Reference Citation Analysis: Find an Article, Find a Category, Find a Journal, Find a Scholar

For:	Meredith JE, Schwartz MA. Integrins, adhesion and apoptosis. Trends Cell Biol 2012;7:146-50. [PMID: 17708932 DOI: 10.1016/s0962-8924(97)01002-7] [Citation(s) in RCA: 197] [Impact Index Per Article: 15.2] [Reference Citation Analysis] [What about the content of this article? (0)] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/22/2023]

Number

Cited by Other Article(s)

Schallmoser A, Emrich N, Einenkel R, Sänger N. Explorative 3-D culture of early secondary follicles in a time lapse system for up to 36 days gives valuable, but limited insights in follicular development. Placenta 2025;164:50-63. [PMID: 40127611 DOI: 10.1016/j.placenta.2025.03.007] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 07/24/2024] [Revised: 02/10/2025] [Accepted: 03/10/2025] [Indexed: 03/26/2025]

Abstract

BACKGROUND

Cryopreservation of ovarian cortical tissue is an important option for female fertility preservation. This is particularly valuable for cancer patients who need to be treated urgently with chemotherapy, leaving no time for hormonal stimulation. The transfer of malignant cells in certain cancers remains as a potential risk after freezing, thawing and transplantation of ovarian tissue while isolation and in vitro growth (IVG) of follicles could be a safe alternate approach of female fertility protection.

METHODS

Ovarian cortex tissue was frozen, thawed and cultured for 8 days prior to isolating and embedding of early secondary follicles in a 3D matrix, suitable for time lapse monitoring for up to 36 days. Continuous growth of a theca-like cell layer and extrafollicular protrusions were visually evaluated with a permanent monitoring system facilitating real-time follicular development without deviations in the culture conditions. Occurrence of theca cell growth was visually characterized by extrafollicular formation of cells, beyond the outer follicle boundaries. To validate the results observed by time-lapse monitoring, live cell imaging was conducted and determined with immunofluorescence staining.

RESULTS

Individual follicles significantly increased in size over time. Time-lapse video monitoring revealed extending and retracting of filopodia-like structures in the outer follicular region adjacent to the 3D environment. Theca-like cells and actin components of filopodia-like structures were identified based on immunofluorescence staining.

CONCLUSIONS

Time lapse monitoring of 3-D cultured follicles is a promising explorative approach to obtain valuable visual insights regarding the many facets of follicular growth and to optimize follicular culture conditions towards a clinical application. As the study is limited by a lack of mechanistic insights into theca cell differentiation and filopodia function, additional studies are necessary to validate the preliminary results of this approach.

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Hudacova E, Abaffy P, Kaplan MM, Krausova M, Kubista M, Machon O. Single-cell transcriptomic resolution of osteogenesis during craniofacial morphogenesis. Bone 2025;190:117297. [PMID: 39461490 DOI: 10.1016/j.bone.2024.117297] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 07/12/2024] [Revised: 10/07/2024] [Accepted: 10/15/2024] [Indexed: 10/29/2024]

Ndinyanka Fabrice T, Buczak K, Schmidt A, Pieters J. T cell population size control by coronin 1 uncovered: from a spot identified by two-dimensional gel electrophoresis to quantitative proteomics. Expert Rev Proteomics 2025;22:35-44. [PMID: 39849824 DOI: 10.1080/14789450.2025.2450812] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/20/2024] [Revised: 10/28/2024] [Accepted: 12/19/2024] [Indexed: 01/25/2025]

Wang Z, Xie D, Li J, Zhai Z, Lu Z, Tian X, Niu Y, Zhao Q, Zheng P, Dong L, Wang C. Molecular force-induced liberation of transforming growth factor-beta remodels the spleen for ectopic liver regeneration. J Hepatol 2024;80:753-763. [PMID: 38244845 DOI: 10.1016/j.jhep.2024.01.005] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 08/27/2023] [Revised: 12/08/2023] [Accepted: 01/04/2024] [Indexed: 01/22/2024]

Abstract

BACKGROUND & AIMS

Ectopic liver regeneration in the spleen is a promising alternative to organ transplantation for treating liver failure. To accommodate transplanted liver cells, the splenic tissue must undergo structural changes to increase extracellular matrix content, demanding a safe and efficient approach for tissue remodelling.

METHODS

We synthesised sulphated hyaluronic acid (sHA) with an affinity for the latent complex of transforming growth factor-β (TGF-β) and cross-linked it into a gel network (sHA-X) via click chemistry. We injected this glycan into the spleens of mice to induce splenic tissue remodelling via supraphysiological activation of endogenous TGF-β.

RESULTS

sHA-X efficiently bound to the abundant latent TGF-β in the spleen. It provided the molecular force to liberate the active TGF-β dimers from their latent complex, mimicking the 'bind-and-pull' mechanism required for physiological activation of TGF-β and reshaping the splenic tissue to support liver cell growth. Hepatocytes transplanted into the remodelled spleen developed into liver tissue with sufficient volume to rescue animals with a metabolic liver disorder (Fah-/- transgenic model) or following 90% hepatectomy, with no adverse effects observed and no additional drugs required.

CONCLUSION

Our findings highlight the efficacy and translational potential of using sHA-X to remodel a specific organ by mechanically activating one single cytokine, representing a novel strategy for the design of biomaterials-based therapies for organ regeneration.

IMPACT AND IMPLICATIONS

Cell transplantation may provide a lifeline to millions of patients with end-stage liver diseases, but their severely damaged livers being unable to accommodate the transplanted cells is a crucial hurdle. Herein, we report an approach to restore liver functions in another organ - the spleen - by activating one single growth factor in situ. This approach, based on a chemically designed polysaccharide that can mechanically liberate the active transforming growth factor-β to an unusually high level, promotes the function of abundant allogenic liver cells in the spleen, rescuing animals from lethal models of liver diseases and showing a high potential for clinical translation.

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Affiliation(s)

Zhenzhen Wang State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China; State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau SAR, China
Daping Xie State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau SAR, China
Jiayi Li State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China
Ziyu Zhai State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China
Zhuojian Lu State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, 210023, China
Xuejiao Tian State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China
Yiming Niu State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau SAR, China
Qi Zhao Department of Biomedical Sciences, Faculty of Health Science, University of Macau, Taipa, Macau SAR, China
Peng Zheng State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, 210023, China; Chemistry and Biomedicine Innovative Center, Nanjing University, Nanjing, Jiangsu 210023, China.
Lei Dong State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China; National Resource Center for Mutant Mice, Nanjing, Jiangsu, 210023, China; Chemistry and Biomedicine Innovative Center, Nanjing University, Nanjing, Jiangsu 210023, China.
Chunming Wang State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau SAR, China; Department of Pharmaceutical Sciences, Faculty of Health Science, University of Macau, Taipa, Macau SAR, China.

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Brandhorst H, Krishtul S, Brandhorst D, Baruch L, Machluf M, Johnson PRV. Solubilized Pancreatic Extracellular Matrix from Juvenile Pigs Protects Isolated Human Islets from Hypoxia-Induced Damage: A Viable Option for Clinical Islet Transplantation. J Tissue Eng Regen Med 2023;2023:7452682. [PMID: 40226393 PMCID: PMC11918917 DOI: 10.1155/2023/7452682] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/26/2023] [Revised: 05/26/2023] [Accepted: 06/07/2023] [Indexed: 04/15/2025]

Halawi A, El Kurdi AB, Vernon KA, Solhjou Z, Choi JY, Saad AJ, Younis NK, Elfekih R, Mohammed MT, Deban CA, Weins A, Abdi R, Riella LV, De Serres SA, Cravedi P, Greka A, Khoueiry P, Azzi JR. Uncovering a novel role of focal adhesion and interferon-gamma in cellular rejection of kidney allografts at single cell resolution. Front Immunol 2023;14:1139358. [PMID: 37063857 PMCID: PMC10102512 DOI: 10.3389/fimmu.2023.1139358] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/06/2023] [Accepted: 02/23/2023] [Indexed: 04/03/2023] Open

Affiliation(s)

Ahmad Halawi Transplantation Research Center, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, United States
Abdullah B. El Kurdi Department of Biochemistry and Molecular Genetics, Faculty of Medicine, American University of Beirut, Beirut, Lebanon
Katherine A. Vernon Q32 Bio, Waltham, MA, United States
Zhabiz Solhjou Transplantation Research Center, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, United States Scripps Clinic Medical Group, San Diego, CA, United States
John Y. Choi Transplantation Research Center, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, United States
Anis J. Saad Transplantation Research Center, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, United States
Nour K. Younis Transplantation Research Center, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, United States
Rania Elfekih Transplantation Research Center, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, United States
Mostafa Tawfeek Mohammed Transplantation Research Center, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, United States Clinical Pathology Department, Faculty of Medicine, Minia University, Minia, Egypt
Christa A. Deban Transplantation Research Center, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, United States
Astrid Weins Department of Pathology, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, United States
Reza Abdi Transplantation Research Center, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, United States
Leonardo V. Riella Department of Medicine, Division of Nephrology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States Center for Transplantation Sciences, Department of Surgery, Massachusetts General Hospital, Boston, MA, United States
Sasha A. De Serres Transplantation Unit, Renal Division, Department of Medicine, University Health Center of Quebec, Faculty of Medicine, Laval University, Québec, QC, Canada
Paolo Cravedi Translational Transplant Research Center, Icahn School of Medicine at Mount Sinai, New York, NY, United States
Anna Greka The Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, United States Department of Medicine, Brigham and Women’s Hospital, Boston, MA, United States
Pierre Khoueiry Department of Biochemistry and Molecular Genetics, Faculty of Medicine, American University of Beirut, Beirut, Lebanon
Jamil R. Azzi Transplantation Research Center, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, United States *Correspondence: Jamil R. Azzi,

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Ndinyanka Fabrice T, Bianda C, Zhang H, Jayachandran R, Ruer-Laventie J, Mori M, Moshous D, Fucile G, Schmidt A, Pieters J. An evolutionarily conserved coronin-dependent pathway defines cell population size. Sci Signal 2022;15:eabo5363. [DOI: 10.1126/scisignal.abo5363] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/09/2022]

Hunter EJ, Hamaia SW, Gullberg D, Malcor JD, Farndale RW. Selectivity of the collagen-binding integrin inhibitors, TC-I-15 and obtustatin. Toxicol Appl Pharmacol 2021;428:115669. [PMID: 34363821 PMCID: PMC8444087 DOI: 10.1016/j.taap.2021.115669] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/13/2021] [Revised: 07/29/2021] [Accepted: 08/02/2021] [Indexed: 11/22/2022]

Pouw AE, Greiner MA, Coussa RG, Jiao C, Han IC, Skeie JM, Fingert JH, Mullins RF, Sohn EH. Cell-Matrix Interactions in the Eye: From Cornea to Choroid. Cells 2021;10:687. [PMID: 33804633 PMCID: PMC8003714 DOI: 10.3390/cells10030687] [Citation(s) in RCA: 50] [Impact Index Per Article: 12.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/14/2021] [Revised: 03/03/2021] [Accepted: 03/16/2021] [Indexed: 02/07/2023] Open

Affiliation(s)

Andrew E. Pouw Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa Hospitals & Clinics, Iowa City, IA 52242, USA; (A.E.P.); (M.A.G.); (R.G.C.); (C.J.); (I.C.H.); (J.M.S.); (J.H.F.); (R.F.M.) Institute for Vision Research, University of Iowa, Iowa City, IA 52242, USA
Mark A. Greiner Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa Hospitals & Clinics, Iowa City, IA 52242, USA; (A.E.P.); (M.A.G.); (R.G.C.); (C.J.); (I.C.H.); (J.M.S.); (J.H.F.); (R.F.M.) Institute for Vision Research, University of Iowa, Iowa City, IA 52242, USA
Razek G. Coussa Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa Hospitals & Clinics, Iowa City, IA 52242, USA; (A.E.P.); (M.A.G.); (R.G.C.); (C.J.); (I.C.H.); (J.M.S.); (J.H.F.); (R.F.M.) Institute for Vision Research, University of Iowa, Iowa City, IA 52242, USA
Chunhua Jiao Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa Hospitals & Clinics, Iowa City, IA 52242, USA; (A.E.P.); (M.A.G.); (R.G.C.); (C.J.); (I.C.H.); (J.M.S.); (J.H.F.); (R.F.M.) Institute for Vision Research, University of Iowa, Iowa City, IA 52242, USA
Ian C. Han Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa Hospitals & Clinics, Iowa City, IA 52242, USA; (A.E.P.); (M.A.G.); (R.G.C.); (C.J.); (I.C.H.); (J.M.S.); (J.H.F.); (R.F.M.) Institute for Vision Research, University of Iowa, Iowa City, IA 52242, USA
Jessica M. Skeie Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa Hospitals & Clinics, Iowa City, IA 52242, USA; (A.E.P.); (M.A.G.); (R.G.C.); (C.J.); (I.C.H.); (J.M.S.); (J.H.F.); (R.F.M.)
John H. Fingert Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa Hospitals & Clinics, Iowa City, IA 52242, USA; (A.E.P.); (M.A.G.); (R.G.C.); (C.J.); (I.C.H.); (J.M.S.); (J.H.F.); (R.F.M.) Institute for Vision Research, University of Iowa, Iowa City, IA 52242, USA
Robert F. Mullins Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa Hospitals & Clinics, Iowa City, IA 52242, USA; (A.E.P.); (M.A.G.); (R.G.C.); (C.J.); (I.C.H.); (J.M.S.); (J.H.F.); (R.F.M.) Institute for Vision Research, University of Iowa, Iowa City, IA 52242, USA
Elliott H. Sohn Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa Hospitals & Clinics, Iowa City, IA 52242, USA; (A.E.P.); (M.A.G.); (R.G.C.); (C.J.); (I.C.H.); (J.M.S.); (J.H.F.); (R.F.M.) Institute for Vision Research, University of Iowa, Iowa City, IA 52242, USA

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Real-time and wide-field mapping of cell-substrate adhesion gap and its evolution via surface plasmon resonance holographic microscopy. Biosens Bioelectron 2021;174:112826. [PMID: 33262060 DOI: 10.1016/j.bios.2020.112826] [Citation(s) in RCA: 15] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/27/2020] [Revised: 10/17/2020] [Accepted: 11/14/2020] [Indexed: 12/11/2022]

Beauséjour M, Boutin A, Vachon PH. Anoikis and the Human Gut Epithelium in Health and Disease. ANOIKIS 2021:95-126. [DOI: 10.1007/978-3-030-73856-3_5] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/02/2025]

Wippold JA, Wang H, Tingling J, Leibowitz J, de Figueiredo P, Han A. PRESCIENT: platform for the rapid evaluation of antibody success using integrated microfluidics enabled technology. LAB ON A CHIP 2020;20:1628-1638. [PMID: 32196032 PMCID: PMC7269184 DOI: 10.1039/c9lc01165j] [Citation(s) in RCA: 18] [Impact Index Per Article: 3.6] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/11/2023]

Abstract

Identifying antibodies (Abs) that neutralize infectious agents is the first step for developing therapeutics, vaccines, and diagnostic tools for these infectious agents. However, current approaches for identifying neutralizing Abs (nAbs) typically rely on dilution-based assays that are costly, inefficient, and only survey a small subset of the entire repertoire. There are also intrinsic biases in many steps of conventional nAb identification processes. More importantly, conventional assays rely on simple Ab-antigen binding assays, which may not result in identifying the most potent nAbs, as the strongest binder may not be the most potent nAb. Droplet microfluidic systems have the capability to overcome such limitations by conducting complex multi-step assays with high reliability, resolution, and throughput in a pico-liter volume water-in-oil emulsion droplet format. Here, we describe the development of PRESCIENT (Platform for the Rapid Evaluation of antibody SucCess using Integrated microfluidics ENabled Technology), a droplet microfluidic system that can enable high-throughput single-cell resolution identification of nAb repertoires elicited in response to viral infection. We demonstrate PRESCIENT's ability to identify Abs that neutralize a model viral agent, Murine coronavirus (murine hepatitis virus), which causes high mortality rates in experimentally infected mice. In-droplet infection of host cells by the virus was first demonstrated, followed by demonstration of in-droplet neutralization by nAbs produced from a single Ab-producing hybridoma cell. Finally, fluorescence intensity analyses of two populations of hybridoma cell lines (nAb-producing and non-nAb-producing hybridoma cell lines) successfully discriminated between the two populations. The presented strategy and platform have the potential to identify and investigate neutralizing activities against a broad range of potential infectious agents for which nAbs have yet to be discovered, significantly advancing the nAb identification process as well as reinvigorating the field of Ab discovery, characterization, and development.

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Nygren P, Balashova N, Brown AC, Kieba I, Dhingra A, Boesze-Battaglia K, Lally ET. Aggregatibacter actinomycetemcomitans leukotoxin causes activation of lymphocyte function-associated antigen 1. Cell Microbiol 2018;21:e12967. [PMID: 30329215 DOI: 10.1111/cmi.12967] [Citation(s) in RCA: 12] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/01/2018] [Revised: 09/20/2018] [Accepted: 10/05/2018] [Indexed: 11/29/2022]

Szekacs I, Farkas E, Gemes BL, Takacs E, Szekacs A, Horvath R. Integrin targeting of glyphosate and its cell adhesion modulation effects on osteoblastic MC3T3-E1 cells revealed by label-free optical biosensing. Sci Rep 2018;8:17401. [PMID: 30479368 PMCID: PMC6258691 DOI: 10.1038/s41598-018-36081-0] [Citation(s) in RCA: 21] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/16/2018] [Accepted: 10/26/2018] [Indexed: 12/14/2022] Open

Wang Y, Zhang T, Guo L, Ren T, Yang Y. Stromal extracellular matrix is a microenvironmental cue promoting resistance to EGFR tyrosine kinase inhibitors in lung cancer cells. Int J Biochem Cell Biol 2018;106:96-106. [PMID: 30471423 DOI: 10.1016/j.biocel.2018.11.001] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/06/2018] [Revised: 11/01/2018] [Accepted: 11/06/2018] [Indexed: 02/07/2023]

Kim TH, Zhou H. Overexpression of Chicken IRF7 Increased Viral Replication and Programmed Cell Death to the Avian Influenza Virus Infection Through TGF-Beta/FoxO Signaling Axis in DF-1. Front Genet 2018;9:415. [PMID: 30356848 PMCID: PMC6190866 DOI: 10.3389/fgene.2018.00415] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/30/2018] [Accepted: 09/06/2018] [Indexed: 12/17/2022] Open

Beauséjour M, Boutin A, Vachon PH. Anoikis Regulation: Complexities, Distinctions, and Cell Differentiation. APOPTOSIS AND BEYOND 2018:145-182. [DOI: 10.1002/9781119432463.ch8] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/02/2025]

Singh V, Erady C, Balasubramanian N. Cell-matrix adhesion controls Golgi organization and function through Arf1 activation in anchorage-dependent cells. J Cell Sci 2018;131:jcs.215855. [PMID: 30054383 PMCID: PMC6127727 DOI: 10.1242/jcs.215855] [Citation(s) in RCA: 20] [Impact Index Per Article: 2.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/22/2018] [Accepted: 06/27/2018] [Indexed: 12/15/2022] Open

Haque MR, Jeong JH, Lee KW, Shin DY, Kim GS, Kim SJ, Byun Y. Effects of Transplanted Islets Nano-Encapsulated with Hyperbranched Polyethylene Glycol and Heparin on Microenvironment Reconstruction and Glucose Control. Bioconjug Chem 2018;29:2945-2953. [PMID: 29985588 DOI: 10.1021/acs.bioconjchem.8b00364] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/08/2023]

Affiliation(s)

Muhammad R Haque
Jee-Heon Jeong College of Pharmacy , Yeungnam University , Gyeongsan , Gyeongbuk 712-749 , Republic of Korea
Kyo Won Lee Transplantation Research Center , Samsung Biomedical Research Institute , 81 Ilwon-ro , Gangnam-gu, Seoul 06351 , Republic of Korea.,Department of Surgery, Samsung Medical Center , Sungkyunkwan University School of Medicine , Seoul 06351 , Republic of Korea
Du Yeon Shin Transplantation Research Center , Samsung Biomedical Research Institute , 81 Ilwon-ro , Gangnam-gu, Seoul 06351 , Republic of Korea.,Department of Surgery, Samsung Medical Center , Sungkyunkwan University School of Medicine , Seoul 06351 , Republic of Korea.,Stem Cell & Regenerative Medicine Institute , Samsung Medical Center , Seoul 06351 , Republic of Korea.,Department of Health Sciences & Technology, Samsung Advanced Institute for Health Sciences & Technology, Graduate School , Sungkyunkwan University , Seoul 06351 , Republic of Korea
Geun-Soo Kim Transplantation Research Center , Samsung Biomedical Research Institute , 81 Ilwon-ro , Gangnam-gu, Seoul 06351 , Republic of Korea.,Department of Surgery, Samsung Medical Center , Sungkyunkwan University School of Medicine , Seoul 06351 , Republic of Korea.,Stem Cell & Regenerative Medicine Institute , Samsung Medical Center , Seoul 06351 , Republic of Korea.,Department of Health Sciences & Technology, Samsung Advanced Institute for Health Sciences & Technology, Graduate School , Sungkyunkwan University , Seoul 06351 , Republic of Korea
Sung Joo Kim Transplantation Research Center , Samsung Biomedical Research Institute , 81 Ilwon-ro , Gangnam-gu, Seoul 06351 , Republic of Korea.,Department of Surgery, Samsung Medical Center , Sungkyunkwan University School of Medicine , Seoul 06351 , Republic of Korea.,Stem Cell & Regenerative Medicine Institute , Samsung Medical Center , Seoul 06351 , Republic of Korea.,Department of Health Sciences & Technology, Samsung Advanced Institute for Health Sciences & Technology, Graduate School , Sungkyunkwan University , Seoul 06351 , Republic of Korea
Youngro Byun

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Jia J, Zhang T, Chi J, Liu X, Sun J, Xie Q, Peng S, Li C, Yi L. Neuroprotective Effect of CeO₂@PAA-LXW7 Against H₂O₂-Induced Cytotoxicity in NGF-Differentiated PC12 Cells. Neurochem Res 2018;43:1439-1453. [PMID: 29882125 DOI: 10.1007/s11064-018-2559-y] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/22/2018] [Revised: 05/19/2018] [Accepted: 05/23/2018] [Indexed: 01/12/2023]

Abstract

CeO₂ nanoparticles (nanoceria) have been used in many studies as a powerful free radical scavenger, and LXW7, a small-molecule peptide, can specifically target the integrin αvβ3, whose neuroprotective effects have also been demonstrated. The objective of this study is to observe the neuroprotective effect and potential mechanism of CeO₂@PAA-LXW7, a new compound that couples CeO₂@PAA (nanoceria modified with the functional group of polyacrylic acid) with LXW7 via a series of chemical reactions, in H₂O₂-induced NGF-differentiated PC12 cells. We examined the effects of LXW7, CeO₂@PAA, and CeO₂@PAA-LXW7 on the viability of primary hippocampal neurons and found that there was no significant difference under control conditions, but increased cellular viability was observed in the case of H₂O₂-induced injury. We used H₂O₂-induced NGF-differentiated PC12 cells as the classical injury model to investigate the neuroprotective effect of CeO₂@PAA-LXW7. In this study, LXW7, CeO₂@PAA, and CeO₂@PAA-LXW7 inhibit H₂O₂-induced oxidative stress by reducing the production of reactive oxygen species (ROS) and regulating Bax/Bcl-2, cleaved caspase-3 and mitochondrial cytochrome C (cyto C) in the apoptotic signaling pathways. We found that the levels of phosphorylation of focal adhesion kinase (FAK) and of signal transducer and activator of transcription 3 (STAT3) increased significantly in H₂O₂-induced NGF-differentiated PC12 cells, whereas LXW7, CeO₂@PAA, and CeO₂@PAA-LXW7 suppressed the increase to different degrees. Among the abovementioned changes, the inhibitory effect of CeO₂@PAA-LXW7 on H₂O₂-induced changes, including the increases in the levels of p-FAK and p-STAT3, is more obvious than that of LXW7 or CeO₂@PAA alone. In summary, these results suggest that integrin signaling participates in the regulation of apoptosis via the regulation of ROS and of the apoptosis pathway in H₂O₂-induced NGF-differentiated PC12 cells. LXW7, CeO₂@PAA, and CeO₂@PAA-LXW7 can play neuroprotective roles by counteracting the oxidative stress and apoptosis induced by H₂O₂ in NGF-differentiated PC12 cells. CeO₂@PAA-LXW7 exerting a more powerful synergistic effect via the conjunction of LXW7 and CeO₂@PAA.

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Farberov S, Meidan R. Fibroblast growth factor-2 and transforming growth factor-beta1 oppositely regulate miR-221 that targets thrombospondin-1 in bovine luteal endothelial cells. Biol Reprod 2017;98:366-375. [DOI: 10.1093/biolre/iox167] [Citation(s) in RCA: 16] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/07/2017] [Accepted: 12/05/2017] [Indexed: 12/20/2022] Open

Zhang K, Tan J, Su J, Liang H, Shen L, Li C, Pan G, Yang L, Cui H. Integrin β3 plays a novel role in innate immunity in silkworm, Bombyx mori. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 2017;77:307-317. [PMID: 28826989 DOI: 10.1016/j.dci.2017.08.009] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/21/2017] [Revised: 08/16/2017] [Accepted: 08/17/2017] [Indexed: 06/07/2023]

Guerrero PA, McCarty JH. Integrins in Vascular Development and Pathology. ADVANCES IN PHARMACOLOGY 2017;81:129-153. [PMID: 29310797 DOI: 10.1016/bs.apha.2017.08.011] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 02/16/2023]

A pH-dependent Antibacterial Peptide Release Nano-system Blocks Tumor Growth in vivo without Toxicity. Sci Rep 2017;7:11242. [PMID: 28894233 PMCID: PMC5593885 DOI: 10.1038/s41598-017-11687-y] [Citation(s) in RCA: 18] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/07/2017] [Accepted: 08/30/2017] [Indexed: 12/29/2022] Open

Visavadiya NP, Keasey MP, Razskazovskiy V, Banerjee K, Jia C, Lovins C, Wright GL, Hagg T. Integrin-FAK signaling rapidly and potently promotes mitochondrial function through STAT3. Cell Commun Signal 2016;14:32. [PMID: 27978828 PMCID: PMC5159999 DOI: 10.1186/s12964-016-0157-7] [Citation(s) in RCA: 62] [Impact Index Per Article: 6.9] [Reference Citation Analysis] [Abstract] [Key Words] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/02/2016] [Accepted: 12/06/2016] [Indexed: 01/02/2023] Open

Abstract

Background

STAT3 is increasingly becoming known for its non-transcriptional regulation of mitochondrial bioenergetic function upon activation of its S727 residue (S727-STAT3). Lengthy mitochondrial dysfunction can lead to cell death. We tested whether an integrin-FAK-STAT3 signaling pathway we recently discovered regulates mitochondrial function and cell survival, and treatments thereof.

Methods

Cultured mouse brain bEnd5 endothelial cells were treated with integrin, FAK or STAT3 inhibitors, FAK siRNA, as well as integrin and STAT3 activators. STAT3 null cells were transfected with mutant STAT3 plasmids. Outcome measures included oxygen consumption rate for mitochondrial bioenergetics, Western blotting for protein phosphorylation, mitochondrial membrane potential for mitochondrial integrity, ROS production, and cell counts.

Results

Vitronectin-dependent mitochondrial basal respiration, ATP production, and maximum reserve and respiratory capacities were suppressed within 4 h by RGD and αvβ3 integrin antagonist peptides. Conversely, integrin ligands vitronectin, laminin and fibronectin stimulated mitochondrial function. Pharmacological inhibition of FAK completely abolished mitochondrial function within 4 h while FAK siRNA treatments confirmed the specificity of FAK signaling. WT, but not S727A functionally dead mutant STAT3, rescued bioenergetics in cells made null for STAT3 using CRISPR-Cas9. STAT3 inhibition with stattic in whole cells rapidly reduced mitochondrial function and mitochondrial pS727-STAT3. Stattic treatment of isolated mitochondria did not reduce pS727 whereas more was detected upon phosphatase inhibition. This suggests that S727-STAT3 is activated in the cytoplasm and is short-lived upon translocation to the mitochondria. FAK inhibition reduced pS727-STAT3 within mitochondria and reduced mitochondrial function in a non-transcriptional manner, as shown by co-treatment with actinomycin. Treatment with the small molecule bryostatin-1 or hepatocyte growth factor (HGF), which indirectly activate S727-STAT3, preserved mitochondrial function during FAK inhibition, but failed in the presence of the STAT3 inhibitor. FAK inhibition induced loss of mitochondrial membrane potential, which was counteracted by bryostatin, and increased superoxide and hydrogen peroxide production. Bryostatin and HGF reduced the substantial cell death caused by FAK inhibition over a 24 h period.

Conclusion

These data suggest that extracellular matrix molecules promote STAT3-dependent mitochondrial function and cell survival through integrin-FAK signaling. We furthermore show a new treatment strategy for cell survival using S727-STAT3 activators.

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McKayed K, Prendergast PJ, Campbell VA. Aging enhances the vulnerability of mesenchymal stromal cells to uniaxial tensile strain-induced apoptosis. J Biomech 2016;49:458-62. [DOI: 10.1016/j.jbiomech.2015.11.053] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/28/2015] [Revised: 11/18/2015] [Accepted: 11/21/2015] [Indexed: 10/22/2022]

Chen H, Wang L, Zhou Z, Hou Z, Liu Z, Wang W, Gao D, Gao Q, Wang M, Song L. The comprehensive immunomodulation of NeurimmiRs in haemocytes of oyster Crassostrea gigas after acetylcholine and norepinephrine stimulation. BMC Genomics 2015;16:942. [PMID: 26576764 PMCID: PMC4650145 DOI: 10.1186/s12864-015-2150-8] [Citation(s) in RCA: 22] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/11/2014] [Accepted: 10/26/2015] [Indexed: 12/19/2022] Open

Abstract

Background

Neural-endocrine-immune (NEI) system is a major modulation network among the nervous, endocrine and immune system and weights greatly in maintaining homeostasis of organisms during stress and infection. Some microRNAs are found interacting with NEI system (designated NeurimmiRs), addressing swift modulations on immune system. The oyster Crassostrea gigas, as an intertidal bivalve, has evolved a primary NEI system. However, the knowledge about NeurimmiRs in oysters remains largely unknown.

Results

Six small RNA libraries from haemocytes of oysters stimulated with acetylcholine (ACh) and norepinephrine (NE) were sequenced to identify neurotransmitter-responsive miRNAs and survey their immunomodulation roles. A total of 331 miRNAs (132 identified in the present study plus 199 identified previously) were subjected to expression analysis, and twenty-one and sixteen of them were found ACh- or NE-responsive, respectively (FDR < 0.05). Meanwhile, 21 miRNAs exhibited different expression pattern after ACh or NE stimulation. Consequently, 355 genes were predicted as putative targets of these neurotransmitter-responsive miRNAs in oyster. Through gene onthology analysis, multiple genes involved in death, immune system process and response to stimulus were annotated to be modulated by NeurimmiRs. Besides, a significant decrease in haemocyte phagocytosis and late-apoptosis or necrosis rate was observed after ACh and NE stimulation (p < 0.05) while early-apoptosis rate remained unchanged.

Conclusions

A comprehensive immune-related network involving PRRs, intracellular receptors, signaling transducers and immune effectors was proposed to be modulated by ACh- and NE-responsive NeurimmiRs, which would be indispensable for oyster haemocytes to respond against stress and infection. Characterization of the NeurimmiRs would be an essential step to understand the NEI system of invertebrate and the adaptation mechanism of oyster.

Electronic supplementary material

The online version of this article (doi:10.1186/s12864-015-2150-8) contains supplementary material, which is available to authorized users.

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Dynamic contact angle analysis of protein adsorption on polysaccharide multilayer's films for biomaterial reendothelialization. BIOMED RESEARCH INTERNATIONAL 2014;2014:679031. [PMID: 25276808 PMCID: PMC4171065 DOI: 10.1155/2014/679031] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 06/11/2014] [Accepted: 07/24/2014] [Indexed: 11/17/2022]

Zhang K, Xu M, Su J, Yu S, Sun Z, Li Y, Zhang W, Hou J, Shang L, Cui H. Characterization and identification of the integrin family in silkworm, Bombyx mori. Gene 2014;549:149-55. [DOI: 10.1016/j.gene.2014.07.060] [Citation(s) in RCA: 20] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/22/2014] [Revised: 07/13/2014] [Accepted: 07/23/2014] [Indexed: 11/29/2022]

Nagaraju GP, Dontula R, El-Rayes BF, Lakka SS. Molecular mechanisms underlying the divergent roles of SPARC in human carcinogenesis. Carcinogenesis 2014;35:967-73. [PMID: 24675529 DOI: 10.1093/carcin/bgu072] [Citation(s) in RCA: 102] [Impact Index Per Article: 9.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/06/2023] Open

Banerjee P, Mehta A, Shanthi C. Investigation into the cyto-protective and wound healing properties of cryptic peptides from bovine Achilles tendon collagen. Chem Biol Interact 2014;211:1-10. [DOI: 10.1016/j.cbi.2014.01.002] [Citation(s) in RCA: 17] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/09/2013] [Revised: 12/03/2013] [Accepted: 01/07/2014] [Indexed: 11/26/2022]

Fogh BS, Multhaupt HAB, Couchman JR. Protein kinase C, focal adhesions and the regulation of cell migration. J Histochem Cytochem 2014;62:172-84. [PMID: 24309511 PMCID: PMC3935447 DOI: 10.1369/0022155413517701] [Citation(s) in RCA: 54] [Impact Index Per Article: 4.9] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/24/2013] [Accepted: 11/21/2013] [Indexed: 12/18/2022] Open

Waxmonsky NC, Conner SD. Αvβ3-integrin-mediated adhesion is regulated through an AAK1L- and EHD3-dependent rapid-recycling pathway. J Cell Sci 2013;126:3593-601. [PMID: 23781025 DOI: 10.1242/jcs.122465] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/20/2022] Open

Faião-Flores F, Suarez JAQ, Soto-Cerrato V, Espona-Fiedler M, Pérez-Tomás R, Maria DA. Bcl-2 family proteins and cytoskeleton changes involved in DM-1 cytotoxic effect on melanoma cells. Tumour Biol 2013;34:1235-43. [PMID: 23341182 DOI: 10.1007/s13277-013-0666-6] [Citation(s) in RCA: 14] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/23/2012] [Accepted: 01/11/2013] [Indexed: 01/08/2023] Open

Abstract

Melanoma is one of the most aggressive types of skin cancer and its incidence rate is still increasing. All existing treatments are minimally effective. Consequently, new therapeutic agents for melanoma treatment should be developed. The DM-1 compound is a curcumin analog that possesses several curcumin characteristics, such as antiproliferative, antitumor, and anti-metastatic properties. The aim of this study was to evaluate the different signaling pathways involved in the cytotoxic effect of DM-1 on melanoma cells. The apoptotic process and cytoskeletal changes were evaluated by immunoblotting and immunofluorescence, respectively, in melanoma cells. After DM-1 treatment, SK-MEL-5 melanoma cells showed actin filament disorganization with spicule formation throughout the cytoskeleton and significant reduction of focal adhesion as well as they were present only at cell extremities, conferring a poor connection between the cell and the substrate. Besides this, there was significant filopodium retraction and loss of typical cytoskeleton scaffold. These modifications contributed to cell detachment followed by cell death. Furthermore, DM-1-induced apoptosis was triggered by multiple Bcl-2 proteins involved in both the extrinsic and the intrinsic apoptotic pathways. SK-MEL-5 cells showed a death mechanism mainly by Bcl-2/Bax ratio decrease, whereas A375 cells presented apoptosis induction by Mcl-1 and Bcl-xL downregulation. In SK-MEL-5 and A375 melanoma cells, there was a significant increase in the active form of caspase 9, and the inactive form of the effector caspase 3 was decreased in both cell lines. Expression of cleaved poly ADP ribose polymerase was increased after DM-1 treatment in these melanoma cell lines, demonstrating that the apoptotic process occurred. Altogether, these data elucidate the cellular and molecular mechanisms involved in the cytotoxicity induced by the antitumor agent DM-1 in melanoma cells.

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Chauffert B, Dimanche-Boitrel MT, Garrido C, Ivarsson M, Martin M, Martin F, Solary E. New insights into the kinetic resistance to anticancer agents. Cytotechnology 2012;27:225-35. [PMID: 19002794 DOI: 10.1023/a:1008025124242] [Citation(s) in RCA: 11] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/05/2023] Open

Dai W, Yang T, Wang Y, Wang X, Wang J, Zhang X, Zhang Q. Peptide PHSCNK as an integrin α5β1 antagonist targets stealth liposomes to integrin-overexpressing melanoma. NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE 2012;8:1152-61. [DOI: 10.1016/j.nano.2012.01.003] [Citation(s) in RCA: 25] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/12/2011] [Revised: 09/17/2011] [Accepted: 01/16/2012] [Indexed: 01/11/2023]

ECM Remodeling in Angiogenesis. ACTA ACUST UNITED AC 2012. [DOI: 10.1007/978-3-642-30856-7_9] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/17/2022]

Qin Y, Stokman G, Yan K, Ramaiahgari S, Verbeek F, de Graauw M, van de Water B, Price LS. cAMP signalling protects proximal tubular epithelial cells from cisplatin-induced apoptosis via activation of Epac. Br J Pharmacol 2012;165:1137-50. [PMID: 21745194 PMCID: PMC3346244 DOI: 10.1111/j.1476-5381.2011.01594.x] [Citation(s) in RCA: 30] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/03/2011] [Revised: 06/03/2011] [Accepted: 06/23/2011] [Indexed: 11/30/2022] Open

Abstract

BACKGROUND AND PURPOSE

Nephrotoxicity is the principal dose-limiting factor for cisplatin chemotherapy and is primarily associated with proximal tubular epithelial cells, including disruption of cell adhesions and induction of apoptosis. Cell adhesion and survival is regulated by, amongst other factors, the small GTPase Rap and its activator, the exchange protein directly activated by cAMP (Epac). Epac is particularly enriched in renal tubule epithelium. This study investigates the cytoprotective effects of cAMP-Epac-Rap signalling in a model of cisplatin-induced renal cell injury.

EXPERIMENTAL APPROACH

The Epac-selective cAMP analogue 8-pCPT-2'-O-Me-cAMP was used to activate the Epac-Rap signalling pathway in proximal tubular epithelial cells. Cells were exposed to cisplatin, in the presence or absence of 8-pCPT-2'-O-Me-cAMP, and nephrotoxicity was determined by monitoring cell-cell junctions and cell apoptosis.

KEY RESULTS

Activation of Epac-Rap signalling preserves cell-cell junctions and protects against cell apoptosis of mouse proximal tubular cells during cisplatin treatment. Activation with the Epac-selective cAMP analogue 8-pCPT-2'-O-Me-cAMP or receptor-mediated induction of cAMP both induced cytoprotection against cisplatin, whereas a PKA-selective cAMP analogue was not cytoprotective. 8-pCPT-2'-O-Me-cAMP mediated cytoprotection was blocked by RNAi-mediated silencing of Epac-Rap signalling in these cells. In contrast, 8-pCPT-2'-O-Me-cAMP did not protect against cisplatin-induced cell death of cancer cells that lacked Epac1 expression.

CONCLUSIONS AND IMPLICATIONS

Our study identifies activation of Epac-Rap signalling as a potential strategy for reducing the nephrotoxicity associated with cisplatin treatments and, as a result, broadens the therapeutic window of this chemotherapeutic agent.

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Efficient formation of cell spheroids using polymer nanofibers. Biotechnol Lett 2011;34:795-803. [DOI: 10.1007/s10529-011-0836-9] [Citation(s) in RCA: 16] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/19/2011] [Accepted: 12/19/2011] [Indexed: 10/14/2022]

Collagen VI ablation retards brain tumor progression due to deficits in assembly of the vascular basal lamina. THE AMERICAN JOURNAL OF PATHOLOGY 2011;180:1145-1158. [PMID: 22200614 DOI: 10.1016/j.ajpath.2011.11.006] [Citation(s) in RCA: 37] [Impact Index Per Article: 2.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/20/2010] [Revised: 10/25/2011] [Accepted: 11/08/2011] [Indexed: 12/27/2022]

Yoo BH, Wang Y, Erdogan M, Sasazuki T, Shirasawa S, Corcos L, Sabapathy K, Rosen KV. Oncogenic ras-induced down-regulation of pro-apoptotic protease caspase-2 is required for malignant transformation of intestinal epithelial cells. J Biol Chem 2011;286:38894-903. [PMID: 21903589 DOI: 10.1074/jbc.m111.290692] [Citation(s) in RCA: 17] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/06/2022] Open

Senger DR, Davis GE. Angiogenesis. Cold Spring Harb Perspect Biol 2011;3:a005090. [PMID: 21807843 DOI: 10.1101/cshperspect.a005090] [Citation(s) in RCA: 250] [Impact Index Per Article: 17.9] [Reference Citation Analysis] [Abstract] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/21/2022]

Tissue inhibitor of metalloproteinase 1 expression associated with gene demethylation confers anoikis resistance in early phases of melanocyte malignant transformation. Transl Oncol 2011;2:329-40. [PMID: 19956395 DOI: 10.1593/tlo.09220] [Citation(s) in RCA: 22] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/13/2009] [Revised: 08/13/2009] [Accepted: 09/03/2009] [Indexed: 11/18/2022] Open

Integrin signaling, cell survival, and anoikis: distinctions, differences, and differentiation. JOURNAL OF SIGNAL TRANSDUCTION 2011;2011:738137. [PMID: 21785723 PMCID: PMC3139189 DOI: 10.1155/2011/738137] [Citation(s) in RCA: 97] [Impact Index Per Article: 6.9] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 09/12/2010] [Revised: 01/06/2011] [Accepted: 03/10/2011] [Indexed: 01/01/2023]

Bcl-xL-inhibitory BH3 mimetics can induce a transient thrombocytopathy that undermines the hemostatic function of platelets. Blood 2011;118:1663-74. [PMID: 21673344 DOI: 10.1182/blood-2011-04-347849] [Citation(s) in RCA: 253] [Impact Index Per Article: 18.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/20/2022] Open

Human liver sinusoidal endothelial cells respond to interaction with Entamoeba histolytica by changes in morphology, integrin signalling and cell death. Cell Microbiol 2011;13:1091-106. [DOI: 10.1111/j.1462-5822.2011.01604.x] [Citation(s) in RCA: 22] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/26/2022]

Li B, Pozzi A, Young PP. TNFalpha accelerates monocyte to endothelial transdifferentiation in tumors by the induction of integrin alpha5 expression and adhesion to fibronectin. Mol Cancer Res 2011;9:702-11. [PMID: 21536688 DOI: 10.1158/1541-7786.mcr-10-0484] [Citation(s) in RCA: 29] [Impact Index Per Article: 2.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/03/2023]

Stokman G, Qin Y, Genieser HG, Schwede F, de Heer E, Bos JL, Bajema IM, van de Water B, Price LS. Epac-Rap signaling reduces cellular stress and ischemia-induced kidney failure. J Am Soc Nephrol 2011;22:859-72. [PMID: 21493776 DOI: 10.1681/asn.2010040423] [Citation(s) in RCA: 37] [Impact Index Per Article: 2.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/03/2022] Open

Koch FP, Wunsch A, Merkel C, Ziebart T, Pabst A, Yekta SS, Blessmann M, Smeets R. The influence of bisphosphonates on human osteoblast migration and integrin aVb3/tenascin C gene expression in vitro. Head Face Med 2011;7:4. [PMID: 21299894 PMCID: PMC3044099 DOI: 10.1186/1746-160x-7-4] [Citation(s) in RCA: 14] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/27/2010] [Accepted: 02/07/2011] [Indexed: 12/27/2022] Open

Abstract

Background

Bisphosphonates are therapeutics of bone diseases, such as Paget's disease, multiple myeloma or osteoclastic metastases. As a severe side effect the bisphosphonate induced osteonecrosis of the jaw (BONJ) often requires surgical treatment and is accompanied with a disturbed wound healing.

Therefore, the influence on adhesion and migration of human osteoblasts (hOB) after bisphosphonate therapy has been investigated by morphologic as well as gene expression methods.

Methods

By a scratch wound experiment, which measures the reduction of defined cell layer gap, the morphology and migration ability of hOB was evaluated. A test group of hOB, which was stimulated by zoledronate 5 × 10^-5M, and a control group of unstimulated hOB were applied. Furthermore the gene expression of integrin aVb3 and tenascin C was quantified by Real-Time rtPCR at 5data points over an experimental period of 14 days. The bisphosphonates zoledronate, ibandronate and clodronate have been compared with an unstimulated hOB control.

Results

After initially identical migration and adhesion characteristics, zoledronate inhibited hOB migration after 50 h of stimulation. The integrinavb3 and tenascin C gene expression was effected by bisphosphonates in a cell line dependent manner with decreased, respectively inconsistent gene expression levels over time. The non-nitrogen containing bisphosphonates clodronate led to decreased gene expression levels.

Conclusion

Bisphosphonates seem to inhibit hOB adhesion and migration. The integrin aVb3 and tenascin C gene expression seem to be dependent on the cell line. BONJ could be enhanced by an inhibition of osteoblast adhesion and migration. The gene expression results, however, suggest a cell line dependent effect of bisphosphonates, which could explain the interindividual differences of BONJ incidences.

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Chen CJ, Tseng MC, Lin HJ, Lin TW, Chen YR. Visual indicator for surfactant abundance in MS-based membrane and general proteomics applications. Anal Chem 2011;82:8283-90. [PMID: 20828166 DOI: 10.1021/ac1017937] [Citation(s) in RCA: 12] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/16/2022]