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1
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Hajimohammadi B, Dalimi A, Eslami G, Ahmadian S, Zandi S, Baghbani A, Hosseini SS, Askari V, Sheykhzadegan M, Ardekani MN, Boozhmehrani MJ, Ranjbar MJ, Ghoshouni H, Vakili M. Occurrence and genetic characterization of Echinococcus granulosus sensu lato from domestic animals in Central Iran. BMC Vet Res 2022; 18:22. [PMID: 34996460 PMCID: PMC8740344 DOI: 10.1186/s12917-021-03131-1] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/10/2021] [Accepted: 12/29/2021] [Indexed: 12/19/2022] Open
Abstract
Background The species complex of Echinococcus granulosus sensu lato (s.l.) causes cystic echinococcosis distributed worldwide. There is no genotype information from hydatid cysts in the intermediate hosts in Central Iran. Therefore, in this study, we analyzed the hydatid cysts in livestock slaughtered in an abattoir in this region. Six hundred fifty-seven hydatid cysts were isolated from 97 animals, including sheep, cattle, camels, and goats slaughtered in Yazd abattoir from September 2018 to January 2020. The demographic data was collected as well as cyst location, fertility, and viability. Out of 657 samples, 164 samples were genotyped. Then, phylogenetic analysis was performed using MEGAX. Statistical analyses were done using SPSS version 16.0 by chi-square with a significant difference of less than 0.05. Results Out of 164 samples, the G1-G3 complex genotype had the most frequency in samples, with 135 cases recognized. The G6/G7 was observed in 19 isolates and G5 was reported in nine samples. One sample was detected as Taenia hydatigena. Conclusions This study showed that G1-G3 and G6/G7 genotypes were presented in all animals, but G5 was reported only in cattle, goats, and camels. It is the first molecular identification of cystic echinococcosis in Central Iran. Hence, reporting G5 in livestock in this area should be considered due to transmission to humans.
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Affiliation(s)
- Bahador Hajimohammadi
- Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.,Department of Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
| | - Abdolhossein Dalimi
- Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
| | - Gilda Eslami
- Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
| | - Salman Ahmadian
- Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.,Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
| | - Sajad Zandi
- Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.,Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
| | - Ahmad Baghbani
- Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.,Department of Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
| | - Saeedeh Sadat Hosseini
- Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
| | - Vahideh Askari
- Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
| | - Maryam Sheykhzadegan
- Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
| | | | - Mohammad Javad Boozhmehrani
- Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.,Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
| | - Mohammad Javad Ranjbar
- Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
| | - Hamed Ghoshouni
- Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
| | - Mahmood Vakili
- Department of Community and Preventive Medicine, Health Monitoring Research Center, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
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2
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Khan SN, Ali R, Khan S, Norin S, Rooman M, Akbar NU, Khan TA, Haleem S, Khan MA, Ali I. Cystic echinococcosis: an emerging zoonosis in southern regions of Khyber Pakhtunkhwa, Pakistan. BMC Vet Res 2021; 17:139. [PMID: 33794898 PMCID: PMC8015088 DOI: 10.1186/s12917-021-02830-z] [Citation(s) in RCA: 12] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/11/2020] [Accepted: 03/10/2021] [Indexed: 02/07/2023] Open
Abstract
Background Cystic echinococcosis (CE) is one of the principal causes of economic loss to the livestock industry because of its morbidity and mortality of food-producing animals and condemnation of important visceral organs. Pakistan being an agricultural country having an extensive livestock sector, is mostly practiced by poor people, which has a fundamental role in the economy. The present study was aimed to conduct a cross-sectional survey and PCR based confirmation of Echinococcus granulosus in sheep, goats, cows, and buffaloes from southern regions (three districts: Lakki Marwat, Bannu, and Karak) of Khyber Pakhtunkhwa, Pakistan. During the study, a total of 2833 animals were examined randomly including; sheep (n = 529), goats (n = 428), cows (n = 1693), and buffaloes (n = 183). Hydatid cysts were collected and examined for the presence of protoscoleces using microscopy. Detection of DNA was performed by using PCR and two mitochondrial genetic markers namely; NAD-1 and COX-1 were amplified. Results The overall prevalence of CE was found to be (9%) among the examined animals. The hydatid cyst infection was highly prevalent in buffaloes (12%), followed by sheep (10%), cows (9%), and goats (5.1%). Cystic echinococcosis was more prevalent (10%; 96/992) in district Lakki Marwat followed by district Bannu (9%; 112/1246) and Karak (7%; 39/595). Female animals were more likely to be infected with CE (11.6%) than male animals (5.3%) (p = 0.001). Similarly, the infection was higher in the older group of animals as compared to younger (p = 0.001). Mostly (52.2%; n = 129) of hydatid cysts were found in the liver, while (64.4%; n = 159) cysts of the infected animals were infertile. PCR based identification confirmed the presence of E. granulosus sensu stricto (s.s) in the study area. Conclusion Cystic echinococcosis was found to be highly prevalent in southern regions of Khyber Pakhtunkhwa and could be a potential threat to human health. Moreover, molecular sequencing and phylogenetic analyses should be carried out in future to identify the prevailing genotype (s) of E. granulosus s.s.
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Affiliation(s)
- Shahid Niaz Khan
- Department of Zoology, Kohat University of Science and Technology Kohat-26000, Khyber Pakhtunkhwa, Pakistan.
| | - Rehman Ali
- Department of Zoology, Kohat University of Science and Technology Kohat-26000, Khyber Pakhtunkhwa, Pakistan.
| | - Sanaullah Khan
- Department of Zoology, University of Peshawar, Khyber Pakhtunkhwa, Pakistan
| | - Sadia Norin
- Department of Zoology, Kohat University of Science and Technology Kohat-26000, Khyber Pakhtunkhwa, Pakistan
| | - Muhammad Rooman
- Department of Zoology, Hazara University, Khyber Pakhtunkhwa, Pakistan
| | - Noor Ul Akbar
- Department of Zoology, Kohat University of Science and Technology Kohat-26000, Khyber Pakhtunkhwa, Pakistan
| | - Taj Ali Khan
- Institute of Pathology and Diagnostic Medicine, Khyber Medical University, Peshawar, Pakistan
| | - Sumbal Haleem
- Department of Zoology, Kohat University of Science and Technology Kohat-26000, Khyber Pakhtunkhwa, Pakistan
| | - Murad Ali Khan
- College of Veterinary Sciences, Faculty of Animal Husbandry and Veterinary Sciences, University of Agriculture, Peshawar, Pakistan
| | - Ijaz Ali
- Department of Biosciences, COMSATS University, Islamabad, Pakistan.
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Samiee-Rad F, Emami A. An Iranian man with increased thigh mass due to a hydatid cyst. GMS HYGIENE AND INFECTION CONTROL 2020; 15:Doc20. [PMID: 32884894 PMCID: PMC7453142 DOI: 10.3205/dgkh000355] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Indexed: 11/30/2022]
Abstract
Background: Hydatid cyst is a zoonotic infection caused by Echinococcosis granulosus. Primary single-intramuscular hydatid disease is rare, even in endemic regions of the world. Here we report the case of exceptional thigh mass due to a hydatid cyst in an Iranian man. Case presentation: An 86-year-old man, initially presented to Velayat teaching hospital surgery clinic in May 2017 with a single right-thigh mass, but physical examinations of other organs were unremarkable. Based on sonographic findings, the differential diagnosis was hydatid cyst. He underwent surgical resection of the cyst. Histopathological results confirmed the diagnosis. There was no evidence of recurrence of the lesion during the 23-month follow-up. Conclusion: Increase thigh mass due to a hydatid cyst is a rare event. In endemic regions with the presence of hydatid cysts, especially physicians of surgical clinics have to consider differential diagnosis of hydatid cysts in unusual locations in case of such a lesion.
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Affiliation(s)
- Fatemeh Samiee-Rad
- Department of Pathology, Qazvin University of Medical Sciences, Qazvin, Iran
| | - Ali Emami
- Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran
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Mehmood N, Arshad M, Ahmed H, Simsek S, Muqaddas H. Comprehensive Account on Prevalence and Characteristics of Hydatid Cysts in Livestock from Pakistan. THE KOREAN JOURNAL OF PARASITOLOGY 2020; 58:121-127. [PMID: 32418380 PMCID: PMC7231835 DOI: 10.3347/kjp.2020.58.2.121] [Citation(s) in RCA: 18] [Impact Index Per Article: 3.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 09/16/2019] [Revised: 11/09/2019] [Accepted: 12/23/2019] [Indexed: 12/17/2022]
Abstract
Pakistan is at intersection of hyperendemic regions for hydatidosis. Current study aimed to determine the prevalence of hydatid cysts and cyst characteristics in different intermediate hosts (sheep, goats, cattle and buffaloes) across the 4 provinces of Pakistan. A total of 991 sheep, 1,478 goats, 1,602 cattle and 1,343 buffaloes were examined for presence of hydatid cysts during 2 years (January 2016-December 2018). Differences in frequency of hydatidosis were observed with highest overall prevalence in buffaloes (11.9%) and sheep (11.5%). Highest prevalence and burden of infection were observed in older age animals (23.8%, 9.78±0.49) and females (26.5%, 12.53±0.67). Data for seasonal prevalence alluded to year-round presence of disease with non-significant statistical difference. Organ predilection indicated liver as the most preferred site of cyst localization followed mainly by lungs. An over-dispersion pattern was observed in all infected animals as majority of cysts belonged <10 cysts per infected host category. Highest percentage of fertile cysts was observed in liver of sheep. Interestingly, solitary form of cysts had higher fertility rate than multiple form. Amid lack of data and wide gap of knowledge, this study would try to fill up the lacunae regarding this neglected tropical disease. Extensive rearing of livestock, unregulated official slaughter and home slaughtering have played role in adaptability of E. granulosus in Pakistan.
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Affiliation(s)
- Naunain Mehmood
- Department of Zoology, University of Sargodha, Sargodha, Pakistan
| | - Muhammad Arshad
- Department of Zoology, University of Sargodha, Sargodha, Pakistan
| | - Haroon Ahmed
- Department of Biosciences, COMSATS University Islamabad, Islamabad, Pakistan
| | - Sami Simsek
- Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119 Elazig, Turkey
| | - Hira Muqaddas
- Department of Zoology, University of Sargodha, Sargodha, Pakistan
- Department of Zoology, Women University Multan, Multan, Pakistan
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Khaled K, Teber G, Bouaicha F, Amairia S, Rekik M, Gharbi M. Infestation of small ruminants by the metacestode stage of Taenia hydatigena in slaughterhouse, North East Tunisia. Vet Med Sci 2019; 6:204-208. [PMID: 31782917 PMCID: PMC7196678 DOI: 10.1002/vms3.222] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/03/2019] [Revised: 09/21/2019] [Accepted: 10/19/2019] [Indexed: 11/25/2022] Open
Abstract
Background Cysticercus tenuicollis (larvae of Taenia hydatigena) is a frequent cosmopolitan endoparasite of ruminants. The infestation by this parasite is underestimated since it is neither zoonotic nor inducing high economic losses in the sheep sector. Methods This study aimed at estimating different parasitological indicators, Cysticercus tenuicollis infestation in small ruminants using a slaughterhouse‐based survey in Northeast Tunisia. A total number of 3,692 sheep and 78 goats were examined in the slaughterhouse of Menzel Temime. Results The overall prevalence was estimated to be 2.8 (106/3692) and 8.9% (7/78) in sheep and goats, respectively. The abundance in these two species was 0.24 and 0.05 and the intensity 1.97 and 2.85, respectively. In goats, all the cysts were found in the mesentery, whilst, in sheep, the majority were in mesentery (96%) but also on the liver in 2% of the cases and in both organs (2%). There were only cysts with aqueous liquid with predominantly middle‐sized cysts (1 to 3 cm) corresponding to 63.15 and 70.34% in sheep and goats, respectively. Conclusion Since, in small ruminants, the infestation by C. tenuicollis indicators are not high, low cost control measures should be implemented to eliminate this parasite in Northeast Tunisia.
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Affiliation(s)
- Khouloud Khaled
- Laboratoire de Parasitologie, Institution de la Recherche et de l'Enseignement Supérieur Agricoles & Univ. Manouba, École Nationale de Médecine Vétérinaire de Sidi Thabet, Sidi Thabet, Tunisia
| | - Ghaleb Teber
- Municipality of Menzel Temime, Menzel Temime, Tunisia
| | - Faten Bouaicha
- Laboratoire de Parasitologie, Institution de la Recherche et de l'Enseignement Supérieur Agricoles & Univ. Manouba, École Nationale de Médecine Vétérinaire de Sidi Thabet, Sidi Thabet, Tunisia
| | - Safa Amairia
- Laboratoire de Parasitologie, Institution de la Recherche et de l'Enseignement Supérieur Agricoles & Univ. Manouba, École Nationale de Médecine Vétérinaire de Sidi Thabet, Sidi Thabet, Tunisia
| | - Mourad Rekik
- International Center for Agricultural Research in the Dry Areas (ICARDA), Amman, Jordan
| | - Mohamed Gharbi
- Laboratoire de Parasitologie, Institution de la Recherche et de l'Enseignement Supérieur Agricoles & Univ. Manouba, École Nationale de Médecine Vétérinaire de Sidi Thabet, Sidi Thabet, Tunisia
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Miran MB, Kasuku AA, Swai ES. Prevalence of echinococcosis and Taenia hydatigena cysticercosis in slaughtered small ruminants at the livestock-wildlife interface areas of Ngorongoro, Tanzania. Vet World 2017; 10:411-417. [PMID: 28507413 PMCID: PMC5422245 DOI: 10.14202/vetworld.2017.411-417] [Citation(s) in RCA: 15] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/25/2016] [Accepted: 02/27/2017] [Indexed: 12/02/2022] Open
Abstract
Aim: Echinococcosis or hydatidosis (due to the larval stage of Echinococcus spp.) and cysticercosis (due to the larval stage of Taenia hydatigena) pose a significant economic losses due to slaughter condemnation and risk to public health in developing countries such as Tanzania where sanitation is poor and people live in close proximity with each other and with animals. This study was conducted to determine the prevalence of and to identify the predisposing factors for echinococcosis and cysticercosis in sheep and goats at three slaughter slabs located in the livestock-wildlife interface areas of Ngorongoro, Tanzania. Materials and Methods: A cross-sectional based survey was conducted, from January 2013 to April 2013, whereby a total of 180 animals comprising 90 goats and 90 sheep of both sexes were examined at postmortem for the evidence of larval stages of Echinococcus spp. (hydatid cyst) and T. hydatigena (Cysticercus tenuicollis) through visual inspection, incision and palpation of organs and viscera. Results: The prevalence of echinococcosis was 22.2% and 16.6%, in goats and sheep, respectively, while the overall infection rates for cysticercosis were 61.1% in goats and 42.2% in sheep. The result of this study revealed that goats and sheep in Malambo slaughter slab had significantly higher prevalence of T. hydatigena (C. tenuicollis) and hydatid cysts (p<0.05) compared to other slab points. T. hydatigena (C. tenuicollis) cysts were more frequently detected in the omentum than other visceral organs among the animals examined. Conclusion: In conclusion, the observed high prevalence of the two metacestodes larval stages leads to high condemnation rates of edible offals and raises significant public health concerns. This underscores for the need to undertake more extensive epidemiological investigations to better determine the causal factors, economic impact, and public health importance of the disease in this livestock-wildlife interface setting.
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Affiliation(s)
- M B Miran
- Department of Livestock, Ngorongoro District Council, P. O. Box 1, Loliondo, Tanzania
| | - A A Kasuku
- Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, Sokoine University of Agriculture, P.O. Box 3015, Morogoro, Tanzania
| | - E S Swai
- Ministry of Agriculture, Livestock and Fisheries, P. O. Box 9152, Dar-es-Salaam, Tanzania
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7
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Lahmar S, Boufana B, Jebabli L, Craig PS, Ayari H, Basti T, Dhibi M, Torgerson PR. Modelling the transmission dynamics of cystic echinococcosis in donkeys of different ages from Tunisia. Vet Parasitol 2014; 205:119-24. [DOI: 10.1016/j.vetpar.2014.06.007] [Citation(s) in RCA: 7] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/20/2013] [Revised: 06/03/2014] [Accepted: 06/06/2014] [Indexed: 11/30/2022]
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Borji H, Parandeh S. The abattoir condemnation of meat because of parasitic infection, and its economic importance: results of a retrospective study in north–eastern Iran. ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY 2013; 104:641-7. [DOI: 10.1179/136485910x12851868780261] [Citation(s) in RCA: 29] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/28/2022]
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9
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Khan AM, Gazi M, Bashir S. Seasonal prevalence of hydatidosis in buffaloes –A retrospective study. Vet World 2013. [DOI: 10.14202/vetworld.2013.647-650] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/16/2022] Open
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10
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Borji H, Azizzadeh M, Kamelli M. A Retrospective Study of Abattoir Condemnation Due to Parasitic Infections: Economic Importance in Ahwaz, Southwestern Iran. J Parasitol 2012; 98:954-7. [DOI: 10.1645/ge-2988.1] [Citation(s) in RCA: 35] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/10/2022] Open
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11
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Grosso G, Gruttadauria S, Biondi A, Marventano S, Mistretta A. Worldwide epidemiology of liver hydatidosis including the Mediterranean area. World J Gastroenterol 2012; 18:1425-37. [PMID: 22509074 PMCID: PMC3319938 DOI: 10.3748/wjg.v18.i13.1425] [Citation(s) in RCA: 195] [Impact Index Per Article: 15.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 07/02/2011] [Revised: 09/17/2011] [Accepted: 10/14/2011] [Indexed: 02/06/2023] Open
Abstract
The worldwide incidence and prevalence of cystic echinococcosis have fallen dramatically over the past several decades. Nonetheless, infection with Echinococcus granulosus (E. granulosus) remains a major public health issue in several countries and regions, even in places where it was previously at low levels, as a result of a reduction of control programmes due to economic problems and lack of resources. Geographic distribution differs by country and region depending on the presence in that country of large numbers of nomadic or semi-nomadic sheep and goat flocks that represent the intermediate host of the parasite, and their close contact with the final host, the dog, which mostly provides the transmission of infection to humans. The greatest prevalence of cystic echinococcosis in human and animal hosts is found in countries of the temperate zones, including several parts of Eurasia (the Mediterranean regions, southern and central parts of Russia, central Asia, China), Australia, some parts of America (especially South America) and north and east Africa. Echinococcosis is currently considered an endemic zoonotic disease in the Mediterranean region. The most frequent strain associated with human cystic echinococcosis appears to be the common sheep strain (G1). This strain appears to be widely distributed in all continents. The purpose of this review is to examine the distribution of E. granulosus and the epidemiology of a re-emerging disease such as cystic echinococcosis.
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Occurrence of hydatidosis in camels (Camelus dromedarius) and their potential role in the epidemiology of Echinococcus granulosus in Kerman area, southeast of Iran. ACTA ACUST UNITED AC 2011. [DOI: 10.1007/s00580-011-1200-0] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/18/2022]
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Abstract
Sustained research efforts over the last 50 years have revealed a considerable amount of information about immunity to taeniid cestode infections in the parasites’ intermediate hosts. As a product of this research, a series of effective recombinant vaccines have been developed which have no parallel in any other group of parasitic organisms. There are, however, many important aspects relating to immunity that remain to be elucidated. Some concepts have come to be firmly held as facts and yet the supportive data are either conflicting or unconfirmed. This review considers the phenomenon of immunity to re-infection with taeniid cestodes in their intermediate hosts, examining carefully the nature of the evidence that is available to support conclusions that have been drawn in this area.
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Affiliation(s)
- M W Lightowlers
- Faculty of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia.
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14
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Occurrence and financial loss assessment of cystic echinococcosis (hydatidosis) in cattle slaughtered at Wolayita Sodo municipal abattoir, Southern Ethiopia. Trop Anim Health Prod 2010; 43:221-8. [PMID: 20803350 DOI: 10.1007/s11250-010-9680-5] [Citation(s) in RCA: 21] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 08/03/2010] [Indexed: 10/19/2022]
Abstract
A cross-sectional study aimed at determining the prevalence and cyst characteristics and estimating the financial loss due to cystic echinococcosis (hydatidosis) in cattle slaughtered at Wolayita Sodo municipal abattoir was conducted from November 2009 to April 2010. Out of 546 cattle examined, 92 (16.85%) were found to harbor visible hydatid cysts. Significantly higher infection was detected in local (P < 0.05) than crossbred cattle. No significant variation was observed with regard to origin, sex, and body condition status of animals. Regarding organ distribution, infections of the lung, liver, spleen, and kidney were 57.78%, 35.46%, 8.75%, and 4.01%, respectively. Of the total 1,097 hydatid cysts counted, 952 (86.78%), 136 (12.4%), and eight (0.82%) were found to be small-sized, medium-sized, and large-sized, respectively. Likewise, out of 450 cysts assessed, 138 (30.67%) were fertile, 241 (53.56%) sterile, and 71 (15.78%) calcified. Of the 138 fertile cysts subjected for viability test, 13 (9.42%) were viable while 125 (90.57%) were nonviable. Moreover, assessment of annual economic loss due to bovine hydatidosis at Wolayita Sodo municipal abattoir from offal condemnation and carcass weight loss was estimated at 410,755.90 Ethiopian Birr (ETB; 30,202.64 US$; 1 US$ = 13.60 ETB). Despite the moderate magnitude of infection detected currently, there seems to be an existing socioeconomic situation favorable for hydatidosis, and hence, it remains one of the most important diseases warranting serious attention for prevention and control actions in Wolayita zone. Hence, establishment of well-equipped standardized abattoirs, creation of public awareness, and control of stray dogs are of paramount importance.
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Abstract
Echinococcosis/hydatidosis is one of the most important parasitic zoonotic diseases in the world. Both cystic hydatidosis (CE) caused by Echinococcus granulosus and alveolar echinococcosis (AE) caused by E. multilocularis have been reported in several countries of the Mediterranean region (MR). E. granulosus has always been present in the MR and is the most common species. This parasite depends on the dog-sheep cycle and is actively transmitted in all pastoral regions where sheep, cattle and camelids predominate. E. multilocularis occurs only sporadically in limited areas of France, Serbia and Montenegro, Turkey, Tunisia and Morocco. However recent evidence indicates that it is spreading into other regions of the Mediterranean. Due to the lack of well-documented data, and to the fact that CE is not a notifiable disease in the majority of M countries, the precise incidence and prevalence of CE in humans and animals are not known. Published data suggests that prevalence is rather high in North Africa, Turkey, Greece, and in several regions of Italy and Spain. CE is an increasing public health and socio-economic concern due to the considerable morbidity rates that give rise to high economic losses both in the public health sector and in the livestock industry. Hospitalisation for human CE lasts from 2 weeks to more than 1 month in case of surgery. A number of factors contribute to the increase of prevalence and to the spreading of CE in the MR. These include the diversity of livestock production systems (predominantly extensive, traditional animal husbandry), small, ill-equipped and unsupervised slaughter-houses, illegal and family slaughtering, low public awareness of hydatid diseases, and the high population of stray dogs. Cyprus is the only country where an eradication programme has been successfully implemented. There have been, however, important developments in the last decade in CE epidemiology, in the diagnosis of canine infection, in strain characterisation and in immune strategies against CE in animals. This scientific progress, together with effective health education programmes, will likely improve control programmes and reduce the time required to achieve significant decreases in prevalence or eradication.
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Affiliation(s)
- A Dakkak
- Department of Pathology and Veterinary Public Health, Parasitology and Parasitic Diseases Unit, Institut Agronomique et Vétérinaire Hassan II, B.P. 60202, Rabat-Instituts, Morocco.
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16
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A molecular survey of cystic echinococcosis in Sudan. Vet Parasitol 2010; 169:340-6. [DOI: 10.1016/j.vetpar.2010.01.004] [Citation(s) in RCA: 68] [Impact Index Per Article: 4.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/29/2009] [Revised: 12/04/2009] [Accepted: 01/05/2010] [Indexed: 11/24/2022]
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Regassa F, Molla A, Bekele J. Study on the prevalence of cystic hydatidosis and its economic significance in cattle slaughtered at Hawassa Municipal abattoir, Ethiopia. Trop Anim Health Prod 2010; 42:977-84. [PMID: 20111997 DOI: 10.1007/s11250-009-9517-2] [Citation(s) in RCA: 28] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 11/24/2009] [Indexed: 11/24/2022]
Abstract
A cross-sectional study was conducted from December 2008 to March 2009 to assess the status of cystic hydatidosis in cattle slaughtered at Hawassa Municipal abattoir. Out of the total 632 cattle examined visually and manually (palpation and incision), 333 (52.69%) were found harboring hydatid cysts. A significantly higher infection was detected in older cattle (P < 0.05, chi(2) = 4.36) than young. Regarding body condition score, no significant variation (P > 0.05, chi(2) = 2.148) was observed as the prevalence was 54.55% for lean cattle followed by medium (53.83%) and fat (46.88%). Of the total 333 infected, 123 (36.9%) had hydatid cysts only in the lung, 23 (6.9%) in the liver, 12 (3.6%) in the spleen, five (1.5%) in the heart, and three (0.9%) in the kidney while the rest 167 (50.2%) had multiple organ infections. Of the 530 viscera harboring hydatid cysts, the highest (52.83%) was lung followed by liver (34.15%), spleen (9.06%), heart (3.39%), and kidney (0.56%). Size assessment made on 874 cysts indicated that 308 (35.3%) were small, 251 (28.7%) medium, 89 (10.2%) large, and 226 (25.9%) were calcified. The distribution of characterized cysts in different organs based on their size was found to be statistically significant (P < 0.05). In addition, out of the total 874 cysts collected, 26.9% were fertile, 47.3% sterile, and 25.9% calcified or purulent cysts. There was a significant difference in fertility of cyst from different organs (P < 0.05, chi(2) = 27.96), those of lung origin being highly fertile. Likewise, out of the 121 fertile cysts subjected for viability test, 68 (56.2%) were viable. Considering the current result, the total annual economic loss from organ condemnation and carcass weight loss due to bovine hydatidosis at Hawassa Municipal abattoir was estimated at 1,791,625.89 Ethiopian Birr (ETB; 1USD = 12.93ETB). Results in the study were discussed in light of the situation in different parts of Ethiopia and abroad, and finally, relevant recommendations were forwarded.
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Affiliation(s)
- Feyesa Regassa
- College of Agriculture and Veterinary Medicine, Jimma University, Jimma, Ethiopia.
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Torgerson P, Ziadinov I, Aknazarov D, Nurgaziev R, Deplazes P. Modelling the age variation of larval protoscoleces of Echinococcus granulosus in sheep. Int J Parasitol 2009; 39:1031-5. [DOI: 10.1016/j.ijpara.2009.01.004] [Citation(s) in RCA: 37] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/28/2022]
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Hydatidosis of slaughtered cattle in Wolaita Sodo Abattoir, southern Ethiopia. Trop Anim Health Prod 2008; 41:629-33. [PMID: 18787969 DOI: 10.1007/s11250-008-9234-2] [Citation(s) in RCA: 17] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/22/2008] [Accepted: 08/27/2008] [Indexed: 10/21/2022]
Abstract
The study was carried out from July 2007 to June 2008 in Wolaita Sodo Abattoir to assess the status of hydatidosis in cattle. Routine meat inspection, hydatid cyst count and characterization were conducted. Out of 400 cattle slaughtered in Wolaita Sodo Abattoir 64 (16%) animals were found harboring hydatid cysts. Thorough meat inspection in the abattoir revealed that 74 visceral organ were found harboring one or more hydatid cysts. The infection of the lung, liver, spleen and kidney were found to be 45.94% 45.94%, 6.75% and 1.35% respectively. From the total of 283 hydatid cysts counted 153(54.06%), 17(6.00%), 5(1.76%), 108(38.16%) were found to be small, medium, large and calcified cysts respectively and 170(60.28%), 5(1.76%) and 108(38.16%) were sterile, fertile and calcified cysts respectively. The rate of cyst calcification was higher in the liver than in the lung while fertility rate was higher among the cysts of the lung. Hydatid cyst viability rate of 40% was observed.
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Kebede N, Mitiku A, Tilahun G. Hydatidosis of slaughtered animals in Bahir Dar Abattoir, Northwestern Ethiopia. Trop Anim Health Prod 2008; 41:43-50. [PMID: 19052901 DOI: 10.1007/s11250-008-9152-3] [Citation(s) in RCA: 38] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/03/2008] [Accepted: 02/26/2008] [Indexed: 11/27/2022]
Abstract
The study was conducted from May 2005 to December 2006 in Bahir Dar Abattoir to assess the current status of hydatidosis in cattle and sheep. Hydatid cyst count and characterization were conducted based on routine meat inspection. Of the total 420 cattle and 340 sheep slaughtered in Bahir Dar Abattoir 143 (34.05%) and 36 (10.6%) animals were found harboring hydatid cysts respectively. Thorough meat inspection in the abattoir revealed that 202 and 54 visceral organs were found harboring one or more hydatid cysts in cattle and sheep respectively. Differences in prevalence rates between the two species of animals were highly significant (P < 0.001). The infection of the lung, liver, kidney, spleen and heart were found to be 57.9% , 36.6% , 3% , 1.5% , 1% in cattle and 50%, 48.1% and 1.9% in sheep respectively. From the total of 864 in cattle and 138 in sheep hydatid cysts counted 315 (36.4%), 268 (31.0%), 65 (7.5%), 216 (25.0%) in cattle and 92 (66.7%), 20 (14.5%), 1 (0.7%), 25 (18.1%) in sheep were found to be small, medium, large and calcified cysts respectively and 484 (56.0%), 164 (18.9%), 216 (25%) in cattle and 35 (25.4%), 78 (56.5%), 25 (18.1%) in sheep were sterile, fertile and calcified cysts respectively. Viability rates of 62.2% in cattle and 78.2% in sheep were observed. The rate of cyst calcification was higher in the liver than in the lung while fertility rate was higher among the cysts of the lung for both cattle and sheep.
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Affiliation(s)
- Nigatu Kebede
- Aklilu Lemma Institute of Pathobiology, Addis Ababa University, P. O. Box 56478, Addis Ababa, Ethiopia.
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21
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Christodoulopoulos G, Theodoropoulos G, Petrakos G. Epidemiological survey of cestode-larva disease in Greek sheep flocks. Vet Parasitol 2008; 153:368-73. [PMID: 18346853 DOI: 10.1016/j.vetpar.2008.02.002] [Citation(s) in RCA: 59] [Impact Index Per Article: 3.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/19/2007] [Revised: 01/24/2008] [Accepted: 02/05/2008] [Indexed: 02/07/2023]
Abstract
The epidemiological status of hydatidosis, cysticercosis, and gid in sheep and the production practices of sheep farmers that increase the risk of exposure of sheep to infecting agents of these diseases were investigated in Thessaly, central Greece during 2002-2006. A total of 700 hoggets (sheep up to the age of 1 year) and 1500 adult sheep were examined randomly at an abattoir survey and the prevalences of hydatidosis and cysticercosis were found 39.32% and 29.41%, respectively. Hoggets had significantly lower prevalences of hydatidosis and cysticercosis compared to adult sheep (p<0.001). The distribution of parasitic cysts showed an overdispersion pattern mostly in the hogget population. The proportion of fertile hydatid cysts was significantly higher in hoggets compared to adult sheep (p<0.001). A cohort of 74 sheep flocks was observed during the same period in a prospective survey to assess the incidence of gid. A total of 57 sheep belonging to 15 flocks (20.27% of investigated flocks) developed clinical signs of gid at an annual rate of 11.40+/-4.77 (95% C.I.). In the infected flocks there were 3.80+/-0.92 (95% C.I.) gid cases per flock. The mean age of onset of clinical signs for all sheep was 11.86+/-1.33 months (95% C.I.) (range 5-22 months) while for hoggets was 8.48+/-0.73 (95% C.I.) (range 5-12 months) and for adult sheep 17.23+/-1.42 (95% C.I.) (range 13-22 months). A significant difference was observed in the prevalence of unilateral blindness between hoggets (31.42%) and adult sheep (4.54%) (p<0.05). The most common production practices identified in a questionnaire survey of sheep farmers that may increase the risk of exposure of sheep to hydatidosis, cysticercosis, and gid were the improper disposal of dead animals, the access of farm dogs to offal of slaughtered sheep, the carelessness of farmers to treat farm dogs with anthelmintics, and the grazing of flocks in fields where stray dogs have free access.
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Affiliation(s)
- G Christodoulopoulos
- Clinic of Medicine, School of Veterinary Medicine, University of Thessaly, Karditsa 43100, Greece.
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Lahmar S, Lahmar S, Boufana B, Bradshaw H, Craig PS. Screening for Echinococcus granulosus in dogs: Comparison between arecoline purgation, coproELISA and coproPCR with necropsy in pre-patent infections. Vet Parasitol 2007; 144:287-92. [PMID: 17125928 DOI: 10.1016/j.vetpar.2006.10.016] [Citation(s) in RCA: 43] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/02/2006] [Revised: 10/20/2006] [Accepted: 10/23/2006] [Indexed: 11/30/2022]
Abstract
Echinococcus granulosus is an important zoonotic infection of dogs. The purpose of the present study assessed the performance of two laboratory diagnostic methods with arecoline purgation and necropsy in infected dogs. In total 65 dogs were successfully experimentally infected with protoscoleces of E. granulosus from ovine infection. At 14-34 days post-infection groups of dogs were purged with arecoline hydrobromide and then necropsied. Faecal samples were tested at weekly intervals by coproantigen ELISA and coproPCR. The necropsy infection rate with E. granulosus was 89.2 per cent. Only 43 per cent of dogs were successfully purged after one arecoline dose; this percentage increased to 76.9% for two doses of arecoline purgation. E. granulosus coproantigen was detected by coproELISA in 82.8% of faeces. The positive and negative predictive values for coproantigen ELISA were 96 and 44.4% respectively. E. granulosus DNA was detected in pre-patent faecal samples by coproPCR in 25.9% of dogs. These results indicate that coproELISA is more sensitive than arecoline purgation for the detection of pre-patent E. granulosus infection in dogs. CoproPCR detected E. granulosus DNA in dog faeces by 21 days post-infection before egg production.
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Affiliation(s)
- Selma Lahmar
- Parasitology Laboratory, National Veterinary School, 2020 Sidi Thabet, Tunisia
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Lahmar S, Chéhida FB, Pétavy AF, Hammou A, Lahmar J, Ghannay A, Gharbi HA, Sarciron ME. Ultrasonographic screening for cystic echinococcosis in sheep in Tunisia. Vet Parasitol 2006; 143:42-9. [PMID: 16973283 DOI: 10.1016/j.vetpar.2006.08.001] [Citation(s) in RCA: 41] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/27/2006] [Revised: 07/26/2006] [Accepted: 08/01/2006] [Indexed: 12/22/2022]
Abstract
Sheep from the areas of Fondouk-Jeddid, Bir Mchergua and El Fahs, located in the Northeast of Tunisia, were examined by ultrasonography between 2001 and 2004 in order to assess their infection with Echinococcus granulosus, the agent of hydatid disease, and to evaluate this method as an efficient aire for hydatid cysts. A total of 1039 sheep, aged between 1 and 14 years was examined. The highest prevalence was found in sheep aged more than 8 years. The least infected animals were aged between 1 and 2 years. All hydatid cysts detected by ultrasound were located in the liver. In all age-groups, the dead cysts were more numerous than viable cysts. Eighteen positive sheep were autopsied and a comparison between ultrasound and autopsy results was performed. The results showed a prevalence of about 40% for the three areas. Ultrasonography allowed the cysts, deep or superficial to localize in the central or left part in relation to the caudal vena cava of the animals. Consequently, all the cysts were not detected with this technique. This work shows that ultrasonography confirms the importance of ovine hydatid cyst in Tunisia and that its use as a mass screening approach for cystic echinococcosis in sheep could be helpful for the monitoring of this disease in a hydatid control program without great stress for the animals.
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Affiliation(s)
- S Lahmar
- Parasitology Laboratory, National Veterinary School, Sidi Thabet, Tunisia
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Abstract
Cystic echinococcosis (CE) caused by Echinococcus granulosus is a global public health problem. In many areas the disease is being diagnosed in increasing numbers, whilst in other areas it is re-emerging due to the collapse of public health programmes associated with socio-economic changes. Mathematical models of the transmission dynamics between animals can have an important role to play in developing control options. In particular the parasite is highly endemic in many lower income countries where resources to undertake an intensive control programme that has been successful in wealthy countries, such as New Zealand, are not available. Data from dogs and livestock have been collected and modelled from a number of different countries and regions. In Australia and New Zealand transmission modelling was first developed and these models have been refined using data from the Middle East and central Asia. The model indicates that relatively intense anthelmintic treatment of the dog population will result in a substantial decrease in the parasite population over time and has been supported by the results of control programmes. However, if the newly developed sheep vaccine is included in the control programme, then it should be possible to treat dogs less frequently to achieve the same result. This is due to the potentiating effects of attacking the parasite at two places in its life cycle. This should result in considerable cost savings over the use intensive anthelmintic treatment as the sole method of control.
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Affiliation(s)
- Paul R Torgerson
- WHO Collaborating Centre for Parasitic Zoonoses Institute of Parasitology, University of Zurich, Winterthurestrasse 266A, 8057 Zurich, Switzerland.
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Abstract
Echinococcosis is one of the major zoonotic parasitic diseases in the Middle East and Arabic North Africa from Morocco to Egypt. Both cystic and alveolar echinococcosis has been reported from these areas. However, cystic echinococcosis is more prevalent and has been reported from all countries in the Middle East and Arabic North Africa. Alveolar echinococcosis is less prevalent and has been reported only from Iran, Turkey, Iraq and Tunisia. Present situation of echinococcosis in dogs and other definitive hosts, animal intermediate hosts and humans in the Middle East and Arabic North Africa has been reviewed. Echinococcus granulosus is highly prevalent in Iran, Turkey, Iraq, Morocco, Tunisia, and Libya. In the Levant countries, the cystic echinococcosis is also highly endemic. In Oman, it is endemic with low prevalence and a very low level in Cyprus. Various surveys have indicated that hydatid cysts are commonly found in sheep, cattle, goats and camels throughout the Middle East and Arabic North Africa. Sheep are the most infected animals of these regions. Most of studies on human have been focused on surgical reports although several population studies have been performed using serological and imaging techniques. Human cystic echinococcosis (CE) is prevalent in the Middle East and Arabic North Africa. It is hyper endemic in Iran, Turkey, Iraq, Jordan, Morocco, Libya, Tunisia, and Algeria, and endemic in Egypt. Studies on the strain specificities of E. granulosus in the Middle East revealed sheep strain (G1) present in sheep, goats, cattle, camels and humans, and the camel strain (G6) in camels, sheep, cattle as well as humans. Dog/sheep strain seems to be more prevalent in the foregoing regions in documented reports from Iran and Jordan. However, a strain of E. granulosus, which resembles the horse strain (G4) strain, has been reported from Jordan. Strain specifications of E. granulosus in Arabic North Africa showed that sheep/dog strain (G1) have been reported from Tunisia and Libya both from humans and animals. However, in Egypt the human cases reported are of camel/dog strain.
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Affiliation(s)
- Seyed Mahmoud Sadjjadi
- Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, P.O. Box 71345-1735, Iran.
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M'rad S, Filisetti D, Oudni M, Mekki M, Belguith M, Nouri A, Sayadi T, Lahmar S, Candolfi E, Azaiez R, Mezhoud H, Babba H. Molecular evidence of ovine (G1) and camel (G6) strains of Echinococcus granulosus in Tunisia and putative role of cattle in human contamination. Vet Parasitol 2005; 129:267-72. [PMID: 15845282 DOI: 10.1016/j.vetpar.2005.02.006] [Citation(s) in RCA: 51] [Impact Index Per Article: 2.6] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/10/2004] [Revised: 02/01/2005] [Accepted: 02/17/2005] [Indexed: 02/07/2023]
Abstract
Three hundred and seventy-two cysts coming from 50 humans, 166 cattle, 153 sheep and 3 camels were collected in order to establish some epidemiological molecular information in Tunisia for the first time. The analysis by PCR-RFLP of ITS1 sequence showed that all the human, ovine and bovine cysts were due to the common sheep strain of Echinococcus granulosus. The sequencing of the CO1 gene of 37 isolates confirm the G1 genotype of this strain. For seven of these isolates, we found the mutation C56T which is present in the three principal intermediate hosts: human (three cysts), cattle (three cysts) and sheep (one cyst). With regard to the G1 genotype, we identified three other point mutations. The camel strain G6 is uniquely found in the three camels isolates and not in the other intermediate hosts analysed. The fertility of the bovine cyst represents 48% that means that this host is involved in a bovine-dog cycle and consequently represents a reservoir of sheep strain in Tunisia. Our results confirm the importance of the prophylaxis measures in order to disrupt the cycle of transmission sheep-dog in Tunisia. Nevertheless, the supervision of bovine infection should be reinforced because this intermediate host may constitute an important link with the human contamination.
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Affiliation(s)
- S M'rad
- Laboratoire de Parasitologie-Mycologie, Code 99UR/08-05, Département de Biologie Clinique B, Faculté de Pharmacie, 5000 Monastir, Tunisie.
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Majorowski MM, Carabin H, Kilani M, Bensalah A. Echinococcosis in Tunisia: a cost analysis. Trans R Soc Trop Med Hyg 2005; 99:268-78. [PMID: 15708386 DOI: 10.1016/j.trstmh.2004.06.011] [Citation(s) in RCA: 48] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/30/2003] [Revised: 06/22/2004] [Accepted: 06/22/2004] [Indexed: 11/19/2022] Open
Abstract
Echinococcus granulosus infection is a preventable zoonosis of human and veterinary public health importance in Tunisia. We aimed to comprehensively quantify human and animal echinococcosis losses in Tunisia. Itemized cost menus were developed for the health of both domestic animals and humans, and for productivity monetary losses. The incidence and prevalence of the disease in all species were obtained from national and personal reports. The domestic animal and human costs of echinococcosis in Tunisia were estimated using age-stratified rates and losses, productivity losses (including those not formally employed) and Monte Carlo sampling to represent the uncertainty inherent in some epidemiological and economic values. Echinococcosis in Tunisia causes significant direct and indirect losses in both humans and animals of approximately US dollars 10-19 million annually. These estimates are based on numerous methodological improvements over previous studies and are of considerable consequence relative to Tunisia's US dollars 21.2 billion gross domestic product. A cost-benefit analysis of control programmes using the methodological advances presented here and regional comparison to other endemic diseases is warranted. These may provide information to assist policy decision-makers in prioritizing the allocation of scarce resources.
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Affiliation(s)
- Meghan M Majorowski
- Wellcome Trust Centre for the Epidemiology of Infectious Diseases, University of Oxford, Oxford, UK.
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Elmahdi IE, Ali QM, Magzoub MMA, Ibrahim AM, Saad MB, Romig T. Cystic echinococcosis of livestock and humans in central Sudan. ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY 2004; 98:473-9. [PMID: 15257797 DOI: 10.1179/000349804225003578] [Citation(s) in RCA: 41] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 10/31/2022]
Abstract
New information was collected on cystic echinococcosis in livestock (camels, cattle and sheep) and humans in the central region of Sudan. The livestock data were collected in abattoir-based surveys in the towns of Omdurman, Tamboul and Wad Madani, between 1998 and 2001, and covered a total of 8205 animals. The highest prevalence of infection was found in the camels (44.6% of 242 infected), followed by the sheep (6.9% of 5595) and cattle (3.0% of 2368). Records were made of the sizes of the 1320 hydatid cysts detected in the livestock (907 in sheep, 71 in cattle, and 342 in camels), whether or not each cyst was fertile, and where it occurred in the body of the host. Cysts collected from cattle and camels where much more likely to be fertile (22% and 24%, respectively) than those from sheep (1%). Camels and cattle therefore appear to be the principal intermediate hosts for Echinococcus granulosus in central Sudan, whereas sheep apparently play a marginal role in transmission. In 2002, as a preliminary assessment of the public-health impact of the disease, 300 residents of a rural village 60 km west of Wad Madani were surveyed using a portable ultrasound scanner. Only one (0.33%) of the villagers investigated was found infected. The implications of these finding are discussed in terms of the various strains of E. granulosus and the role of each in human disease.
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Affiliation(s)
- I E Elmahdi
- Institute of Nuclear Medicine, Molecular Biology and Oncology, University of Gezira, P. O. Box 20, Gezira, Sudan
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Lahmar S, Debbek H, Zhang LH, McManus DP, Souissi A, Chelly S, Torgerson PR. Transmission dynamics of the Echinococcus granulosus sheep-dog strain (G1 genotype) in camels in Tunisia. Vet Parasitol 2004; 121:151-6. [PMID: 15110412 DOI: 10.1016/j.vetpar.2004.02.016] [Citation(s) in RCA: 52] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/10/2003] [Revised: 01/29/2004] [Accepted: 02/09/2004] [Indexed: 02/07/2023]
Abstract
Cystic echinococcosis, caused by Echinococcus granulosus, is highly endemic in North Africa and the Middle East. This paper examines the abundance and prevalence of infection of E. granulosus in camels in Tunisia. No cysts were found in 103 camels from Kébili, whilst 19 of 188 camels from Benguerden (10.1%) were infected. Of the cysts found 95% were considered fertile with the presence of protoscolices and 80% of protoscolices were considered viable by their ability to exclude aqueous eosin. Molecular techniques were used on cyst material from camels and this demonstrated that the study animals were infected with the G1 sheep strain of E. granulosus. Observed data were fitted to a mathematical model by maximum likelihood techniques to define the parameters and their confidence limits and the negative binomial distribution was used to define the error variance in the observed data. The infection pressure to camels was somewhat lower in comparison to sheep reported in an earlier study. However, because camels are much longer-lived animals, the results of the model fit suggested that older camels have a relatively high prevalence rate, reaching a most likely value of 32% at age 15 years. This could represent an important source of transmission to dogs and hence indirectly to man of this zonotic strain. In common with similar studies on other species, there was no evidence of parasite-induced immunity in camels.
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MESH Headings
- Animals
- Camelus/parasitology
- DNA, Mitochondrial/chemistry
- DNA, Mitochondrial/genetics
- DNA, Protozoan/chemistry
- DNA, Protozoan/genetics
- Echinococcosis, Hepatic/epidemiology
- Echinococcosis, Hepatic/parasitology
- Echinococcosis, Hepatic/transmission
- Echinococcosis, Hepatic/veterinary
- Echinococcosis, Pulmonary/epidemiology
- Echinococcosis, Pulmonary/parasitology
- Echinococcosis, Pulmonary/transmission
- Echinococcosis, Pulmonary/veterinary
- Echinococcus/classification
- Echinococcus/genetics
- Echinococcus/isolation & purification
- Electron Transport Complex IV/chemistry
- Electron Transport Complex IV/genetics
- Polymerase Chain Reaction/veterinary
- Prevalence
- Tunisia/epidemiology
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Affiliation(s)
- S Lahmar
- Service de Parasitologie, Ecole Nationale de Médecine Vétérinaire, 2020 Sidi Thabet, Tunisia
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Oudni M, M'rad S, Mekki M, Belguith M, Cabaret J, Pratlong F, Sayadi T, Nouri A, Mezhoud H, Babba H, Azaiez R. Genetic relationships between sheep, cattle and human Echinococcus infection in Tunisia. Vet Parasitol 2004; 121:95-103. [PMID: 15110407 DOI: 10.1016/j.vetpar.2004.01.019] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/17/2003] [Revised: 01/20/2004] [Accepted: 01/27/2004] [Indexed: 11/20/2022]
Abstract
Allozyme variation at seven polymorphic loci (GPI, EST, MDH, MPI, DIA, PEP, PGM) was studied to examine genetic variation within and between sheep, cattle and human populations of Echinococcus granulosus in Tunisia. A high degree of genetic similarity was shown between the cysts of the three host origins. Nevertheless, whereas, the ovine and human samples were highly similar, the cattle samples were slightly different genetically. We conclude that humans are mostly infected by parasites originating from sheep liver. The intense deficiency in heterozygotes was partly artefactual (Wahlund effect) and partly due to self-fertilisation.
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Affiliation(s)
- M Oudni
- Laboratoire de Parasitologie-Mycologie, Code 99UR/08-05, Département de Biologie clinique B, Faculté de Pharmacie, 5000 Monastir, Tunisia.
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Eckert J, Deplazes P. Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin Microbiol Rev 2004; 17:107-35. [PMID: 14726458 PMCID: PMC321468 DOI: 10.1128/cmr.17.1.107-135.2004] [Citation(s) in RCA: 1170] [Impact Index Per Article: 55.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/20/2022] Open
Abstract
Echinococcosis in humans is a zoonotic infection caused by larval stages (metacestodes) of cestode species of the genus Echinococcus. Cystic echinococcosis (CE) is caused by Echinococcus granulosus, alveolar echinococcosis (AE) is caused by E. multilocularis, and polycystic forms are caused by either E. vogeli or E. oligarthrus. In untreated cases, AE has a high mortality rate. Although control is essentially feasible, CE remains a considerable health problem in many regions of the northern and southern hemispheres. AE is restricted to the northern hemisphere regions of North America and Eurasia. Recent studies have shown that E. multilocularis, the causative agent of AE, is more widely distributed than previously thought. There are also some hints of an increasing significance of polycystic forms of the disease, which are restricted to Central and South America. Various aspects of human echinococcosis are discussed in this review, including data on the infectivity of genetic variants of E. granulosus to humans, the increasing invasion of cities in Europe and Japan by red foxes, the main definitive hosts of E. multilocularis, and the first demonstration of urban cycles of the parasite. Examples of emergence or reemergence of CE are presented, and the question of potential spreading of E. multilocularis is critically assessed. Furthermore, information is presented on new and improved tools for diagnosing the infection in final hosts (dogs, foxes, and cats) by coproantigen or DNA detection and the application of molecular techniques to epidemiological studies. In the clinical field, the available methods for diagnosing human CE and AE are described and the treatment options are summarized. The development of new chemotherapeutic options for all forms of human echinococcosis remains an urgent requirement. A new option for the control of E. granulosus in the intermediate host population (mainly sheep and cattle) is vaccination. Attempts are made to reduce the prevalence of E. multilocualaris in fox populations by regular baiting with an anthelmintic (praziquantel). Recent data have shown that this control option may be used in restricted areas, for example in cities, with the aim of reducing the infection risk for humans.
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Affiliation(s)
- Johannes Eckert
- Institute of Parasitology, University of Zurich, CH-8057 Zurich, Switzerland.
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Umur S. Prevalence and economic importance of cystic echinococcosis in slaughtered ruminants in Burdur, Turkey. JOURNAL OF VETERINARY MEDICINE. B, INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH 2003; 50:247-52. [PMID: 12864901 DOI: 10.1046/j.1439-0450.2003.00667.x] [Citation(s) in RCA: 40] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/13/2022]
Abstract
This study was conducted between April 2000 and March 2001, in 12-month period. During the study, local slaughterhouses were visited periodically for 1 year to examine the internal organs (livers, lungs, spleens and hearts) for the presence of cysts and total 1355 cattle, 218 sheep and 104 goats were examined for the cystic echinococcosis (CE). It was found that 13.5% of cattle, 26.6% of sheep and 22.1% of goats were infected with this disease. While cysts in cattle (P < 0.001) and goats (P > 0.05) were found mostly in lungs (88.5 and 82.6%, respectively), but they were mostly found in livers (P > 0.05) in sheep. In addition to this, three spleens and one heart in cattle were infected with CE. In this study, the prevalence of CE and the number of cysts in ruminants were found different when the cattle, sheep and goats examined were stratified based on age. The prevalence and the number of cysts increased with age approaching an asymptotic prevalence of one in the oldest animals (P < 0.05). The number of cysts in cattle, sheep and goats were increasing at a rate of 0.31, 0.63 and 0.42/year, respectively. The economic decrease in the value of the carcasses because of the discarded liver and lung as a result of CE was estimated as 1.1% (7.5 US dollars per cattle) for cattle, 4.37% (3.2 US dollars per sheep) for sheep and 4.26% (2.9 US dollars per goat) for goats. The minimum total loss for all infected animals was determined to be 583 US dollars in infected animals, based on the market prices in the year 2002.
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Affiliation(s)
- S Umur
- Department of Parasitology, Faculty of Veterinary Medicine, Akdeniz University, 15100, Burdur, Turkey.
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Torgerson PR, Burtisurnov KK, Shaikenov BS, Rysmukhambetova AT, Abdybekova AM, Ussenbayev AE. Modelling the transmission dynamics of Echinococcus granulosus in sheep and cattle in Kazakhstan. Vet Parasitol 2003; 114:143-53. [PMID: 12781476 DOI: 10.1016/s0304-4017(03)00136-5] [Citation(s) in RCA: 53] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/22/2022]
Abstract
Cystic echinococcosis, caused by Echinococcus granulosus, is an emerging disease in many parts of the world and, in particular, in eastern Europe and the former Soviet Union. This paper examines the abundance and prevalence of infection of E. granulosus in cattle and sheep in Kazakhstan. Observed data are fitted to a mathematical model in order to determine if the parasite population is partly regulated by intermediate host immunity and to define parameters in the model. Such data would be useful to develop simulation models for the control of this disease. Maximum likelihood techniques were used to define the parameters and their confidence limits in the model and the negative binomial distribution was used to define the error variance in the observed data. The results indicated that there are significant variations in the infection pressure to sheep depending on their location. In particular sheep from Almaty Oblast and from central and northern Kazakhstan appeared to have a greater exposure than sheep from Jambyl or South Kazakhstan Oblasts. The infection pressure to cattle was somewhat lower in comparison. In common with other similar studies, there was no evidence of parasite-induced immunity in sheep or cattle in Kazakhstan due to natural infection. The highest abundance and prevalence were seen in the oldest age classes of animals.
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Affiliation(s)
- P R Torgerson
- Institute of Parasitology, University of Zürich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland.
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Abstract
In many parts of the world Echinococcus granulosus is a widespread infection in sheep and dogs with a consequential spill over into the human population. In the past, mathematical models have been derived to define the transmission dynamics of this parasite, principally in the sheep-dog life cycle. These models have characterized the cycles of infection as lacking in density dependent constraints in both the definitive or intermediate hosts. This suggested that there was little, if any, induced host immunity by the parasite in either host in natural infections. However, recent evidence from both Tunisia and Kazakhstan, where young dogs are the most heavily parasitised, suggests the possibility of significant definitive host immunity. This may have an effect on the control effort needed to destabilize the parasite. A preliminary computer simulation model (based on an Excel spreadsheet) to attempt to predict the results of a control programme has been written. This demonstrates that there could be significantly different results if there is indeed protective immunity in the dog than in the absence of immunity. In the former the parasite needs a greater control effort to push the parasite towards extinction than in the latter. The computer simulation is based on a mathematical model of the parasite's life cycle and is flexible so that different values of parameters can be used in different situations where the transmission of the parasite may be at different levels. Because of the flexibility of the computer simulation it is anticipated that this programme can be applied in most situations, although initial parameters for a particular location or strain of the parasite will have to be first predetermined with base line field surveys and possibly experimental infections. The programme also has an additional flexibility to enable simulations if some parameters cannot be accurately estimated through Monte-Carlo techniques. In the latter situation, worst and best case scenarios can be estimated and likely frequency distributions of the unknown parameters can be included in the model.
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Affiliation(s)
- P R Torgerson
- Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, University College Dublin, Ballsbridge, 4, Dublin, Ireland.
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Tashani OA, Zhang LH, Boufana B, Jegi A, McManus DP. Epidemiology and strain characteristics of Echinococcus granulosus in the Benghazi area of eastern Libya. ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY 2002; 96:369-81. [PMID: 12171618 DOI: 10.1179/000349802125000952] [Citation(s) in RCA: 47] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/28/2022]
Abstract
The incidence of surgically confirmed cystic echinococcosis in eastern Libya was estimated to be at least 4.2 cases/100,000, with significantly more female cases than male. The prevalences of infection with Echinococcus granulosus among 1087 sheep, 881 goats, 428 camels and 614 cattle from the same region, determined postmortem in abattoirs, were 20%, 3.4%, 13.6% and 11%, respectively. Infection in the livestock was age-dependent and, generally, the female animals were more often infected than the male. The measurements of rostellar hooks on protoscoleces collected from sheep and cattle were similar but significantly different from the corresponding measurements of parasites of human or camel origin. However, when a portion of the cytochrome c-oxidase subunit I (cox1) gene from each of 30 protoscolex samples from Libya (12 from cattle, three from humans, five from camels and 10 from sheep) was sequenced, the sequences were all found to be identical to that published for the common sheep strain of E. granulosus.
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Affiliation(s)
- O A Tashani
- Department of Zoology, Garyounis University, P.O. Box 17192, Benghazi, Libya.
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