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Madisch A, Pace F, Menzel D, Funk P, Stracke B, Schön C, Labenz J. Prospective uncontrolled clinical study shows rapid and long lasting relief of heartburn and acid related gastric discomfort with Refluthin. Sci Rep 2025; 15:15896. [PMID: 40335653 PMCID: PMC12059117 DOI: 10.1038/s41598-025-98558-z] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/11/2024] [Accepted: 04/14/2025] [Indexed: 05/09/2025] Open
Abstract
Heartburn and acid regurgitation are main symptoms of gastro-esophageal reflux, a widespread complaint with a significant impact on quality of life (QoL). Refluthin® for Heartburn chewable tablets (Refluthin) are a symptomatic treatment option containing a combination of an antacid (CaCO3, MgCO3) and a polysaccharide-rich extract with mucoprotective substances from Opuntia ficus-indica cladodes. To investigate performance and safety of Refluthin in the rapid and long lasting relief of heartburn and acid related gastric discomfort under practical use conditions, a prospective, clinical, uncontrolled, open-label study was conducted. Adults with symptomatic heartburn, acid regurgitation, and/or recurrent acid related gastric discomfort took one tablet Refluthin up to four times/day as needed for up to 4 weeks. Endpoints were both time to onset and duration of symptom relief; reflux symptom intensity/frequency; global assessments (QoL, performance, satisfaction, usability, tolerability); and safety. 81/100 participants (81%) responded with a first symptom relief within ≤ 20 min in ≥ 50% of the individual applications. Long lasting effects of > 120 min were reported by 83/100 (83%) participants. Significant reductions in heartburn event frequency and intensity were seen within the 4 weeks of intermittent use (p < 0.0001, respectively). Global assessment results and safety-relevant findings were also favorable. Results thus demonstrated a distinct rapid and long lasting symptom relief after intake of Refluthin, with a safe and easy use. The significant reductions in frequency and intensity of heartburn events over time indicate sustained effects under treatment. These long-term effects might be explainable by soothing and protection of the irritated mucous membrane by Refluthin.
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Affiliation(s)
- Ahmed Madisch
- Gastroenterology Center Bethanien, Im Prüfling 21-25, 60389, Frankfurt am Main, Germany.
| | - Fabio Pace
- GI Unit, Bolognini Hospital, Via Paderno 21, 24068, Seriate, Bergamo, Italy
| | - Daniel Menzel
- Nutritional CRO, BioTeSys GmbH, Schelztorstrasse 54-56, 73728, Esslingen, Germany
| | - Petra Funk
- Research and Development, Dr. Willmar Schwabe GmbH & Co. KG, Willmar-Schwabe-Straße 4, 76227, Karlsruhe, Germany
| | - Berenike Stracke
- Global Medical Affairs, Dr. Willmar Schwabe GmbH & Co. KG, Willmar-Schwabe-Straße 4, 76227, Karlsruhe, Germany
| | - Christiane Schön
- Nutritional CRO, BioTeSys GmbH, Schelztorstrasse 54-56, 73728, Esslingen, Germany
| | - Joachim Labenz
- Medical Center, Flughafenstraße 2/2a, 57299, Burbach, Germany
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Salamone FL, Molonia MS, Muscarà C, Saija A, Cimino F, Speciale A. In Vitro Protective Effects of a Standardized Extract of Opuntia ficus-indica (L.) Mill. Cladodes and Olea europaea L. Leaves Against Indomethacin-Induced Intestinal Epithelial Cell Injury. Antioxidants (Basel) 2024; 13:1507. [PMID: 39765835 PMCID: PMC11673993 DOI: 10.3390/antiox13121507] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/28/2024] [Revised: 12/05/2024] [Accepted: 12/09/2024] [Indexed: 01/06/2025] Open
Abstract
Nonsteroidal anti-inflammatory drugs (NSAIDs) can induce serious adverse effects in gastrointestinal (GI) mucosa, increasing intestinal permeability and leading to mitochondrial dysfunction, oxidative stress, apoptosis and inflammation. As proton pump inhibitors are effective in protecting against NSAID-induced gastropathy but not NSAID-induced enteropathy, current research is focused on natural products as protective substances for therapy and prevention of intestinal injury. Herein, through the use of an in vitro model based on intestinal epithelial cell (Caco-2) damage caused by indomethacin (INDO), we examined the protective activity of a commercially available standardized extract (OFI+OE) from Opuntia ficus-indica (L.) Mill. cladodes and Olea europaea L. leaves. Pre-treatment with OFI+OE prevented INDO-induced intestinal epithelial barrier damage, as demonstrated by TEER measurement, fluorescein permeability, and tight junction protein expression. The extract showed positive effects against INDO-induced oxidative stress and correlated activation of apoptosis, decreasing pro-apoptotic markers BAX and Caspase-3 and increasing anti-apoptotic factor Bcl-2. Moreover, the extract inhibited the NF-κB pathway and pro-inflammatory cascade. In conclusion, these data support the use of OFI+OE extract as a natural strategy for therapy and prevention of intestinal mucosal damage, demonstrating its beneficial effects against INDO-induced intestinal damage, through modulation of oxidative, apoptotic, and inflammatory pathways.
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Di Napoli M, Badalamenti N, Castagliuolo G, Merra R, Varcamonti M, Zanfardino A, Bruno M, Sottile F. Chemical composition, antimicrobial, and antioxidant activities of Opuntia stricta (Haw.) Haw. mucilage collected in Sicily, Italy. Nat Prod Res 2024; 38:4077-4085. [PMID: 37909244 DOI: 10.1080/14786419.2023.2272781] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/07/2023] [Revised: 10/05/2023] [Accepted: 10/15/2023] [Indexed: 11/02/2023]
Abstract
In this work the mucilage obtained from the cladodes of a not previously investigated accession of Opuntia stricta (Haw.) Haw. (syn. Opuntia dillenii (Ker-Gawl) Haw), collected in Sicily, was analysed by 13C-NMR. The yield of mucilage extracted from cladodes in aqueous medium was 2.55%. The monosaccharides identified, after acidic hydrolyses of the mucilage, were arabinose (36.48%), galactose (32.31%), xylose (15.33%), glucose (10.45%) and rhamnose (5.40%). The mucilage showed a sufficient antimicrobial activity and excellent antioxidant property.
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Affiliation(s)
- Michela Di Napoli
- Department of Biology, University of Naples Federico II, Naples, Italy
| | - Natale Badalamenti
- Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Palermo, Italy
- NBFC, National Biodiversity Future Center, Palermo, Italy
| | | | - Rosalia Merra
- Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Palermo, Italy
| | - Mario Varcamonti
- Department of Biology, University of Naples Federico II, Naples, Italy
| | - Anna Zanfardino
- Department of Biology, University of Naples Federico II, Naples, Italy
| | - Maurizio Bruno
- Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Palermo, Italy
- Centro Interdipartimentale di Ricerca "Riutilizzo bio-based degli scarti da matrici agroalimentari" (RIVIVE), University of Palermo, Palermo, Italy
| | - Francesco Sottile
- Centro Interdipartimentale di Ricerca "Riutilizzo bio-based degli scarti da matrici agroalimentari" (RIVIVE), University of Palermo, Palermo, Italy
- Department of Architecture, University of Palermo, Palermo, Italy
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Cherrada N, Chemsa A, Gheraissa N, Laib I, Gueboudji Z, EL‐Shazly M, Zaater A, Abid A, Sweilam S, Emran T, Nani S, Benamor B, Ghemam Amara D, Atoki A, Messaoudi M. Gastroprotective Efficacy of North African Medicinal Plants: A Review on Their Therapeutic Potential for Peptic Ulcers. Food Sci Nutr 2024; 12:8793-8824. [PMID: 39619964 PMCID: PMC11606823 DOI: 10.1002/fsn3.4536] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/28/2024] [Revised: 09/15/2024] [Accepted: 09/29/2024] [Indexed: 01/12/2025] Open
Abstract
Peptic ulcer disease remains a prevalent gastrointestinal disorder worldwide. Current treatments often have limitations, sparking interest in alternative therapies from medicinal plants. This review examines the gastroprotective potential of 54 North African medicinal plants against peptic ulcers. An extensive literature search was conducted, focusing on plants with preclinical and clinical evidence of anti-ulcer efficacy and documented use in North African traditional medicine. The review identified several promising plant species, such as licorice (Glycyrrhiza glabra), chamomile (Matricaria chamomilla), olive (Olea europaea), pomegranate (Punica granatum), Aloe vera, and black seed (Nigella sativa), along with their bioactive constituents, including flavonoids, tannins, and terpenoids. These compounds exhibit gastroprotective properties through multiple mechanisms, such as enhancing the gastric mucosal barrier, inhibiting acid secretion, displaying antioxidant and anti-inflammatory effects, promoting ulcer healing, and combating Helicobacter pylori infection. The evidence presented includes in vitro assays, animal models, and some clinical studies. While many of the 53 plants reviewed demonstrated significant anti-ulcer effects compared to standard drugs, further clinical research is needed to establish efficacy and safety in humans. The synergistic actions of phytochemical mixtures in medicinal plant extracts likely contribute to their therapeutic potential. This review highlights the role these North African medicinal plants may play in the prevention and treatment of peptic ulcers and identifies promising candidates for further research and development of evidence-based botanical therapies.
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Affiliation(s)
- Nezar Cherrada
- Faculty of Life and Natural Sciences, Department of BiologyUniversity of El OuedEl‐OuedAlgeria
- Laboratory of Biodiversity and Application of Biotechnology in AgricultureUniversity of El OuedEl‐OuedAlgeria
| | - Ahmed Elkhalifa Chemsa
- Faculty of Life and Natural Sciences, Department of BiologyUniversity of El OuedEl‐OuedAlgeria
- Laboratory of Biodiversity and Application of Biotechnology in AgricultureUniversity of El OuedEl‐OuedAlgeria
| | - Noura Gheraissa
- Faculty of Life and Natural Sciences, Department of BiologyUniversity of El OuedEl‐OuedAlgeria
- Laboratory of Biodiversity and Application of Biotechnology in AgricultureUniversity of El OuedEl‐OuedAlgeria
| | - Ibtissam Laib
- Faculty of Life and Natural Sciences, Department of Molecular and Cellular BiologyUniversity of El OuedEl‐OuedAlgeria
| | - Zakia Gueboudji
- Faculty of Nature and Life SciencesAbbes Laghrour University of KhenchelaKhenchelaAlgeria
- Biotechnology, Water, Environment and Health LaboratoryAbbes Laghrour University of KhenchelaKhenchelaAlgeria
| | - Mohamed EL‐Shazly
- Faculty of Pharmacy, Department of PharmacognosyAin Shams UniversityCairoEgypt
| | - Abdelmalek Zaater
- Laboratory of Biodiversity and Application of Biotechnology in AgricultureUniversity of El OuedEl‐OuedAlgeria
- Faculty of Life and Natural Sciences, Department of AgronomyUniversity of El OuedEl‐OuedAlgeria
| | - Asma Abid
- Faculty of Mathematics and Matter SciencesUniversity of OuarglaOuarglaAlgeria
- Laboratory of Valorization and Promotion of Saharan Resources (VPRS)OuarglaAlgeria
| | - Sherouk Hussein Sweilam
- College of Pharmacy, Department of PharmacognosyPrince Sattam Bin Abdulaziz UniversityAl‐KharjSaudi Arabia
- Faculty of Pharmacy, Department of PharmacognosyCairo‐Suez RoadEgyptian Russian UniversityBadr City, CairoEgypt
| | - Talha Bin Emran
- Warren Alpert Medical School, Department of Pathology and Laboratory MedicineBrown UniversityProvidenceRhode IslandUSA
- Legorreta Cancer CenterBrown UniversityProvidenceRhode IslandUSA
- Faculty of Allied Health Sciences, Department of PharmacyDaffodil International UniversityDhakaBangladesh
| | - Sadok Nani
- Faculty of Life and Natural Sciences, Department of Molecular and Cellular BiologyUniversity of El OuedEl‐OuedAlgeria
| | - Bilal Benamor
- Higher School of Saharan Agriculture‐El OuedEl OuedAlgeria
- Laboratory of Genetic, Biotechnology and Valorization of Bio‐Resources (LGBVB)University of Mohamed KhiderBiskraAlgeria
| | - Djilani Ghemam Amara
- Faculty of Life and Natural Sciences, Department of BiologyUniversity of El OuedEl‐OuedAlgeria
- Laboratory Biology, Environment, and HealthUniversity of El OuedEl‐OuedAlgeria
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Koufan M, Choukrane B, Mazri MA. Structure-Function Relationships and Health-Promoting Properties of the Main Nutraceuticals of the Cactus Pear ( Opuntia spp.) Cladodes: A Review. Molecules 2024; 29:4732. [PMID: 39407660 PMCID: PMC11477999 DOI: 10.3390/molecules29194732] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/30/2024] [Revised: 09/25/2024] [Accepted: 10/05/2024] [Indexed: 10/20/2024] Open
Abstract
Over the past decade, several studies have established a direct link between functional foods, nutraceuticals, and a reduced risk of oxidative-stress-related diseases. Nutraceuticals, which encompass a variety of bioactive molecules, exhibit both nutritional and therapeutic properties. The cactus pear (Opuntia spp.) is a plant genus with many species recognized as functional foods, largely attributed to their high content of nutraceuticals, including polyphenols, fatty acids, vitamins, amino acids, pigments, and phytosterols. These compounds of different structures and functions possess different biological activities, contributing to the health-promoting properties of cactus pear. This makes cactus pears a valuable plant for the food, cosmetic, and pharmaceutical industries. While extensive research has focused on the nutritional profile of cactus pear fruits, the cladodes have received comparatively limited attention. Notably, the nutritional composition of cladodes can exhibit considerable variability, influenced by species and growing conditions. Furthermore, although various bioactive compounds have been identified in cladodes, studies elucidating their mechanisms of action, health benefits, and potential therapeutic applications remain insufficient. Addressing these gaps is crucial for enhancing the understanding and utilization of cactus pear cladodes. This paper provides a comprehensive overview of the structure-function relationships of the main nutraceuticals found in cactus pear cladodes. It synthesizes data from recent and relevant literature to elucidate the content of these compounds in relation to species and geographical origin, while also detailing the main biological activities and health-promoting benefits associated with cactus pear cladodes.
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Affiliation(s)
- Meriyem Koufan
- Natural Resources and Local Products Research Unit, Regional Center of Agricultural Research of Agadir, National Institute of Agricultural Research, Avenue Ennasr, BP 415 Rabat Principale, Rabat 10090, Morocco
| | - Basma Choukrane
- Plant Breeding and Quality Research Unit, Regional Center of Agricultural Research of Marrakech, National Institute of Agricultural Research, Avenue Ennasr, BP 415 Rabat Principale, Rabat 10090, Morocco;
| | - Mouaad Amine Mazri
- Agro-Biotechnology Research Unit, Regional Center of Agricultural Research of Marrakech, National Institute of Agricultural Research, Avenue Ennasr, BP 415 Rabat Principale, Rabat 10090, Morocco
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Lehner MD, Scheyhing U, Elsässer J. A combination of carbonates and Opuntia ficus-indica extract protects esophageal cells against simulated acidic and non-acidic reflux in vitro. Sci Rep 2024; 14:22355. [PMID: 39333650 PMCID: PMC11437097 DOI: 10.1038/s41598-024-74047-7] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/21/2024] [Accepted: 09/23/2024] [Indexed: 09/29/2024] Open
Abstract
Buffering of stomach acid by antacids is a well-established symptomatic therapy for heartburn. In addition, preparations from prickly pear (Opuntia ficus-indica) have been shown to reduce tissue damage in experimental gastritis models and to attenuate gastrointestinal discomfort in patients. Both active principles have been included in a fixed-combination product for symptomatic treatment of heartburn containing carbonate antacids (CaCO3 and MgCO3) and an extract from Opuntia ficus-indica cladodes. The aim of the study was to characterize the acid neutralization and esophageal cell protective activities of the product and its individual active ingredients in a set of in vitro assays. Acid neutralization was assessed in a simulated stomach model. Protective activity of individual constituents and in combination was analyzed in an esophageal cell line (COLO-680 N) exposed to low pH and deoxycholic acid to simulate acidic and non-acidic reflux challenge. The combination product protected cells against low pH mediated cytotoxicity via acid neutralization by carbonates. Opuntia extract itself and the combination product attenuated bile acid-induced cell irritation as measured by reduced release of proinflammatory interleukin-6 and -8. In conclusion, addition of Opuntia extract to a mineral antacid provides dual protection against acidic and non-acidic simulated reflux challenge.
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Affiliation(s)
- Martin D Lehner
- Dr. Willmar Schwabe GmbH & Co. KG, Willmar-Schwabe-Str. 4, 76227, Karlsruhe, Germany.
| | - Ulrike Scheyhing
- Dr. Willmar Schwabe GmbH & Co. KG, Willmar-Schwabe-Str. 4, 76227, Karlsruhe, Germany
| | - Jens Elsässer
- Dr. Willmar Schwabe GmbH & Co. KG, Willmar-Schwabe-Str. 4, 76227, Karlsruhe, Germany
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7
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Bahloul B, Ben Bnina E, Dridi D, Bouhamed A, Castillo Henríquez L, Flamini G, Vega-Baudrit JR. Development and Investigation of a Nanoemulgel Formulated from Tunisian Opuntia ficus-indica L. Seed Oil for Enhanced Wound Healing Activity. Gels 2024; 10:582. [PMID: 39330184 PMCID: PMC11431070 DOI: 10.3390/gels10090582] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/13/2024] [Revised: 08/30/2024] [Accepted: 09/05/2024] [Indexed: 09/28/2024] Open
Abstract
The aim of this study is to develop a nanoemulgel encapsulating a Tunisian Prickly Pear (Opuntia ficus-indica L.) seed oil (PPSO) to assess, for the first time, the in vivo efficacy of this nanoformulation on wound healing. Phytocompounds of this oil have been reported in the literature as having powerful pharmacological activities. However, it remains poorly exploited due to low bioavailability. A nanoemulsion (NE) was designed by determining the required hydrophilic-lipophilic balance (HLB) and subsequently characterized. The mean droplet size was measured at 56.46 ± 1.12 nm, with a polydispersity index (PDI) of 0.23 ± 0.01 using dynamic light scattering. The zeta potential was -31.4 ± 1.4 mV, and the morphology was confirmed and assessed using transmission electron microscopy (TEM). These characteristics align with the typical properties of nanoemulsions. The gelification process resulted in the formation of a nanoemulgel from the optimum nanoemulsion. The high wound healing efficiency of the nanoemulgel was confirmed compared to that of a medicinally marketed cream. The outcomes of this research contribute valuable insights, for the first time, into the potential therapeutic applications of PPSO and its innovative pharmaceutical formulation for wound healing.
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Affiliation(s)
- Badr Bahloul
- Drug Development Laboratory LR12ES09, Faculty of Pharmacy, University of Monastir, Monastir 5000, Tunisia
| | - Enis Ben Bnina
- LR21AGR03-Production and Protection for a Sustainable Horticulture (2PHD), IRESA-University of Sousse, Regional Research Centre on Horticulture and Organic Agriculture, Sousse 4042, Tunisia
| | - Dorra Dridi
- Drug Development Laboratory LR12ES09, Faculty of Pharmacy, University of Monastir, Monastir 5000, Tunisia
| | - Aya Bouhamed
- Drug Development Laboratory LR12ES09, Faculty of Pharmacy, University of Monastir, Monastir 5000, Tunisia
| | - Luis Castillo Henríquez
- CNRS, INSERM, UTCBS, Chemical and Biological Technologies for Health Laboratory, Université Paris Cité, F-75006 Paris, France
| | - Guido Flamini
- Dipartimento di Farmacia, Via Bonanno 6, 56126 Pisa, Italy
| | - José Roberto Vega-Baudrit
- National Nanotechnology Laboratory (LANOTEC), National Center for High Technology (CeNAT), San José 1174-1200, Costa Rica
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Vranová V. Comprehensive therapy of upper functional dyspepsia: the potential of herbal medicines. CESKA A SLOVENSKA FARMACIE : CASOPIS CESKE FARMACEUTICKE SPOLECNOSTI A SLOVENSKE FARMACEUTICKE SPOLECNOSTI 2024; 73:105-109. [PMID: 40035301 DOI: 10.36290/csf.2024.012] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 03/05/2025]
Abstract
Comprehensive therapy of upper functional dyspepsia: the potential of herbal medicines Functional gastrointestinal disorders are among the most common diseases. The treatment of these conditions must be comprehensive. The foundation of therapy includes lifestyle modifications, appropriate dietary adjustments, physiotherapy, and pharmacotherapy. Herbal medicines can also play a significant role in self-treatment.
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Reyes Escogido MDL, Barrón Vilchis D, Zavala Martínez LG, Angulo Romero F. Opuntia robusta mucilage combined with alginate as encapsulation matrix for Lactiplantibacillus plantarum. CYTA - JOURNAL OF FOOD 2023. [DOI: 10.1080/19476337.2023.2168303] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 02/04/2023]
Affiliation(s)
| | - Daniela Barrón Vilchis
- Department of Pharmacy, Division of Natural and Exact Sciences, University of Guanajuato, Guanajuato, Mexico
| | | | - Fabiola Angulo Romero
- Department of Medicine and Nutrition, Health Sciences Division, University of Guanajuato, Leon, Mexico
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Demir D, Kabak S, Çağlayan K. Purification and Characterization of Polyphenol Oxidase in the Fruits of Opuntia ficus-indica. BIOLOGY 2023; 12:1339. [PMID: 37887049 PMCID: PMC10604213 DOI: 10.3390/biology12101339] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/12/2023] [Revised: 10/10/2023] [Accepted: 10/12/2023] [Indexed: 10/28/2023]
Abstract
Firstly, polyphenol oxidase (PPO) was purified from the fruits of Opuntia ficus-indica using Sepharose 4B-L-tyrosine-p-aminobenzoic acid affinity chromatography, and the enzyme was characterized. The PPO was purified 20.59-fold. Thereafter, PPO was performed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The kinetic parameters, optimum pHs, and optimum temperatures were investigated for three substrates. Opuntia ficus-indica PPO's optimum pH and optimum temperature were 9.0 and 20 °C; 7.5 and 20 °C; and 7.5 and 30 °C, respectively, when using pyrogallol, catechol, and 4-methyl catechol as substrates. For the pyrogallol, catechol, and 4-methyl catechol, the Km, Vmax, and Vmax/Km values were determined as 16.67 mM, 833.33 U/mLmin, and 50 U/mLminmM; 6.33 mM, 126.58 U/mLmin, and 20 U/mLminmM; and 5.38 mM, 107.53 U/mLmin, and 20 U/mLminmM, respectively. As a result, pyrogallol was a more appropriate substrate than catechol and 4-methyl catechol for the PPO from Opuntia ficus-indica.
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Affiliation(s)
- Dudu Demir
- Department of Agricultural Biotechnology, Faculty of Agriculture, Isparta University of Applied Sciences, 32260 Isparta, Türkiye; (S.K.); (K.Ç.)
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Nova ICV, de Almeida WA, Procópio TF, Godoy RSM, Miranda FR, Barbosa RC, Nascimento JDS, Paiva PMG, Ferreira MRA, Soares LAL, Pimenta PFP, Martins GF, Navarro DMDAF, Napoleão TH, Pontual EV. Extract from Opuntia ficus-indica cladode delays the Aedes aegypti larval development by inducing an axenic midgut environment. ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY 2023; 113:e21872. [PMID: 35112391 DOI: 10.1002/arch.21872] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/14/2021] [Revised: 12/31/2021] [Accepted: 01/13/2022] [Indexed: 06/14/2023]
Abstract
This study evaluated the effects of acute exposure of Aedes aegypti third instar (L3 ) larvae to the saline extract of Opuntia ficus-indica cladodes on the biological cycle and fertility of the emerging adults. For this, larvae were treated for 24 h with the extract at ¼ LC50 (lethal concentration to kill 50% of larvae), ½ LC50 or LC50 ; the development and reproduction of the emerged adults were evaluated after a recovery period of 9 days. The resistance of proteins in the extract to hydrolysis by L3 digestive enzymes and histomorphological alterations in the larval midgut were also investigated. The extract contained lectin, flavonoids, cinnamic derivatives, terpenes, steroids, and reducing sugars. It showed a LC50 of 3.71% for 48 h. The data indicated mean survival times similar in control and extract treatments. It was observed development delay in extract-treated groups, with a lower number of adults than in control. However, the females that emerged laid similar number of eggs in control and treatments. Histological evaluation revealed absence of bacterial and fungal microorganisms in the food content in midguts from larvae treated with cladode extract. Electrophoresis revealed that three polypeptides in the extract resisted to hydrolysis by L3 digestive proteases for 90 min. The lectin activity was not altered even after 24-h incubation with the enzymes. In conclusion, the extract from O. ficus-indica can delay the development of Ae. aegypti larvae, which may be linked to induction of an axenic environment at larval midgut and permanence of lectin activity even after proteolysis.
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Affiliation(s)
- Isabella C V Nova
- Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil
| | - Welton A de Almeida
- Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Recife, Brazil
| | - Thamara F Procópio
- Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil
| | - Raquel S M Godoy
- Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Brazil
- Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, Brazil
| | - Franciane R Miranda
- Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Brazil
| | - Renata C Barbosa
- Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Brazil
| | - Jéssica da Silva Nascimento
- Departamento de Química Fundamental, Centro de Ciências Exatas e da Natureza, Universidade Federal Rural de Pernambuco, Recife, Brazil
| | - Patrícia M G Paiva
- Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil
| | - Magda R A Ferreira
- Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, Recife, Brazil
| | - Luiz A L Soares
- Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, Recife, Brazil
| | - Paulo F P Pimenta
- Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Brazil
| | - Gustavo F Martins
- Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Brazil
| | - Daniela Maria do Amaral F Navarro
- Departamento de Química Fundamental, Centro de Ciências Exatas e da Natureza, Universidade Federal Rural de Pernambuco, Recife, Brazil
| | - Thiago H Napoleão
- Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil
| | - Emmanuel V Pontual
- Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Recife, Brazil
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Martins M, Ribeiro MH, Almeida CMM. Physicochemical, Nutritional, and Medicinal Properties of Opuntia ficus-indica (L.) Mill. and Its Main Agro-Industrial Use: A Review. PLANTS (BASEL, SWITZERLAND) 2023; 12:1512. [PMID: 37050137 PMCID: PMC10096643 DOI: 10.3390/plants12071512] [Citation(s) in RCA: 16] [Impact Index Per Article: 8.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 03/08/2023] [Revised: 03/27/2023] [Accepted: 03/28/2023] [Indexed: 06/19/2023]
Abstract
The cactus, Opuntia ficus-indica (L.) Mill. (OFI) belongs to the Cactaceae family, which contains about 130 genera and nearly 1600 species. This review aims to evaluate this plant from several perspectives, namely, botanic, physicochemical, nutritional, and medicinal properties, as well as agro-industrial use. The botanical aspects and morphological characteristics of OFI enable genetic variability, ecological adaptation, and broad geographic distribution. Due to its physicochemical and nutritional composition, it has several medicinal properties appropriate (or suitable) for several industries, such as pharmaceutical, food, and cosmetics. Its fruit, the prickly pear (PP), has potential agro-industrial expansion through the application of different conservation and transformation methods, making it possible to obtain a variety of products. The PP is a source of several nutrients and is an effective system to produce varied foods, which have several advantages from a nutritional, sensory, economic, and shelf-life point of view.
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Affiliation(s)
- Mariana Martins
- Laboratory of Bromatology and Water Quality, Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, 2, 1649-003 Lisboa, Portugal;
| | - Maria H. Ribeiro
- Research Institute for Medicines (iMed), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal;
| | - Cristina M. M. Almeida
- Laboratory of Bromatology and Water Quality, Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, 2, 1649-003 Lisboa, Portugal;
- Research Institute for Medicines (iMed), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal;
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13
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Bioprospecting and potential of cactus mucilages: A bibliometric review. Food Chem 2023; 401:134121. [DOI: 10.1016/j.foodchem.2022.134121] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/01/2022] [Revised: 08/19/2022] [Accepted: 09/02/2022] [Indexed: 11/22/2022]
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Madrigal-Santillán E, Portillo-Reyes J, Madrigal-Bujaidar E, Sánchez-Gutiérrez M, Izquierdo-Vega JA, Izquierdo-Vega J, Delgado-Olivares L, Vargas-Mendoza N, Álvarez-González I, Morales-González Á, Morales-González JA. Opuntia spp. in Human Health: A Comprehensive Summary on Its Pharmacological, Therapeutic and Preventive Properties. Part 2. PLANTS (BASEL, SWITZERLAND) 2022; 11:2333. [PMID: 36145735 PMCID: PMC9505094 DOI: 10.3390/plants11182333] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 07/27/2022] [Revised: 08/18/2022] [Accepted: 08/31/2022] [Indexed: 11/16/2022]
Abstract
Plants of the genus Opuntia spp are widely distributed in Africa, Asia, Australia and America. Specifically, Mexico has the largest number of wild species; mainly O. streptacantha, O. hyptiacantha, O. albicarpa, O. megacantha and O. ficus-indica. The latter being the most cultivated and domesticated species. Its main bioactive compounds include pigments (carotenoids, betalains and betacyanins), vitamins, flavonoids (isorhamnetin, kaempferol, quercetin) and phenolic compounds. Together, they favor the different plant parts and are considered phytochemically important and associated with control, progression and prevention of some chronic and infectious diseases. Part 1 collected information on its preventive actions against atherosclerotic cardiovascular diseases, diabetes and obesity, hepatoprotection, effects on human infertility and chemopreventive capacity. Now, this second review (Part 2), compiles the data from published research (in vitro, in vivo, and clinical studies) on its neuroprotective, anti-inflammatory, antiulcerative, antimicrobial, antiviral potential and in the treatment of skin wounds. The aim of both reviews is to provide scientific evidences of its beneficial properties and to encourage health professionals and researchers to expand studies on the pharmacological and therapeutic effects of Opuntia spp.
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Affiliation(s)
- Eduardo Madrigal-Santillán
- Escuela Superior de Medicina, Instituto Politécnico Nacional, “Unidad Casco de Santo Tomas”, Ciudad de México 11340, Mexico
| | - Jacqueline Portillo-Reyes
- Escuela Superior de Medicina, Instituto Politécnico Nacional, “Unidad Casco de Santo Tomas”, Ciudad de México 11340, Mexico
| | - Eduardo Madrigal-Bujaidar
- Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, “Unidad Profesional A. López Mateos”, Ciudad de México 07738, Mexico
| | - Manuel Sánchez-Gutiérrez
- Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Ex-Hacienda de la Concepción, Tilcuautla, Pachuca de Soto 42080, Mexico
| | - Jeannett A. Izquierdo-Vega
- Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Ex-Hacienda de la Concepción, Tilcuautla, Pachuca de Soto 42080, Mexico
| | - Julieta Izquierdo-Vega
- Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Ex-Hacienda de la Concepción, Tilcuautla, Pachuca de Soto 42080, Mexico
| | - Luis Delgado-Olivares
- Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Ex-Hacienda de la Concepción, Tilcuautla, Pachuca de Soto 42080, Mexico
| | - Nancy Vargas-Mendoza
- Escuela Superior de Medicina, Instituto Politécnico Nacional, “Unidad Casco de Santo Tomas”, Ciudad de México 11340, Mexico
| | - Isela Álvarez-González
- Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, “Unidad Profesional A. López Mateos”, Ciudad de México 07738, Mexico
| | - Ángel Morales-González
- Escuela Superior de Cómputo, Instituto Politécnico Nacional, “Unidad Profesional A. López Mateos”, Ciudad de México 07738, Mexico
| | - José A. Morales-González
- Escuela Superior de Medicina, Instituto Politécnico Nacional, “Unidad Casco de Santo Tomas”, Ciudad de México 11340, Mexico
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Awobajo FO, Sofidiya MO, Asekun OT, Familoni BO. Acute and sub-chronic toxicity assessment and evaluation of the gastro-protective activity of polyherbal formulation “Mystomate4®” against gastric ulcer in experimental laboratory animal. CLINICAL PHYTOSCIENCE 2022. [DOI: 10.1186/s40816-022-00343-2] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 11/10/2022] Open
Abstract
Abstract
Background
Ulcer remains a health challenge worldwide with antibiotics and proton pump inhibitors as major management therapy. The study investigated the acute, sub-chronic toxicity and gastrointestinal protective activity of a polyherbal formulation (Mystomate4®) used locally in Nigeria.
Methods
Oral LD50 and the sub-chronic toxicity test were determined in mice and rats. Mice were grouped into 8 groups of 8 mice each. They were dosed a graded concentration of the formulation (1.28, 2.56; 5.12; 10.24; 20.48; 40.96; 81.92; 163.84 g/kg body weight). The graded dose used was arrived at after an initial pilot study. Thereafter doses were chosen around the dose obtained from the pilot study. Animal were dosed orally and observed for sign of toxicity and number of death recorded after 24 h. The sub-chronic toxicity study was carried out for 3 months in rats at a dose of 2.5 and 5.0 g/kg body weight arrived at by titrating down the LD50 value after which some vital tissues were harvested and assessed for toxicity using relevant biomarkers. Anti-ulcer activity was evaluated in rats using ethanol, indomethacin and pylorus ligation induced ulcer models. Data were analysed with Graph Pad Prism version 5.0 using appropriate statistical method and significant level placed at p ≤ 0.05.
Results
The acute toxicity study showed an LD50 result of 22,837.21 g/kg. The sub-chronic toxicity study resulted in a significant reduction in body weight due to significant decrease (p ≤ 0.05) in feed consumption. Biochemical analyses of the blood samples showed a significant increase (p ≤ 0.05) in creatinine and albumin level in the 2.5 mg/kg female group. ALT was significantly increased in all the treated rats except in 2 mg/kg female rats. Alkaline phosphatase significantly increased in high dosed male (HM) group while blood urea:creatinine ratio was significantly lowered in all the treated groups. There was a significant increase in serum TGL in all rats while LDL was significantly increased and decreased in HM and high dosed female (HF) respectively.
Conclusion
Mystomate4® showed significant protection against ethanol and indomethacin-induced ulcer models but did not modify the gastric parameters in pylorus ligation-induced ulcer model. The polyherbal formulation is nontoxic with promising potentials for treating experimental peptic ulcer.
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Kadda S, Belabed A, Loukili EH, Hammouti B, Fadlaoui S. Temperature and extraction methods effects on yields, fatty acids, and tocopherols of prickly pear (Opuntia ficus-indica L.) seed oil of eastern region of Morocco. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH INTERNATIONAL 2022; 29:158-166. [PMID: 34648165 DOI: 10.1007/s11356-021-16752-8] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/29/2021] [Accepted: 09/22/2021] [Indexed: 06/13/2023]
Abstract
The present study focuses on the effect of temperature and extraction methods on the yields, chemical quality, fatty acids, and tocopherols of the oil extracted from the seeds of Opuntia ficus-indica, collected in the eastern region of Morocco. Our results revealed the effect of temperature that when we increase the temperature used, the yields also increase; the results also showed that this high temperature does not affect the physicochemical properties, fatty acids, and tocopherols. Thus, the results of this study revealed that the prickly pear is a rich source of oil; the obtained oil yields varied from 12.49%±0.09 for mechanical extraction, 11.46±0.10 for chemical extraction, and 10.52%±0.09 for maceration. The main fatty acids founded in Opuntia ficus-indica are linoleic acid 75.80%±0.10 (chemical), 74.07%±0.14 (maceration), and 71.59%±0.14 (mechanical) and palmitic acid 17.32%±0.02 (chemical), 22.419%±0.06 (maceration), and 26.58%±0.00 (mechanical); prickly pear oil could be classified as a linoleic acid. The physicochemical properties of Opuntia ficus-indica seed oils such as acid index mgKOH/g oil (4,376±0.10, 5.854±0.03, 5.667±0.07), saponification value mgKOH/g oil (181.12 ±0.18, 183.77±1.23, 179.08±3.45), and peroxide value 20milieq/Kg (5.75±0.08, 6±0.06, 5.97±0.04) for mechanical, chemical, and maceration extraction, respectively, density, and refractive index were all found to be in good accordance with quality criteria for both pure and fresh oils. Among the tocopherols found, a high value of γ-tocopherol was detected in mechanical extraction with 502.04±0.76 mg/kg, followed by chemical extraction and maceration with 430.12±0.61mg/kg and 315.47±0.96 mg/kg, respectively.
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Affiliation(s)
- Salma Kadda
- Faculty of Sciences, Department of Biology, Laboratory of Improvement of Agricultural Production, Biotechnology and Environment, Mohamed First University, Oujda, Morocco.
| | - Abdelmadjid Belabed
- Faculty of Sciences, Department of Biology, Laboratory of Improvement of Agricultural Production, Biotechnology and Environment, Mohamed First University, Oujda, Morocco
| | - El Hassania Loukili
- Faculty of Sciences, Department of Chemistry, Laboratory of Applied Chemistry, Materials and Environment, Mohamed First University, Oujda, Morocco
| | - Belkheir Hammouti
- Faculty of Sciences, Department of Chemistry, Laboratory of Applied Chemistry, Materials and Environment, Mohamed First University, Oujda, Morocco
| | - Soufiane Fadlaoui
- Faculty of Sciences, Department of Biology, Laboratory of Improvement of Agricultural Production, Biotechnology and Environment, Mohamed First University, Oujda, Morocco
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Kumadoh D, Archer MA, Yeboah GN, Kyene MO, Boakye-Yiadom M, Adi-Dako O, Osei-Asare C, Adase E, Appiah AA, Mintah SO. A review on anti-peptic ulcer activities of medicinal plants used in the formulation of Enterica, Dyspepsia and NPK 500 capsules. Heliyon 2021; 7:e08465. [PMID: 34917789 PMCID: PMC8645450 DOI: 10.1016/j.heliyon.2021.e08465] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/09/2021] [Revised: 09/10/2021] [Accepted: 11/19/2021] [Indexed: 11/03/2022] Open
Abstract
Peptic ulcer disease affects many people globally. With the increasing resistance to some orthodox antibiotics such as Clarithromycin and Metronidazole, it is important that new acceptable, safer and effective therapies are developed to manage this disease. Various herbal medicines have been used traditionally for the remedy of peptic ulcer disease (PUD), however scientific information with regards to their anti-peptic ulcer both in-vivo and in-vitro as well as clinical studies supporting their use is still inadequate. The Centre for Plant Medicine Research, (CPMR) Mampong-Akuapem, Ghana manufactures three herbal Products namely Enterica, Dyspepsia and NPK 500 capsules which are currently used for the remedy of PUD as a triple therapy at its out-patient clinic with promising effects. The aim of this review is to gather information from literature on the anti-ulcer properties, pharmacological, phytochemical constituents and related activities of herbal plants used at the CPMR for formulation of the triple herbal therapy. This review may, provide some scientific bases for the use of Enterica, Dyspepsia and NPK 500 capsules in the management of Peptic ulcer at the CPMR out-patient clinic. METHODS Organization for the review involved the on and/or offline search for information from available literature using electronic data and scientific research information resources such as PubMed, Science Direct and Google scholar. RESULTS In this review, fifteen ethno-medicinal plants used for the formulation of Enterica, Dyspepsia and NPK capsules have been discussed, presenting the description of the plants, composition and pharmacological activity. INTERPRETATION Tables with the summary of reviewed medicinal plants with their anti-ulcer models and inference on possible mechanisms of action were drawn up. The mechanism(s) of action of individual plants and products (Enterica, Dyspepsia and NPK 500 capsules) must be further investigated and established experimentally in-vitro in addition to in-vivo pharmacological and clinical activity studies to confirm their use in the remedy of PUD.
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Affiliation(s)
- Doris Kumadoh
- Department of Pharmaceutics, Centre for Plant Medicine Research, Mampong-Akuapem, Ghana
- Department of Production, Centre for Plant Medicine Research, Mampong-Akuapem, Ghana
| | - Mary-Ann Archer
- Department of Pharmaceutics, Centre for Plant Medicine Research, Mampong-Akuapem, Ghana
| | - Genevieve N. Yeboah
- Department of Pharmaceutics, Centre for Plant Medicine Research, Mampong-Akuapem, Ghana
| | - Michael O. Kyene
- Department of Pharmaceutics, Centre for Plant Medicine Research, Mampong-Akuapem, Ghana
| | - Mavis Boakye-Yiadom
- Department of Clinical Research, Centre for Plant Medicine Research, Mampong-Akuapem, Ghana
| | - Ofosua Adi-Dako
- Department of Pharmaceutics and Microbiology, School of Pharmacy, University of Ghana, Legon, Accra, Ghana
| | - Christina Osei-Asare
- Department of Pharmaceutics and Microbiology, Central University College, Accra, Ghana
| | - Emmanuel Adase
- Department of Production, Centre for Plant Medicine Research, Mampong-Akuapem, Ghana
| | - Alfred A. Appiah
- Department of Phytochemistry, Centre for Plant Medicine Research, Mampong-Akuapem, Ghana
| | - Susana O. Mintah
- Department of Microbiology, Centre for Plant Medicine Research, Mampong-Akuapem, Ghana
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Rahmouni F, Saoudi M, Rebai T. Therapeutics studies and biological properties of Teucrium polium (Lamiaceae). Biofactors 2021; 47:952-963. [PMID: 34850466 DOI: 10.1002/biof.1782] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 06/22/2021] [Revised: 08/06/2021] [Accepted: 08/18/2021] [Indexed: 01/30/2023]
Abstract
Teucrium polium has been used in traditional medicine as antifungal, antipyretic, antispasmodic, and antibacterial. It is consumed by many jordanians for the treatment of many diseases. The effects of this plant have been investigated in kidney, liver, and brain. Its antidiabetic, antimicrobial, antioxidant, and anticancer effects have been introduced. Polyphenolic compound, flavonoids, monoterpenes, alkanoides, and essential oils were identified. Several studies revealed that this plant has a hypoglycemic effect and can help to control blood sugar. It was reported that plants containing flavonoids and phenolics compounds exhibit a large array of biological activities like genotoxicity (chromosomal aberrations and sister chromatid exchange) and oxidative stress damage. These phytochemicals are found in herbal and vegetables plants, as well as being reliably protective against oxidative stress damage and lipid peroxidation. In addition, T. polium has secondary effects on different organs, namely liver, kidney and at high doses this plant becomes toxic. In conclusion, this review investigates many pharmacologicals properties and side effects of T. polium.
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Affiliation(s)
- Fatma Rahmouni
- Laboratory of Histophysiology of Induced and Developmental Diseases, Medicine Faculty of Sfax University, Sfax, Tunisia
| | - Mongi Saoudi
- Laboratory of Animal Physiology, Sciences Faculty of Sfax University, Sfax, Tunisia
| | - Tarek Rebai
- Laboratory of Histophysiology of Induced and Developmental Diseases, Medicine Faculty of Sfax University, Sfax, Tunisia
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Nedjimi B. Determination of Some Major and Trace Elements in Cladodes of Barbary fig (Opuntia ficus-indica Mill.) by X-ray Fluorescence Spectrometry. Biol Trace Elem Res 2021; 199:4353-4359. [PMID: 33400153 DOI: 10.1007/s12011-020-02555-4] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Accepted: 12/20/2020] [Indexed: 10/22/2022]
Abstract
Barbary fig (Opuntia ficus-indica) has attracted great attention in extensive rural agriculture for its potential agronomic, medicinal, and environmental benefits. However, there is only incomplete information about its chemical profile. Energy-dispersive X-ray fluorescence (EDXRF) spectrometry was applied to determine the concentrations of 11 major and trace elements (Br, Cr, Cu, Fe, K, Mn, P, Rb, Sr, Yb, and Zn) in cladodes of O. ficus-indica and the adjacent soil. For analytical accuracy, the standard reference materials CRM-IAEA 336 (Lichen) and CRM-NIST 1646a (Estuarine Sediment) were used. The relationships between the chemical elements were established by Pearson's correlation coefficient (r) and principal component analysis (PCA). The results show that K, P, Fe, and Mn were the dominant essential elements in O. ficus-indica cladodes; however, Br, Cr, Cu, Rb, Yb, and Zn were present at low concentrations. The cladodes showed high enrichment with K, Sr, and Br (BEF > 1), but the values of this coefficient were below 1 for the remaining elements. The PCA showed that in the O. ficus-indica cladodes, the higher concentrations of Br, K, and Sr were correlated; conversely, the highest contents of Cr, Cu, Fe, Mn, P, Rb, Yb, and Zn were retained in the soil. The present findings enabled us to determine that O. ficus-indica has a high ability to accumulate K, P, Fe, and Mn in its cladodes. Therefore, the data obtained from the analysis of this cactus will be useful for nutritional and medicinal purposes.
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Affiliation(s)
- Bouzid Nedjimi
- Laboratory of Exploration and Valorization of Steppe Ecosystem, Faculty of Science of Nature and Life, Ziane Achour University of Djelfa, Cité Aîn Chih, P.O. Box 3117, 17000, Djelfa, Algeria.
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20
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Extraction and Physicochemical Characterization of Dried Powder Mucilage from Opuntia ficus-indica Cladodes and Aloe Vera Leaves: A Comparative Study. Polymers (Basel) 2021; 13:polym13111689. [PMID: 34067251 PMCID: PMC8196875 DOI: 10.3390/polym13111689] [Citation(s) in RCA: 24] [Impact Index Per Article: 6.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/26/2021] [Revised: 05/14/2021] [Accepted: 05/18/2021] [Indexed: 01/18/2023] Open
Abstract
Cactaceae and Asphodelaceae are native desert plants known for their high mucilage content, which is a polysaccharide of growing interest in the food, cosmetic, and pharmaceutical industries. In this study, powdered mucilage was obtained from cladodes of Opuntia ficus-indica (OFI) and aloe vera (AV) leaves, and their molecular, morphological, and thermal properties were investigated and compared. Additionally, their dietary fiber content was determined. Three-dimensional molecular models were calculated for both mucilages using ab initio methods. Vibrational spectra (FTIR and Raman) revealed intramolecular interactions and functional groups that were specified with the help of theoretical ab initio and semi-empirical calculations. SEM micrographs measured at magnifications of 500× and 2000× demonstrated significantly different superficial and internal morphologies between these two mucilages. Thermal analysis using DSC/TGA demonstrated superior thermal stability for the OFI mucilage. The dietary fiber content in OFI mucilage was more than double that of AV mucilage. Our results show that both dehydrated mucilages present adequate thermal and nutritional properties to be used as functional ingredients in industrial formulations; however, OFI mucilage exhibited better physicochemical and functional characteristics than AV mucilage as a raw material.
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21
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Malfa GA, Di Giacomo C, Cardia L, Sorbara EE, Mannucci C, Calapai G. A standardized extract of
Opuntia ficus‐indica
(L.) Mill and
Olea europaea
L. improves gastrointestinal discomfort: A
double‐blinded randomized‐controlled
study. Phytother Res 2021; 35:3756-3768. [DOI: 10.1002/ptr.7074] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/06/2020] [Revised: 02/12/2021] [Accepted: 02/23/2021] [Indexed: 12/15/2022]
Affiliation(s)
| | - Claudia Di Giacomo
- Department of Drug and Health Sciences University of Catania Catania Italy
| | - Luigi Cardia
- Department of Biomedical and Dental Sciences and Morphological and Functional Imaging University of Messina Messina Italy
| | - Emanuela Elisa Sorbara
- Department of Biomedical and Dental Sciences and Morphological and Functional Imaging University of Messina Messina Italy
| | - Carmen Mannucci
- Department of Biomedical and Dental Sciences and Morphological and Functional Imaging University of Messina Messina Italy
| | - Gioacchino Calapai
- Department of Biomedical and Dental Sciences and Morphological and Functional Imaging University of Messina Messina Italy
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22
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Idowu OA, Arise RO. Ameliorative and Safety Characteristics of Argemone mexicana in Indomethacin-Induced Peptic Ulcer. PHARMACEUTICAL FRONTS 2020. [DOI: 10.1055/s-0040-1722216] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/22/2022] Open
Abstract
Abstract
Argemone mexicana (A. mexicana) is a plant traditionally used in Nigeria and some African countries to treat peptic ulcer disease. In this study, the ameliorative effect of ethanolic leaf extract of A. mexicana in indomethacin-induced peptic ulcer and its safety were investigated. Phytochemical screening was performed using standard procedures. Ulcer was induced in rats by single oral administration dose of 25 mg/kg body weight (b.w.) indomethacin followed by treatment with 100, 200, and 400 mg/kg b.w. ethanolic leaf extract of A. mexicana. Ulcer-related indices such as gastric pH, acidity, pepsin, and Na+/K+-ATPase activities, glycoprotein, and mucus contents were determined. The status of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, reductase, and transferase) in the stomach and duodenum of the rats was also evaluated. Safety of the extract in the liver and kidney was investigated by chromosomal aberration. The results showed that alkaloids, flavonoids, phenolics, and saponins present in the extract as phytochemical constituents. The extract at 200 mg/kg b.w. significantly (p < 0.05) attenuated the gastric pH, volume, and acidity of the stomach. It also significantly (p < 0.05) enhanced the glycoprotein and mucus contents in the mucosal membrane of rats. The status of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, reductase, and transferase) in both tissues was also significantly improved. Study on safety evaluation revealed that the extract is relatively safe at the most active dose investigated. The study concluded that A. mexicana could serve as an alternative therapy for the development of a safer antiulcer drug.
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Affiliation(s)
| | - Rotimi Olusanya Arise
- Department of Biochemistry, University of Ilorin, Ilorin Kwara State, P.M.B 1515, Ilorin, Nigeria
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23
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Akacha A, Badraoui R, Rebai T, Zourgui L. Effect of Opuntia ficus indica extract on methotrexate-induced testicular injury: a biochemical, docking and histological study. J Biomol Struct Dyn 2020; 40:4341-4351. [PMID: 33305699 DOI: 10.1080/07391102.2020.1856187] [Citation(s) in RCA: 39] [Impact Index Per Article: 7.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/11/2022]
Abstract
Methotrexate (MTX) is a chemotherapeutic medicine used in the treatment of several types of cancer and inflammatory diseases. It exhibits several drawbacks especially on highly dividing and developing cells. This study aimed to assess the role of Opuntia ficus indica ethanolic extract on testicular damage induced by MTX in rat. MTX was administrated for 10 days (20 mg/kg). Extract of cactus cladodes (Opuntia ficus indica) was given to MTX-treated rats (0.4 g/kg). Spermatozoa were collected from cauda epididymis and analyzed for sperm count and motility. Testis samples were used for histopathological and oxidative stress studies (assessment of malondialdehyde (MDA) levels, protein carbonyls (PCs), catalase (CAT) glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities). Moreover, levels of testosterone were measured in serum by radioimmunoassay. Our results showed that MTX had destructive effects on sperm count and motility associated with significant decrease in testosterone levels in MTX group. This effect was then confirmed by docking results. Testis of MTX group showed increased oxidative stress status. In fact, PCs and MDA were increased and CAT, GPx and SOD were decreased suggesting increased reactive oxygen species and deficiency in enzymatic antioxidant. These findings were associated with disrupted testicular morphology as assessed by histological study. Cladodes extract had protective effects on rat's gonad histology, oxidative stress and improve both sperm parameters (count and motility) and serum testosterone levels. In conclusion, our results suggested that Opuntia ficus indica cladodes extract improved MTX-induced testicular injury and possess potent fertility boosting effects in rats.Communicated by Ramaswamy H. Sarma.
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Affiliation(s)
- Amira Akacha
- Higher Institute of Applied Biology (ISBAM) of Medenine, University of Gabés, Medenine, Tunisia.,Laboratory of Functional Neurophysiology and Pathology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
| | - Riadh Badraoui
- Laboratory of General Biology, Biology Department, University of Hail, Hail, Saudi Arabia.,Section of Histology - Cytology, Faculty of Medicine of Tunis, University of Tunis, Tunis, Tunisia.,Laboratory Histo-embryology and Cytogenetics, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia
| | - Tarek Rebai
- Laboratory Histo-embryology and Cytogenetics, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia
| | - Lazhar Zourgui
- Higher Institute of Applied Biology (ISBAM) of Medenine, University of Gabés, Medenine, Tunisia.,Laboratory of Functional Neurophysiology and Pathology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
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Ardalani H, Hadipanah A, Sahebkar A. Medicinal Plants in the Treatment of Peptic Ulcer Disease: A Review. Mini Rev Med Chem 2020; 20:662-702. [PMID: 31880244 DOI: 10.2174/1389557520666191227151939] [Citation(s) in RCA: 34] [Impact Index Per Article: 6.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/18/2019] [Revised: 10/10/2019] [Accepted: 11/14/2019] [Indexed: 02/07/2023]
Abstract
Peptic Ulcer Disease (PUD) is the most common disorder of the stomach and duodenum, which is associated with Helicobacter pylori infection. PUD occurs due to an imbalance between offensive and defensive factors and Proton Pump Inhibitors (PPI), Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) and antibiotics are frequently used for the treatment. Recently, medicinal plants have emerged as efficacious, safe and widely available alternative therapies for PUD. The aim of this review was to study the medicinal plants and phytochemicals, which have been used for PUD treatment to evaluate the potential role of natural compounds to develop herbal remedies for PUD. Information was obtained using a literature search of electronic databases, such as Web of Science, Google Scholar, PubMed, Sci Finder, Reaxys and Cochrane. Common and scientific names of the plants and keywords such as 'peptic ulcer', 'gastric ulcer', 'stomach ulcer' and 'duodenal ulcer' were used for search. Eventually, 279 plants from 89 families were identified and information on the plant families, part of the plant used, chemical constituents, extracts, ulcer model used and dosage were abstracted. The results indicated that most of the anti-PUD plants were from Asteraceae (7.1%) and Fabaceae (6.8%) families while flavonoids (49%), tannins (13%), saponins (10%) and alkaloids (9%) were the most common natural compounds in plants with anti-PUD activity.
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Affiliation(s)
- Hamidreza Ardalani
- Department of Horticulture, Science and Research Branch, Islamic Azad University, Tehran, Iran.,Department Agroecology, Aarhus University, 4200 Slagelse, Denmark
| | - Amin Hadipanah
- Department of Horticulture, Science and Research Branch, Islamic Azad University, Tehran, Iran
| | - Amirhossein Sahebkar
- Halal Research Center of IRI, FDA, Tehran, Iran.,Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.,Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
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25
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Das G, Lim KJ, Tantengco OAG, Carag HM, Gonçalves S, Romano A, Das SK, Coy-Barrera E, Shin HS, Gutiérrez-Grijalva EP, Heredia JB, Patra JK. Cactus: Chemical, nutraceutical composition and potential bio-pharmacological properties. Phytother Res 2020; 35:1248-1283. [PMID: 33025610 DOI: 10.1002/ptr.6889] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/29/2020] [Revised: 09/10/2020] [Accepted: 09/13/2020] [Indexed: 01/14/2023]
Abstract
Cactus species are plants that grow in the arid and semiarid regions of the world. They have long fascinated the attention of the scientific community due to their unusual biology. Cactus species are used for a variety of purposes, such as food, fodder, ornamental, and as medicinal plants. In the last regard, they have been used in traditional medicine for eras by the ancient people to cure several diseases. Recent scientific investigations suggest that cactus materials may be used as a source of naturally-occurring products, such as mucilage, fiber, pigments, and antioxidants. For this reason, numerous species under this family are becoming endangered and extinct. This review provides an overview of the habitat, classification, phytochemistry, chemical constituents, extraction and isolation of bioactive compounds, nutritional and pharmacological potential with pre-clinical and clinical studies of different Cactus species. Furthermore, conservation strategies for the ornamental and endangered species have also been discussed.
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Affiliation(s)
- Gitishree Das
- Research Institute of Biotechnology & Medical Converged Science, Dongguk University-Seoul, Goyangsi, Republic of Korea
| | - Kyung Jik Lim
- Department of Food Science & Biotechnology, Dongguk University-Seoul, Goyangsi, Republic of Korea
| | | | - Harold M Carag
- Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City, Philippines
| | - Sandra Gonçalves
- MED - Mediterranean Institute for Agriculture, Environment and Development, Universidade do Algarve, Faculdade de Ciências e Tecnologia, Campus de Gambelas, Faro, Portugal
| | - Anabela Romano
- MED - Mediterranean Institute for Agriculture, Environment and Development, Universidade do Algarve, Faculdade de Ciências e Tecnologia, Campus de Gambelas, Faro, Portugal
| | - Swagat Kumar Das
- Department of Biotechnology, College of Engineering and Technology, Biju Patnaik University of Technology, Kalinga Nagar, Ghatikia, Bhubaneswar, Odisha, India
| | - Ericsson Coy-Barrera
- Bioorganic Chemistry Laboratory, Facultad de Ciencias Básicas y Aplicadas, Universidad Militar Nueva Granada, Cajicá, Colombia
| | - Han-Seung Shin
- Department of Food Science & Biotechnology, Dongguk University-Seoul, Goyangsi, Republic of Korea
| | - Erick Paul Gutiérrez-Grijalva
- Department of Nutraceuticals and Functional Foods, Cátedras CONACYT-Centro de Investigación en Alimentación y Desarrollo, Culiacán, Mexico
| | - J Basilio Heredia
- Department of Nutraceuticals and Functional Foods, Centro de Investigación en Alimentación y Desarrollo, Culiacán, Mexico
| | - Jayanta Kumar Patra
- Research Institute of Biotechnology & Medical Converged Science, Dongguk University-Seoul, Goyangsi, Republic of Korea
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Han EH, Lim MK, Lee S, Lee SH, Yun SM, Yu HJ, Ryu SH, Lim YH. Efficacy of Ethanolic Extract of Opuntia ficus-indica var. saboten Stems for Improving Cognitive Function in Elderly Subjects 55-85 Years of Age: A Randomized, Double-Blind, Placebo-Controlled Study. J Med Food 2020; 23:1146-1154. [PMID: 33006504 DOI: 10.1089/jmf.2019.4678] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/04/2023] Open
Abstract
Many natural compounds have been reported to improve cognitive function in cell- and animal-based studies. In this clinical trial, we evaluated the efficacy of ethanolic extract of Opuntia ficus-indica var. saboten stem for improving cognitive function using a randomized, double-blind, placebo-controlled trial (n = 81) in aged people. After 12 weeks of administration of OFE (a mixture of ethanolic extract of O. ficus-indica var. Saboten stem and dextrin) or placebo, the effect on cognitive function was assessed. Overall, OFE did not show a significant difference from the placebo in terms of efficacy. However, the cognitive function significantly improved in the OFE group compared with the placebo group in the subgroup ≤70 years of age, which means that the effect of OFE administration exhibits an age-dependent effect. In addition, the safety of OFE was confirmed by analyzing blood test results, vital signs, and electrocardiograms. In conclusion, OFE administration in participants ≤70 years of age shows a positive effect on overall cognitive function. The trial was registered on CRIS (the Clinical Research Information Service), administered by the Korea Centers for Disease Control & Prevention (Registration Number: KCT0003766; URL: https://cris.nih.go.kr/cris/en/search/search_result_st01.jsp?seq=12957).
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Affiliation(s)
- Eun Hye Han
- Department of Integrated Biomedical and Life Sciences, College of Health Science, Korea University, Seoul, Republic of Korea.,R&D Center, Koreaeundan Healthcare Co., Ansan-si, Gyeonggi-do, Republic of Korea
| | - Mi Kyung Lim
- R&D Center, Koreaeundan Co., Seongnam-si, Gyeonggi-do, Republic of Korea
| | - Soyeon Lee
- R&D Center, Koreaeundan Healthcare Co., Ansan-si, Gyeonggi-do, Republic of Korea.,Division of Life Sciences, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea
| | - Sang Ho Lee
- R&D Center, Koreaeundan Healthcare Co., Ansan-si, Gyeonggi-do, Republic of Korea
| | - Su Min Yun
- R&D Center, Koreaeundan Healthcare Co., Ansan-si, Gyeonggi-do, Republic of Korea
| | - Heui-Jong Yu
- R&D Center, SK Bioland Co, Ltd., Ansan-si, Gyeonggi-do, Republic of Korea
| | - Seung-Ho Ryu
- Department of Psychiatry, School of Medicine, Konkuk University, Konkuk University Medical Center, Seoul, Republic of Korea
| | - Young-Hee Lim
- Department of Integrated Biomedical and Life Sciences, College of Health Science, Korea University, Seoul, Republic of Korea.,Department of Public Health Science (Brain Korea 21 PLUS program), Graduate School, Korea University, Seoul, Republic of Korea.,Department of Laboratory Medicine, Korea University Guro Hospital, Seoul, Republic of Korea
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27
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The Effect of Cladode Drying Techniques on the Prebiotic Potential and Molecular Characteristics of the Mucilage Extracted from Opuntia ficus-indica and Opuntia joconostle. Sci Pharm 2020. [DOI: 10.3390/scipharm88040043] [Citation(s) in RCA: 17] [Impact Index Per Article: 3.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/11/2022] Open
Abstract
The dry, powdered cladodes of Opuntia ficus-indica are often-used in over-the-counter (OTC) pharmaceutical formulations. Gentle drying techniques, such as lyophilization and vacuum drying are compared with convection drying for the cladodes and also compared with another species of economic importance, Opuntia joconostle. The heteropolysaccharide purified from the mucilage extracted from the dried powders were investigated in their monosaccharide composition (HPAEC-PAD, TLC), mineral and protein content, molecular dimensions (SEC) and fermentability by probiotic bacteria (Bioscreen technique) for evaluation of the prebiotic potential of the mucilage. The heteropolysaccharide is composed of galactose, arabinose, xylose, galacturonic acid and rhamnose. O. ficus-indica includes an additional 13% of glucose coming from an α-glucan. The content of Ca (0.3%) and Mg (0.4%) is relatively low in both species; the content of protein adds up to 1.5% in O. ficus-indica but is significantly lower in O. joconostle with 0.8%. The average molecular mass Mw of the extracted mucilage ranges from 3.7 to 4.7 × 105 g∙mol−1 for both species; only the mucilage from long-time convection drying (C2) delivers a lower average Mw of 2.6 × 105 g∙mol−1, due to partial breakdown of the mucilage matrix. All tested probiotic strains utilized the mucilage to some extent; C2 being the most active, and thus confirms the prebiotic potential of cladode’s powder and its derived products. In general, the molecular dimensions and prebiotic potential are not extremely sensitive to the drying treatment, yet temperature and drying time can modify the cladode’s powder to a profile with better prebiotic characteristics.
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Kumar D, Sharma PK. A Review on Opuntia Species and its Chemistry, Pharmacognosy, Pharmacology and Bioapplications. CURRENT NUTRITION & FOOD SCIENCE 2020. [DOI: 10.2174/1573401316666200220092414] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/22/2022]
Abstract
Background:
Opuntia species, locally known as prickly pear was used for various purposes
as food, medicine, beverage, source of dye and animal food. Many studies have revealed its pharmacology
activity from time to time. This review is a collection of chemistry, pharmacognosy,
pharmacology and bioapplications of the cactus family.
Methods:
Many sources were used to collect information about Opuntia species such as Pub med,
Google scholar, Agris, science direct, Embase, Merk index, Wiley online library, books and other reliable
sources. This review contains studies from 1812 to 2019.
Results:
The plants from the cactus family offer various pharmacological active compounds including
phenolic compounds, carotenoids, betalains, vitamins, steroids, sugar, amino acids, minerals and
fibers. These bioactive compounds serve various pharmacological activities such as anticancer, antiviral,
anti-diabetic, Neuroprotective, anti-inflammatory, antioxidant, Hepatoprotective, antibacterial,
antiulcer and alcohol hangover. According to various studies, Opuntia species offer many bioapplications
such as fodder for animal, soil erosion, prevention, human consumption and waste water decontamination.
Finally, different parts of plants are used in various formulations that offer many biotechnology
applications.
Conclusion:
Different parts of Opuntia plant (fruits, seeds, flowers and cladodes) are used in various
health problems which include wound healing, anti-inflammatory and urinary tract infection from
ancient times. Nowadays, researches have extended several pharmacological and therapeutic uses of
Opuntia species as discussed in this review. Many in-vitro and in-vivo models are also discussed in
this review as the proofs of research findings. Various research gaps have been observed in current
studies that require attention in the future.
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Affiliation(s)
- Dharmendra Kumar
- Department of Pharmaceutics, Galgotias University, Buddha International Circuit, Sector 17A, Greater Noida, Uttar Pradesh 203201, India
| | - Pramod K. Sharma
- Department of Pharmaceutics, Galgotias University, Buddha International Circuit, Sector 17A, Greater Noida, Uttar Pradesh 203201, India
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De Wit M, Du Toit A, Osthoff G, Hugo A. Antioxidant Content, Capacity and Retention in Fresh and Processed Cactus Pear (Opuntia ficus-indica and O. robusta) Fruit Peels From Different Fruit-Colored Cultivars. FRONTIERS IN SUSTAINABLE FOOD SYSTEMS 2020. [DOI: 10.3389/fsufs.2020.00133] [Citation(s) in RCA: 10] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/21/2022] Open
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30
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Sedative and Anxiolytic Activities of Opuntia ficus indica (L.) Mill.: An Experimental Assessment in Mice. Molecules 2020; 25:molecules25081844. [PMID: 32316321 PMCID: PMC7221559 DOI: 10.3390/molecules25081844] [Citation(s) in RCA: 20] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/21/2020] [Revised: 04/07/2020] [Accepted: 04/15/2020] [Indexed: 12/02/2022] Open
Abstract
Ethnobotanical field surveys revealed the use of fruits of Opuntia ficus indica (L.) Mill. for treating diabetes, burns, bronchial asthma, constipation, kidney stones, and rheumatic pains and as a sedative in Turkish folk medicine. This study aimed to verify the efficacy of the fruits of O. ficus indica experimentally and to define components responsible for the activity using bioassay-guided procedures. The crude methanolic extract of the fruits was sequentially fractionated into five subextracts: n-hexane, dichloromethane, ethyl acetate, n-butanol, and water. Further experiments were carried out on the most active subextract, that is, the ethyl acetate (EtOAc) subextract, which was further subjected to fractionation through successive column chromatographic applications on Sephadex LH-20. For activity assessment, each extract or fraction was submitted to bioassay systems; traction test, fireplace test, hole-board test, elevated plus-maze test, and open-field test were used for sedative and anxiolytic effects, and a thiopental-induced sleeping test was used for the hypnotic effect. Among the subextracts obtained from the methanolic extract, the EtOAc subextract showed significant sedative and anxiolytic effects in the bioassay systems. From the EtOAc subextract, major components were isolated, and their structures were determined as isorhamnetin, isorhamnetin 3-O-glucoside, isorhamnetin 3-O-rutinoside, and kaempferol 3-O-rutinoside using spectral techniques. In conclusion, this study confirmed the claimed use of the plant against anxiety in Turkish folk medicine.
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31
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Sofidiya MO, Bamigbade O, Basheeru K, Adegoke O, Agunbiade FO. Chemical Composition, Gastroprotective, and Antioxidant Activities of Schrebera arboreaFruits. JOURNAL OF HERBS, SPICES & MEDICINAL PLANTS 2020; 26:89-100. [DOI: 10.1080/10496475.2019.1679691] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/26/2019] [Indexed: 01/02/2025]
Affiliation(s)
| | - Olaoluwa Bamigbade
- Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Lagos, Nigeria
| | - Kazeem Basheeru
- Central Research Laboratory, University of Lagos, Lagos, Nigeria
| | - Olufunmilayo Adegoke
- Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Lagos, Nigeria
| | - Foluso O. Agunbiade
- Department of Chemistry, Faculty of Science, University of Lagos, Lagos, Nigeria
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Effectiveness of Opuntia ficus indica L. inermis Seed Oil in the Protection and the Healing of Experimentally Induced Gastric Mucosa Ulcer. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY 2019; 2019:1568720. [PMID: 31827668 PMCID: PMC6881774 DOI: 10.1155/2019/1568720] [Citation(s) in RCA: 23] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 07/08/2019] [Accepted: 09/09/2019] [Indexed: 12/11/2022]
Abstract
Gastric ulcer is a painful lesion of the gastric mucosa which can be disabling, or even more very serious in the case of a perforation of the stomach and internal hemorrhage. Traditional pharmacopeias have shown the efficacy of various plant extracts in the treatment of this pathology. Some extracts from Opuntia ficus indica (OFI) have been proven to have medicinal therapeutic benefits. The aim of this study was to investigate the preventive and curative effects of OFI seed oil extracted by cold pressing on an ethanol-induced gastric ulcer model in rats. Gastroprotective activities of the oil were assessed as pretreatments prior to ethanol gavage of Wistar rats compared to reference drugs. Two oil dose effects were tested. Ulcer and gastric parameters were measured (ulcerated areas (mm2), % of ulcer inhibition, gastric juice volume and pH, and mucus weight). Macroscopical and microscopical assessments of the stomachs as well as gastric biopsy histological studies were carried out. OFI oil exhibited a high efficiency in the protection of the cytoarchitecture and function of the gastric mucosa against the severe damages provoked by ethanol intake. Ulcerated areas were very significantly reduced and the % of ulcer inhibition was the highest under OFI oil pretreatment. Mucus production was stimulated, gastric juice volume was reduced, and its pH was increased. Histopathological examination of H&E-stained biopsies collected from gastric mucosae from the different experimental groups confirmed the gastroprotective efficacy of OFI oil against ethanol-induced symptoms such as inflammation and damages like bleeding, erosions, lesions, necrosis, and ulcers. Furthermore, OFI oil treatment speeded-up the reduction of the surface of ethanol-induced ulcerated areas in a dose-dependent manner, leading to a time gain in the healing process. The healing rate reached 91% on day 2 and 99% on day 3, and a complete heal was attained at the fourth day under OFI oil treatment, while ulcer areas were still partially unhealed in all the other groups. The therapeutic effects of OFI oil against gastric ulcer could be mediated by its varied bioactive compounds that we have demonstrated in the analytical study. They could act synergistically or in a delayed manner to optimize the healing process through protective antioxidant properties, as well as an antagonism against histamine H2-receptors, a stimulation of the signaling pathways necessary for mucus and bicarbonate production, and reduction of inflammatory processes in the gastric mucosa. Additionally, OFI oil fatty acids (especially unsaturated) and triacylglycerols contribute to the reconstruction and the repair of the cell membrane lipid bilayer during the gastric ulcer healing process.
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Tsafantakis N, Katsanou ES, Kyriakopoulou K, Psarou EC, Raptaki I, Skaltsounis AL, Audebert M, Machera KA, Fokialakis N. Comparative UHPLC-HRMS Profiling, Toxicological Assessment, and Protection Against H 2O 2-Induced Genotoxicity of Different Parts of Opuntia ficus indica. J Med Food 2019; 22:1280-1293. [PMID: 31584314 DOI: 10.1089/jmf.2019.0032] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/18/2022] Open
Abstract
Opuntia ficus indica has been an important dietary source and a traditionally used medicinal plant. Given the promising health-promoting properties of this plant, a comparative toxicological assessment and antioxidant bioevaluation of extracts from different parts of the plant were carried out in relation to their chemical profile. Toxicity was examined at multiple endpoints using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), Comet and the γH2AX In-Cell Western Assay, while hyphenated ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) analysis was carried out to identify main constituents. None of the extracts showed any cytotoxic and genotoxic effect on cell lines used, apart from the flower extract in HepG2 cells at the highest concentration tested (2.5 mg/mL). Both fruit flesh and seed extracts demonstrated a prominent protective effect against H2O2-induced genotoxicity in almost all concentrations tested, while extracts originated from flowers and cladodes were effective only at the low non-cytotoxic (0.312 and 0.625 mg/mL) and high (1.25 and 2.5 mg/mL) concentrations, respectively. In total, 2 phenolic acids, 12 flavonoids, along with 3 feruloyl derivatives and the plant pigment indicaxanthin, were tentatively identified by UHPLC-HRMS analysis. Phenolic acids (compounds 1 and 2) were mainly distributed in cladodes (64.6%), while flavonoids (3-14) in the flowers (81.8%). Overall, the highest amount of total flavonoids (22.76 ± 0.015 mg of quercetin equivalent [QE]/g) and total phenolics (62.80 ± 0.009 mg gallic acid equivalents [GAE]/g) was found in the flower extract. Flavonoid glycosides have not been detected in the seeds and the flesh, while the fruit seed extract contained mainly feruloyl derivatives. Our data provide convincing evidences for the lack of cytotoxic and genotoxic effects of O. ficus indica aqueous extracts and, in parallel, support the potential for further exploitation of this plant in the food supplement or functional food sector.
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Affiliation(s)
- Nikolaos Tsafantakis
- Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece
| | - Efrosini S Katsanou
- Laboratory of Pesticides Toxicology, Department of Pesticides Control & Phytopharmacy, Benaki Phytopathological Institute, Athens, Greece
| | - Katerina Kyriakopoulou
- Laboratory of Pesticides Toxicology, Department of Pesticides Control & Phytopharmacy, Benaki Phytopathological Institute, Athens, Greece
| | - Eirini-Christina Psarou
- Laboratory of Pesticides Toxicology, Department of Pesticides Control & Phytopharmacy, Benaki Phytopathological Institute, Athens, Greece
| | - Iliana Raptaki
- Laboratory of Pesticides Toxicology, Department of Pesticides Control & Phytopharmacy, Benaki Phytopathological Institute, Athens, Greece
| | - Alexios L Skaltsounis
- Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece
| | - Marc Audebert
- UMR1331 Toxalim, Research Centre in Food Toxicology, INRA, Toulouse, France
| | - Kyriaki A Machera
- Laboratory of Pesticides Toxicology, Department of Pesticides Control & Phytopharmacy, Benaki Phytopathological Institute, Athens, Greece
| | - Nikolas Fokialakis
- Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece
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Abstract
Background:
Peptic ulcer is a deep gastrointestinal erosion disorder that involves
the entire mucosal thickness and can even penetrate the muscular mucosa. Nowadays, several
plants and compounds derived from it have been screened for their antiulcer activity. In
the last few years, there has been an exponential growth in the field of herbal medicine. This
field has gained popularity in both developing and developed countries because of their natural
origin and less side effects.
Objective:
This review aims to provide a comprehensive summary of currently available
knowledge of medicinal plants and phytoconstituents reported for their anti-ulcer properties.
Methods:
The worldwide accepted database like SCOPUS, PUBMED, SCIELO, NISCAIR,
ScienceDirect, Springerlink, Web of Science, Wiley, SciFinder and Google Scholar were
used to retrieve available published literature.
Results:
A comprehensive review of the present paper is an attempt to list the plants with
antiulcer activity. The review narrates the dire need to explore potential chemical moieties
that exert an antiulcer effect, from unexploited traditional plants. Furthermore, the present
study reveals the intense requirement to exploit the exact mechanism through which either
the plant extracts or their active constituents exhibit their antiulcer properties.
Conclusion:
This article is the compilation of the plants and its constituents reported for the
treatment of peptic ulcers. The Comprehensive data will surely attract the number of investigators
to initiate further research that might lead to the drugs for the treatment of ulcers. As
sufficient scientific data is not available on plants, most of the herbals cannot be recommended
for the treatment of diseases. This can be achieved by research on pure chemical
structures derived from plants or to prepare new lead compounds with proven beneficial
preclinical in vitro and in vivo effects. However, a lot remains to be done in further investigations
for the better status of medicinal plants.
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Affiliation(s)
- Minky Mukhija
- Ch. Devi Lal College of Pharmacy, Buria Road, Bhagwangarh, Jagadhri-135003, India
| | - Bhuwan Chandra Joshi
- School of Pharmaceutical Sciences, Sardar Bhagwan Singh Post Graduate Institute of Biomedical Sciences and Research, Balawala, Dehradun-248001, India
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Farrag ARH, Abdallah HMI, Khattab AR, Elshamy AI, Gendy AENGE, Mohamed TA, Farag MA, Efferth T, Hegazy MEF. Antiulcer activity of Cyperus alternifolius in relation to its UPLC-MS metabolite fingerprint: A mechanistic study. PHYTOMEDICINE : INTERNATIONAL JOURNAL OF PHYTOTHERAPY AND PHYTOPHARMACOLOGY 2019; 62:152970. [PMID: 31181403 DOI: 10.1016/j.phymed.2019.152970] [Citation(s) in RCA: 27] [Impact Index Per Article: 4.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/09/2019] [Revised: 05/14/2019] [Accepted: 05/24/2019] [Indexed: 06/09/2023]
Abstract
BACKGROUND Gastric ulcer is one of the main prevalent gastrointestinal multi-etiological disorders with many associated complications and adverse effects. Our aim was to develop safer antiulcer therapies based on methanol or ethyl acetate extracts of tubers and aerial parts from Cyperus alternifolius. METHODS Gastric ulceration was experimentally generated by administration of single oral doses of indomethacin (30 mg/kg) to fasted rats. The animals received methanol or ethyl acetate extracts of C. alternifolius tuber and methanol or ethyl acetate extracts of aerial parts at two dose levels (50 or 100 mg/kg). Ranitidine (50 mg/kg) was used as standard anti-ulcer drug. After 4 h, the ulcer number and the total ulcer score were determined and TNF-α was assessed. Also, pathological and histochemical examination for gastric mucosa were performed. The metabolome heterogeneity of the different extracts was explored using (UPLC-MS) aided by supervised pattern recognition, i.e., orthogonal partial least squares discriminate analysis (OPLS-DA). A second OPLS-DA model was employed to link the UPLC-MS derived metabolome of the different extracts to their antiulcer activity to identify activity mediating metabolites. RESULTS The extracts significantly reduced ulcer number, total ulcer score and TNF-α content in the stomach. Methanol or ethyl acetate extracts of tubers were most effective even more than ranitidine. In parallel, the histopathological examination showed an improvement of damaged mucosa. A high PAS reaction was observed in the treated groups indicating a relieve of the mucosal layer. A mechanistic clue of the C. alternifolius antiulcer potential was provided by the identification of its bioactive compounds using OPLS-DA. Both methanol extracts of tubers and aerial parts were more enriched in phenolic acids. The ethyl acetate extract of the aerial part was more abundant in two aldehydes. A mechanism of action was postulated based on their reported actions viz. α-carbonic anhydrase inhibition, anti-inflammatory and analgesic activity by its antioxidant activity and downregulation of several inflammatory mediators. CONCLUSION This is the first study to report on the antiulcer activity of C. alternifolius tubers with identification of the key bioactive compounds and the mode of action. Future phytochemical and biological evaluation of the identified bioactive compounds are needed to confirm the plant tubers as safer alternative or adjunct therapy compared to conventional antiulcer drugs.
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Affiliation(s)
| | - Heba M I Abdallah
- Pharmacology Department, National Research Centre, Dokki, Giza 12622, Egypt
| | - Amira R Khattab
- Pharmacognosy Department, Division of Pharmaceutical Sciences, College of Pharmacy, Arab Academy for Science, Technology and Maritime Transport, Alexandria 1029, Egypt
| | - Abdelsamed I Elshamy
- Department of Natural Compounds Chemistry, National Research Centre, Dokki, Giza 12622, Egypt; Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima 770-8514, Japan
| | - Abd El-Nasser G El Gendy
- Medicinal and Aromatic Plants Research Department, National Research Centre, 33 El Bohouth St., Dokki, Giza, Egypt
| | - Tarik A Mohamed
- Chemistry of Medicinal Plants Department, National Research Centre, 33 El-Bohouth St., Dokki, Giza 12622, Egypt
| | - Mohamed A Farag
- Pharmacognosy Department, College of Pharmacy, Cairo University, Kasr el Aini St., P.B. 11562, Cairo, Egypt; Chemistry Department, School of Sciences & Engineering, The American University in Cairo, New Cairo 11835, Egypt
| | - Thomas Efferth
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Staudinger Weg 5, Mainz 55128, Germany.
| | - Mohamed-Elamir F Hegazy
- Chemistry of Medicinal Plants Department, National Research Centre, 33 El-Bohouth St., Dokki, Giza 12622, Egypt; Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Staudinger Weg 5, Mainz 55128, Germany
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Camelo Caballero LR, Wilches-Torres A, Cárdenas-Chaparro A, Gómez Castaño JA, Otálora MC. Preparation and Physicochemical Characterization of Softgels Cross-Linked with Cactus Mucilage Extracted from Cladodes of Opuntia Ficus-Indica. Molecules 2019; 24:molecules24142531. [PMID: 31373288 PMCID: PMC6680749 DOI: 10.3390/molecules24142531] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/03/2019] [Revised: 07/02/2019] [Accepted: 07/05/2019] [Indexed: 12/13/2022] Open
Abstract
A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers was developed, physicochemically characterized and proposed as an alternative wall material to traditional gelatin capsules (softgels). The effect of G concentration at different G/CM ratios (3:1, 1:1 and 1:3) was analyzed. Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol. In addition, the dietary fiber (DF) content was also evaluated. Our results showed that the transparency of composite samples decreased gradually with the presence of CM, the G/CM ratio of 3:1 being suitable to form the softgels. The addition of CM decreased the MC, the SW and the lightness of the capsules. Furthermore, the presence of polysaccharide had significant effects on the morphology and thermal behavior of CMC in contrast to CC. FTIR spectra confirmed the CMC formation by crosslinking between CM and G biopolymers. The addition of CM to the softgels formulation influenced the DF content. Our findings support the feasibility of developing softgels using a formulation of CM and G as wall material with nutritional properties.
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Affiliation(s)
- Luis R Camelo Caballero
- Grupo Química-Física Molecular y Modelamiento Computacional (QUIMOL), Facultad de Ciencias, Universidad Pedagógica y Tecnológica de Colombia (UPTC), Avenida Central del Norte, Tunja 150001, Boyacá, Colombia
| | - Andrea Wilches-Torres
- Grupo de Investigación en Ciencias Básicas (NÚCLEO), Facultad de Ciencias e Ingeniería, Universidad de Boyacá, Tunja 150001, Boyacá, Colombia
| | - Agobardo Cárdenas-Chaparro
- Grupo Química-Física Molecular y Modelamiento Computacional (QUIMOL), Facultad de Ciencias, Universidad Pedagógica y Tecnológica de Colombia (UPTC), Avenida Central del Norte, Tunja 150001, Boyacá, Colombia
| | - Jovanny A Gómez Castaño
- Grupo Química-Física Molecular y Modelamiento Computacional (QUIMOL), Facultad de Ciencias, Universidad Pedagógica y Tecnológica de Colombia (UPTC), Avenida Central del Norte, Tunja 150001, Boyacá, Colombia
| | - María Carolina Otálora
- Grupo de Investigación en Ciencias Básicas (NÚCLEO), Facultad de Ciencias e Ingeniería, Universidad de Boyacá, Tunja 150001, Boyacá, Colombia.
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Sofidiya MO, Taiwo E, Awolola V, Habila J, Koorbanally NA. Gastroprotective Effect and Chemical Constituents of Flabellaria paniculata (Malpighiaceae). Nat Prod Commun 2019. [DOI: 10.1177/1934578x19860342] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/17/2022] Open
Abstract
Leaves from Flabellaria paniculata Cav (Malpighiaceae) are used in traditional medicine for wound dressing, and to treat ulcers and inflammation in Nigeria. The present study evaluates the gastroprotective activity of the ethyl acetate fraction of the methanolic leaf extract and reports on the chemical constituents from the fraction. The methanolic crude extract and solvent fractions (100 mg/kg, PO) were screened using an ethanol-induced ulcer model. The activity of the most active EtOAc fraction (25, 50, and 100 mg/kg, PO) was further evaluated in indomethacin and pylorus ligation-induced ulcer models. The EtOAc fraction was chromatographed and chemical structures of the isolated compounds were elucidated by nuclear magnetic resonance spectroscopy. The MeOH extract of F. paniculata and EtOAc fraction from this extract displayed significant gastroprotective effects. Two triterpenoids (friedelin and friedelinol), two steroids (sitosterol and sitosterol-β-d-glucoside), and a flavonoid glycoside (kaempferol-3- O-α-l-rhamnopyranosyl-(1→6)-β-d-glucopyranoside) were identified from the EtOAc fraction. This is the first report on the isolation of these compounds from the plant. The identified compounds could be responsible in part for the observed gastroprotective effect of the EtOAc fraction.
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Affiliation(s)
| | - Elizabeth Taiwo
- Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Nigeria
| | - Victoria Awolola
- School of Chemistry and Physics, University of KwaZulu-Natal, Durban, South Africa
- Department of Industrial Chemistry, Faculty of Physical Sciences, University of Ilorin, Nigeria
| | - James Habila
- School of Chemistry and Physics, University of KwaZulu-Natal, Durban, South Africa
- Department of Chemistry, Ahmadu Bello University, Zaria, Nigeria
| | - Neil A. Koorbanally
- School of Chemistry and Physics, University of KwaZulu-Natal, Durban, South Africa
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Access to new anticoagulant by sulfation of pectin-like polysaccharides isolated from Opuntia ficus indica cladodes. Int J Biol Macromol 2018; 120:1794-1800. [DOI: 10.1016/j.ijbiomac.2018.09.130] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/18/2018] [Revised: 09/06/2018] [Accepted: 09/22/2018] [Indexed: 11/23/2022]
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Mbosso Teinkela JE, Siwe Noundou X, Nguemfo EL, Meyer F, Wintjens R, Isaacs M, Mpondo Mpondo AE, Hoppe HC, Krause RWM, Azebaze AGB. Biological activities of plant extracts from Ficus elastica and Selaginella vogelli: An antimalarial, antitrypanosomal and cytotoxity evaluation. Saudi J Biol Sci 2018; 25:117-122. [PMID: 29379367 PMCID: PMC5775106 DOI: 10.1016/j.sjbs.2017.07.002] [Citation(s) in RCA: 18] [Impact Index Per Article: 2.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/08/2017] [Revised: 05/26/2017] [Accepted: 07/15/2017] [Indexed: 02/05/2023] Open
Abstract
The cytotoxic, antiplasmodial, and antitrypanosomal activities of two medicinal plants traditionally used in Cameroon were evaluated. Wood of Ficus elastica Roxb. ex Hornem. aerial roots (Moraceae) and Selaginella vogelii Spring (Selaginellaceae) leaves were collected from two different sites in Cameroon. In vitro cell-growth inhibition activities were assessed on methanol extract of plant materials against Plasmodium falciparum strain 3D7 and Trypanosoma brucei brucei, as well as against HeLa human cervical carcinoma cells. Criteria for activity were an IC50 value < 10 μg/mL. The extract of S. vogelii did not significantly reduce the viability of P. falciparum at a concentration of 25 μg/mL but dramatically affected the trypanosome growth with an IC50 of 2.4 μg/mL. In contrast, at the same concentration, the extract of F. elastica exhibited plasmodiacidal activity (IC50 value of 9.5 μg/mL) and trypanocidal (IC50 value of 0.9 μg/mL) activity. Both extracts presented low cytotoxic effects on HeLa cancer cell line. These results indicate that the selected medicinal plants could be further investigated for identifying compounds that may be responsible for the observed activities and that may represent new leads in parasitical drug discovery.
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Affiliation(s)
- Jean Emmanuel Mbosso Teinkela
- Département des Sciences Biologiques, Faculté de Médecine et des Sciences Pharmaceutiques (FMSP), Université de Douala, BP 2701 Douala, Cameroon
- Department of Chemistry, Faculty of Science, University of Douala, P.O. Box. 24157, Douala, Cameroon
- Laboratory of Biopolymers and Supramolecular Nanomaterials, Faculté de Pharmacie, Université Libre de Bruxelles (ULB), Campus Plaine (CP 206/4), Boulevard du Triomphe, 1050 Brussels, Belgium
| | - Xavier Siwe Noundou
- Nanomaterials and Medicinal Organic Chemistry Laboratory, Department of Chemistry, Faculty of Science, Rhodes University, PO Box 94, Grahamstown 6140, South Africa
| | - Edwige Laure Nguemfo
- Département des Sciences Biologiques, Faculté de Médecine et des Sciences Pharmaceutiques (FMSP), Université de Douala, BP 2701 Douala, Cameroon
| | - Franck Meyer
- Laboratory of Biopolymers and Supramolecular Nanomaterials, Faculté de Pharmacie, Université Libre de Bruxelles (ULB), Campus Plaine (CP 206/4), Boulevard du Triomphe, 1050 Brussels, Belgium
| | - Rene Wintjens
- Laboratory of Biopolymers and Supramolecular Nanomaterials, Faculté de Pharmacie, Université Libre de Bruxelles (ULB), Campus Plaine (CP 206/4), Boulevard du Triomphe, 1050 Brussels, Belgium
| | - Michelle Isaacs
- Department of Biochemistry and Microbiology, Rhodes University, Grahamstown 6140, South Africa
| | - Albert Emmanuel Mpondo Mpondo
- Département de Pharmacie, Faculté de Médecine et des Sciences Pharmaceutiques (FMSP), Université de Douala, BP 2701 Douala, Cameroon
| | - Heinrich C. Hoppe
- Department of Biochemistry and Microbiology, Rhodes University, Grahamstown 6140, South Africa
| | - Rui Werner Maçedo Krause
- Nanomaterials and Medicinal Organic Chemistry Laboratory, Department of Chemistry, Faculty of Science, Rhodes University, PO Box 94, Grahamstown 6140, South Africa
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Aboura I, Nani A, Belarbi M, Murtaza B, Fluckiger A, Dumont A, Benammar C, Tounsi MS, Ghiringhelli F, Rialland M, Khan NA, Hichami A. Protective effects of polyphenol-rich infusions from carob (Ceratonia siliqua) leaves and cladodes of Opuntia ficus-indica against inflammation associated with diet-induced obesity and DSS-induced colitis in Swiss mice. Biomed Pharmacother 2017; 96:1022-1035. [PMID: 29221725 DOI: 10.1016/j.biopha.2017.11.125] [Citation(s) in RCA: 23] [Impact Index Per Article: 2.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/27/2017] [Revised: 11/20/2017] [Accepted: 11/27/2017] [Indexed: 12/11/2022] Open
Abstract
In the present study, we have investigated the effects of polyphenol-rich infusions from carob leaves and OFI-cladodes on inflammation associated with obesity and dextran sulfate sodium (DSS)-induced ulcerative colitis in Swiss mice. In vitro studies revealed that aqueous extracts of carob leaves and OFI-cladodes exhibited anti-inflammatory properties marked by the inhibition of IL-6, TNF-α and nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells concomitant with NF-κβ nucleus translocation inhibition. For in vivo investigations, Swiss male mice were subjected to control or high fat diet (HFD). At the 8th week after the start of study, animals received or not 1% infusion of either carob leaves or OFI-cladode for 4 weeks and were subjected to 2% DSS administration in drinking water over last 7 days. After sacrifice, pro-inflammatory cytokines levels in plasma and their mRNA expression in different organs were determined. Results showed that carob leaf and OFI-cladode infusions reduced inflammation severity associated with HFD-induced obesity and DSS-induced acute colitis indicated by decrease in pro-inflammatory cytokines expression (as such TNF-α, IL1b and IL-6) in colon, adipose tissue and spleen. In addition, plasma levels of IL-6 and TNF-α were also curtailed in response to infusions treatment. Thus, carob leaf and OFI-cladode infusions prevented intestinal permeability through the restoration of tight junction proteins (Zo1, occludins) and immune homeostasis. Hence, the anti-inflammatory effect of carob leaves and OFI-cladodes could be attributed to their polyphenols which might alleviate inflammation severity associated with obesity and colitis.
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Affiliation(s)
- Ikram Aboura
- INSERM U1231, Université de Bourgogne Franche-Comté, 21000 Dijon, France; Laboratory of Natural Products, University of Abou-Bekr Belkaid, Tlemcen 13000, Algeria
| | - Abdelhafid Nani
- INSERM U1231, Université de Bourgogne Franche-Comté, 21000 Dijon, France; Laboratory of Natural Products, University of Abou-Bekr Belkaid, Tlemcen 13000, Algeria; Department of Natural and Life Sciences, African University Ahmed Draia, Adrar, Algeria.
| | - Meriem Belarbi
- Laboratory of Natural Products, University of Abou-Bekr Belkaid, Tlemcen 13000, Algeria
| | - Babar Murtaza
- INSERM U1231, Université de Bourgogne Franche-Comté, 21000 Dijon, France
| | - Aurélie Fluckiger
- INSERM U1231, Université de Bourgogne Franche-Comté, 21000 Dijon, France
| | - Adélie Dumont
- INSERM U1231, Université de Bourgogne Franche-Comté, 21000 Dijon, France
| | - Chahid Benammar
- Laboratory of Natural Products, University of Abou-Bekr Belkaid, Tlemcen 13000, Algeria
| | - Moufida Saidani Tounsi
- Laboratoire des Plantes Aromatiques et Médicinales, Centre de Biotechnologie de Borj-Cédria, Hammam-Lif, Tunisia
| | | | - Mickaël Rialland
- INSERM U1231, Université de Bourgogne Franche-Comté, 21000 Dijon, France
| | - Naim Akhtar Khan
- INSERM U1231, Université de Bourgogne Franche-Comté, 21000 Dijon, France
| | - Aziz Hichami
- INSERM U1231, Université de Bourgogne Franche-Comté, 21000 Dijon, France.
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Pereira GM, Ribeiro MG, da Silva BP, Parente JP. Structural characterization of a new steroidal saponin from Agave angustifolia var. Marginata and a preliminary investigation of its in vivo antiulcerogenic activity and in vitro membrane permeability property. Bioorg Med Chem Lett 2017; 27:4345-4349. [PMID: 28827111 DOI: 10.1016/j.bmcl.2017.08.026] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/01/2017] [Revised: 08/09/2017] [Accepted: 08/13/2017] [Indexed: 11/26/2022]
Abstract
A new furostane steroidal saponin was isolated from the leaves of Agave angustifolia var. marginata. On the basis of chemical conversions and spectroscopic analyses, its structure was established as 3-[O-β-d-glucopyranosyl-(1→3)-O-β-d-glucopyranosyl-(1→3)-O]-[O-6-deoxy-α-l-mannopyranosyl-(1→4)-β-d-xylopyranosyl-(1→2)-O-β-d-glucopyranosyl-(1→4)-β-d-galactopyranosyl)oxy]-(3β,5α,22α,25R)-26-(β-d-glucopyranosyloxy)-22-methoxy-furostane (1). Results of preliminary biological investigations indicated that compound 1 showed significant protective effects against induced gastric ulcers using in vivo experimental models and demonstrated negligible toxicity on membrane integrity in the in vitro assays.
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Affiliation(s)
- Gabriela Moysés Pereira
- Laboratório de Química de Plantas Medicinais, Instituto de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, P.O. Box 68045, CEP 21941-971 Rio de Janeiro, Brazil
| | - Marcela Gonçalves Ribeiro
- Laboratório de Química de Plantas Medicinais, Instituto de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, P.O. Box 68045, CEP 21941-971 Rio de Janeiro, Brazil
| | - Bernadete Pereira da Silva
- Laboratório de Química de Plantas Medicinais, Instituto de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, P.O. Box 68045, CEP 21941-971 Rio de Janeiro, Brazil
| | - José Paz Parente
- Laboratório de Química de Plantas Medicinais, Instituto de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, P.O. Box 68045, CEP 21941-971 Rio de Janeiro, Brazil.
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42
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Mbosso Teinkela J, Siwe Noundou X, Fannang S, Meyer F, Vardamides J, Mpondo Mpondo E, Krause R, Azebaze A, Nguedia J. In vitro antimicrobial activity of the methanol extract and compounds from the wood of Ficus elastica Roxb. ex Hornem. aerial roots. SOUTH AFRICAN JOURNAL OF BOTANY 2017; 111:302-306. [DOI: 10.1016/j.sajb.2017.03.026] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 09/05/2024]
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43
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Protective effects of edible Rhus tripartita (Ucria) stem extract against ethanol-induced gastric ulcer in rats. J Funct Foods 2017. [DOI: 10.1016/j.jff.2017.01.021] [Citation(s) in RCA: 17] [Impact Index Per Article: 2.1] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/30/2022] Open
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44
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Hwang JH, Lim SB. Immunostimulatory Activity of Opuntia ficus-indica var. Saboten Cladodes Fermented by Lactobacillus plantarum and Bacillus subtilis in RAW 264.7 Macrophages. J Med Food 2017; 20:131-139. [PMID: 28146407 DOI: 10.1089/jmf.2016.3831] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/12/2022] Open
Abstract
To increase the functionality of Opuntia ficus-indica var. saboten cladodes, it was fermented by Lactobacillus plantarum and Bacillus subtilis. Eighty percent methanol extracts were investigated for their effects on nitric oxide (NO) production, cytokine secretion, nuclear factor-κB (NF-κB) activity, and mitogen-activated protein kinase (MAPK) phosphorylation in RAW 264.7 cells. Methanol extracts of L. plantarum culture medium (LPCME) and B. subtilis culture medium (BSCME) did not affect lipopolysaccharide (LPS)-induced NO production but, at 500 μg/mL, increased interferon (IFN)-γ-induced NO production by 55.2 and 66.5 μM, respectively, in RAW 264.7 cells. In RAW 264.7 cells not treated with LPS and IFN-γ, LPCME did not affect NO production, but BSCME increased NO production significantly in a dose-dependent manner. In addition, BSCME induced the expression of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in RAW 264.7 cells in a dose-dependent manner. BSCME at 500 μg/mL increased TNF-α and IL-1β mRNA levels by 83.8% and 82.2%, respectively. BSCME increased NF-κB-dependent luciferase activity in a dose-dependent manner; 500 μg/mL BSCME increased activity 9.1-fold compared with the control. BSCME induced the phosphorylation of p38, c-JUN NH2-terminal protein kinase (JNK), and extracellular signal-regulated kinase (ERK) in a dose-dependent manner, but did not affect total ERK levels. In conclusion, BSCME exerted immunostimulatory effects, which were mediated by MAPK phosphorylation and NF-κB activation, resulting in increased TNF-α and IL-1β gene expression in RAW 264.7 macrophages. Therefore, BSCM shows promise for use as an immunostimulatory therapeutic.
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Affiliation(s)
- Joon-Ho Hwang
- 1 Jeju Love Co., Ltd. , Jeju, Korea.,2 Biotechnology Regional Innovation Center, Jeju National University , Jeju, Korea
| | - Sang-Bin Lim
- 3 Department of Food Bioengineering, Jeju National University , Jeju, Korea
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Di Lorenzo F, Silipo A, Molinaro A, Parrilli M, Schiraldi C, D’Agostino A, Izzo E, Rizza L, Bonina A, Bonina F, Lanzetta R. The polysaccharide and low molecular weight components of Opuntia ficus indica cladodes: Structure and skin repairing properties. Carbohydr Polym 2017; 157:128-136. [DOI: 10.1016/j.carbpol.2016.09.073] [Citation(s) in RCA: 42] [Impact Index Per Article: 5.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/27/2016] [Revised: 09/15/2016] [Accepted: 09/23/2016] [Indexed: 01/20/2023]
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46
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Extraction and Physicochemical Characterization of Mucilage from Opuntia cochenillifera (L.) Miller. J CHEM-NY 2017. [DOI: 10.1155/2017/4301901] [Citation(s) in RCA: 39] [Impact Index Per Article: 4.9] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/18/2022] Open
Abstract
The aim of this study was to extract mucilage from O. cochenillifera (L.) cacti and determine its functional and physicochemical properties. The best mucilage yield (31%) was obtained by nonthermal extraction with hydration. The mucilage has appreciable carbohydrate and protein contents. The phytochemical analysis shown the presence of alkaloids and terpenes/steroids. The Fourier transformed infrared (FT-IR) spectrum of the mucilage exhibits typical bands for carbohydrates as O–H, C–H, and –COO−. The mucilage demonstrated water- and oil-holding capacities of 2.78 g water/g dry mucilage and 1.80 g oil/g dry mucilage, respectively, these properties can have a positive effect on the texture of the products when used as a stabilizer. The mildly acidic pH (4.8–5) contributes to its emulsifying capacity. The presence of electrolytes in the mucilage can be of great value in flocculation processes. The mucilage forms low viscosity solutions in the same manner as gum Arabic. Finally, its potential for use as a textile dye remover was evaluated, achieving a 70% removal rate from aqueous solutions. The prepared mucilage exhibits properties that recommend it as a natural material that can be used as an additive in the chemical, food, pharmaceuticals and cosmetics industries, as well as in decontamination processes.
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Microbiological Quality of Fresh Nopal Juice. Microorganisms 2016; 4:microorganisms4040046. [PMID: 27973398 PMCID: PMC5192529 DOI: 10.3390/microorganisms4040046] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/24/2016] [Revised: 11/22/2016] [Accepted: 12/01/2016] [Indexed: 12/04/2022] Open
Abstract
The consumption of fresh nopal cactus juice is widely popular among health-conscious consumers in Mexico. The juice is prepared from fresh cladodes that have only been rinsed with tap water and are not subjected to a pasteurization or terminal bacterial reduction process. The aim of this study was to evaluate the microbial quality of commercially available fresh juices (n = 162) made with nopal in Texcoco, State of Mexico, during the summer and spring season. Standard microbiological methods, the PCR technique and the serological method were used for isolation and identification of bacteria. All samples contained total coliforms and 91% were positive for Escherichia coli. Although total coliforms and E. coli were detected throughout the study, their populations were significantly lower (p < 0.05) in winter and spring, respectively. Citrobacter youngae was found in 20% of the samples, an unidentified species of Citrobacter in 10%, C. freundii and Proteus mirabilis in 3%, and Salmonella Javiana in 1%. The presence of these microorganisms, especially Salmonella, in the nopal juices is unacceptable due to its health significance. The information generated in this study is relevant for human health risk assessment associated with the consumption of unpasteurized nopal juices and potential interventions to minimize pathogen contamination.
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Kalegowda P, Chauhan AS, Nanjaraj Urs SM. Opuntia dillenii (Ker-Gawl) Haw cladode mucilage: Physico-chemical, rheological and functional behavior. Carbohydr Polym 2016; 157:1057-1064. [PMID: 27987807 DOI: 10.1016/j.carbpol.2016.10.070] [Citation(s) in RCA: 50] [Impact Index Per Article: 5.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/06/2016] [Revised: 10/01/2016] [Accepted: 10/23/2016] [Indexed: 01/04/2023]
Abstract
The yield of mucilage extracted from cladodes of Opuntia dillenii (Ker-Gawl) Haw in aqueous medium was 6.2%. The neutral sugar comprised of arabinose (38.80%), galactose (33.00%), rhamnose (15.70%), xylose (5.10%), and glucose (5.10%). The mucilage showed pseudo plastic behavior with good swelling index (20%), water holding capacity (g water/g dry sample; 4±0.10) and micrometric properties. In addition, mucilage presented intrinsic viscosity of 3.7 dL/g with average molecular weight of 1.9×103kDa. The FTIR and NMR spectra of extracted mucilage showed characteristic polysaccharide nature. Further, the mucilage exhibited anti-obesity property through lipase inhibition. These findings could highlight that isolated mucilage could be exploited as an additive in food and pharmaceutical sector.
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Affiliation(s)
- Pavithra Kalegowda
- Department of Fruit and Vegetable Technology, CSIR-Central Food Technological Research Institute, Mysuru -570 020, Karnataka, India
| | - Attar Singh Chauhan
- Department of Fruit and Vegetable Technology, CSIR-Central Food Technological Research Institute, Mysuru -570 020, Karnataka, India
| | - Shashirekha Mysore Nanjaraj Urs
- Department of Fruit and Vegetable Technology, CSIR-Central Food Technological Research Institute, Mysuru -570 020, Karnataka, India.
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49
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Damasceno GADB, Silva RMADC, Fernandes JM, Ostrosky EA, Langassner SMZ, Ferrari M. Use of Opuntia ficus-indica (L.) Mill extracts from Brazilian Caatinga as an alternative of natural moisturizer in cosmetic formulations. BRAZ J PHARM SCI 2016. [DOI: 10.1590/s1984-82502016000300012] [Citation(s) in RCA: 8] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/22/2023] Open
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50
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Mbosso Teinkela JE, Siwe Noundou X, Nguemfo EL, Meyer F, Djoukoue A, Van Antwerpen P, Ngouela S, Tsamo E, Mpondo Mpondo EA, Vardamides JC, Azebaze GAB, Wintjens R. Identification of compounds with anti-proliferative activity from the wood of Ficus elastica Roxb. ex Hornem. aerial roots. Fitoterapia 2016; 112:65-73. [PMID: 27167182 DOI: 10.1016/j.fitote.2016.05.002] [Citation(s) in RCA: 8] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/31/2016] [Revised: 05/04/2016] [Accepted: 05/06/2016] [Indexed: 11/16/2022]
Affiliation(s)
- Jean Emmanuel Mbosso Teinkela
- Laboratory of Biopolymers and Supramolecular Nanomaterials, Faculté de Pharmacie, Université Libre de Bruxelles (ULB), Campus Plaine (CP 206/4), Boulevard du Triomphe, B-1050 Brussels, Belgium; Département des Sciences Biologiques, Faculté de Médecine et des Sciences Pharmaceutiques (FMSP), Université de Douala, BP 2701 Douala, Cameroun; Department of Chemistry, Faculty of Sciences, University of Douala, P.O. Box. 24157, Douala, Cameroun.
| | - Xavier Siwe Noundou
- Nanomaterials and Medecinal Organic Chemistry Laboratory, Department of Chemistry, Rhodes University, Grahamstown 6140, South Africa
| | - Edwige Laure Nguemfo
- Département des Sciences Biologiques, Faculté de Médecine et des Sciences Pharmaceutiques (FMSP), Université de Douala, BP 2701 Douala, Cameroun
| | - Franck Meyer
- Laboratory of Biopolymers and Supramolecular Nanomaterials, Faculté de Pharmacie, Université Libre de Bruxelles (ULB), Campus Plaine (CP 206/4), Boulevard du Triomphe, B-1050 Brussels, Belgium
| | - Alfred Djoukoue
- Department of Chemistry, Faculty of Sciences, University of Douala, P.O. Box. 24157, Douala, Cameroun; Département de Pharmacie, Faculté de Médecine et des Sciences Pharmaceutiques (FMSP), Université de Douala, BP 2701 Douala, Cameroun
| | - Pierre Van Antwerpen
- Analytical Platform of the Faculty of Pharmacy, Faculté de Pharmacie, Université Libre de Bruxelles (ULB), Campus Plaine (CP 205/5), Boulevard du Triomphe, B-1050 Brussels, Belgium
| | - Silvère Ngouela
- Laboratoire de Substances Naturelles et Synthèse Organique, Département de Chimie Organique, Faculté des Sciences, Université de Yaoundé I, BP 812 Yaoundé, Cameroun
| | - Etienne Tsamo
- Laboratoire de Substances Naturelles et Synthèse Organique, Département de Chimie Organique, Faculté des Sciences, Université de Yaoundé I, BP 812 Yaoundé, Cameroun
| | - Emmanuel Albert Mpondo Mpondo
- Département de Pharmacie, Faculté de Médecine et des Sciences Pharmaceutiques (FMSP), Université de Douala, BP 2701 Douala, Cameroun
| | | | | | - René Wintjens
- Laboratory of Biopolymers and Supramolecular Nanomaterials, Faculté de Pharmacie, Université Libre de Bruxelles (ULB), Campus Plaine (CP 206/4), Boulevard du Triomphe, B-1050 Brussels, Belgium
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