Concomitant non-alcoholic fatty liver disease (NAFLD) and coeliac disease (CD) have not been adequately studied. This study investigated the frequency of CD among NAFLD patients and the clinicopathological and immunological patterns and outcome of concomitant NAFLD and CD.

This prospective longitudinal study screened patients with NAFLD for CD (tissue transglutaminase antibodies (TTGA); anti-TTGA and antiendomysial antibodies (EMA)). Patients with concomitant NAFLD and CD and patients with either NAFLD or CD were enrolled and followed. Duodenal biopsy, transient elastography, tumour necrosis factor (TNF)-alpha, transforming growth factor-beta, interleukins (ILs) 1, 6, 10, 15 and 17, folic acid and vitamins B12 and D were performed at baseline and 1 year after gluten-free diet (GFD).

CD was confirmed in 7.2% of patients with NAFLD. Refractory anaemia and nutritional deficiencies were frequent in patients with concomitant NAFLD and CD who had advanced intestinal and hepatic lesions, higher levels of TNF-α, IL-15 and IL-17 compared with patients with CD and NAFLD. Patients concomittant CD and NAFLD showed clinical response to GFD, but intestinal histological improvement was suboptimal. Combining EMA-IgA or anti-TTGA with either IL-15 or IL-17 enhances the prognostic performance of both tests in predicting histological response to GFD.

Concomitant NAFLD and CD is not uncommon. Recurrent abdominal symptoms, refractory anaemia, nutritional deficiencies in patients with NAFLD warrant screening for CD. The study has important clinical implications since failure in diagnosing CD in patients with NAFLD patients results in marked intestinal and hepatic damage and suboptimal response to GFD that can be alleviated by early diagnosis and initiation of GFD.

• celiac disease
• cytokines
• gluten free diet
• nonalcoholic steatohepatitis
• small intestinal biopsy

To investigate the pharmacological effect of TongXie-YaoFang (TXYF) formula, a Chinese herbal formula, on Diarrhea-predominant irritable bowel syndrome (D-IBS) rats.

In a neonatal maternal separation plus restraint stress (NMS + RS) model of D-IBS, male Sprague Dawley rats were randomly divided into two groups (NMS + RS group and TXYF-formula group) with no handlings were used as controls (NH group). Starting from postnatal day 60, rats in TXYF-formula group were administered TXYF-formula (4.92 g/100 g bodyweight) orally twice a day for 14 consecutive days while NH group and NMS + RS group were given distilled water. Using short-circuit current technology, we observed 5-HT-induced changes of current across ion channels, such as cystic fibrosis transmembrane conductance regulator (CFTR) Cl^- channel, epithelial Na⁺ channel (ENaC), Ca²⁺-dependent Cl^- channel (CACC), Na⁺-K⁺-2Cl^- co-transporter (NKCC), and Na⁺-HCO₃^- co-transporter (NBC), in the colonic epithelium of three groups after exposure to drugs and specific blockers with a Power Lab System (AD Instruments International).

Under basal conditions, the changes of short-circuit current (∆Isc, µA/cm²) induced by 5-HT were similar in NH group and TXYF-formula group, and both higher than NMS + RS group (70.86 µA/cm² ± 12.32 µA/cm², 67.67 µA/cm² ± 11.68 µA/cm² vs 38.8 µA/cm² ± 7.25 µA/cm², P < 0.01, respectively). When CACC was blocked by 4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid, 5-HT-induced ∆Isc was smaller in NMS + RS group than in NH group and TXYF-formula group, respectively (48.41 µA/cm² ± 13.15 µA/cm² vs 74.62 µA/cm² ± 10.73 µA/cm², 69.22 µA/cm² ± 11.7 µA/cm², P < 0.05, respectively). The similar result could be obtained when ENaC was blocked by Amiloride (44.69 µA/cm² ± 12.58 µA/cm² vs 62.05 µA/cm² ± 11.26 µA/cm², 62.11 µA/cm² ± 12.01 µA/cm², P < 0.05, respectively). However, when CFTR Cl^- channel was blocked by 1,1-dimethyl piperidinium chloride (DPC), 5-HT-induced ∆Isc did not significantly differ in three groups (42.28 µA/cm² ± 10.61 µA/cm² vs 51.48 µA/cm² ± 6.56 µA/cm² vs 47.75 µA/cm² ± 7.99 µA/cm², P > 0.05, respectively). The similar results could also be obtained in three groups when NBC and NKCC were respectively blocked by their blockers.

TXYF-formula can regulate the Cl^- and HCO₃^- secretion of colonic mucosa via CFTR Cl^- channel, Cl^-/HCO₃^- exchanger, NBC and NKCC co-transporters.

• Ion channel
• Diarrhea-predominant irritable bowel syndrome
• Colonic mucosa
• Short-circuit current
• TongXie-YaoFang formula

• 5-Hydroxytryptophan/pharmacology
• Adult
• Amiloride/pharmacology
• Animals
• Chloride Channels/antagonists & inhibitors
• Chloride Channels/drug effects
• Colon/metabolism
• Diarrhea/drug therapy
• Diarrhea/etiology
• Diarrhea/metabolism
• Drugs, Chinese Herbal/pharmacology
• Epithelial Sodium Channel Blockers/pharmacology
• Humans
• Intestinal Mucosa/drug effects
• Intestinal Mucosa/metabolism
• Irritable Bowel Syndrome/drug therapy
• Irritable Bowel Syndrome/etiology
• Irritable Bowel Syndrome/metabolism
• Male
• Maternal Deprivation
• Piperidines/pharmacology
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Sodium Potassium Chloride Symporter Inhibitors/pharmacology
• Sodium-Bicarbonate Symporters/antagonists & inhibitors
• Sodium-Bicarbonate Symporters/drug effects
• Sodium-Potassium-Chloride Symporters/drug effects
• Stress, Psychological/complications
• Young Adult