• antioxidant activity
• biochemical parameters
• diet leaf mixture
• gut histomorphology
• histochemistry
• laurel
• laying hens
• olive
• rosemary
• synergism

• 5003778 European Union, European Regional Development Funds and by National Funds of Greece and Italy, Interreg V-A Greece-Italy 2014-2020

• Antioxidant and anti-inflammatory parameters
• Biochemical
• Diet leaf mixture
• Gut histology
• Laying hens

• Animals
• Chickens/immunology
• Chickens/physiology
• Animal Feed/analysis
• Female
• Dietary Supplements/analysis
• Diet/veterinary
• Plant Leaves/chemistry
• Rosmarinus/chemistry
• Olea/chemistry
• Intestines/drug effects
• Intestines/anatomy & histology
• Animal Nutritional Physiological Phenomena/drug effects
• Oxidative Stress/drug effects
• Ovum/drug effects
• Eggs/analysis
• Eggs/standards

• Cardiac fibrosis
• Herbal plant extracts
• Myocardial infarction
• Therapeutic treatments

• Antioxidant enzymes
• CYP2E
• Caspase 3
• Liver; Silymarin
• Mulberry leaves
• Olive leaves
• Paracetamol

• Rats
• Male
• Animals
• Antioxidants/pharmacology
• Acetaminophen/toxicity
• Acetaminophen/analysis
• Morus
• Olea
• Caspase 3
• Cytochrome P-450 CYP2E1
• Rats, Sprague-Dawley
• Chemical and Drug Induced Liver Injury, Chronic
• Oxidative Stress
• Liver
• Silymarin
• Plant Leaves/chemistry
• Plant Extracts/chemistry
• Chemical and Drug Induced Liver Injury

• antioxidant
• caspase-3
• cytokines
• hepatic fibrosis
• inflammation

Chronic pancreatitis (CP) is an inflammatory disease affects the pancreas with upcoming fibrosis and notable parenchymal destruction. CP poses a high risk for pancreatic carcinoma. The present study aimed to investigate, for the first time up to our knowledge, the effect of olive leaf extract on L-arginine induced CP with referral to some of its underlying mechanisms. Forty adult male albino rats were divided equally into four groups; control, olive leaf extract treated (200 mg/kg orally once daily), CP group (300 mg L-arginine/100 g body weight intraperitoneally, once daily for 3 weeks then every 3 days for the subsequent 3 weeks), and CP treated with olive leaf extract group. At the end of the experiment, body weight, serum glucose, serum insulin, homeostatic model assessment of insulin resistance (HOMA-IR), serum amylase and lipase as well as tissue superoxide dismutase (SOD), and malondialdehyde (MDA) levels were assessed. Pancreatic tissues were subjected to histological and immuno-histochemical studies. The CP group revealed significant decrease in body weight and increase in serum glucose, serum insulin, HOMA-IR score, serum amylase, and serum lipase levels. Significant increase in MDA level and decrease in SOD level were detected. Marked degenerative changes and fibrosis were detected. Upregulation of alpha smooth muscle actin (α-SMA), transforming growth factor beta (TGF-β), caspase-3, and interleukin-6 (IL-6) immunoreactions were implicated in CP pathogenesis. Olive leaf extract alleviated all the examined parameters via its-antioxidant, anti-inflammatory, and anti-fibrotic properties. Olive leaf extract can protect against CP and restore pancreatic functions.

• Chronic pancreatitis
• Fibrosis
• Olive leaf
• Oxidative stress

• HPLC
• acetylcholinesterase
• alloxan-induced diabetic rat models
• enzyme inhibition
• α-glucosidase

Currently, living organisms are increasingly exposed to many toxic chemicals in the environment. These substances pose a threat to human life, other living organisms and ecosystem. In fact, there is an increasing requirement to search for safe therapeutic sources today. Medicinal plants and natural products have become of great importance globally because of their therapeutic potential and medicinal properties, as well as their availability and the absence of harmful side effects for most of them. The present study was designed to explore the potential protective effect of curcumin (CUR) and thymoquinone (TQ) in male rats exposed to thioacetamide (TAA). The experimental mice were divided into eight groups. Group 1 was served as control. Group 2 was exposed to 50 mg/ kg body weight of TAA. Group 3 was exposed to CUR and TAA. Mice of group 4 were treated with TQ and TAA. Mice of group 5 were exposed to CUR plus TQ and TAA. Group 6 was supplemented with CUR. Group 7 was subjected to TQ. Mice of group 8 were treated with CUR and TQ. Hematological and biochemical alterations were evaluated after one month. Significant increases of white blood corpuscles (WBC), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (TB), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) values were observed in group 2, while the values of red blood corpuscles (RBC), hemoglobin (Hb(, hematocrit (Hct), glutathione (GSH) and superoxide dismutase (SOD) were statistically decreased. Treatment with CUR, TQ and their combination inhibited the hematological and biochemical alterations induced by TAA toxicity. Moreover, the most protective effect was observed in mice treated with CUR plus TQ. These new results suggested that the protective effect of CUR and TQ attributed to their antioxidant properties.

• Antioxidant
• Blood
• Curcumin
• Mice
• Thioacetamide
• Thymoquinone

• Rosmarinic acid
• Rosmarinus officinalis
• antidote
• antioxidant
• rosemary
• toxin

• anti-tumor
• antioxidant
• dietary agents
• natural products

• Animals
• Anticarcinogenic Agents/therapeutic use
• Antioxidants/therapeutic use
• Beverages
• Cell Line, Tumor
• Diet
• Dietary Supplements/adverse effects
• Fruit
• Humans
• Neoplasms/metabolism
• Neoplasms/prevention & control
• Oxidative Stress/drug effects
• Plant Preparations/adverse effects
• Plant Preparations/therapeutic use
• Reactive Oxygen Species
• Vegetables

Virgin olive oil has demonstrated its effective activity against oxidative stress. However, data on the bioactive effect of olive leaves or their major constituents on the liver are scarce. The present research work was conducted to evaluate the hepatoprotective effects of supercritical carbon dioxide (SC-CO₂) extracts from fresh and dried olive leaves on hepatotoxicity caused by carbon tetrachloride (CCl₄) in rat models. For this purpose, healthy albino rats of 180–250 g weight were used. The assessment of biochemical markers was carried out on blood and liver tissue. Then, a histopathological study was carried out on liver tissue. The obtained results showed that fresh and dried olive leaf extracts ameliorate the perturbed biochemical parameters caused by CCl₄ treatment. Furthermore, the results registered for the histopathological study are in accordance with the biochemical parameters and the protective capacity of SC-CO₂ extracts against DNA damage, indicating that olive leaf extracts helped to improve liver fibrosis caused by CCl₄ treatment.

• antioxidants
• olive leaves
• supercritical fluid extraction

• Rosmarinus
• extraction techniques
• food applications
• phytoconstituents

• Cichorium intybus linn
• farnesoid-X-receptor (FXR)
• pAMPK
• proliferating cell nuclear antigen (PCNA)
• sirtuin 1 (SIRT1)
• thioacetamide (TAA)

• Adenylate Kinase/genetics
• Adenylate Kinase/metabolism
• Animals
• Body Weight/drug effects
• Cell Proliferation/drug effects
• Chemical and Drug Induced Liver Injury/drug therapy
• Cichorium intybus
• Dietary Supplements
• Gene Expression Regulation/drug effects
• Hepatocytes/drug effects
• Inflammation
• Liver/drug effects
• Liver/pathology
• Liver Cirrhosis/chemically induced
• Liver Cirrhosis/diet therapy
• Male
• Organ Size
• Oxidation-Reduction
• Proliferating Cell Nuclear Antigen
• RNA, Messenger/genetics
• RNA, Messenger/metabolism
• Rats
• Receptors, Cytoplasmic and Nuclear/genetics
• Receptors, Cytoplasmic and Nuclear/metabolism
• Signal Transduction/drug effects
• Sirtuin 1/genetics
• Sirtuin 1/metabolism
• Thioacetamide/toxicity

• Flaxseed oil
• Rats
• Renal toxicity
• Thioacetamide

• antimicrobial
• antioxidant
• phenolic compounds
• rosemary

Juniperus spp. are used as medicinal plants in many countries like Bosnia, Lebanon, and Turkey. In folk medicines, these plants have been used for treating skin and respiratory tract diseases, urinary problems, rheumatism and gall bladder stones. The objectives of this work were to synthesize silver nanoparticles (AgNPs) using a coniferous tree, Juniperus procera leaf extract and testing the synthesized AgNPs for its antimicrobial potentials, hemolytic activity, toxicity and the proliferative effects against normal and activated rat splenic cells. Leaf extract was prepared using acetone and ethanol as solvents. AgNPs were prepared using the acetone extract. AgNPs were validated using UV–Vis spectroscopy and scanning electron microscopy (SEM). Functional groups in the extract were identified using Fourier Transform Infrared (FT-IR) spectroscopy. SEM images of AgNPs showed spherical and cubic shapes with a uniform size distribution with an average size of 30–90 nm. FT-IR spectroscopy showed the presence of many functional groups in the plant extract. AgNPs showed promising antimicrobial activity against tested bacteria and fungus. AgNPs also expressed a stimulating activity towards the rat splenic cells in a dose dependent manner. Acetone as solvent was safer on cells than ethanol. Green synthesized AgNPs using J. procera might be used as a broad-spectrum therapeutic agent against microorganisms and as an immunostimulant agent.

• AgNPs
• Antibacterial
• Antifungal
• Juniperus procera
• Plant extract
• Splenic cells proliferation

• Apoptosis
• Inflammation
• Olive
• Oxidative stress
• Prozac

To explore the relationship of liver and spleen shear wave velocity in patients with liver cirrhosis combined with portal hypertension, and assess the value of liver and spleen shear wave velocity in predicting the prognosis of patients with portal hypertension.

All 67 patients with liver cirrhosis diagnosed as portal hypertension by hepatic venous pressure gradient in our hospital from June 2014 to December 2014 were enrolled into this study. The baseline information of these patients was recorded. Furthermore, 67 patients were followed-up at 20 mo after treatment, and liver and spleen shear wave velocity were measured by acoustic radiation force impulse at the 1^st week, 3^rd month and 9^th month after treatment. Patients with favorable prognosis were assigned into the favorable prognosis group, while patients with unfavorable prognosis were assigned into the unfavorable prognosis group. The variation and difference in liver and spleen shear wave velocity in these two groups were analyzed by repeated measurement analysis of variance. Meanwhile, in order to evaluate the effect of liver and spleen shear wave velocity on the prognosis of patients with portal hypertension, Cox’s proportional hazard regression model analysis was applied. The ability of those factors in predicting the prognosis of patients with portal hypertension was calculated through receiver operating characteristic (ROC) curves.

The liver and spleen shear wave velocity in the favorable prognosis group revealed a clear decline, while those in the unfavorable prognosis group revealed an increasing tendency at different time points. Furthermore, liver and spleen shear wave velocity was higher in the unfavorable prognosis group, compared with the favorable prognosis group; the differences were statistically significant (P < 0.05). The prognosis of patients with portal hypertension was significantly affected by spleen hardness at the 3^rd month after treatment [relative risk (RR) = 3.481]. At the 9^th month after treatment, the prognosis was affected by liver hardness (RR = 5.241) and spleen hardness (RR = 7.829). The differences between these two groups were statistically significant (P < 0.05). The ROC analysis revealed that the area under the curve (AUC) of spleen hardness at the 3^rd month after treatment was 0.644, while the AUCs of liver and spleen hardness at the 9^th month were 0.579 and 0.776, respectively. These might predict the prognosis of patients with portal hypertension.

Spleen hardness at the 3^rd month and liver and spleen shear wave velocity at the 9^th month may be used to assess the prognosis of patients with portal hypertension. This is hoped to be used as an indicator of predicting the prognosis of patients with portal hypertension.

• Liver cirrhosis
• Portal hypertension
• Liver and spleen shear wave velocity
• Acoustic radiation force impulse

• Adult
• Aged
• Elasticity Imaging Techniques/methods
• Feasibility Studies
• Female
• Follow-Up Studies
• Humans
• Hypertension, Portal/complications
• Hypertension, Portal/diagnostic imaging
• Hypertension, Portal/epidemiology
• Incidence
• Liver/diagnostic imaging
• Liver Cirrhosis/diagnostic imaging
• Liver Cirrhosis/epidemiology
• Liver Cirrhosis/etiology
• Liver Cirrhosis/therapy
• Male
• Middle Aged
• Prognosis
• ROC Curve
• Spleen/diagnostic imaging

Medicinal plants have always been an important source of new alternative effective compounds for human therapy. Currently, there are many of scientific evidences indicate that the medicinal plants contain a lot of hypoglycemic chemical compounds. The purpose of the present study was to determine the influence of olive leaves extract on hepatorenal injury in diabetic male rats. Experimental diabetes was induced by streptozotocin (STZ). The levels of serum glucose, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, gamma glutamyl transferase, total bilirubin, creatinine, blood urea nitrogen, uric acid and malondialdehyde were significantly increased, while the levels of serum superoxide dismutase, glutathione and catalase were statistically decreased in untreated diabetic rats. Moreover, the histopathological examination showed several alterations in the structure of liver and kidney in untreated diabetic rats. Treatments with low dose and high dose of olive leaves extract in diabetic rats showed remarkable reducing and protecting influences of physiological and histopathological alterations. Moreover, the highly treatment efficiency was noted in diabetic rats treated with high dose followed by low dose of olive leaves extract. Additionally, the results of this study proved that the antioxidant activities of olive leaves extract played a vital role against the hepatorenal injury induced by diabetes. Finally, this study indicates to the importance of the use of olive leaves extract as promising alternative and complementary therapeutic agent against diabetes and its complications.

• Blood
• Diabetes
• Kidney
• Liver
• Olive leaves
• Rats
• Streptozotocin

The liver modulates several important roles, such as metabolism and liver cirrhosis, which have several cardiovascular problems. Due to preservative role of antioxidant agents in cardiovascular disease, consequently, many of them are applied as medicinal plants in traditional medicine. Vanillic acid (VA), as an antioxidant agent, has a principal preservative role on some diseases. In this study, the effect of vanillic acid was examined on heart rate (as chronotropic property), P-R interval (as dromotropic property), and serum bilirubin in cholestasis-induced model rats.

In this study, 32 male Sprague-Dawley rats weighing 200–250 g were allocated into four groups, and each group contained eight rats as follows: Control (normal saline, 1 ml/kg, gavage, daily for 4 weeks), cirrhotic (normal saline, 1 ml/kg, gavage, daily for 4 weeks), vanillic acid (10 mg/kg, gavage, daily for 4 weeks), cirrhotic treated with vanillic acid (10 mg/kg, gavage, daily for 4 weeks). Chronic biliary cirrhosis was induced in cirrhotic groups by four weeks Bile Duct Ligation (BDL). At the first day and four weeks after surgery, the animals were anesthetized, electrocardiograms were recorded (lead II), and chronotropic and dromotropic properties (HR and PR interval) were investigated. At the end of experimental duration, the animals were anesthetized, and blood samples were taken to measure serum bilirubin. The results were analyzed using t-test and one-way ANOVA by SPSS software, version 22.

After induced of BDL, the results presented that laboratory parameter (bilirubin) in the cirrhotic group significantly increased compared to the control group. The P-R interval was reduced in the cirrhotic group compared to the control group, and there was no significant difference between heart rate in all groups. Bilirubin were reduced in cirrhotic groups treated with vanillic acid (VA) compared to cirrhotic group and also administration of VA in the cirrhotic treated with VA increased dromotropic property in comparison with the cirrhotic group.

According to the results obtained in this study, preventing elevated bilirubin and increase dromotropic property in cirrhotic group taking the VA suggested that the consumption of vanillic acid as an antioxidant can be effective in the prevention of liver diseases.

• biliary cirrhosis
• bilirubin
• chronotropic
• dromotropic
• rat
• vanillic acid

The current study was designed to investigate the possible protective effect of omega-3 fatty acids from fish oil on hepatic fibrosis induced by thioacetamide (TAA) in male rats. The experimental animals were divided into four groups. The first group was received saline solution and served as control. The second group was given 250 mg/kg body weight of TAA. The third group was treated with omega-3 fatty acids and TAA. The fourth group was given saline solution and supplemented with omega-3 fatty acids. Treatment of rats with TAA for three and six weeks resulted in a significant decrease in body weight gain, while the value of liver/body weight ratio was statistically increased. Furthermore, the levels of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, gamma glutamyl transferase and total bilirubin were significantly increased. After three weeks of exposure to only TAA, liver sections showed an abnormal morphology characterized by noticeable fibrosis with the extracellular matrix collagen contents and damage of liver cells’ structure. Liver sections from rats treated with only TAA for six weeks revealed an obvious increase in extracellular matrix collagen content and bridging fibrosis. Treating TAA-intoxicated rats with omega-3 fatty acids significantly attenuated the severe physiological and histopathological changes. Finally, the present investigation suggests that omega-3 fatty acids could act against hepatic fibrosis induced by TAA due to its antioxidant properties, thus supporting its use in hepatic fibrosis therapy.

• Hepatic fibrosis
• Omega-3 fatty acids
• Rats
• Thioacetamide

The effect of Olea oleaster and Juniperus procera leaves extracts and their combination on thioacetamide (TAA)-induced hepatic cirrhosis were investigated in male albino mice. One hundred sixty mice were used in this study and were randomly distributed into eight groups of 20 each. Mice of group 1 served as controls. Mice of group 2 were treated with TAA. Mice of group 3 were exposed to TAA and supplemented with O. oleaster leaves extracts. Mice of group 4 were treated with TAA and supplemented with J. procera leaves extracts. Mice of group 5 were subjected to TAA and supplemented with O. oleaster and J. procera leaves extracts. Mice of groups 6, 7 and 8 were supplemented with O. oleaster, J. procera, and O. oleaster and J. procera leaves extracts respectively. Administration of TAA for six and twelve weeks resulted in a decline in body weight gain and increased the levels of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and total bilirubin. Histopathological evaluations of hepatic sections from mice treated with TAA showed severe alterations including increase of fibrogenesis processes with structural damage. Treatment of mice with these extracts showed a pronounced attenuation in TAA induced hepatic cirrhosis associated with physiological and histopathological alterations. Finally, this study suggests that the supplementation of these extracts may act as antioxidant agents and could be an excellent adjuvant support in the therapy of hepatic cirrhosis.

• Hepatic cirrhosis
• Juniperus procera
• Mice
• Olea oleaster
• Thioacetamide