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Romig T, Wassermann M. Echinococcus species in wildlife. Int J Parasitol Parasites Wildl 2024; 23:100913. [PMID: 38405672 PMCID: PMC10884515 DOI: 10.1016/j.ijppaw.2024.100913] [Citation(s) in RCA: 11] [Impact Index Per Article: 11.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/15/2023] [Revised: 01/31/2024] [Accepted: 02/01/2024] [Indexed: 02/27/2024]
Abstract
Transmission of Echinococcus spp. in life cycles that involve mainly wildlife is well recognized for those species with small mammals as intermediate hosts (e. g. E. multilocularis), as well as for E. felidis and the 'northern' genotypes of E. canadensis (G8 and G10). In contrast, the remaining taxa of E. granulosus sensu lato are best known for their domestic life cycles, and the numerous wild mammal species (mainly ungulates) that have been recorded with cystic echinococcosis in the past were mainly considered a result of spill-over from the dog-livestock transmission system. This view was challenged with the advent of molecular characterization, allowing discrimination of the metacestodes, although the contribution of wild mammals to various Echinococcus life cycles has remained uncertain for scarcity of wildlife studies. Numerous records of cysts in wild ungulates date back to the 20th century, but cannot with certainty be allocated to the Echinococcus species and genotypes that are recognized today. This means that our current knowledge is largely restricted to studies of the past two decades that kept adding gradually to our concepts of transmission in various geographic regions. In particular, new insights were gathered in the past years on E. granulosus s.l. in wildlife of sub-Saharan Africa, but also on transmission patterns of E. multilocularis in previously neglected regions, e. g. North America. Here, an update is provided on the current state of knowledge on wild mammals as hosts for all Echinococcus species, listing >150 species of wild hosts with references, as well as estimates on their epidemiological impact and our current gaps of knowledge.
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Affiliation(s)
- Thomas Romig
- University of Hohenheim, Parasitology Unit, 70599, Stuttgart, Germany
- University of Hohenheim, Center for Biodiversity and Integrative Taxonomy, 70599, Stuttgart, Germany
| | - Marion Wassermann
- University of Hohenheim, Parasitology Unit, 70599, Stuttgart, Germany
- University of Hohenheim, Center for Biodiversity and Integrative Taxonomy, 70599, Stuttgart, Germany
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Zanini F, Di Salvo V, Pierangeli N, Lazzarini L, Curto E. Presence of Echinococcus granulosussensulato in the endoparasitic fauna of feral dogs in Tierra del Fuego, Argentina. Vet Parasitol Reg Stud Reports 2023; 44:100916. [PMID: 37652635 DOI: 10.1016/j.vprsr.2023.100916] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/18/2023] [Revised: 06/10/2023] [Accepted: 07/26/2023] [Indexed: 09/02/2023]
Abstract
The feral dog population from Tierra del Fuego (Argentina) has affected provincial sheep production, and is responsible for the decrease of 43% of the sheep stock. In addition, feral dogs preys on the native fauna, especially the guanaco (Lama guanicoe), a species protected by law. Except for some data on the aforementioned impact, very little is known about feral dogs features in this region, such as distribution, abundance, diet, reproduction, health status, among others. Therefore, a descriptive observational study was carried out in the period 2018-2021 in Tierra del Fuego, with the aim to recognize the endoparasitic fauna of these dogs, with emphasis on Echinococcus granulosus sensu lato (s.l.). Feces samples were collected from 83 feral dogs captured in 15 farms, which were evaluated using flotation and sedimentation coproparasitological techniques, and optical microscope observation. These qualitative methods showed that 66.3% of the samples were positive. Nine parasitic forms were found, including eggs of taenids, Toxocara canis and Toxascaris leonina, and Sarcocystis spp. oocysts and Giardia spp. cysts. Samples with more than one parasitic form were also observed, among which the Taenia/Sarcocystis spp. association was the most frequent. For the diagnosis of canine echinococcosis, the detection of Echinococcus spp. antigens by Copro-ELISA and the molecular confirmation by Copro-PCR were performed. The Copro-ELISA test was positive in 10/80 (12.5%) of the samples processed, while 11 samples (13.8%) were positive by Copro-PCR. Their distribution showed that 9 (81.8%) corresponded to farms with sheep farming, and 2 (18.2%) with cattle farming. The presence of E. granulosus s.l. in feral dogs from Tierra del Fuego is the most relevant finding in this study. These results confirm that feral dogs have access to viscera infected with hydatid cysts. However, the intermediate hosts involved (sheep, cattle or guanacos) should be investigated. Its impact on the transmission of cystic echinococcosis in this region should be evaluated, as well as the adequacy of the disease prevention and control actions implemented in Tierra del Fuego should be considered. Finally, the existence of a wild cycle of the disease should be evaluated, in which feral dogs would act as a source of infection for other animals and humans.
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Affiliation(s)
- Fabián Zanini
- Private Activity in Veterinary Medicine, Berta Weber 79 (9420) Rio Grande, Provincia de Tierra del Fuego, AeIAS, Argentina
| | - Vilma Di Salvo
- Laboratorio de Sanidad Animal Provincial "Dr. Raúl Chifflet", Ministerio de Producción y Ambiente, Gobierno de Tierra del Fuego, AeIAS, El Esquiador 138 (9420) Río Grande, Provincia de Tierra del Fuego, AeIAS, Argentina
| | - Nora Pierangeli
- Cátedra de Microbiología y Parasitología, Facultad de Ciencias Médicas, Universidad Nacional del Comahue, Irigoyen 2000 (8324) Cipolletti, Provincia de Río Negro, Argentina.
| | - Lorena Lazzarini
- Cátedra de Microbiología y Parasitología, Facultad de Ciencias Médicas, Universidad Nacional del Comahue, Irigoyen 2000 (8324) Cipolletti, Provincia de Río Negro, Argentina
| | - Erio Curto
- Secretaría de Ambiente, Desarrollo Sostenible y Cambio Climático de la Provincia de Tierra del Fuego, Antártida e Islas del Atlántico Sur. San Martín 1401 (V9410BGB) Ushuaia, Provincia de Tierra del Fuego, AeIAS, Argentina
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Kurcheid J, Gordon CA, Clarke NE, Wangdi K, Kelly M, Lal A, Mutombo PN, Wang D, Mationg ML, Clements AC, Muhi S, Bradbury RS, Biggs BA, Page W, Williams G, McManus DP, Gray D. Neglected tropical diseases in Australia: a narrative review. Med J Aust 2022; 216:532-538. [PMID: 35560239 DOI: 10.5694/mja2.51533] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/19/2021] [Revised: 10/25/2021] [Accepted: 11/02/2021] [Indexed: 11/28/2024]
Abstract
•Neglected tropical diseases (NTDs) represent a threat to the health, wellbeing and economic prosperity of billions of people worldwide, often causing serious disease or death. •Commonly considered diseases of low and middle-income nations, the presence of NTDs in high income countries such as Australia is often overlooked. •Seven of the 20 recognised NTDs are endemic in Australia: scabies, soil-transmitted helminths and strongyloidiasis, echinococcosis, Buruli ulcer, leprosy, trachoma, and snakebite envenoming. •Dengue, while not currently endemic, poses a risk of establishment in Australia. There are occasional outbreaks of dengue fever, with local transmission, due to introductions in travellers from endemic regions. •Similarly, the risk of introduction of other NTDs from neighbouring countries is a concern. Many NTDs are only seen in Australia in individuals travelling from endemic areas, but they need to be recognised in health settings as the potential consequences of infection can be severe. •In this review, we consider the status of NTDs in Australia, explore the risk of introducing and contracting these infections, and emphasise the negative impact they have on the health of Australians, especially Aboriginal and Torres Strait Islander peoples.
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Affiliation(s)
- Johanna Kurcheid
- Australian National University, Canberra, ACT
- Swiss Tropical and Public Health Institute, Basel, Switzerland
| | | | - Naomi E Clarke
- Australian National University, Canberra, ACT
- Kirby Institute, University of New South Wales, Sydney, NSW
| | | | | | - Aparna Lal
- Australian National University, Canberra, ACT
| | - Polydor N Mutombo
- National Centre for Naturopathic Medicine, Southern Cross University, Lismore, NSW
| | - Dongxu Wang
- Australian National University, Canberra, ACT
| | | | | | - Stephen Muhi
- Victorian Infectious Diseases Service, Royal Melbourne Hospital, Melbourne, VIC
| | | | - Beverley-Ann Biggs
- Victorian Infectious Diseases Service, Royal Melbourne Hospital, Melbourne, VIC
| | | | | | | | - Darren Gray
- Australian National University, Canberra, ACT
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Hombo H, Oyarzo M, Álvarez C, Cuadros N, Hernández F, Ward MP, Acosta-Jamett G. Spatial epidemiology of cystic echinococcosis in livestock from a hyper-endemic region in southern Chile. Vet Parasitol 2020; 287:109258. [PMID: 33075729 DOI: 10.1016/j.vetpar.2020.109258] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/29/2020] [Revised: 09/26/2020] [Accepted: 09/27/2020] [Indexed: 10/23/2022]
Abstract
Cystic echinococcosis (CE) is a parasitic zoonosis, caused by the larval stage of Echinococcus granulosus. This disease has a worldwide distribution and is considered an important zoonosis in Chile, especially in regions where raising livestock dominates. We aimed to describe the spatial distribution and risk factors for CE in cattle and sheep slaughtered in the Aysén Region, Chile between 2015 and 2016, to inform disease control in this hyper-endemic region. Clustering and hotspot analyses of CE at the farm-level were performed (Moran's global index, local indicator of spatial autocorrelation (LISA), and the scan statistic Poisson model), and we used spatial interpolation to display areas with a higher risk of CE. Mixed effects logistic regression models were fit to assess the association between municipality of origin and age of animals and CE detected at slaughter, with the farm of origin included as a random effect. Overall, data from 1532 cattle farms (1078 geocoded) and 30,805 cattle were analyzed, with a 64 % and 39 % CE prevalence at the farm and animal-level, respectively. For sheep farms, data from 381 (296 geocoded) farms and 58,223 sheep were analyzed, with a 71 % and 18 % CE prevalence at the farm and animal-level, respectively. Globally, the CE prevalence in both cattle and sheep farms was not clustered. However, we found 14 cattle and 23 sheep farms with high prevalence that were locally clustered. We also detected 6 and 11 clusters in cattle and sheep farms, respectively. Risk of reported CE varied according to municipalities of origin, and the age of animals. This study confirms that the Aysén region is a hyper-endemic region for CE. It demonstrates the usefulness of the traceability of animals slaughtered for understanding the epidemiology of CE. The results suggest that in this hyper-endemic region in which there are limited financial resources available, that intervention activities - at the beginning of a control program - should be focused on farms with high prevalence and identified disease clusters.
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Affiliation(s)
- Hamilton Hombo
- Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile; Escuela de Graduados, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile
| | - Miguel Oyarzo
- Unidad de Zoonosis, Secretaría Regional Ministerial de Salud, Región de Aysén, Coyhaique, Chile
| | - Claudia Álvarez
- Unidad de Zoonosis, Secretaría Regional Ministerial de Salud, Región de Aysén, Coyhaique, Chile
| | - Natalia Cuadros
- Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile
| | - Felipe Hernández
- Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile
| | - Michael P Ward
- Sydney School of Veterinary Science, The University of Sydney, 425 Werombi Road, Private Mailbag 4003, Narellan, NSW, 2567, Australia
| | - Gerardo Acosta-Jamett
- Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile; Programa de investigación Aplicada en Fauna Silvestre, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile.
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Wilson CS, Brookes VJ, Barnes TS, Woodgate RG, Peters A, Jenkins DJ. Revisiting cyst burden and risk factors for hepatic hydatid disease (Echinococcus granulosus sensu stricto) in Australian beef cattle. Prev Vet Med 2019; 172:104791. [PMID: 31627165 DOI: 10.1016/j.prevetmed.2019.104791] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/10/2019] [Revised: 08/07/2019] [Accepted: 10/02/2019] [Indexed: 10/25/2022]
Abstract
The characteristics and risk factors associated with hepatic Echinococcus granulosus sensu stricto infection (hydatid disease) were investigated in beef cattle slaughtered at an abattoir in eastern Australia. Sampled cattle were sourced from all eastern states, predominantly from regions associated with the Great Dividing Range. Livers and corresponding demographic data were collected from 601 carcasses. Livers were examined for the number, size, viability, and fertility of hydatid cysts. Mixed effects logistic regression was used to evaluate associations of sex, feed-type (grass- or grain-fed), and dentition (age) on hydatid disease. Hydatid cysts were detected in all dentition groups. The most commonly sampled dentition group was zero-tooth cattle (less than 18 months). Twenty-nine percent of infected livers had only one cyst, and 48% of infected livers contained viable cysts. Thirty-seven percent of infected livers had cysts that were 3-10 mm in diameter. The size and number of cysts were positively correlated with age of the animal. Regression analysis showed that the odds of hydatid disease were highest in eight-tooth cattle (>42 months; OR 26.9; 95% CI 11.8-61.6; reference level [ref] zero-tooth). Being grass-fed was also significantly associated with the presence of hydatid disease (total effect; OR 3.0; 95% CI 1.7-5.5; ref grain-fed). Although there was no evidence of a total effect of sex across the study population, males of a given dentition group and feed-type (grass- or grain-fed) were more likely to be infected than respective females. Despite changes in Australian agriculture in the last 30 years, the burden (number, size, and viability of cysts) of hydatid disease in individual infected animals remains similar to previous Australian studies.
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Affiliation(s)
- Cara S Wilson
- School of Animal and Veterinary Sciences, Faculty of Science, Charles Sturt University, Wagga Wagga, NSW 2678, Australia; Graham Centre for Agricultural Innovation (NSW Department of Primary Industries and Charles Sturt University), Wagga Wagga, NSW 2650, Australia.
| | - Victoria J Brookes
- School of Animal and Veterinary Sciences, Faculty of Science, Charles Sturt University, Wagga Wagga, NSW 2678, Australia; Graham Centre for Agricultural Innovation (NSW Department of Primary Industries and Charles Sturt University), Wagga Wagga, NSW 2650, Australia; Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, NSW 2570, Australia
| | - Tamsin S Barnes
- The University of Queensland, School of Veterinary Science, Gatton, QLD 4343, Australia; The University of Queensland, Queensland Alliance for Agriculture and Food Innovation, Gatton, QLD 4343, Australia
| | - Rob G Woodgate
- School of Animal and Veterinary Sciences, Faculty of Science, Charles Sturt University, Wagga Wagga, NSW 2678, Australia; Graham Centre for Agricultural Innovation (NSW Department of Primary Industries and Charles Sturt University), Wagga Wagga, NSW 2650, Australia
| | - Andrew Peters
- School of Animal and Veterinary Sciences, Faculty of Science, Charles Sturt University, Wagga Wagga, NSW 2678, Australia; Graham Centre for Agricultural Innovation (NSW Department of Primary Industries and Charles Sturt University), Wagga Wagga, NSW 2650, Australia
| | - David J Jenkins
- School of Animal and Veterinary Sciences, Faculty of Science, Charles Sturt University, Wagga Wagga, NSW 2678, Australia; Graham Centre for Agricultural Innovation (NSW Department of Primary Industries and Charles Sturt University), Wagga Wagga, NSW 2650, Australia
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Cripps JK, Pacioni C, Scroggie MP, Woolnough AP, Ramsey DSL. Introduced deer and their potential role in disease transmission to livestock in Australia. Mamm Rev 2018. [DOI: 10.1111/mam.12142] [Citation(s) in RCA: 17] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/28/2022]
Affiliation(s)
- Jemma K. Cripps
- Department of Environment, Land, Water and Planning; Arthur Rylah Institute for Environmental Research; Heidelberg Vic. 3084 Australia
- School of BioSciences; University of Melbourne; Melbourne Vic. 3010 Australia
| | - Carlo Pacioni
- Department of Environment, Land, Water and Planning; Arthur Rylah Institute for Environmental Research; Heidelberg Vic. 3084 Australia
- School of BioSciences; University of Melbourne; Melbourne Vic. 3010 Australia
| | - Michael P. Scroggie
- Department of Environment, Land, Water and Planning; Arthur Rylah Institute for Environmental Research; Heidelberg Vic. 3084 Australia
- School of BioSciences; University of Melbourne; Melbourne Vic. 3010 Australia
| | - Andrew P. Woolnough
- Department of Economic Development, Jobs, Transport and Resources; 475 Mickleham Road Attwood Vic. 3049 Australia
| | - David S. L. Ramsey
- Department of Environment, Land, Water and Planning; Arthur Rylah Institute for Environmental Research; Heidelberg Vic. 3084 Australia
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Phythian CJ, Jackson B, Bell R, Citer L, Barwell R, Windsor PA. Abattoir surveillance of Sarcocystis spp., Cysticercosis ovis and Echinococcus granulosus in Tasmanian slaughter sheep, 2007-2013. Aust Vet J 2018; 96:62-68. [PMID: 29479682 DOI: 10.1111/avj.12670] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/27/2016] [Revised: 06/12/2017] [Accepted: 07/31/2017] [Indexed: 11/27/2022]
Abstract
OBJECTIVE To estimate the prevalence of macroscopic Sarcocystis spp., Cysticercus ovis and Echinococcus granulosus recorded at routine postmortem inspection of Tasmanian slaughter sheep during 2007 to 2013. METHODS A retrospective analysis of routine postmortem meat inspection data maintained on 352,325 Tasmanian adult slaughter sheep inspected across nine abattoirs in Tasmania, Victoria and South Australia as part of the National Sheep Health Monitoring Project (NSHMP). RESULTS During the period 1 September 2007 to 30 June 2013, the estimated prevalence of macroscopic Sarcocystis spp. was 14.3%, C. ovis was 3.2% and E. granulosus was 0.01%. Mean Sarcocystis spp. line prevalence ranged from 0% to 33.5%. Significant between-abattoir differences in the level of sarcosporidiosis (P < 0.001) and C. ovis were found (P < 0.001). Overall, very low levels of hydatidosis were recorded throughout the surveillance period. Predicted within-line prevalence of macroscopic sarcocysts in animals coming from a known/recorded local government area (LGA) (P < 0.001) was lower than that of lines where the LGA was unknown or not recorded. A higher prevalence of sarcocystosis was recorded in lines of sheep aged ≥ 2 years compared with those < 2 years (P < 0.001). CONCLUSION Reasons for the significant between-abattoir differences in recorded levels of ovine sarcosporidiosis and cysticercosis remain unknown, but may represent sampling bias, with subsets of slaughter sheep going to abattoirs with different tiers or access to markets. Further investigation into apparent differences, including epidemiological studies of properties with high lesion prevalence, comparing meat inspector diagnostic sensitivity, assessing the effect of line speed and tiers and market access in different abattoirs, may be useful.
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Affiliation(s)
- C J Phythian
- Norwegian University of Life Sciences, Faculty of Veterinary Medicine, Institute for Production Animal Clinical Medicine, Section for Small Ruminants, 4325 Sandnes, Norway
| | - B Jackson
- Biosecurity Tasmania, Department for Primary Industries, Parks, Water and Environment, Tasmanian Government, Launceston, Tasmania
| | - R Bell
- Biosecurity Tasmania, Department for Primary Industries, Parks, Water and Environment, Tasmanian Government, Launceston, Tasmania
| | - L Citer
- Animal Health Australia, Braddon Australia Capital Territory, Australia
| | - R Barwell
- Animal Health Australia, Braddon Australia Capital Territory, Australia
| | - P A Windsor
- University of Sydney, Faculty of Veterinary Science, Camden, New South Wales, Australia
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Kinkar L, Laurimäe T, Acosta-Jamett G, Andresiuk V, Balkaya I, Casulli A, Gasser RB, van der Giessen J, González LM, Haag KL, Zait H, Irshadullah M, Jabbar A, Jenkins DJ, Kia EB, Manfredi MT, Mirhendi H, M'rad S, Rostami-Nejad M, Oudni-M'rad M, Pierangeli NB, Ponce-Gordo F, Rehbein S, Sharbatkhori M, Simsek S, Soriano SV, Sprong H, Šnábel V, Umhang G, Varcasia A, Saarma U. Global phylogeography and genetic diversity of the zoonotic tapeworm Echinococcus granulosus sensu stricto genotype G1. Int J Parasitol 2018; 48:729-742. [PMID: 29782829 DOI: 10.1016/j.ijpara.2018.03.006] [Citation(s) in RCA: 75] [Impact Index Per Article: 10.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/21/2017] [Revised: 03/05/2018] [Accepted: 03/08/2018] [Indexed: 02/07/2023]
Abstract
Echinococcus granulosus sensu stricto (s.s.) is the major cause of human cystic echinococcosis worldwide and is listed among the most severe parasitic diseases of humans. To date, numerous studies have investigated the genetic diversity and population structure of E. granulosus s.s. in various geographic regions. However, there has been no global study. Recently, using mitochondrial DNA, it was shown that E. granulosus s.s. G1 and G3 are distinct genotypes, but a larger dataset is required to confirm the distinction of these genotypes. The objectives of this study were to: (i) investigate the distinction of genotypes G1 and G3 using a large global dataset; and (ii) analyse the genetic diversity and phylogeography of genotype G1 on a global scale using near-complete mitogenome sequences. For this study, 222 globally distributed E. granulosus s.s. samples were used, of which 212 belonged to genotype G1 and 10 to G3. Using a total sequence length of 11,682 bp, we inferred phylogenetic networks for three datasets: E. granulosus s.s. (n = 222), G1 (n = 212) and human G1 samples (n = 41). In addition, the Bayesian phylogenetic and phylogeographic analyses were performed. The latter yielded several strongly supported diffusion routes of genotype G1 originating from Turkey, Tunisia and Argentina. We conclude that: (i) using a considerably larger dataset than employed previously, E. granulosus s.s. G1 and G3 are indeed distinct mitochondrial genotypes; (ii) the genetic diversity of E. granulosus s.s. G1 is high globally, with lower values in South America; and (iii) the complex phylogeographic patterns emerging from the phylogenetic and geographic analyses suggest that the current distribution of genotype G1 has been shaped by intensive animal trade.
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Affiliation(s)
- Liina Kinkar
- Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, Vanemuise 46, 51003 Tartu, Estonia
| | - Teivi Laurimäe
- Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, Vanemuise 46, 51003 Tartu, Estonia
| | - Gerardo Acosta-Jamett
- Instituto de Medicina Preventiva Veterinaria y Programa de Investigación Aplicada en Fauna Silvestre, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile
| | - Vanessa Andresiuk
- Laboratorio de Zoonosis Parasitarias, FCEyN, UNMdP, Funes 3350, CP: 7600 Mar del Plata, Buenos Aires, Argentina
| | - Ibrahim Balkaya
- Department of Parasitology, Faculty of Veterinary Medicine, University of Atatürk, Erzurum, Turkey
| | - Adriano Casulli
- World Health Organization Collaborating Centre for the Epidemiology, Detection and Control of Cystic and Alveolar Echinococcosis, European Union Reference Laboratory for Parasites (EURLP), Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
| | - Robin B Gasser
- Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia
| | - Joke van der Giessen
- Centre for Infectious Disease Control Netherlands, National Institute for Public Health and Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands
| | - Luis Miguel González
- Parasitology Department, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, Madrid 28220, Spain
| | - Karen L Haag
- Departamento de Genética, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, Porto Alegre, RS, Brazil
| | - Houria Zait
- Parasitology and Mycology Department, Mustapha University Hospital, 16000 Algiers, Algeria
| | - Malik Irshadullah
- Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh 202002, India
| | - Abdul Jabbar
- Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia
| | - David J Jenkins
- School of Animal and Veterinary Sciences, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia
| | - Eshrat Beigom Kia
- Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
| | - Maria Teresa Manfredi
- Department of Veterinary Medicine, Università degli Studi di Milano, via Celoria 10, 20133 Milan, Italy
| | - Hossein Mirhendi
- Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
| | - Selim M'rad
- Laboratory of Medical and Molecular Parasitology-Mycology (LP3M), LR 12ES08. Faculty of Pharmacy, University of Monastir, 5000 Monastir, Tunisia
| | - Mohammad Rostami-Nejad
- Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| | - Myriam Oudni-M'rad
- Laboratory of Medical and Molecular Parasitology-Mycology (LP3M), LR 12ES08. Faculty of Pharmacy, University of Monastir, 5000 Monastir, Tunisia
| | - Nora Beatriz Pierangeli
- Department of Microbiology and Parasitology, Faculty of Medical Sciences, Comahue National University, Buenos Aires 1400, 8300 Neuquén, Argentina
| | - Francisco Ponce-Gordo
- Department of Parasitology, Faculty of Pharmacy, Complutense University, Plaza Ramón y Cajal s/n, 28040 Madrid, Spain
| | - Steffen Rehbein
- Merial GmbH, Kathrinenhof Research Center, Walchenseestr. 8-12, 83101 Rohrdorf, Germany
| | - Mitra Sharbatkhori
- Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran
| | - Sami Simsek
- Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119 Elazig, Turkey
| | - Silvia Viviana Soriano
- Department of Microbiology and Parasitology, Faculty of Medical Sciences, Comahue National University, Buenos Aires 1400, 8300 Neuquén, Argentina
| | - Hein Sprong
- Centre for Infectious Disease Control Netherlands, National Institute for Public Health and Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands
| | - Viliam Šnábel
- Institute of Parasitology, Slovak Academy of Sciences, Košice, Hlinkova 3, 040 01 Košice, Slovakia
| | - Gérald Umhang
- ANSES, Nancy Laboratory for Rabies and Wildlife, Wildlife Surveillance and Eco-epidemiology Unit, Malzéville 54220, France
| | - Antonio Varcasia
- Laboratory of Parasitology, Veterinary Teaching Hospital, Department of Veterinary Medicine, University of Sassari, Via Vienna, 2-07100 Sassari, Italy
| | - Urmas Saarma
- Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, Vanemuise 46, 51003 Tartu, Estonia.
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Binns C, Low WY. Barking Our Way Into the Year of the Dog: Public Health Benefits and Challenges. Asia Pac J Public Health 2018; 30:4-6. [PMID: 29338290 DOI: 10.1177/1010539517753476] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/16/2022]
Affiliation(s)
- Colin Binns
- 1 School of Public Health, Curtin University, Perth, Western Australia, Australia
| | - Wah Yun Low
- 2 Research Management Center, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
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Keong B, Wilkie B, Sutherland T, Fox A. Hepatic cystic echinococcosis in Australia: an update on diagnosis and management. ANZ J Surg 2017; 88:26-31. [PMID: 29024292 DOI: 10.1111/ans.14117] [Citation(s) in RCA: 28] [Impact Index Per Article: 3.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/26/2017] [Revised: 05/29/2017] [Accepted: 05/30/2017] [Indexed: 01/22/2023]
Affiliation(s)
- Ben Keong
- Department of Upper Gastrointestinal Surgery; St George Hospital; Sydney New South Wales Australia
| | - Bruce Wilkie
- Department of Surgery; Eastern Health; Melbourne Victoria Australia
| | - Tom Sutherland
- Medical Imaging Department; St Vincent's Hospital; Melbourne Victoria Australia
| | - Adrian Fox
- Department of Surgery; Eastern Health; Melbourne Victoria Australia
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11
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Rashid A, Darzi MM, Mir MS, Dar LM, Mir A, Kashani SB, Maharana BR, Patbandha TK, Allaie IM. Prevalence of ovine cystic echinococcosis in Kashmir Valley, North India. VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS 2017; 10:85-89. [PMID: 31014606 DOI: 10.1016/j.vprsr.2017.08.007] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/17/2016] [Revised: 08/14/2017] [Accepted: 08/15/2017] [Indexed: 11/29/2022]
Abstract
A total of 1455 local and non-local (originating from other Indian states), slaughtered or spontaneously dead, sheep in various areas of Kashmir Valley were investigated for the presence of cystic echinococcosis over a period of one year. The overall prevalence was 7.97% with higher prevalence in local (14.3%) than in non-local sheep (6.06%). The prevalence of infection, total number of cysts recovered and mean intensity of infection were higher in lungs as 66.2%, 506 & 5.1% respectively, followed by liver (28.5%, 169, 3.9%) and spleen (5.3%, 9, 1.13%). Either single (71.55%) or multiple (28.45%) organ involvements were observed. 66.6% of cysts were of small size, 19.29% medium, 7.01% large and 7.01% calcified. The fertility of cysts was noted to be 65.7% whereas 34.2% were infertile which included 27.1% sterile and 7.01% calcified cysts. The viability percentage of protoscolices from all the fertile cysts was 74.2%. The number of cysts recovered was higher in sheep with body condition score- emaciated, thin and average, and lower in, fat and obesed. The study showed that the local sheep were more vulnerable to contract cystic echinococcosis than non-local sheep which is further aggravated by poor body condition.
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Affiliation(s)
- Adil Rashid
- Department of Animal Husbandry, Kashmir, J&K, India
| | - M M Darzi
- Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, J&K, India
| | - M S Mir
- Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, J&K, India
| | - L M Dar
- Department of Animal Husbandry, Kashmir, J&K, India
| | - Ambreen Mir
- Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, J&K, India
| | - Syed Bisma Kashani
- Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, J&K, India
| | - B R Maharana
- College of Veterinary Sciences, Junagadh Agricultural University, Gujarat, India
| | - T K Patbandha
- College of Veterinary Sciences, Junagadh Agricultural University, Gujarat, India
| | - I M Allaie
- Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, J&K, India.
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Bombara CB, Dürr S, Machovsky-Capuska GE, Jones PW, Ward MP. A preliminary study to estimate contact rates between free-roaming domestic dogs using novel miniature cameras. PLoS One 2017; 12:e0181859. [PMID: 28750073 PMCID: PMC5547700 DOI: 10.1371/journal.pone.0181859] [Citation(s) in RCA: 15] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/09/2017] [Accepted: 07/07/2017] [Indexed: 11/22/2022] Open
Abstract
Information on contacts between individuals within a population is crucial to inform disease control strategies, via parameterisation of disease spread models. In this study we investigated the use of dog-borne video cameras–in conjunction with global positioning systems (GPS) loggers–to both characterise dog-to-dog contacts and to estimate contact rates. We customized miniaturised video cameras, enclosed within 3D-printed plastic cases, and attached these to nylon dog collars. Using two 3400 mAh NCR lithium Li-ion batteries, cameras could record a maximum of 22 hr of continuous video footage. Together with a GPS logger, collars were attached to six free roaming domestic dogs (FRDDs) in two remote Indigenous communities in northern Australia. We recorded a total of 97 hr of video footage, ranging from 4.5 to 22 hr (mean 19.1) per dog, and observed a wide range of social behaviours. The majority (69%) of all observed interactions between community dogs involved direct physical contact. Direct contact behaviours included sniffing, licking, mouthing and play fighting. No contacts appeared to be aggressive, however multiple teeth baring incidents were observed during play fights. We identified a total of 153 contacts–equating to 8 to 147 contacts per dog per 24 hr–from the videos of the five dogs with camera data that could be analysed. These contacts were attributed to 42 unique dogs (range 1 to 19 per video) which could be identified (based on colour patterns and markings). Most dog activity was observed in urban (houses and roads) environments, but contacts were more common in bushland and beach environments. A variety of foraging behaviours were observed, included scavenging through rubbish and rolling on dead animal carcasses. Identified food consumed included chicken, raw bones, animal carcasses, rubbish, grass and cheese. For characterising contacts between FRDD, several benefits of analysing videos compared to GPS fixes alone were identified in this study, including visualisation of the nature of the contact between two dogs; and inclusion of a greater number of dogs in the study (which do not need to be wearing video or GPS collars). Some limitations identified included visualisation of contacts only during daylight hours; the camera lens being obscured on occasion by the dog’s mandible or the dog resting on the camera; an insufficiently wide viewing angle (36°); battery life and robustness of the deployments; high costs of the deployment; and analysis of large volumes of often unsteady video footage. This study demonstrates that dog-borne video cameras, are a feasible technology for estimating and characterising contacts between FRDDs. Modifying camera specifications and developing new analytical methods will improve applicability of this technology for monitoring FRDD populations, providing insights into dog-to-dog contacts and therefore how disease might spread within these populations.
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Affiliation(s)
- Courtenay B. Bombara
- Sydney School of Veterinary Science, The University of Sydney, Camden, Australia
| | - Salome Dürr
- Veterinary Public Health Institute, University of Bern, Liebefeld, Switzerland
| | - Gabriel E. Machovsky-Capuska
- Sydney School of Veterinary Science, The University of Sydney, Camden, Australia
- The Charles Perkins Centre and School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia
| | - Peter W. Jones
- School of Electrical and Information Engineering, The University of Sydney, Sydney, Australia
| | - Michael P. Ward
- Sydney School of Veterinary Science, The University of Sydney, Camden, Australia
- * E-mail:
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13
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Romig T, Deplazes P, Jenkins D, Giraudoux P, Massolo A, Craig PS, Wassermann M, Takahashi K, de la Rue M. Ecology and Life Cycle Patterns of Echinococcus Species. ADVANCES IN PARASITOLOGY 2017; 95:213-314. [PMID: 28131364 DOI: 10.1016/bs.apar.2016.11.002] [Citation(s) in RCA: 295] [Impact Index Per Article: 36.9] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Subscribe] [Scholar Register] [Indexed: 01/31/2023]
Abstract
The genus Echinococcus is composed of eight generally recognized species and one genotypic cluster (Echinococcus canadensis cluster) that may in future be resolved into one to three species. For each species, we review existing information on transmission routes and life cycles in different geographical contexts and - where available - include basic biological information of parasites and hosts (e.g., susceptibility of host species). While some Echinococcus spp. are transmitted in life cycles that involve predominantly domestic animals (e.g., dog - livestock cycles), others are wildlife parasites that do or do not interact with domestic transmission. In many cases, life cycle patterns of the same parasite species differ according to geography. Simple life cycles contrast with transmission patterns that are highly complex, involving multihost systems that may include both domestic and wild mammals. Wildlife transmission may be primary or secondary, i.e., resulting from spillovers from domestic animals. For most of the species and regions, existing information does not yet permit a conclusive description of transmission systems. Such data, however, would be highly relevant, e.g., for anticipation of geographical changes of the presence and frequency of these parasites in a warming world, or for initiating evidence-based control strategies.
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Affiliation(s)
- T Romig
- University of Hohenheim, Stuttgart, Germany
| | - P Deplazes
- University of Zürich, Zurich, Switzerland
| | - D Jenkins
- Charles Sturt University, Wagga Wagga, NSW, Australia
| | - P Giraudoux
- University of Franche-Comté and Institut Universitaire de France, Besancon, France
| | - A Massolo
- University of Calgary, Calgary, Alberta, Canada
| | - P S Craig
- University of Salford, Greater Manchester, United Kingdom
| | | | | | - M de la Rue
- University of Santa Maria, Santa Maria RS, Brazil
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Abstract
Human cystic echinococcosis (CE) has been eliminated or significantly reduced as a public health problem in several previously highly endemic regions. This has been achieved by the long-term application of prevention and control measures primarily targeted to deworming dogs, health education, meat inspection, and effective surveillance in livestock and human populations. Human CE, however, remains a serious neglected zoonotic disease in many resource-poor pastoral regions. The incidence of human alveolar echinococcosis (AE) has increased in continental Europe and is a major public health problem in parts of Eurasia. Better understanding of wildlife ecology for fox and small mammal hosts has enabled targeted anthelmintic baiting of fox populations and development of spatially explicit models to predict population dynamics for key intermediate host species and human AE risk in endemic landscapes. Challenges that remain for echinococcosis control include effective intervention in resource-poor communities, better availability of surveillance tools, optimal application of livestock vaccination, and management and ecology of dog and wildlife host populations.
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Narayan EJ, Williams M. Understanding the dynamics of physiological impacts of environmental stressors on Australian marsupials, focus on the koala (Phascolarctos cinereus). BMC ZOOL 2016. [DOI: 10.1186/s40850-016-0004-8] [Citation(s) in RCA: 12] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/26/2023] Open
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Rahimi M, Sarvi S, Daryani A, Sharif M, Ahmadpour E, Shokri A, Mizani A. Application of multiplex PCR for the simultaneous detection of Taenia spp. from domestic dogs in the north of Iran. Helminthologia 2016. [DOI: 10.1515/helmin-2016-0017] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/15/2022] Open
Abstract
Summary
The family Taeniidae is of great importance in the medical and veterinary fields, particularly in the tropics and subtropics. Identification of eggs of different Taenia spp. in the final host by morphological examination is difficult owing to their similarity. Therefore, a multiplex polymerase chain reaction (PCR) targeting a mitochondrial gene was applied to identify morphologically indistinguishable eggs. Fecal samples from 100 domestic dogs, from the Mazandaran province in Iran, were examined using the flotation/sieving method followed by multiplex PCR. Taeniid eggs were observed in 24 % samples, of which 12 %, 10 %, and 2 % were infected with Echinococcus granulosus, Taenia spp., and both E. granulosus and Taenia spp., respectively. E. multilocularis was absent in these samples. The prevalence of E. granulosus in the examined domestic dogs as definitive hosts in north of Iran was high (14 %). Therefore, people living in this region of Iran are in danger of acquiring hydatid cyst, which is a serious public health problem.
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Affiliation(s)
| | - S. Sarvi
- Toxoplasmosis Research Center, Mazandaran University of Medical Science, Sari, Iran
| | - A. Daryani
- Toxoplasmosis Research Center, Mazandaran University of Medical Science, Sari, Iran
| | - M. Sharif
- Toxoplasmosis Research Center, Mazandaran University of Medical Science, Sari, Iran
| | - E. Ahmadpour
- Infectious and Tropical Diseases Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran
| | - A. Shokri
- Toxoplasmosis Research Center, Mazandaran University of Medical Science, Sari, Iran
| | - A. Mizani
- Toxoplasmosis Research Center, Mazandaran University of Medical Science, Sari, Iran
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Abstract
SUMMARYEchinococcus granulosus (sensu lato) is now recognized as an assemblage of cryptic species, which differ considerably in morphology, development, host specificity (including infectivity/pathogenicity for humans) and other aspects. One of these species, E. granulosus sensu stricto (s.s.), is now clearly identified as the principal agent causing cystic echinococcosis in humans. Previous studies of a small section of the cox1 and nadh1 genes identified two variants of E. granulosus s.s. to be present in Australia; however, no further work has been carried out to characterize the microdiversity of the parasite in its territory. We have analysed the sequence of the full length of the cox1 gene (1609 bp) from 37 isolates of E. granulosus from different hosts and geographic regions of Australia. The analysis shows that seven haplotypes of E. granulosus s.s. not previously described were found, together with five haplotypes known to be present in other parts of the world, including the haplotype EG01 which is widespread and present in all endemic regions. These data extend knowledge related to the geographical spread and host range of E. granulosus s.s. in a country such as Australia in which the parasite established around 200 years ago.
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Cardona GA, Carmena D. A review of the global prevalence, molecular epidemiology and economics of cystic echinococcosis in production animals. Vet Parasitol 2013; 192:10-32. [DOI: 10.1016/j.vetpar.2012.09.027] [Citation(s) in RCA: 146] [Impact Index Per Article: 12.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/23/2012] [Revised: 08/31/2012] [Accepted: 09/18/2012] [Indexed: 11/29/2022]
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Boubaker G, Macchiaroli N, Prada L, Cucher MA, Rosenzvit MC, Ziadinov I, Deplazes P, Saarma U, Babba H, Gottstein B, Spiliotis M. A multiplex PCR for the simultaneous detection and genotyping of the Echinococcus granulosus complex. PLoS Negl Trop Dis 2013; 7:e2017. [PMID: 23350011 PMCID: PMC3547860 DOI: 10.1371/journal.pntd.0002017] [Citation(s) in RCA: 58] [Impact Index Per Article: 4.8] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/18/2012] [Accepted: 12/04/2012] [Indexed: 12/29/2022] Open
Abstract
Echinococcus granulosus is characterized by high intra-specific variability (genotypes G1–G10) and according to the new molecular phylogeny of the genus Echinococcus, the E. granulosus complex has been divided into E. granulosus sensu stricto (G1–G3), E. equinus (G4), E. ortleppi (G5), and E. canadensis (G6–G10). The molecular characterization of E. granulosus isolates is fundamental to understand the spatio-temporal epidemiology of this complex in many endemic areas with the simultaneous occurrence of different Echinococcus species and genotypes. To simplify the genotyping of the E. granulosus complex we developed a single-tube multiplex PCR (mPCR) allowing three levels of discrimination: (i) Echinococcus genus, (ii) E. granulosus complex in common, and (iii) the specific genotype within the E. granulosus complex. The methodology was established with known DNA samples of the different strains/genotypes, confirmed on 42 already genotyped samples (Spain: 22 and Bulgaria: 20) and then successfully applied on 153 unknown samples (Tunisia: 114, Algeria: 26 and Argentina: 13). The sensitivity threshold of the mPCR was found to be 5 ng Echinoccoccus DNA in a mixture of up to 1 µg of foreign DNA and the specificity was 100% when template DNA from closely related members of the genus Taenia was used. Additionally to DNA samples, the mPCR can be carried out directly on boiled hydatid fluid or on alkaline-lysed frozen or fixed protoscoleces, thus avoiding classical DNA extractions. However, when using Echinococcus eggs obtained from fecal samples of infected dogs, the sensitivity of the mPCR was low (<40%). Thus, except for copro analysis, the mPCR described here has a high potential for a worldwide application in large-scale molecular epidemiological studies on the Echinococcus genus. The dog tapeworm Echinococcus granulosus (E. granulosus) is a cosmopolitan parasite. The adult worms reside in the small intestine of their definitive hosts (dogs). Infective eggs are shed with the feces into the environment and are orally ingested by intermediate hosts where they develop into the metacestode (larval) stage, causing cystic echinococcosis (CE) in humans and livestock. Ten intraspecific genotypes of E. granulosus (G1 to G10) have been reported from different intermediate host species. Based on the recently established molecular phylogeny, E. granulosus is now considered a complex consisting of four species: E. granulosus sensu stricto (G1/G2/G3), E. equinus (G4), E. ortleppi (G5) and E. canadensis (G6–G10). Simple and highly discriminative molecular epidemiological approaches are needed to explore dynamics, life cycle patterns, and the pathogenicity of the members of this complex. We here introduce a one-step multiplex PCR (mPCR) protocol for the genotyping and discrimination of the different members of the E. granulosus complex, allowing three levels of discrimination: (i) Echinococcus genus, (ii) E. granulosus complex, and (iii) genetic variants within the E. granulosus complex. The relatively complicated task of E. granulosus complex speciation and genotyping is clearly simplified by mPCR, and this technique therefore represents a useful tool for routine practice.
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Affiliation(s)
- Ghalia Boubaker
- Institute of Parasitology, University of Bern, Bern, Switzerland
- Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland
- University of Monastir, Faculty of Pharmacy, Department of Clinical Biology B, Laboratory of Parasitology and Mycology, Monastir, Tunisia
| | - Natalia Macchiaroli
- Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Buenos Aires, Argentina
| | - Laura Prada
- Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Buenos Aires, Argentina
| | - Marcela A. Cucher
- Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Buenos Aires, Argentina
| | - Mara C. Rosenzvit
- Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Buenos Aires, Argentina
| | - Iskender Ziadinov
- Institute of Parasitology, University of Zurich, Zurich, Switzerland
| | - Peter Deplazes
- Institute of Parasitology, University of Zurich, Zurich, Switzerland
| | - Urmas Saarma
- Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, Tartu, Estonia
| | - Hamouda Babba
- University of Monastir, Faculty of Pharmacy, Department of Clinical Biology B, Laboratory of Parasitology and Mycology, Monastir, Tunisia
| | - Bruno Gottstein
- Institute of Parasitology, University of Bern, Bern, Switzerland
| | - Markus Spiliotis
- Institute of Parasitology, University of Bern, Bern, Switzerland
- * E-mail:
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Kelly EJ, Baldwin TJ, Rood KA. Pathology in practice. Echinococcosis (hydatidosis) in a sheep. J Am Vet Med Assoc 2012; 241:1449-51. [PMID: 23176235 DOI: 10.2460/javma.241.11.1449] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/20/2022]
Affiliation(s)
- E Jane Kelly
- Utah Veterinary Diagnostic Laboratory, College of Agriculture, Utah State University, Logan, UT 84341, USA.
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Banda F, Nalubamba KS, Muma JB, Munyeme M, Mweemba Munang'andu H. A Cross-Sectional Study Investigating Cystic Hydatidosis in Slaughtered Cattle of Western Province in Zambia. ISRN PARASITOLOGY 2012; 2013:468163. [PMID: 27335848 PMCID: PMC4890875 DOI: 10.5402/2013/468163] [Citation(s) in RCA: 11] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 08/09/2012] [Accepted: 09/19/2012] [Indexed: 02/05/2023]
Abstract
A cross-sectional study was conducted from October 2007 to November 2008 to estimate the prevalence of hydatidosis in slaughtered cattle from two abattoirs in Mongu, Western Province, Zambia, using prospective and retrospective data. Out of the 4061 cattle examined during postmortem inspection, 84 (2.1%) were positive for hydatidosis. No cases were detected from Kaoma and Shangombo districts; however, prevalence ranged from 0.6% to 2.5% in districts where it was present. Sex was found to be positively associated with hydatidosis (P = 0.035) with female cattle being more likely to have hydatidosis (OR = 1.62). In the retrospective study (1994 to 2007), annual prevalence of hydatidosis ranged from 1.56% (n = 12,641) in 2006 to 4.7% (n = 2633) in 2001 with an overall prevalence of 3% (4689/158,456). This value is comparable to that observed in cattle slaughtered between October 2007 and November 2008 (2.1%). Hydatidosis was observed in the lungs (51.2%), liver (47.6%), and kidneys (1.2%). The percentage of viable cysts was 43.7%. This study confirms the presence of hydatidosis in cattle in Western Province of Zambia and estimates economic losses due to organ condemnations. Data presented herein provides a useful baseline for developing policy and intervention measures.
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Affiliation(s)
- Fredrick Banda
- Provincial Veterinary Office, Ministry of Livestock and Fisheries, P.O. Box 910034, Mongu 10106, Zambia
| | - King Shimumbo Nalubamba
- Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia
| | - John Bwalya Muma
- Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia
| | - Musso Munyeme
- Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia
| | - Hetron Mweemba Munang'andu
- Section of Aquatic Medicine and Nutrition, Department of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Sciences, Ullevålsveien 72, P.O Box 8146 Dep, 0033 Oslo, Norway
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Barnes T, Deplazes P, Gottstein B, Jenkins D, Mathis A, Siles-Lucas M, Torgerson P, Ziadinov I, Heath D. Challenges for diagnosis and control of cystic hydatid disease. Acta Trop 2012; 123:1-7. [PMID: 22410539 DOI: 10.1016/j.actatropica.2012.02.066] [Citation(s) in RCA: 67] [Impact Index Per Article: 5.2] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/06/2011] [Revised: 02/14/2012] [Accepted: 02/26/2012] [Indexed: 12/12/2022]
Abstract
This paper is based on the experience of the authors, with the aim to define the challenges for Echinococcus granulosus (E.g./CE) diagnosis and control for those countries that may now or in the future be contemplating control of hydatid disease. A variety of methods are available for diagnosis in humans but a universal gold standard is lacking. Diagnosis in definitive hosts can avoid necropsy by the use of methods such as coproantigen detection but test performance is variable between populations. A sylvatic cycle adds challenges in some countries and the epidemiology of the parasite in these hosts is poorly understood. Control by solely administering praziquantel to dogs is not effective in developing countries where the disease is endemic. Additional avenues to pursue include the instigation of participatory planning, use of an existing vaccination for intermediate hosts and development of a vaccine and long-acting anthelmitic implants for definitive hosts. Promoting public acceptance of control of the dog population by humane euthanasia and reduced reproduction is also essential.
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23
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Grosso G, Gruttadauria S, Biondi A, Marventano S, Mistretta A. Worldwide epidemiology of liver hydatidosis including the Mediterranean area. World J Gastroenterol 2012; 18:1425-37. [PMID: 22509074 PMCID: PMC3319938 DOI: 10.3748/wjg.v18.i13.1425] [Citation(s) in RCA: 195] [Impact Index Per Article: 15.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 07/02/2011] [Revised: 09/17/2011] [Accepted: 10/14/2011] [Indexed: 02/06/2023] Open
Abstract
The worldwide incidence and prevalence of cystic echinococcosis have fallen dramatically over the past several decades. Nonetheless, infection with Echinococcus granulosus (E. granulosus) remains a major public health issue in several countries and regions, even in places where it was previously at low levels, as a result of a reduction of control programmes due to economic problems and lack of resources. Geographic distribution differs by country and region depending on the presence in that country of large numbers of nomadic or semi-nomadic sheep and goat flocks that represent the intermediate host of the parasite, and their close contact with the final host, the dog, which mostly provides the transmission of infection to humans. The greatest prevalence of cystic echinococcosis in human and animal hosts is found in countries of the temperate zones, including several parts of Eurasia (the Mediterranean regions, southern and central parts of Russia, central Asia, China), Australia, some parts of America (especially South America) and north and east Africa. Echinococcosis is currently considered an endemic zoonotic disease in the Mediterranean region. The most frequent strain associated with human cystic echinococcosis appears to be the common sheep strain (G1). This strain appears to be widely distributed in all continents. The purpose of this review is to examine the distribution of E. granulosus and the epidemiology of a re-emerging disease such as cystic echinococcosis.
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24
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Abdybekova A, Torgerson P. Frequency distributions of helminths of wolves in Kazakhstan. Vet Parasitol 2012; 184:348-51. [DOI: 10.1016/j.vetpar.2011.09.004] [Citation(s) in RCA: 24] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/18/2011] [Revised: 09/01/2011] [Accepted: 09/06/2011] [Indexed: 11/24/2022]
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25
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Abstract
Hydatid disease (cystic echinococcosis) remains highly prevalent and a serious cause of human morbidity and mortality in many parts of the world. While there are some regions where the disease has been controlled, most efforts to control transmission of the parasite have had limited success. Recent genetic data indicates that Echinococcus granulosus, which was formally thought to be a single species, comprises a number of distinct species. The vast majority of human infections are caused by the most common genotype which is generally transmitted by sheep and goats. Renewed hope for effective control of the parasite’s transmission has followed the development of the EG95 vaccine that can be used to reduce infection levels in livestock animals thereby reducing the reliance of control measures on interventions in dogs.
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26
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Guo ZH, Kubo M, Kudo M, Nibe K, Horii Y, Nonaka N. Growth and genotypes of Echinococcus granulosus found in cattle imported from Australia and fattened in Japan. Parasitol Int 2011; 60:498-502. [DOI: 10.1016/j.parint.2011.09.002] [Citation(s) in RCA: 24] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/30/2011] [Revised: 08/30/2011] [Accepted: 09/05/2011] [Indexed: 11/27/2022]
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27
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Reappraising the theme of breeding systems in Echinococcus: is outcrossing a rare phenomenon? Parasitology 2010; 138:298-302. [PMID: 20880421 DOI: 10.1017/s0031182010001307] [Citation(s) in RCA: 11] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/05/2022]
Abstract
Selfing has been considered the most common mode of reproduction in Echinococcus flatworms. However, population genetic studies on the asexual larval stage involving nuclear co-dominant markers have not always revealed significant heterozygote deficiencies--the expected outcome of a regularly and highly inbred population. In this study, we analysed the genetic structure of Echinococcus granulosus sensu lato populations from Southern Brazil during their adult (sexual) stage using 1 mitochondrial and 1 nuclear marker (cox 1 and mdh, respectively). We show that parasite genetic differentiation is largest among definitive hosts (domestic dogs) from different farms, suggesting that transmission is mostly maintained within a farm. Moreover, we show that heterozygote deficiencies are not significant, and we suggest that outbreeding is the most common mode of reproduction of the parasite in that region.
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Nonga HE, Karimuribo ED. A retrospective survey of hydatidosis in livestock in Arusha, Tanzania, based on abattoir data during 2005-2007. Trop Anim Health Prod 2009; 41:1253-7. [PMID: 19184507 DOI: 10.1007/s11250-009-9308-9] [Citation(s) in RCA: 16] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/04/2008] [Accepted: 01/21/2009] [Indexed: 10/21/2022]
Abstract
A 3-year (2005-2007) retrospective study was carried out to investigate the occurrence of cystic echinococcosis (CE) in cattle and shoats (sheep and goats) slaughtered at Arusha municipal abattoir, Tanzania. A total of 115,186 cattle and 99,401 shoats were slaughtered. Cattle liver, lungs, spleen and heart condemnation rate was 16.35%, 13.04%, 2.09% and 3.06% respectively while 17.63%, 7.63%, 0.38% and 0.04% of shoats' liver, lungs, spleen and heart respectively were condemned. A highly significant (p < 0.001) cystic echinococcosis (CE) infection rate was recorded in shoats (6.02%) than in cattle (4.2%) probably because of differences in grazing patterns. Cattle lungs were more affected by CE (22.5%) than liver (19.7%) while shoats liver were found to be more affected (21%) than the lungs (19.3). A significant (p < 0.001) higher CE infection rate was observed in cattle spleen (15.5%) than shoats (9.7%). There was no significant (p > 0.05) difference between condemnation rates due to hydatidosis during rain and dry seasons probably because of chronicity of the disease. The study have established that CE is prevalent in animals slaughtered at Arusha abattoir and causes higher condemnation rates of edible offals. This merit for more extensive epidemiological investigations to better determine the prevalence, economic impact and public health importance of the disease in the region.
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Affiliation(s)
- H E Nonga
- Department of Veterinary Medicine and Public Health, Sokoine University of Agriculture, P.O. Box 3021, Chuo Kikuu Morogoro, Tanzania.
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Jenkins DJ, Allen L, Goullet M. Encroachment of Echinococcus granulosus into urban areas in eastern Queensland, Australia. Aust Vet J 2008; 86:294-300. [PMID: 18673469 DOI: 10.1111/j.1751-0813.2008.00327.x] [Citation(s) in RCA: 30] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/28/2023]
Abstract
OBJECTIVE To investigate the prevalence of Echinococcus granulosus in wild dogs (dingos and dingo-domestic dog hybrids) living in and around human habitation on Fraser Island and in townships of the Maroochy Shire, on Queensland's Sunshine Coast, Australia. DESIGN Wild dogs were humanely killed on Fraser Island and in the Maroochy Shire because they were deemed a potential danger to the public. Their intestines were collected and the contents examined for intestinal parasites. PROCEDURE Intestines were removed as soon after death as possible, packed in plastic bags and kept frozen until examination. The intestinal contents were washed, sieved and examined microscopically for the presence of helminths, which were identified and counted. RESULTS Intestines from 108 wild dogs, 7 foxes and 18 Fraser Island dingoes were examined. Echinococcus granulosus was only present in the wild dogs from Maroochy Shire (46.3%) with worm burdens of between 30 and 104,000. Other helminths included Spirometra erinacei, Dipylidium caninum, Taenia spp., Ancylostoma caninum and Toxocara canis. Two specimens of a trematode (Haplorchinae sp.) usually found infecting fish and seabirds were recovered from a Fraser Island dingo. CONCLUSION Dingoes on Fraser Island are not infected with E. granulosus and do not pose a hydatid disease public health risk to residents or visitors. However, wild dogs examined from the Maroochy Shire do present a potential hydatid disease public health risk.
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Affiliation(s)
- D J Jenkins
- Australian Hydatid Control & Research Laboratory, School of Animal & Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW 2678, Australia.
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Claridge AW, Hunt R. Evaluating the role of the Dingo as a trophic regulator: Additional practical suggestions. ECOLOGICAL MANAGEMENT & RESTORATION 2008. [DOI: 10.1111/j.1442-8903.2008.00402.x] [Citation(s) in RCA: 18] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/27/2022]
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Infectivity of Echinococcus granulosus protoscolices under different conditions of temperature and humidity. J Helminthol 2008; 82:297-300. [PMID: 18519005 DOI: 10.1017/s0022149x08995055] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/14/2022]
Abstract
The aim of this study was to examine the effect of different temperatures and humidities on the infectivity of Echinococcus granulosus protoscolices. Eighteen dogs (6 groups, n = 3 each) were fed with offal mince harbouring approximately 20,000 protoscolices of E. granulosus of different viabilities. Dogs were infected with E. granulosus protoscolices of: (1) 5% viability at -10 degrees C and 50% relative humidity (RH); (2) 30% viability at 0 degrees C and 60% RH; (3) 20% viability at +10 degrees C and 65% RH; (4) 15% viability at +30 degrees C and 75% RH; (5) 11% viability at +40 degrees C and 80% RH; (6) 68% viability (control group). Dogs in each group were necropsied at 29-49 days post-infection. Mean intensities of E. granulosus recovered from dogs were 256.7 +/- 60.3 in the second group; 32.7 +/- 7.1 in the third group; 40.3 +/- 15.5 in the fourth group and 1533 +/- 513 in the control group. However, no parasites were recovered from the first and fifth groups. Results obtained in the present study show that larval stages could be infective for 1 to 4 weeks during spring, autumn or winter months when maximal temperatures are approximately 0-10 degrees C. In conclusion, cold-storage depots in slaughterhouses and abattoirs where sheep carcasses might be discarded should be kept at -20 degrees C for 2-3 days, dogs should be properly controlled and adequate control programmes must be established in areas where the disease is endemic.
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Craig PS, Larrieu E. Control of cystic echinococcosis/hydatidosis: 1863-2002. ADVANCES IN PARASITOLOGY 2006; 61:443-508. [PMID: 16735171 DOI: 10.1016/s0065-308x(05)61011-1] [Citation(s) in RCA: 151] [Impact Index Per Article: 7.9] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/12/2022]
Abstract
Echinococcosis/hydatidosis, caused by Echinococcus granulosus, is a chronic and debilitating zoonotic larval cestode infection in humans, which is principally transmitted between dogs and domestic livestock, particularly sheep. Human hydatid disease occurs in almost all pastoral communities and rangeland areas of the underdeveloped and developed world. Control programmes against hydatidosis have been implemented in several endemic countries, states, provinces, districts or regions to reduce or eliminate cystic echinococcosis (CE) as a public health problem. This review assesses the impact of 13 of the hydatid control programmes implemented, since the first was introduced in Iceland in 1863. Five island-based control programmes (Iceland, New Zealand, Tasmania, Falklands and Cyprus) resulted, over various intervention periods (from <15 to >50 years), in successful control of transmission as evidenced by major reduction in incidence rates of human CE, and prevalence levels in sheep and dogs. By 2002, two countries, Iceland and New Zealand, and one island-state, Tasmania, had already declared that hydatid disease had been eliminated from their territories. Other hydatid programmes implemented in South America (Argentina, Chile, Uruguay), in Europe (mid-Wales, Sardinia) and in East Africa (northwest Kenya), showed varying degrees of success, but some were considered as having failed. Reasons for the eventual success of certain hydatid control programmes and the problems encountered in others are analysed and discussed, and recommendations for likely optimal approaches considered. The application of new control tools, including use of a hydatid vaccine, are also considered.
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Affiliation(s)
- P S Craig
- Cestode Zoonoses Research Group, Biomedical Sciences Institute & School of Environment and Life Sciences, University of Salford, Salford, Greater Manchester M5 4WT, UK
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Abstract
OBJECTIVE To provide information on possible ecological determinants of infection with Echinococcus granulosus in a beef pastoral area of northern Queensland. PROCEDURE An ecological study was carried out on the prevalence and viability of infection with Echinococcus granulosus in definitive and potential intermediate hosts, and their predator prey relationships. Seven adjacent extensive beef properties 100 km south of Townsville, that included areas of savannah, open woodland and dense closed scrub, were selected for the study. Infection with E granulosus in dingoes was determined at post mortem, and in domestic dogs by examining duodenal mucus after purging with arecoline hydrobromide. Cattle, wild pigs and macropods were examined at post mortem for viable hydatid cysts. The diet of dingoes was investigated by identifying the hair of prey species found in their stomach and colon, and that of domestic dogs by questioning their owners. RESULTS Prevalence of hydatidosis in adult cattle ranged from 41% in animals from properties with large areas of dense closed scrub, to 3% on properties with little or no scrub. Hydatid cysts were found in 21.8% of black-striped wallabies (Macropus dorsalis), 9.4% of feral pigs, 1.5% of wallaroos (Macropus robustus), and 1.4% of eastern grey kangaroos (Macropus giganteus). No rufous rat kangaroos (Aepyprymnus rufescens) or swamp wallabies (Wallabia bicolor) were infected. Most cysts in macropods were viable, whereas in pigs about half were viable and in cattle only 0.7% contained viable protoscoleces. Infection with E granulosus was detected in 76% of dingoes, whereas no infection was detected in domestic dogs in the study area. CONCLUSIONS It was concluded that the sylvatic cycle of E granulosus in the study area was maintained mainly through predation of black-striped wallabies by dingoes, and that the verges of dense scrub were the main nidus of infection.
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Affiliation(s)
- D J D Banks
- School of Veterinary and Biomedical Sciences, James Cook University, Townsville, Queensland 4811, Australia
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Jenkins DJ, Romig T, Thompson RCA. Emergence/re-emergence of Echinococcus spp.--a global update. Int J Parasitol 2006; 35:1205-19. [PMID: 16157340 DOI: 10.1016/j.ijpara.2005.07.014] [Citation(s) in RCA: 244] [Impact Index Per Article: 12.8] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/05/2005] [Revised: 07/28/2005] [Accepted: 07/28/2005] [Indexed: 12/28/2022]
Abstract
This review provides an update of the biological aspects of the genus Echinococcus and focuses on newly recognized endemic areas. Infection with the intermediate cystic stage of all species of Echinococcus causes disease and incapacity in animals and humans, and in the most serious cases, death of the host. Transmission of Echinococcus to new continents has occurred during European colonisation and the parasite has often taken advantage of Echinococcus-naive wildlife populations in these new environments, incorporating them into its transmission pattern. Echinococcus granulosus consists of a complex of 10 strains. Host specificities of these strains have important implications for transmission and control. As a result of human behaviour and/or political instability in a number of countries Echinococcus is re-emerging as an important public health issue. The importance of wildlife reservoirs in perpetuating transmission and as a source of infection for domestic animals and humans is addressed. The review also refers to the transmission pattern of a recently described new species, Echinococcus shiquicus, from China.
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Affiliation(s)
- D J Jenkins
- Australian Hydatid Control and Epidemiology Program, 12 Mildura Street, Fyshwick, ACT 2609, Australia.
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Heath D, Yang W, Li T, Xiao Y, Chen X, Huang Y, Yang Y, Wang Q, Qiu J. Control of hydatidosis. Parasitol Int 2006; 55 Suppl:S247-52. [PMID: 16387527 DOI: 10.1016/j.parint.2005.11.052] [Citation(s) in RCA: 31] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/17/2022]
Abstract
Control of hydatidosis is less effective without the support of dog-owners, and this support can only be obtained if the people have a clear understanding of the life-cycle of the hydatid parasite(s) and what risk factors contribute to human infections. Dissemination of this information is the biggest challenge for hydatid control. Participatory planning between dog-owners and community leaders should evaluate the possible control technologies, and should enable a choice of those aspects that suit the sociology and economic status of the particular community. Collection of baseline data is essential, as is on-going surveillance. Hydatid control should be mainly self-funded, which again requires the support of the dog-owner. A pilot hydatid control program for Tibetan herdsmen is described.
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Affiliation(s)
- David Heath
- AgResearch, Wallaceville Animal Research Centre, PO Box 40063, Upper Hutt, 6007, New Zealand. ..nz
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Jenkins DJ, McKinlay A, Duolong HE, Bradshaw H, Craig PS. Detection of Echinococcus granulosus coproantigens in faeces from naturally infected rural domestic dogs in south eastern Australia. Aust Vet J 2006; 84:12-6. [PMID: 16498828 DOI: 10.1111/j.1751-0813.2006.tb13116.x] [Citation(s) in RCA: 14] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/13/2022]
Abstract
OBJECTIVE To investigate the occurrence of Echinococcus granulosus in rural domestic dogs in farming areas around Yass, New South Wales, and Mansfield and Whitfield, Victoria. DESIGN Faeces were collected per-rectally from farm dogs voluntarily presented by their owners in four farming districts in New South Wales and two in Victoria. PROCEDURE Faeces were collected in the field, an extract prepared from each sample and E granulosus coproantigens detected in an ELISA. Farmers were also questioned about their dog feeding and worming practices. RESULTS Echinococcus granulosus coproantigens were detected in 99 of 344 dogs (29%) from 95 farms in south eastern New South Wales and 38 of 217 dogs (17.5%) from 43 farms in Victoria. Cross-reactions between E granulosus coproantigen trapping antibody and coproantigens in faeces from dogs monospecifically infected with other species of intestinal helminthes (Taenia ovis, T hydatigena, T pisiformis, Spirometra ericacei, Dipylidium caninum, hookworm, Toxocara canis, Trichuris vulpis) were not evident. Dietary and worming data revealed many owners fed raw meat and occasionally offal from domestic livestock and wildlife to their dogs and few owners wormed their dogs frequently enough to preclude the chance of patent E granulosus being present in their dogs. CONCLUSION Echinococcus granulosus occurs commonly in rural dogs in south eastern Australia and an education program promoting the public health importance of responsible management of rural dogs is urgently needed.
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Affiliation(s)
- D J Jenkins
- Australian Hydatid Control and Epidemiology Program, 12 Mildura Street, Fyshwick, Australian Capital Territory 2609
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Abstract
Echinococcus granulosus is the only member of the Genus Echinococcus to occur in Australia. The major biomass of E. granulosus occurs in wildlife. The wildlife transmission cycle is predominantly perpetuated via a predator/prey interaction between wild dogs (dingoes and dingo/domestic dog hybrids) and macropodid marsupials (wallabies and kangaroos). Other wildlife hosts include foxes, wombats and feral pigs. This wildlife reservoir for E. granulosus "spills over" to help maintain a domestic cycle through E. granulosus-infected wild dogs defecating on pasture, transmitting infection to livestock and some farmers and hunters feeding hydatid-infected offal of macropodids or feral pigs to domestic dogs. The potential transmission risk to humans using public picnic and camping areas in parks and forests, especially in the southeastern Australia, could be substantially reduced through regular distribution of baits containing praziquantel. Encroachment of wild dogs and foxes into urban centers presents a new potential path of transmission from wildlife to humans.
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Affiliation(s)
- David J Jenkins
- Australian Hydatid Control & Epidemiology Program, 12 Mildura Street, Fyshwick, ACT 2609, Australia.
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