition of Erythrocyte Membranes. Nutrients 2021; 13:nu13103329. [PMID: 34684329 PMCID: PMC8540316 DOI: 10.3390/nu13103329] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/30/2021] [Revised: 09/14/2021] [Accepted: 09/17/2021] [Indexed: 02/06/2023] Open
Abstract
Fatty acid (FA) balance is strictly related to human health. The composition of fatty acids in lipid membranes seems to be influenced by diet. Shark liver oil (SLO) supplementation has been widely used recently in the prevention and treatment of human diseases. We analyzed the impact of short-term SLO supplementation on certain biochemical parameters and erythrocyte FA composition in a group of young healthy women. Our results showed that 6 weeks of SLO supplementation led to a significant decrease in C-reactive protein levels in sera and intracellular cholesterol levels in peripheral blood mononuclear cells. SLO supplementation caused a significant increase in the content of the polyunsaturated omega-3 FAs: docosahexaenoic acid, docosapentaenoic acid and α-linolenic acid. In the group of omega-6 FAs, we observed a significant elevation of arachidonic and dihomo-gamma-linoleic acid content. Due to these alterations, the omega-3 index increased significantly from 3.6% (before) to 4.2% (after supplementation). We also observed the impact of SLO supplementation on the membrane fluidity index. The ratio between saturated and unsaturated FAs decreased significantly from 13.1 to 9.9. In conclusion, our results show that even short-term SLO supplementation can improve human erythrocyte fatty acid composition and other parameters that may have health-promoting consequences.
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Bioprospecting potentials of moderately halophilic bacteria and the isolation of squalene producers from Kuwait sabkha. Int Microbiol 2021; 24:373-384. [PMID: 33755814 DOI: 10.1007/s10123-021-00173-1] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/19/2020] [Revised: 03/07/2021] [Accepted: 03/16/2021] [Indexed: 10/21/2022]
Abstract
Sabkhas in Kuwait are unique hypersaline marine environments under-explored for bacterial community composition and bioprospecting. The 16S rRNA sequence analysis of 46 isolates with distinct morphology from two Kuwait sabkhas recovered 11 genera. Phylum Firmicutes dominated these isolates, and Bacillus (32.6%) was recovered as the dominant genera, followed by Halococcus (17.4%). These isolates were moderately halophilic, and some of them showed tolerance and growth at extreme levels of salt (20%), pH (5 and/or 11), and temperature (55 °C). A higher percentage of isolates harbored protease (63.0), followed by DNase (41.3), amylase (41.3), and lipase (32.6). Selected isolates showed antimicrobial activity against E. faecalis and isolated Halomonas shengliensis, and Idiomarina piscisalsi harbored gene coding for dNDP-glucose 4,6-dehydratase (Glu 1), indicating their potential to produce biomolecules with deoxysugar moieties. Palmitic acid or oleic acid was the dominant fatty acid, and seven isolates had some polyunsaturated fatty acids (linolenic or γ-linolenic acid). Interestingly, six isolates belonging to Planococcus and Oceanobacillus genus produced squalene, a bioactive isoprenoid molecule. Their content increased 30-50% in the presence of Terbinafine. The potential bioactivities and extreme growth conditions make this untapped bacterial diversity a promising candidate for future bioprospecting studies.
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Siger A, Antkowiak W, Dwiecki K, Rokosik E, Rudzińska M. Nutlets of Tilia cordata Mill. and Tilia platyphyllos Scop. - Source of bioactive compounds. Food Chem 2020; 346:128888. [PMID: 33385911 DOI: 10.1016/j.foodchem.2020.128888] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/05/2020] [Revised: 11/23/2020] [Accepted: 12/14/2020] [Indexed: 11/30/2022]
Abstract
The aim of this study was to analyse contents of phytosterols, carotenoids, tocochromanols and fatty acid composition in oil from nutlets of linden. Standard determination methods for these compounds were applied using liquid and gas chromatography. In the analysed oils from small and large-leaved lime the dominant acids included linoleic (53.1 and 54.1%), oleic (18.5 and 22.2%), palmitic (15.3 and 11.8%). Linden seed oil was characterised by very high contents of phytosterols (2-2.5 g/100 g). β-Sitosterol was the dominant phytosterol (80-83%). Additionally squalene was detected at 806 and 607 mg/100 g, respectively, for Tilia cordata and T.platyphyllos. It was found that linden seed oil contains tocopherols (93%) and tocotrienols (7%). In terms of tocochromanol contents this oil is surpassed only by wheat germ oil. In terms of the α-T equivalent these oils contain 95 (Tilia cordata) and 50.6 mg/100 g vitamin E (Tilia platyphyllos).
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Affiliation(s)
- Aleksander Siger
- Department of Food Biochemistry and Analysis, Poznań University of Life Sciences, Wojska Polskiego 28, 60-637 Poznań, Poland.
| | - Wojciech Antkowiak
- Department of Botany, Poznań University of Life Sciences, Wojska Polskiego 71 c, 60-637 Poznań, Poland
| | - Krzysztof Dwiecki
- Department of Food Biochemistry and Analysis, Poznań University of Life Sciences, Wojska Polskiego 28, 60-637 Poznań, Poland
| | - Ewa Rokosik
- Department of Food Biochemistry and Analysis, Poznań University of Life Sciences, Wojska Polskiego 28, 60-637 Poznań, Poland
| | - Magdalena Rudzińska
- Institute of Food Technology, Poznań University of Life Sciences, Wojska Polskiego 28, 60-637 Poznań, Poland
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Toopkanloo SP, Tan TB, Abas F, Azam M, Nehdi IA, Tan CP. Improving Vesicular Integrity and Antioxidant Activity of Novel Mixed Soy Lecithin-Based Liposomes Containing Squalene and Their Stability against UV Light. Molecules 2020; 25:E5873. [PMID: 33322600 PMCID: PMC7764204 DOI: 10.3390/molecules25245873] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/30/2020] [Revised: 12/08/2020] [Accepted: 12/09/2020] [Indexed: 11/18/2022] Open
Abstract
In order to improve the membrane lipophilicity and the affinity towards the environment of lipid bilayers, squalene (SQ) could be conjugated to phospholipids in the formation of liposomes. The effect of membrane composition and concentrations on the degradation of liposomes prepared via the extrusion method was investigated. Liposomes were prepared using a mixture of SQ, cholesterol (CH) and Tween80 (TW80). Based on the optimal conditions, liposome batches were prepared in the absence and presence of SQ. Their physicochemical and stability behavior were evaluated as a function of liposome constituent. From the optimization study, the liposomal formulation containing 5% (w/w) mixed soy lecithin (ML), 0.5% (w/w) SQ, 0.3% (w/w) CH and 0.75% (w/w) TW80 had optimal physicochemical properties and displayed a unilamellar structure. Liposome prepared using the optimal formulation had a low particle size (158.31 ± 2.96 nm) and acceptable %increase in the particle size (15.09% ± 3.76%) and %trolox equivalent antioxidant capacity (%TEAC) loss (35.69% ± 0.72%) against UV light treatment (280-320 nm) for 6 h. The interesting outcome of this research was the association of naturally occurring substance SQ for size reduction without the extra input of energy or mechanical procedures, and improvement of vesicle stability and antioxidant activity of ML-based liposome. This study also demonstrated that the presence of SQ in the membrane might increase the acyl chain dynamics and decrease the viscosity of the dispersion, thereby limiting long-term stability of the liposome.
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Affiliation(s)
- Sahar Pakbaten Toopkanloo
- Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia;
| | - Tai Boon Tan
- Department of Food Service and Management, Faculty of Food Science and Technology, Universiti Putra Malaysia, UPM, Serdang 43400, Selangor, Malaysia;
| | - Faridah Abas
- Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, UPM, Serdang 43400, Selangor, Malaysia;
| | - Mohammad Azam
- Chemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia; (M.A.); (I.A.N.)
| | - Imededdine Arbi Nehdi
- Chemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia; (M.A.); (I.A.N.)
- Chemistry Department, El Manar Preparatory Institute for Engineering Studies, Tunis El Manar University, P.O. Box 244, Tunis 2092, Tunisia
| | - Chin Ping Tan
- Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia;
- Laboratory of Processing and Product Development, Institute of Plantation Studies, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
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14
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Arendowski A, Ossolinski K, Niziol J, Ruman T. Screening of Urinary Renal Cancer Metabolic Biomarkers with Gold Nanoparticles-assisted Laser Desorption/Ionization Mass Spectrometry. ANAL SCI 2020; 36:1521-1525. [PMID: 32830161 DOI: 10.2116/analsci.20p226] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/25/2020] [Accepted: 08/13/2020] [Indexed: 08/09/2023]
Abstract
Renal cell carcinoma is a very aggressive and often fatal disease for which there are no specific biomarkers found to date. The purpose of this work was to find features that differentiate urine metabolic profiles of healthy people and cancer patients. Laser desorption/ionization mass spectrometry on gold nanostructures-based techniques were used for the metabolic analysis of urine of 50 patients with kidney cancer. Comparison with data from 50 healthy volunteers led to the discovery of several compounds that may be considered potential renal cell carcinoma (RCC) biomarkers. Statistical analysis of data allowed for the discovery of m/z values that had the greatest impact on group differentiation. A database search enabled the assignment of signals for the most promising 15 features among them: serine, heptanol, 3-methylene-indolenine, 2-methyl-3-hydroxy-5-formylpyridine-4-carboxylate, phosphodimethylethanolamine, 4-methoxyphenylacetic acid, N-acetylglutamine, 3,5-dihydroxyphenylvaleric acid, hydroxyhexanoylglycine, valyl-leucine, leucyl-histidine, oleamide, 9,12,13-trihydroxyoctadecenoic acid, stearidonyl carnitine and squalene. Differences of metabolite profiles of human urine could be identified by gold nanoparticle-enhanced target (AuNPET) LDI MS method and used for the detection of renal cancer.
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Affiliation(s)
- Adrian Arendowski
- Faculty of Chemistry, Rzeszów University of Technology, 6 Powstańców Warszawy Ave, 35-959, Rzeszów, Poland
| | - Krzysztof Ossolinski
- Department of Urology, John Paul II District Hospital, Grunwaldzka 4 St, 36-100, Kolbuszowa, Poland
| | - Joanna Niziol
- Faculty of Chemistry, Rzeszów University of Technology, 6 Powstańców Warszawy Ave, 35-959, Rzeszów, Poland
| | - Tomasz Ruman
- Faculty of Chemistry, Rzeszów University of Technology, 6 Powstańców Warszawy Ave, 35-959, Rzeszów, Poland
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15
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Beneficial effects of olive oil and Mediterranean diet on cancer physio-pathology and incidence. Semin Cancer Biol 2020; 73:178-195. [PMID: 33249203 DOI: 10.1016/j.semcancer.2020.11.011] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/24/2020] [Revised: 11/16/2020] [Accepted: 11/17/2020] [Indexed: 12/13/2022]
Abstract
Virgin olive oil is a characteristic component and the main source of fat of the Mediterranean diet. It is a mix of high-value health compounds, including monounsaturated fatty acids (mainly oleic acid), simple phenols (such as hydroxytyrosol and tyrosol), secoiridoids (such as oleuropein, oleocanthal), flavonoids, and terpenoids (such as squalene). Olive oil consumption has been shown to improve different aspects of human health and has been associated with a lower risk of cancer. However, the underlying cellular mechanisms involved in such effects are still poorly defined, but seem to be related to a promotion of apoptosis, modulation of epigenetic patterns, blockade of cell cycle, and angiogenesis regulation. The aim of this review is to update the current associations of cancer risk with the Mediterranean diet, olive oil consumption and its main components. In addition, the identification of key olive oil components involved in anticarcinogenic mechanisms and pathways according to experimental models is also addressed.
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16
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Rajamani K, Thirugnanasambandan SS, Natesan C, Subramaniam S, Thangavel B, Aravindan N. Squalene deters drivers of RCC disease progression beyond VHL status. Cell Biol Toxicol 2020; 37:611-631. [PMID: 33219891 DOI: 10.1007/s10565-020-09566-w] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/04/2020] [Accepted: 10/26/2020] [Indexed: 12/12/2022]
Abstract
Identifying drug candidates to target cellular events/signaling that evades von Hippel-Lindau tumor suppressor (VHL) gene interaction is critical for the cure of renal cell carcinoma (RCC). Recently, we characterized a triterpene-squalene derived from marine brown alga. Herein, we investigated the potential of squalene in targeting HIF-signaling and other drivers of RCC progression. Squalene inhibited cell proliferation, induced cell dealth and reverted the cells' metastatic state (migration, clonal expansion) independent of their VHL status. Near-identical inhibition of HIF-1α and HIF-2α and the regulation of downstream targets in VHL wild type and mutant cell lines demonstrated squalene efficacy beyond VHL-HIF interaction. In a rat model of chemically induced RCC, squalene displayed chemopreventive capabilities by substantial reversal of lipid peroxidation, mitochondrial redox regulation, maintaining ∆ψm, inflammation [Akt, nuclear factor κB (NF-κB)], angiogenesis (VEGFα), metastasis [matrix metalloproteinase 2 (MMP-2)], and survival (Bax/Bcl2, cytochrome-c, Casp3). Squalene restored glutathione, glutathione reductase, glutathione-s-transferase, catalase, and superoxide dismutase and stabilized alkaline phosphatase, alkaline transaminase, and aspartate transaminase. The correlation of thiobarbituric acid reactive substance with VEGF/NF-κB and negative association of GSH with Casp3 show that squalene employs reduction in ROS regulation. Cytokinesis-block micronuclei (CBMN) assay in VHLwt/mut cells revealed both direct and bystander effects of squalene with increased micronucleus (MN) frequency. Clastogenicity analysis of rat bone marrow cells demonstrated an anti-clastogenic effect of squalene, with increased polychromatic erythrocytes (PCEs), decreased MNPCE,s and MN normochromatic erythrocytes. Squalene could effectively target HIF signaling that orchestrate RCC evolution. The efficacy of squalene is similar in VHLwt and VHLmut RCC cells, and hence, squalene could serve as a promising drug candidate for an RCC cure beyond VHL status and VHL-HIF interaction dependency. Summary: Squalene derived from marine brown algae displays strong anti-cancer (RCC) activity, functionally targeting HIF-signaling pathway, and affects various cellular process. The significance of squalene effect for RCC is highlighted by its efficiency beyond VHL status, designating itself a promising drug candidate. Graphical abstract.
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Affiliation(s)
- Karthikeyan Rajamani
- Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai, 608502, TN, India.
- Rajah Muthiah Medical College, Annamalai University, Annamalai Nagar, Chidambaram, 608002, TN, India.
- WHO Collaborating Center for Occupational and Environmental Health, ICMR Center for Air Quality, Climate and Health, Department of Environmental Health Engineering, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, TN, 600116, India.
| | | | - Chidambaram Natesan
- Rajah Muthiah Medical College, Annamalai University, Annamalai Nagar, Chidambaram, 608002, TN, India
| | - Sethupathy Subramaniam
- Rajah Muthiah Medical College, Annamalai University, Annamalai Nagar, Chidambaram, 608002, TN, India
| | | | - Natarajan Aravindan
- Department of Radiation Oncology, University of Oklahoma Health Sciences Center, BMSB 737, 947 Stanton L. Young Boulevard, Oklahoma City, OK, 73104, USA.
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17
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Yu W, Sun K, Zhang L, Wan X, Chen C, Su R, Liu Y, Wang H, Yang H. Investigation of the Effects of Squalene and Squalene Epoxides on the Homeostasis of Coenzyme Q10 in Rats by UPLC‐Orbitrap MS. Chem Biodivers 2020; 17:e2000243. [DOI: 10.1002/cbdv.202000243] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/30/2020] [Accepted: 05/29/2020] [Indexed: 11/12/2022]
Affiliation(s)
- Wenjing Yu
- Changchun University of Chinese Medicine 1035 Boshuo Road Changchun 130117 P. R. China
| | - Kaiju Sun
- Changchun University of Chinese Medicine 1035 Boshuo Road Changchun 130117 P. R. China
| | - Liying Zhang
- Changchun University of Chinese Medicine 1035 Boshuo Road Changchun 130117 P. R. China
| | - Xilin Wan
- Changchun University of Chinese Medicine 1035 Boshuo Road Changchun 130117 P. R. China
| | - Changbao Chen
- Changchun University of Chinese Medicine 1035 Boshuo Road Changchun 130117 P. R. China
| | - Rui Su
- Changchun University of Chinese Medicine 1035 Boshuo Road Changchun 130117 P. R. China
| | - Yifei Liu
- Anhui Pujia Medical Technology Co.Ltd. 2800 Chuangxin Road Hefei 230000 P. R. China
| | - Hongfeng Wang
- Changchun University of Chinese Medicine 1035 Boshuo Road Changchun 130117 P. R. China
| | - Hongmei Yang
- Changchun University of Chinese Medicine 1035 Boshuo Road Changchun 130117 P. R. China
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18
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Cloning, functional characterization and expression analysis of LoTPS5 from Lilium 'Siberia'. Gene 2020; 756:144921. [PMID: 32593719 DOI: 10.1016/j.gene.2020.144921] [Citation(s) in RCA: 16] [Impact Index Per Article: 3.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/21/2020] [Revised: 06/08/2020] [Accepted: 06/20/2020] [Indexed: 02/05/2023]
Abstract
Lilium 'Siberia' is a perennial herbaceous plant that is commercially significant because of its snowy white floral color and appealing scent which is mainly due to the presence of monoterpenes and benzoids compounds in floral volatile profile. In the current study, LoTPS5 was cloned and functionally characterized. Results revealed that LoTPS5 specifically generates squalene from FPP, whereas no product was produced when it was incubated with GPP or GGPP. The subcellular localization experiment showed that LoTPS5 was located in plastids. Furthermore, LoTPS5 showed its high expression in the leaf followed by petals and sepals of the flower. Moreover, the expression of LoTPS5 gradually increased from the bud stage and peak at the full-bloom stage. Besides, LoTPS5 showed a diurnal circadian rhythmic pattern with a peak in the afternoon (16:00) followed by deep night (24:00) and morning (8:00), respectively. LoTPS5 is highly responsive to mechanical wounding by rapidly elevating its mRNA transcript level. The current study will provide significant information for future studies of terpenoid and squalene biosynthesis in Lilium 'Siberia'.
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19
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Park SY, Choi SJ, Park HJ, Ma SY, Moon YI, Park SK, Jung MY. Hexane extract of green tea ( Camellia sinensis) leaves is an exceptionally rich source of squalene. Food Sci Biotechnol 2020; 29:769-775. [PMID: 32523786 DOI: 10.1007/s10068-019-00724-3] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/07/2019] [Revised: 11/18/2019] [Accepted: 12/10/2019] [Indexed: 11/25/2022] Open
Abstract
Squalene is a cancer chemo-preventive and skin protective agent with high commercial demand. Here, we report for the first time that the green tea (Camellia sinensis) leaves is a surprisingly rich plant-based source of squalene. Young and tender leaves and old and turf leaves were collected at four different collecting seasons (April-August). Lipophilic compounds in the leaves and commercial green teas were extracted with hexane. The squalene contents in the hexane extracts varied greatly with the types of the leaves and collecting seasons. The hexane extract of turf leaves contained significantly higher contents of squalene than the extract of tender leaves. The hexane extract of the turf leaves collected in August contained the highest content of squalene (29.2 g/kg extract). This represents the first report on the qualitative and quantitative information on squalene in green tea leaves.
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Affiliation(s)
- Su Yeon Park
- Department of Food and Biotechnology, Graduate School, College of Food Science, Woosuk University, Samrea-up, Wanju-gun, Jeonbuk Province 565-701 Republic of Korea
| | - Sol Ji Choi
- Department of Food and Biotechnology, Graduate School, College of Food Science, Woosuk University, Samrea-up, Wanju-gun, Jeonbuk Province 565-701 Republic of Korea
| | - Hee Jeong Park
- Department of Food and Biotechnology, Graduate School, College of Food Science, Woosuk University, Samrea-up, Wanju-gun, Jeonbuk Province 565-701 Republic of Korea
| | - Sang Yong Ma
- Department of Food and Biotechnology, Graduate School, College of Food Science, Woosuk University, Samrea-up, Wanju-gun, Jeonbuk Province 565-701 Republic of Korea
| | - Yong Il Moon
- Department of Food Industry and Culinary Arts, Woosuk University, Wanju-gun, Jeonbuk Province Republic of Korea
| | - Sang-Kyu Park
- Department of Food Nutrition, Nambu University, Gwangju, Republic of Korea
| | - Mun Yhung Jung
- Department of Food and Biotechnology, Graduate School, College of Food Science, Woosuk University, Samrea-up, Wanju-gun, Jeonbuk Province 565-701 Republic of Korea
- Department of Food Industry and Culinary Arts, Woosuk University, Wanju-gun, Jeonbuk Province Republic of Korea
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20
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Toderich KN, Mamadrahimov AA, Khaitov BB, Karimov AA, Soliev AA, Nanduri KR, Shuyskaya EV. Differential Impact of Salinity Stress on Seeds Minerals, Storage Proteins, Fatty Acids, and Squalene Composition of New Quinoa Genotype, Grown in Hyper-Arid Desert Environments. FRONTIERS IN PLANT SCIENCE 2020; 11:607102. [PMID: 33365043 PMCID: PMC7750330 DOI: 10.3389/fpls.2020.607102] [Citation(s) in RCA: 13] [Impact Index Per Article: 2.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/16/2020] [Accepted: 11/09/2020] [Indexed: 05/13/2023]
Abstract
The effects of climate change and soil salinization on dryland ecosystems are already widespread, and ensuring food security is a crucial challenge. In this article, we demonstrate changes in growth performance and seed quality of a new high-yielding quinoa genotype (Q5) exposed to sodium chloride (NaCl), sodium sulfate (Na2SO4), and mixed salts (NaCl + Na2SO4). Differential responses to salt stress in growth performance, seed yield, and seed quality were identified. High salinity (mixed Na2SO4 + NaCl) reduces plant height by ∼30%, shoot and root dry weights by ∼29%, head panicle length and panicle weight by 36-43%, and seed yield by 37%, compared with control conditions. However, the 1,000-seed weight changes insignificantly under salinity. High content of essential minerals, such as Fe, Zn, and Ca in quinoa Q5 seeds produced under salinity, gives the Q5 genotype a remarkable advantage for human consumption. Biomarkers detected in our studies show that the content of most essential amino acids is unchanged under salinity. The content of amino acids Pro, Gly, and Ile positively correlates with Na+ concentration in soil and seeds, whereas the content of squalene and most fatty acids negatively correlates. Variation in squalene content under increasing salinity is most likely due to toxic effects of sodium and chlorine ions as a result of the decrease in membrane permeability for ion movement as a protective reaction to an increase in the sodium ion concentration. Low squalene accumulation might also occur to redirect the NADPH cofactor to enhance the biosynthesis of proline in response to salinity, as both syntheses (squalene and proline) require NADPH. This evidence can potentially be used by the food and pharmaceutical industries in the development of new food and health products.
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Affiliation(s)
- Kristina N. Toderich
- International Platform for Dryland Research and Education, Tottori University, Tottori, Japan
- International Center for Biosaline Agriculture for Central Asia and Caucasus (ICBA-CAC), Tashkent, Uzbekistan
| | | | - Botir B. Khaitov
- International Center for Biosaline Agriculture for Central Asia and Caucasus (ICBA-CAC), Tashkent, Uzbekistan
| | - Aziz A. Karimov
- International Center for Biosaline Agriculture for Central Asia and Caucasus (ICBA-CAC), Tashkent, Uzbekistan
| | - Azamjon A. Soliev
- Institute of Bioorganic Chemistry Academy of Sciences of Uzbekistan, Tashkent, Uzbekistan
| | - Kameswara Rao Nanduri
- International Center for Biosaline Agriculture for Central Asia and Caucasus (ICBA-CAC), Tashkent, Uzbekistan
| | - Elena V. Shuyskaya
- K.A. Timiryazev Institute of Plant Physiology Russian Academy of Sciences, Moscow, Russia
- *Correspondence: Elena V. Shuyskaya,
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21
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Martakos I, Kostakis M, Dasenaki M, Pentogennis M, Thomaidis N. Simultaneous Determination of Pigments, Tocopherols, and Squalene in Greek Olive Oils: A Study of the Influence of Cultivation and Oil-Production Parameters. Foods 2019; 9:foods9010031. [PMID: 31905742 PMCID: PMC7023276 DOI: 10.3390/foods9010031] [Citation(s) in RCA: 21] [Impact Index Per Article: 3.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/04/2019] [Revised: 12/17/2019] [Accepted: 12/17/2019] [Indexed: 12/19/2022] Open
Abstract
A new facile and fast method was developed in this study for the determination of pigments (chlorophylls and carotenoids), tocopherols (α-, sum of (β + γ), and δ), and squalene in olive oil. This method consisted of a dilution of olive oil in 2-propanol, followed by reversed phase-high-pressure liquid chromatography equipped with a diode array detector (RP-HPLC-DAD). Chromatographic separation was performed using a C18 column, while the mobile phase consisted of acetonitrile and methanol using a gradient elution program. The methodology was optimized, validated, and applied to the analysis of 452 samples of Extra Virgin Olive Oil (EVOOs) and Virgin Olive Oil (VOOs) originated from five islands of the Northeastern Aegean Region, in Greece. From the obtained results, it was indicated that the carotenoid, tocopherol, and squalene content was relatively high, while the chlorophyll content was low. Furthermore, the acquired results were studied and compared in order to obtain useful information about the correlation of the concentration levels of these compounds in olive oil to different cultivation and olive oil production parameters. Several parameters were found to play a significant role on the pigment and antioxidant content of olive oil, such as the olive tree variety, geographical origin, fruit maturation stage during harvesting, and addition of water during malaxation, while other parameters such as the altitude of cultivation, the type of farming (organic or conventional), and the type of olive mill did not seem to affect the levels of these compounds.
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22
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Sakul A, Ozansoy M, Elibol B, Ayla Ş, Günal MY, Yozgat Y, Başağa H, Şahin K, Kazancioğlu R, Kiliç Ü. Squalene attenuates the oxidative stress and activates AKT/mTOR pathway against cisplatin-induced kidney damage in mice. Turk J Biol 2019; 43:179-188. [PMID: 31320816 PMCID: PMC6620038 DOI: 10.3906/biy-1902-77] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/01/2023] Open
Abstract
The clinical use of cisplatin, which is a first-line anticancer agent, is highly restricted due to its adverse effects on kidneys that lead to nephrotoxicity. Therefore, some potential reno-protective substances have been used in combination with cisplatin to cope with nephrotoxicity. Due to its high antitumor activity and oxygen-carrying capacity, we investigated the molecular effects of squalene against cisplatin-induced oxidative stress and kidney damage in mice. Single dose of cisplatin (7 mg/kg) was given to male Balb/c mice. Squalene (100 mg/kg/day) was administered orogastrically to mice for 10 days. Following sacrification, molecular alterations were investigated as analysis of the levels of oxidative stress index (OSI), inflammatory cytokines and cell survival-related proteins in addition to histopathological examinations in mice kidney tissue. The level OSI and Interferon-gamma (IFN-γ) decreased in the cisplatin and squalene cotreated mice compared to cisplatin-treated mice. Squalene treatment also increased the activation of protein kinase B (AKT). Furthermore, cisplatin-induced inactivation of mammalian target of rapamycin (mTOR) and histopathological damages were reversed by squalene. It may be suggested that squalene ameliorated the cisplatin-induced histopathological damages in the kidney through activation of AKT/mTOR signaling pathway by regulating the balance of the redox system due to its antioxidative effect.
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Affiliation(s)
- Arzu Sakul
- Department of Medical Pharmacology, School of Medicine, İstanbul Medipol University, İstanbul, Turkey
| | - Mehmet Ozansoy
- Department of Physiology, School of Medicine, İstanbul Medipol University, İstanbul, Turkey
| | - Birsen Elibol
- Department of Medical Biology, Faculty of Medicine, Bezmialem Vakıf University, İstanbul, Turkey
| | - Şule Ayla
- Department of Histology and Embryology, School of Medicine, İstanbul Medipol University, İstanbul, Turkey
| | - Mehmet Yalçın Günal
- Department of Physiology, Alanya Alaaddin Keykubat University School of Medicine, Antalya, Turkey
| | - Yasemin Yozgat
- Regenerative and Restorative Medical Research Center (REMER), İstanbul Medipol University, İstanbul, Turkey
| | - Hüveyda Başağa
- Biological Sciences and Bioengineering Program, Faculty of Engineering and Natural Sciences, Sabancı University, İstanbul, Turkey
| | - Kazım Şahin
- Department of Animal Nutrition, Faculty of Veterinary Medicine, Fırat University, Elazığ, Turkey
| | - Rümeyza Kazancioğlu
- Department of Nephrology, Faculty of Medicine, Bezmialem Vakıf University, İstanbul, Turkey
| | - Ülkan Kiliç
- Department of Nephrology, Faculty of Medicine, Bezmialem Vakıf University, İstanbul, Turkey
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23
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Song Y, Zhang W, Wu J, Admassu H, Liu J, Zhao W, Yang R. Ethanol‐Assisted Aqueous Enzymatic Extraction of Peony Seed Oil. J AM OIL CHEM SOC 2019. [DOI: 10.1002/aocs.12204] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/08/2022]
Affiliation(s)
- Yuanyuan Song
- State Key Laboratory of Food Science & TechnologyJiangnan University 1800 Lihu Avenue, Wuxi 214122 China
- School of Food Science and TechnologyJiangnan University 1800 Lihu Avenue, Wuxi 214122 China
| | - Wenbin Zhang
- State Key Laboratory of Food Science & TechnologyJiangnan University 1800 Lihu Avenue, Wuxi 214122 China
- School of Food Science and TechnologyJiangnan University 1800 Lihu Avenue, Wuxi 214122 China
| | - Jing Wu
- Jiangxi Lvyexuan Biotechnology Co., Ltd. Jinxin 1 Road, Ruijin 342500 China
| | - Habtamu Admassu
- School of Food Science and TechnologyJiangnan University 1800 Lihu Avenue, Wuxi 214122 China
| | - Junjun Liu
- State Key Laboratory of Food Science & TechnologyJiangnan University 1800 Lihu Avenue, Wuxi 214122 China
- School of Food Science and TechnologyJiangnan University 1800 Lihu Avenue, Wuxi 214122 China
| | - Wei Zhao
- State Key Laboratory of Food Science & TechnologyJiangnan University 1800 Lihu Avenue, Wuxi 214122 China
- School of Food Science and TechnologyJiangnan University 1800 Lihu Avenue, Wuxi 214122 China
| | - Ruijin Yang
- State Key Laboratory of Food Science & TechnologyJiangnan University 1800 Lihu Avenue, Wuxi 214122 China
- School of Food Science and TechnologyJiangnan University 1800 Lihu Avenue, Wuxi 214122 China
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24
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Olive Oil Effects on Colorectal Cancer. Nutrients 2018; 11:nu11010032. [PMID: 30583613 PMCID: PMC6357067 DOI: 10.3390/nu11010032] [Citation(s) in RCA: 69] [Impact Index Per Article: 9.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/28/2018] [Revised: 12/11/2018] [Accepted: 12/15/2018] [Indexed: 02/08/2023] Open
Abstract
Colorectal cancer is the fourth cause of cancer-related death worldwide. A Mediterranean diet showed protective action against colorectal cancer due to the intake of different substances. Olive oil is a fundamental component of the Mediterranean diet. Olive oil is rich in high-value health compounds (such as monounsaturated free fatty acids, squalene, phytosterols, and phenols). Phenolic compounds exert favourable effects on free radicals, inflammation, gut microbiota, and carcinogenesis. The interaction between gut microbiota and olive oil consumption could modulate colonic microbial composition or activity, with a possible role in cancer prevention. Gut microbiota is able to degrade some substances found in olive oil, producing active metabolites with chemopreventive action. Further clinical research is needed to clarify the beneficial effects of olive oil and its components. A better knowledge of the compounds found in olive oil could lead to the development of nutritional supplements or chemotherapeutic agents with a potential in the prevention and treatment of colorectal cancer.
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Kim YJ, Lee Y, Kim J, Park SY, Lee K, Hwang KT. Physicochemical characteristics and anti-oxidant activities of farm-cultivated and mountain-cultivated ginseng seeds. Food Sci Biotechnol 2018; 27:1257-1264. [PMID: 30319833 DOI: 10.1007/s10068-018-0363-8] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/17/2017] [Revised: 02/07/2018] [Accepted: 03/25/2018] [Indexed: 11/28/2022] Open
Abstract
Physicochemical characteristics and anti-oxidant capacities of seeds from two farm-cultivated and one mountain-cultivated ginsengs (Panax ginseng Meyer) (MG) were determined. The seeds had 17.9-22.1% (dry basis) crude lipids, 11.5-15.2% crude proteins, and 1.4-1.7% ash. Oleic acid (77.9-78.5%) was the predominant fatty acid in the seed oils, followed by linoleic acid (16.6-17.4%). The highest Hunter b value, carotenoids, (β + γ)-tocotrienol, and δ-tocotrienol, and the lowest α-tocotrienol were observed in the seed oils from MG. Squalene was also the most abundant in the MG seed oils. β-Sitosterol was the major phytosterol in the seed oils with MG the highest. Defatted seed meal extracts from MG possessed the most total phenolics and flavonoids, and the highest DPPH and ABTS radical scavenging activities. These results suggest that MG seeds may be a novel source of functional foods.
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Affiliation(s)
- Yu Jeong Kim
- Department of Food and Nutrition and Research Institute of Human Ecology, Seoul National University, Seoul, 08826 Korea
| | - Yongcheol Lee
- Department of Food and Nutrition and Research Institute of Human Ecology, Seoul National University, Seoul, 08826 Korea
| | - Jaecheol Kim
- Department of Food and Nutrition and Research Institute of Human Ecology, Seoul National University, Seoul, 08826 Korea
| | - Sun Young Park
- Department of Food and Nutrition and Research Institute of Human Ecology, Seoul National University, Seoul, 08826 Korea
| | - Kiuk Lee
- Department of Food and Nutrition and Research Institute of Human Ecology, Seoul National University, Seoul, 08826 Korea
| | - Keum Taek Hwang
- Department of Food and Nutrition and Research Institute of Human Ecology, Seoul National University, Seoul, 08826 Korea
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26
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Lombardo L, Grasso F, Lanciano F, Loria S, Monetti E. Broad-Spectrum Health Protection of Extra Virgin Olive Oil Compounds. ACTA ACUST UNITED AC 2018. [DOI: 10.1016/b978-0-444-64057-4.00002-8] [Citation(s) in RCA: 17] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 03/21/2023]
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27
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Quantification of Squalene in Olive Oil Using 13C Nuclear Magnetic Resonance Spectroscopy. MAGNETOCHEMISTRY 2017. [DOI: 10.3390/magnetochemistry3040034] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/16/2022]
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28
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Khallouki F, Eddouks M, Mourad A, Breuer A, Owen RW. Ethnobotanic, Ethnopharmacologic Aspects and New Phytochemical Insights into Moroccan Argan Fruits. Int J Mol Sci 2017; 18:2277. [PMID: 29084170 PMCID: PMC5713247 DOI: 10.3390/ijms18112277] [Citation(s) in RCA: 16] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/28/2017] [Revised: 10/20/2017] [Accepted: 10/27/2017] [Indexed: 12/31/2022] Open
Abstract
This review summarizes available data on argan fruit botany, geographical distribution, traditional uses, environmental interest, socioeconomic role, phytochemistry, as well as health beneficial effects and examination of future prospects. In particular, ethnomedical uses of argan fruits are carried out throughout Morocco where it has been used against various diseases. Different classes of bioactive compounds have been characterized including essential oils, fatty acids, triacylglycerols, flavonoids and their newly reported acylglycosyl derivatives, monophenols, phenolic acids, cinnamic acids, saponins, triterpenes, phytosterols, ubiquinone, melatonin, new aminophenols along with vitamin E among other secondary metabolites. The latter have already shown a wide spectrum of in vitro, and ex vivo biologicalactivities including antioxidant, anti-inflammatory, anti-diabetic, antihypertensive, anti-hypercholesterolemia, analgesic, antimicrobial, molluscicidal anti-nociceptive and anticancer potential. Argan flesh (pulp) contains a broad spectrum of polyphenolic compounds which may have utility for incorporation into nutraceuticals and cosmeceuticals relevant to the food, cosmetic and health industries. Further research is recommended, especially on the health beneficial effects of the aminophenols.
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Affiliation(s)
- Farid Khallouki
- Divisionof Preventive Oncology, National Center for Tumor Diseases, Im Neuenheimer Feld 460, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 581, D-69120 Heidelberg, Germany.
- Team of Physiology, Nutrition and Endocrinology, Biology Department, FSTE, BP 509 Boutalamine, Errachidia, Morocco.
| | - Mohamed Eddouks
- Team of Physiology, Nutrition and Endocrinology, Biology Department, FSTE, BP 509 Boutalamine, Errachidia, Morocco.
| | - Akdad Mourad
- Team of Physiology, Nutrition and Endocrinology, Biology Department, FSTE, BP 509 Boutalamine, Errachidia, Morocco.
| | - Andrea Breuer
- Divisionof Preventive Oncology, National Center for Tumor Diseases, Im Neuenheimer Feld 460, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 581, D-69120 Heidelberg, Germany.
| | - Robert Wyn Owen
- Divisionof Preventive Oncology, National Center for Tumor Diseases, Im Neuenheimer Feld 460, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 581, D-69120 Heidelberg, Germany.
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29
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Biotechnological production of value-added compounds by ustilaginomycetous yeasts. Appl Microbiol Biotechnol 2017; 101:7789-7809. [DOI: 10.1007/s00253-017-8516-x] [Citation(s) in RCA: 18] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/04/2017] [Revised: 09/03/2017] [Accepted: 09/04/2017] [Indexed: 11/30/2022]
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30
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Rotondo A, Salvo A, Gallo V, Rastrelli L, Dugo G. Quick unreferenced NMR quantification of Squalene in vegetable oils. EUR J LIPID SCI TECH 2017. [DOI: 10.1002/ejlt.201700151] [Citation(s) in RCA: 31] [Impact Index Per Article: 3.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/10/2022]
Affiliation(s)
- Archimede Rotondo
- Dipartimento di BIOMORF- Università di Messina; Viale Annunziata Ex Veterinaria; Messina Italy
| | - Andrea Salvo
- Dipartimento di BIOMORF- Università di Messina; Viale Annunziata Ex Veterinaria; Messina Italy
| | - Vito Gallo
- Dipartimento di Ingegneria Civile, Ambientale, del Territorio; Edile e di Chimica−Politecnico di Bari; Bari Italy
| | - Luca Rastrelli
- Dipartimento di Farmacia DIFARMA; Università di Salerno; Fisciano (SA) Italy
| | - Giacomo Dugo
- Dipartimento di BIOMORF- Università di Messina; Viale Annunziata Ex Veterinaria; Messina Italy
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31
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Chiou A, Kalogeropoulos N. Virgin Olive Oil as Frying Oil. Compr Rev Food Sci Food Saf 2017; 16:632-646. [PMID: 33371562 DOI: 10.1111/1541-4337.12268] [Citation(s) in RCA: 34] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/03/2017] [Revised: 04/04/2017] [Accepted: 04/05/2017] [Indexed: 01/06/2023]
Abstract
Frying is one of the oldest cooking procedures and is still among the most popular ones for food preparation. Due to their unique sensory characteristics, fried foods are consumed often and with pleasure. During frying, part of the oil is absorbed by the food, thereby becoming part of our diet; most interestingly, in the Mediterranean area approximately 50% of total fat intake is provided by cooking fats. Olive oil is the key lipid component of the Mediterranean diet, the health-promoting effects of which have been largely attributed to olive oil intake. Olive oil is unique among vegetable oils due to its desirable lipid profile and some of its minor components. Scientific evidence now indicates that during frying olive oil behavior is usually equal or superior to that of refined vegetable oils. Herein, an overview of virgin olive oil performance under frying is given, with special reference to the fate of olive oil microconstituents. The compositional changes of foods fried in olive oil are also reviewed and discussed in detail.
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Affiliation(s)
- Antonia Chiou
- Laboratory of Chemistry - Biochemistry - Physical Chemistry of Foods, Dept. of Dietetics and Nutrition, Harokopio Univ., 70 El. Venizelou Ave., Kallithea, 176 71, Athens, Greece
| | - Nick Kalogeropoulos
- Laboratory of Chemistry - Biochemistry - Physical Chemistry of Foods, Dept. of Dietetics and Nutrition, Harokopio Univ., 70 El. Venizelou Ave., Kallithea, 176 71, Athens, Greece
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32
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Seçmeler Ö, Güçlü Üstündağ Ö. Behavior of lipophilic bioactives during olive oil processing. EUR J LIPID SCI TECH 2017. [DOI: 10.1002/ejlt.201600404] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/11/2022]
Affiliation(s)
- Özge Seçmeler
- Faculty of Engineering; Department of Food Engineering; Yeditepe University; Istanbul Turkey
| | - Özlem Güçlü Üstündağ
- Faculty of Engineering; Department of Food Engineering; Yeditepe University; Istanbul Turkey
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33
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Reboredo-Rodríguez P, González-Barreiro C, Cancho-Grande B, Valli E, Bendini A, Gallina Toschi T, Simal-Gandara J. Characterization of virgin olive oils produced with autochthonous Galician varieties. Food Chem 2016; 212:162-71. [PMID: 27374520 DOI: 10.1016/j.foodchem.2016.05.135] [Citation(s) in RCA: 27] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/17/2015] [Revised: 05/12/2016] [Accepted: 05/20/2016] [Indexed: 11/29/2022]
Abstract
The interest of Galician oil producers (NW Spain) in recovering the ancient autochthonous olive varieties Brava and Mansa has increased substantially in recent years. Virgin olive oils produced by co-crushing both varieties in two different proportions, reflecting the usual and most common practice adopted in this region, have gradually emerged for the production of virgin olive oils. Herein, the sensory and chemical characteristics of such oils were characterized by quality and genuineness-related parameters. The results of chemical analysis are discussed in terms of their effective contribution to the sensory profile, which suggests useful recommendations for olive oil producers to improve the quality of oils. Antioxidant compounds, together with aromas and coloured pigments were determined, and their contribution in determining the functional value and the sensory properties of oils was investigated. In general, given the high levels of phenolic compounds (ranging between 254 and 375mg/kg oil), tocopherols (about 165mg/kg oil) and carotenoids (10-12mg/kg oil); these are oils with long stability, especially under dark storage conditions, because stability is reinforced with the contribution of chlorophylls (15-22mg/kg oil). A major content of phenolic compounds, as well as a predominance of trans-2-hexen-1-al within odor-active compounds (from 897 to 1645μg/kg oil), responsible for bitter sensory notes. This characterization allows to developing new antioxidant-rich and flavour-rich VOOs, when co-crushing with a higher proportion of Brava olives, satisfying the consumers' demand in having access to more healthy dishes and peculiar sensory attributes.
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Affiliation(s)
- Patricia Reboredo-Rodríguez
- Analytical and Food Chemistry Department, Nutrition and Bromatology Group, University of Vigo, Ourense, Spain
| | - Carmen González-Barreiro
- Analytical and Food Chemistry Department, Nutrition and Bromatology Group, University of Vigo, Ourense, Spain
| | - Beatriz Cancho-Grande
- Analytical and Food Chemistry Department, Nutrition and Bromatology Group, University of Vigo, Ourense, Spain
| | - Enrico Valli
- Department of Agricultural and Food Sciences, Alma Mater Studiorum-University of Bologna, Cesena (FC), Italy
| | - Alessandra Bendini
- Department of Agricultural and Food Sciences, Alma Mater Studiorum-University of Bologna, Cesena (FC), Italy; Interdepartmental Centre for Agri-Food Industrial Research, Alma Mater Studiorum-University of Bologna, Cesena (FC), Italy
| | - Tullia Gallina Toschi
- Department of Agricultural and Food Sciences, Alma Mater Studiorum-University of Bologna, Cesena (FC), Italy; Interdepartmental Centre for Agri-Food Industrial Research, Alma Mater Studiorum-University of Bologna, Cesena (FC), Italy
| | - Jesus Simal-Gandara
- Analytical and Food Chemistry Department, Nutrition and Bromatology Group, University of Vigo, Ourense, Spain.
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34
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Ravi Kumar S, Yamauchi I, Narayan B, Katsuki A, Hosokawa M, Miyashita K. Squalene modulates fatty acid metabolism: Enhanced EPA/DHA in obese/diabetic mice (
KK‐A
y
) model. EUR J LIPID SCI TECH 2016. [DOI: 10.1002/ejlt.201600006] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/11/2022]
Affiliation(s)
| | - Ippei Yamauchi
- Faculty of Fisheries SciencesHokkaido UniversityHakodateHokkaidoJapan
| | - Bhaskar Narayan
- Faculty of Fisheries SciencesHokkaido UniversityHakodateHokkaidoJapan
- Department of Meat and Marine SciencesCSIR‐Central Food Technological Research Institute (CSIR‐CFTRI)MysoreKarnatakaIndia
| | - Ami Katsuki
- Faculty of Fisheries SciencesHokkaido UniversityHakodateHokkaidoJapan
| | - Masashi Hosokawa
- Faculty of Fisheries SciencesHokkaido UniversityHakodateHokkaidoJapan
| | - Kazuo Miyashita
- Faculty of Fisheries SciencesHokkaido UniversityHakodateHokkaidoJapan
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35
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Beltrán G, Bucheli ME, Aguilera MP, Belaj A, Jimenez A. Squalene in virgin olive oil: Screening of variability in olive cultivars. EUR J LIPID SCI TECH 2015. [DOI: 10.1002/ejlt.201500295] [Citation(s) in RCA: 48] [Impact Index Per Article: 4.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/09/2022]
Affiliation(s)
- Gabriel Beltrán
- IFAPA Center Venta del Llano; Junta de Andalucia Mengibar Jaén Spain
| | - Maria E. Bucheli
- IFAPA Center Venta del Llano; Junta de Andalucia Mengibar Jaén Spain
| | - Maria P. Aguilera
- IFAPA Center Venta del Llano; Junta de Andalucia Mengibar Jaén Spain
| | - Angjelina Belaj
- IFAPA Center Alameda del Obispo; Junta de Andalucia, Avda. Menendez Pidal s/n Cordoba Spain
| | - Antonio Jimenez
- IFAPA Center Venta del Llano; Junta de Andalucia Mengibar Jaén Spain
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36
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Song X, Wang X, Tan Y, Feng Y, Li W, Cui Q. High Production of Squalene Using a Newly Isolated Yeast-like Strain Pseudozyma sp. SD301. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2015; 63:8445-51. [PMID: 26350291 DOI: 10.1021/acs.jafc.5b03539] [Citation(s) in RCA: 19] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/23/2023]
Abstract
A yeast-like fungus, termed strain SD301, with the ability to produce a high concentration of squalene, was isolated from Shuidong Bay, China. The nucleotide sequence analysis of the internal transcribed spacer (ITS) region of SD301 indicated the strain belonged to Pseudozyma species. The highest biomass and squalene production of SD301 were obtained when glucose and yeast extracts were used as the carbon and nitrogen sources, respectively, with a C/N ratio of 3. The optimal pH and temperature were 6 and 25 °C, with 15 g L(-1) of supplemented sea salt. The maximum squalene productivity reached 0.039 g L(-1) h(-1) in batch fermentation, while the maximum squalene yield of 2.445 g L(-1) was obtained in fed-batch fermentation. According to our knowledge, this is the highest squalene yield produced thus far using fermentation technology, and the newly isolated strain Pseudozyma sp. SD301 is a promising candidate for commercial squalene production.
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Affiliation(s)
| | - Xiaolong Wang
- Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China , 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China
| | | | | | - Wenli Li
- Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China , 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China
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37
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Hamadate N, Matsumoto Y, Seto K, Yamamoto T, Yamaguchi H, Nakagawa T, Yamamoto E, Fukagawa M, Yazawa K. Vascular effects and safety of supplementation with shark liver oil in middle-aged and elderly males. Exp Ther Med 2015; 10:641-646. [PMID: 26622368 DOI: 10.3892/etm.2015.2568] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/12/2014] [Accepted: 01/22/2015] [Indexed: 12/16/2022] Open
Abstract
Shark liver oil (SLO) has long been used as a traditional health food, with a particular benefit for vascular health, in Japan. The aim of this study was to assess the effect of dietary supplementation with SLO on arterial stiffness and peripheral microvascular function in otherwise healthy middle-aged and older males with slightly increased arterial stiffness. A randomized, double-blind, placebo-controlled, parallel study design was used to assign 41 healthy males with a mean age of 59.0±4.0 years (range, 45-69 years) to either SLO (n=21) or placebo (n=20) treatment for eight weeks. The effects on arterial stiffness and peripheral microvascular function were assessed by the cardio-ankle vascular index (CAVI) and by measurement of hand blood flow to cutaneous tissues using a laser Doppler perfusion imaging (LDPI) technique, respectively. Although the magnitude of the changes in the CAVI value during the eight-week intervention for the SLO group did not significantly differ from that for the placebo group, the changes in the CAVI value for the former group were significantly associated (r=0.575, P<0.01) with age. It was also found that the LDPI values at week 8 were significantly lowered (P<0.05) compared with the baseline values in the placebo group, while no change was observed in the SLO group, resulting in a significant difference in the changes between the two groups (P=0.002). Neither SLO supplementation-related adverse side-effects nor any abnormal changes in routine laboratory tests, including lipid profiles and anthropometric and haemodynamic parameters, were observed throughout the intervention. SLO may have the potential to safely improve vascular health in middle-aged and elderly males.
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Affiliation(s)
- Naobumi Hamadate
- Department of Research and Development, Egao Co., Ltd., Kumamoto 862-0902, Japan
| | - Yoshiyuki Matsumoto
- Department of Research and Development, Egao Co., Ltd., Kumamoto 862-0902, Japan
| | - Kayoko Seto
- Department of Research and Development, Egao Co., Ltd., Kumamoto 862-0902, Japan
| | - Tetsuro Yamamoto
- Research Center, TTC Co., Ltd., Shibuya-ku, Tokyo 113-0021, Japan
| | - Hideyo Yamaguchi
- Research Center, TTC Co., Ltd., Shibuya-ku, Tokyo 113-0021, Japan
| | - Takashi Nakagawa
- Research Center, TTC Co., Ltd., Shibuya-ku, Tokyo 113-0021, Japan
| | - Etsushi Yamamoto
- Research Center, TTC Co., Ltd., Shibuya-ku, Tokyo 113-0021, Japan
| | - Mitsuhiko Fukagawa
- Shinyokohama Shinoharaguchi Orthopaedic Surgery/Dermatology Clinic, Yokohama, Kanagawa 222-0026, Japan
| | - Kazunaga Yazawa
- Laboratory of Nutraceuticals and Functional Foods Science, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo 108-8477, Japan
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38
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Kalogeropoulos N, Tsimidou MZ. Antioxidants in Greek Virgin Olive Oils. Antioxidants (Basel) 2014; 3:387-413. [PMID: 26784878 PMCID: PMC4665486 DOI: 10.3390/antiox3020387] [Citation(s) in RCA: 84] [Impact Index Per Article: 7.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/20/2014] [Revised: 03/31/2014] [Accepted: 04/10/2014] [Indexed: 01/10/2023] Open
Abstract
Greece is ranked third after Spain and Italy in virgin olive oil production. The number of Greek olive cultivars-excluding clonal selections-is greater than 40; however, more than 90% of the acreage is cultivated with 20 cultivars, adapted to a wide range of environmental conditions. Greek virgin olive oils, produced mainly with traditional, non-intensive cultivation practices, are mostly of exceptional quality. The benefits of consuming virgin olive oil, originally attributed to its high oleic acid content, are now considered to be the combined result of several nutrient and non-nutrient phytochemicals. The present work summarizes available data regarding natural antioxidants in Greek virgin olive oils (VOO) namely, polar phenolic compounds, tocopherols, squalene, and triterpenic acids. The literature survey indicated gaps in information, which should be filled in the near future so that the intrinsic properties of this major agricultural product of Greece will be substantiated on a solid scientific basis.
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Affiliation(s)
- Nick Kalogeropoulos
- Laboratory of Chemistry, Biochemistry, Physical Chemistry of Foods, Department of Nutrition and Dietetics, School of Health Science and Education, Harokopio University, 70 El. Venizelou Str., Athens 17661, Greece.
| | - Maria Z Tsimidou
- Laboratory of Food Chemistry and Technology, School of Chemistry, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece.
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39
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Walker WT, Faust SN. Monovalent inactivated split-virion AS03-adjuvanted pandemic influenza A (H1N1) vaccine. Expert Rev Vaccines 2014; 9:1385-98. [DOI: 10.1586/erv.10.141] [Citation(s) in RCA: 29] [Impact Index Per Article: 2.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/08/2022]
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40
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Hull CM, Loveridge EJ, Rolley NJ, Donnison IS, Kelly SL, Kelly DE. Co-production of ethanol and squalene using a Saccharomyces cerevisiae ERG1 (squalene epoxidase) mutant and agro-industrial feedstock. BIOTECHNOLOGY FOR BIOFUELS 2014; 7:133. [PMID: 25298782 PMCID: PMC4189534 DOI: 10.1186/s13068-014-0133-7] [Citation(s) in RCA: 21] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 01/29/2014] [Accepted: 08/29/2014] [Indexed: 05/23/2023]
Abstract
BACKGROUND Genetically customised Saccharomyces cerevisiae that can produce ethanol and additional bio-based chemicals from sustainable agro-industrial feedstocks (for example, residual plant biomass) are of major interest to the biofuel industry. We investigated the microbial biorefinery concept of ethanol and squalene co-production using S. cerevisiae (strain YUG37-ERG1) wherein ERG1 (squalene epoxidase) transcription is under the control of a doxycycline-repressible tet0 7 -CYC1 promoter. The production of ethanol and squalene by YUG37-ERG1 grown using agriculturally sourced grass juice supplemented with doxycycline was assessed. RESULTS Use of the tet0 7 -CYC1 promoter permitted regulation of ERG1 expression and squalene accumulation in YUG37-ERG1, allowing us to circumvent the lethal growth phenotype seen when ERG1 is disrupted completely. In experiments using grass juice feedstock supplemented with 0 to 50 μg doxycycline mL(-1), YUG37-ERG1 fermented ethanol (22.5 [±0.5] mg mL(-1)) and accumulated the highest squalene content (7.89 ± 0.25 mg g(-1) dry biomass) and yield (18.0 ± 4.18 mg squalene L(-1)) with supplements of 5.0 and 0.025 μg doxycycline mL(-1), respectively. Grass juice was found to be rich in water-soluble carbohydrates (61.1 [±3.6] mg sugars mL(-1)) and provided excellent feedstock for growth and fermentation studies using YUG37-ERG1. CONCLUSION Residual plant biomass components from crop production and rotation systems represent possible substrates for microbial fermentation of biofuels and bio-based compounds. This study is the first to utilise S. cerevisiae for the co-production of ethanol and squalene from grass juice. Our findings underscore the value of the biorefinery approach and demonstrate the potential to integrate microbial bioprocess engineering with existing agriculture.
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Affiliation(s)
- Claire M Hull
- />Institute of Life Science, College of Medicine, Swansea University, Swansea, Wales SA2 8PP UK
| | - E Joel Loveridge
- />School of Chemistry, Cardiff University, Cardiff, Wales CF10 3AT UK
| | - Nicola J Rolley
- />Institute of Life Science, College of Medicine, Swansea University, Swansea, Wales SA2 8PP UK
| | - Iain S Donnison
- />Institute of Biological, Environmental & Rural Sciences, Aberystwyth University, Gogerddan, Aberystwyth, Wales SY23 3EE UK
| | - Steven L Kelly
- />Institute of Life Science, College of Medicine, Swansea University, Swansea, Wales SA2 8PP UK
| | - Diane E Kelly
- />Institute of Life Science, College of Medicine, Swansea University, Swansea, Wales SA2 8PP UK
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41
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Notarnicola M, Caruso MG, Tafaro A, Tutino V, Bianco G, Minoia M, Francavilla A. Dietary-suppression of hepatic lipogenic enzyme expression in intact male transgenic mice. World J Gastroenterol 2013; 19:8671-8677. [PMID: 24379585 PMCID: PMC3870513 DOI: 10.3748/wjg.v19.i46.8671] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 03/18/2013] [Revised: 04/16/2013] [Accepted: 05/08/2013] [Indexed: 02/06/2023] Open
Abstract
AIM: To study, in intact male transgenic mice, the effects of three diets based on olive oil and olive oil diet supplemented with lovastatin and orlistat on hepatic lipogenic enzymes expression, considered markers of cell proliferation.
METHODS: Forty ApcMin/+ mice were randomly divided into 4 groups and fed for 10 wk: olive oil (OO) group, n = 10 animals received a diet with olive oil 12%; olive oil plus lovastatin (LOVA) group, n = 10 animals received the same diet with olive oil supplemented with lovastatin 5 mg/kg; olive oil plus orlistat (OR) group, n = 10 animals fed the diet with olive oil supplemented with orlistat 50 mg/kg and SD group, n = 10 animals fed a standard diet. The activity of lipogenic enzymes and their gene expression were evaluated by radiometric and real-time reverse transcription-polymerase chain reaction assay, respectively.
RESULTS: After 10 wk of dietary treatment, the body weight was no different among animal groups (21.3 ± 3.1 g for standard group, 22.1 ± 3.6 g for OO group, 22.0 ± 3.2 g for LOVA group and 20.7 ± 3.4 g for OR group, data expressed as mean ± SD), observing a generalized well-being in all animals. All the dietary managed treated groups presented significantly reduced hepatic levels of fatty acid synthase, farnesyl pyrophosphate synthase and 3-hydroxyl-3-methyl-glutaryl CoA reductase activity and gene expression when compared with the mice fed the standard diet. To evaluate cell proliferation in the liver of treated mice, the levels of cyclin E mRNA have been measured, demonstrating a significant reduction of cyclin E gene expression in all treated groups. Evidence of reduced hepatic cell proliferation was present overall in OO group mice.
CONCLUSION: We confirm the role of lipogenic enzymes as markers of cell proliferation, suggesting that appropriate dietary management alone or with drugs can be a feasible approach to counteract hepatic cell proliferation in mice.
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Flynn MM, Reinert SE. Comparing an olive oil-enriched diet to a standard lower-fat diet for weight loss in breast cancer survivors: a pilot study. J Womens Health (Larchmt) 2012; 19:1155-61. [PMID: 20545561 DOI: 10.1089/jwh.2009.1759] [Citation(s) in RCA: 22] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/12/2022] Open
Abstract
BACKGROUND Traditional diets that include moderate to high intakes of extra virgin olive oil have been related to a decrease in breast cancer risk. We hypothesized that an olive oil-enriched diet would lead to greater weight loss and acceptance, compared with a standard diet, in women previously diagnosed with invasive breast cancer. METHODS Participants consumed a National Cancer Institute (NCI) diet (total fat > 15% and < 30%) and a plant-based olive oil diet (PBOO; > or = 3 tablespoons of olive oil/day) for 8 weeks, each with random assignment to the order. We established a weight loss goal of at least 5% of baseline weight. After completion of the two diet trials, each participant self-selected one of the diets for an additional 6 months of follow-up for weight management. Body measures were done before and after each diet and after follow-up; fasting blood samples were collected after each diet and after follow-up. RESULTS Forty-four overweight women started and 28 completed the 44-week protocol. Twelve (80%) of the 15 women who started with the PBOO diet achieved a weight loss of > or = 5% compared to 4 (31%) of the 13 who started with the NCI diet (p < 0.01). Nineteen of the 22 women eligible for follow-up chose the PBOO diet, and all completed the study. Of the 3 women who chose the NCI diet for follow-up, 1 completed the study. The PBOO diet resulted in lower triglycerides (NCI 105 +/- 46 mg/dL, PBOO 96 +/- 37 mg/dL, p = 0.06) and higher high-density lipoprotein cholesterol (HDL-C) (NCI 64 +/- 13 mg/dL, PBOO 68 +/- 12 mg/dL, p = 0.001). CONCLUSIONS An olive oil-enriched diet brought about greater weight loss than a lower-fat diet in an 8-week comparison. Moreover, these women chose, overwhelmingly, the olive oil-enriched diet for 6 months of follow-up. An olive oil-enriched diet may be more efficacious for weight loss in breast cancer survivors than a standard lower-fat diet.
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Affiliation(s)
- Mary M Flynn
- The Miriam Hospital and Brown University, Providence, Rhode Island 02906, USA.
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Xu Z, Harvey KA, Pavlina T, Dutot G, Hise M, Zaloga GP, Siddiqui RA. Steroidal compounds in commercial parenteral lipid emulsions. Nutrients 2012; 4:904-21. [PMID: 23016123 PMCID: PMC3448078 DOI: 10.3390/nu4080904] [Citation(s) in RCA: 49] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/14/2012] [Revised: 08/01/2012] [Accepted: 08/01/2012] [Indexed: 01/11/2023] Open
Abstract
Parenteral nutrition lipid emulsions made from various plant oils contain steroidal compounds, called phytosterols. During parenteral administration of lipid emulsions, phytosterols can reach levels in the blood that are many fold higher than during enteral administration. The elevated phytosterol levels have been associated with the development of liver dysfunction and the rare development of liver failure. There is limited information available in the literature related to phytosterol concentrations in lipid emulsions. The objective of the current study was to validate an assay for steroidal compounds found in lipid emulsions and to compare their concentrations in the most commonly used parenteral nutrition lipid emulsions: Liposyn(®) II, Liposyn(®) III, Lipofundin(®) MCT, Lipofundin(®) N, Structolipid(®), Intralipid(®), Ivelip(®) and ClinOleic(®). Our data demonstrates that concentrations of the various steroidal compounds varied greatly between the eight lipid emulsions, with the olive oil-based lipid emulsion containing the lowest levels of phytosterols and cholesterol, and the highest concentration of squalene. The clinical impression of greater incidences of liver dysfunction with soybean versus MCT/LCT and olive/soy lipid emulsions may be reflective of the levels of phytosterols in these emulsions. This information may help guide future studies and clinical care of patients with lipid emulsion-associated liver dysfunction.
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Affiliation(s)
- Zhidong Xu
- Methodist Research Institute, Indiana University Health, Indianapolis, IN 46202, USA; (Z.X.); (K.A.H.)
| | - Kevin A. Harvey
- Methodist Research Institute, Indiana University Health, Indianapolis, IN 46202, USA; (Z.X.); (K.A.H.)
| | - Thomas Pavlina
- Baxter Healthcare Corporation, Deerfield, IL 60015, USA; (T.P.); (M.H.); (G.P.Z.)
| | - Guy Dutot
- Baxter SAS, Maurepas-Cedex 78311, France;
| | - Mary Hise
- Baxter Healthcare Corporation, Deerfield, IL 60015, USA; (T.P.); (M.H.); (G.P.Z.)
| | - Gary P. Zaloga
- Baxter Healthcare Corporation, Deerfield, IL 60015, USA; (T.P.); (M.H.); (G.P.Z.)
| | - Rafat A. Siddiqui
- Methodist Research Institute, Indiana University Health, Indianapolis, IN 46202, USA; (Z.X.); (K.A.H.)
- Department of Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USA
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Abstract
OBJECTIVE To place the Mediterranean diet (MedDi) in the context of the cultural history of the Middle East and emphasise the health effects of some of the biblical seven species - wheat, barley, grapes, figs, pomegranates, olives and date honey. DESIGN Review of the literature concerning the benefits of these foods. SETTING Middle East and Mediterranean Basin. SUBJECTS Mediterranean populations and clinical studies utilising the MedDi. RESULTS AND CONCLUSIONS The MedDi has been associated with lower rates of CVD, and epidemiological evidence promotes the benefits of consuming fruit and vegetables. Recommended foods for optimal health include whole grain, fish, wine, pomegranates, figs, walnuts and extra virgin olive oil. The biblical traditional diet, including the seven species and additional Mediterranean fruits, has great health advantages, especially for CVD. In addition to the diet, lifestyle adaptation that involves increasing physical activity and organised meals, together with healthy food choices, is consistent with the traditional MedDi. The MedDi is a manageable, lifestyle-friendly diet that, when fortified with its biblical antecedent attributes, may prove to be even more enjoyable and considerably healthier in combating the obesogenic environment and in decreasing the risks of the non-communicable diseases of modern life than conventional, modern dietary recommendations. The biblical seven species, together with other indigenous foods from the Middle East, are now scientifically recognised as healthy foods, and further improve the many beneficial effects of the MedDi.
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Ghanbari R, Anwar F, Alkharfy KM, Gilani AH, Saari N. Valuable nutrients and functional bioactives in different parts of olive (Olea europaea L.)-a review. Int J Mol Sci 2012; 13:3291-3340. [PMID: 22489153 PMCID: PMC3317714 DOI: 10.3390/ijms13033291] [Citation(s) in RCA: 303] [Impact Index Per Article: 23.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/21/2011] [Revised: 02/13/2012] [Accepted: 02/16/2012] [Indexed: 02/05/2023] Open
Abstract
The Olive tree (Olea europaea L.), a native of the Mediterranean basin and parts of Asia, is now widely cultivated in many other parts of the world for production of olive oil and table olives. Olive is a rich source of valuable nutrients and bioactives of medicinal and therapeutic interest. Olive fruit contains appreciable concentration, 1-3% of fresh pulp weight, of hydrophilic (phenolic acids, phenolic alchohols, flavonoids and secoiridoids) and lipophilic (cresols) phenolic compounds that are known to possess multiple biological activities such as antioxidant, anticarcinogenic, antiinflammatory, antimicrobial, antihypertensive, antidyslipidemic, cardiotonic, laxative, and antiplatelet. Other important compounds present in olive fruit are pectin, organic acids, and pigments. Virgin olive oil (VOO), extracted mechanically from the fruit, is also very popular for its nutritive and health-promoting potential, especially against cardiovascular disorders due to the presence of high levels of monounsaturates and other valuable minor components such as phenolics, phytosterols, tocopherols, carotenoids, chlorophyll and squalene. The cultivar, area of production, harvest time, and the processing techniques employed are some of the factors shown to influence the composition of olive fruit and olive oil. This review focuses comprehensively on the nutrients and high-value bioactives profile as well as medicinal and functional aspects of different parts of olives and its byproducts. Various factors affecting the composition of this food commodity of medicinal value are also discussed.
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Affiliation(s)
- Rahele Ghanbari
- Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; E-Mail:
| | - Farooq Anwar
- Department of Chemistry, University of Sargodha, Sargodha-40100, Pakistan
| | - Khalid M. Alkharfy
- Department of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia; E-Mail:
| | - Anwarul-Hassan Gilani
- Department of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia; E-Mail:
- Natural Products Research Division, Department of Biologicaland Biomedical Sciences, Aga Khan University Medical College, Karachi 74800, Pakistan; E-Mail:
| | - Nazamid Saari
- Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; E-Mail:
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A quantum chemical study on the free radical scavenging activity of tyrosol and hydroxytyrosol. Theor Chem Acc 2012. [DOI: 10.1007/s00214-012-1173-3] [Citation(s) in RCA: 33] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/10/2023]
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Spanova M, Daum G. Squalene - biochemistry, molecular biology, process biotechnology, and applications. EUR J LIPID SCI TECH 2011. [DOI: 10.1002/ejlt.201100203] [Citation(s) in RCA: 155] [Impact Index Per Article: 11.1] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/25/2022]
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Chen G, Fan KW, Lu FP, Li Q, Aki T, Chen F, Jiang Y. Optimization of nitrogen source for enhanced production of squalene from thraustochytrid Aurantiochytrium sp. N Biotechnol 2010; 27:382-9. [PMID: 20412873 DOI: 10.1016/j.nbt.2010.04.005] [Citation(s) in RCA: 61] [Impact Index Per Article: 4.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/21/2010] [Revised: 03/26/2010] [Accepted: 04/13/2010] [Indexed: 11/28/2022]
Abstract
Nitrogen (N) sources, the critical medium component, were optimized for squalene production by microalga Aurantiochytrium sp. in heterotrophic cultures. In screening experiments monosodium glutamate, yeast extract and tryptone were found to enhance cell growth and squalene production. The optimal levels of the three nitrogen sources were further determined through central composite experimental design. The squalene content and yield were both influenced not only by monosodium glutamate, tryptone and yeast extract, but also by their interactions. The squalene content and squalene yield were described by the second-order polynomial equations with high confidence levels (>99%). The optimal concentrations of monosodium glutamate, yeast extract and tryptone were predicted to be 6.61 g/L, 6.13 g/L and 4.50 g/L for squalene content and 6.94 g/L, 6.22 g/L and 4.40 g/L for squalene yield, respectively. In the verification experiment, the squalene content and squalene yield reached 0.72 mg/g and 5.90 mg/L, respectively, which were much higher than those obtained in previous studies.
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Affiliation(s)
- Guanqun Chen
- Department of Biology and Kwong Living Trust Food Safety & Analysis Laboratory, Hong Kong Baptist University, Kowloon Tong, Hong Kong
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Enhanced production of squalene in the thraustochytrid Aurantiochytrium mangrovei by medium optimization and treatment with terbinafine. World J Microbiol Biotechnol 2010; 26:1303-9. [PMID: 24026934 DOI: 10.1007/s11274-009-0301-2] [Citation(s) in RCA: 52] [Impact Index Per Article: 3.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/17/2009] [Accepted: 12/30/2009] [Indexed: 10/20/2022]
Abstract
Squalene is an effective chemopreventive agent in reducing the incident of coronary heart disease and cancer. It is also a strong antioxidant used extensively in the food and cosmetic industries. Microbial sources of squalene are being explored in recent years. The objective of this study is to increase the squalene content and yield in the thraustochytrid, Aurantiochytrium mangrovei FB3 through medium optimization and the treatment with terbinafine, an inhibitor of squalene monooxygenase in the sterol biosynthetic pathway. The highest biomass concentration of 21.2 g l(-1) was obtained at a glucose concentration of 60 g l(-1), while the highest specific growth rate of 0.077 h(-1) and the growth yield coefficient of 0.44 g g(-1) based on glucose were achieved at a lower glucose concentration (30 g l(-1)). The addition of terbinafine led to a slight inhibition of cell growth whereas an obvious increase in squalene content was observed at terbinafine concentrations of 10 and 100 mg l(-1), which corresponded to an increase of 36 and 40% in squalene content, respectively compared to the control. The addition of terbinafine was thus effective in inducing the accumulation of squalene in A. mangrovei. This study not only demonstrated the production potential of squalene by A. mangrovei, but also provided novel information on the accumulation effect of terbinafine on the biosynthesis of an essential intermediate involved in sterol metabolic pathway.
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