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Qian XH, Yan YC, Gao BQ, Wang WL. Prevalence, diagnosis, and treatment of primary hepatic gastrointestinal stromal tumors. World J Gastroenterol 2020; 26:6195-6206. [PMID: 33177793 PMCID: PMC7596635 DOI: 10.3748/wjg.v26.i40.6195] [Citation(s) in RCA: 12] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 05/13/2020] [Revised: 05/27/2020] [Accepted: 09/22/2020] [Indexed: 02/06/2023] Open
Abstract
Gastrointestinal stromal tumors (GIST), which is the most common mesenchymal tumor of the digestive tract, account for 1%-3% of gastrointestinal tumors. Primary stromal tumors outside the gastrointestinal tract are collectively referred to as extra GISTs, and stromal tumors in different regions often have different prognoses. A primary hepatic GIST is a rare tumor with an unknown origin, which may be related to interstitial Cajal-like cells. Although primary hepatic GIST has certain characteristics on imaging, it lacks specific symptoms and signs; thus, the final diagnosis depends on pathological and genetic evidence. This review summarizes all cases of primary hepatic GIST described in the literature and comprehensively analyzes the detailed clinical data of all patients. In terms of treatment, local resection alone or with adjuvant therapy was the prioritized choice to obtain better disease-free survival and longer survival time. For advanced unresectable cases, imatinib mesylate was applied as the first-line chemotherapy agent. Moreover, transcatheter arterial chemoembolization, radiofrequency ablation, and microwave ablation were shown to improve overall survival for selected patients. Liver transplantation was a final treatment option after resistance to chemotherapy developed.
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Affiliation(s)
- Xiao-Hui Qian
- Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, Zhejiang Province, China
- Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of Zhejiang Province, Hangzhou 310009, Zhejiang Province, China
- Research Center of Diagnosis and Treatment Technology for Hepatocellular Carcinoma of Zhejiang Province, Hangzhou 310009, Zhejiang Province, China
- Clinical Medicine Innovation Center of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Disease of Zhejiang University, Hangzhou 310009, Zhejiang Province, China
- Clinical Research Center of Hepatobiliary and Pancreatic Diseases of Zhejiang Province, Hangzhou 310009, Zhejiang Province, China
| | - Ying-Cai Yan
- Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, Zhejiang Province, China
| | - Bing-Qiang Gao
- Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, Zhejiang Province, China
- Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of Zhejiang Province, Hangzhou 310009, Zhejiang Province, China
- Research Center of Diagnosis and Treatment Technology for Hepatocellular Carcinoma of Zhejiang Province, Hangzhou 310009, Zhejiang Province, China
- Clinical Medicine Innovation Center of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Disease of Zhejiang University, Hangzhou 310009, Zhejiang Province, China
- Clinical Research Center of Hepatobiliary and Pancreatic Diseases of Zhejiang Province, Hangzhou 310009, Zhejiang Province, China
| | - Wei-Lin Wang
- Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, Zhejiang Province, China
- Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of Zhejiang Province, Hangzhou 310009, Zhejiang Province, China
- Research Center of Diagnosis and Treatment Technology for Hepatocellular Carcinoma of Zhejiang Province, Hangzhou 310009, Zhejiang Province, China
- Clinical Medicine Innovation Center of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Disease of Zhejiang University, Hangzhou 310009, Zhejiang Province, China
- Clinical Research Center of Hepatobiliary and Pancreatic Diseases of Zhejiang Province, Hangzhou 310009, Zhejiang Province, China
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Höving AL, Schmidt KE, Merten M, Hamidi J, Rott AK, Faust I, Greiner JFW, Gummert J, Kaltschmidt B, Kaltschmidt C, Knabbe C. Blood Serum Stimulates p38-Mediated Proliferation and Changes in Global Gene Expression of Adult Human Cardiac Stem Cells. Cells 2020; 9:cells9061472. [PMID: 32560212 PMCID: PMC7349155 DOI: 10.3390/cells9061472] [Citation(s) in RCA: 13] [Impact Index Per Article: 2.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/15/2020] [Revised: 06/05/2020] [Accepted: 06/13/2020] [Indexed: 12/18/2022] Open
Abstract
During aging, senescent cells accumulate in various tissues accompanied by decreased regenerative capacities of quiescent stem cells, resulting in deteriorated organ function and overall degeneration. In this regard, the adult human heart with a generally low regenerative potential is of extreme interest as a target for rejuvenating strategies with blood borne factors that might be able to activate endogenous stem cell populations. Here, we investigated for the first time the effects of human blood plasma and serum on adult human cardiac stem cells (hCSCs) and showed significantly increased proliferation capacities and metabolism accompanied by a significant decrease of senescent cells, demonstrating a beneficial serum-mediated effect that seemed to be independent of age and sex. However, RNA-seq analysis of serum-treated hCSCs revealed profound effects on gene expression depending on the age and sex of the plasma donor. We further successfully identified key pathways that are affected by serum treatment with p38-MAPK playing a regulatory role in protection from senescence and in the promotion of proliferation in a serum-dependent manner. Inhibition of p38-MAPK resulted in a decline of these serum-mediated beneficial effects on hCSCs in terms of decreased proliferation and accelerated senescence. In summary, we provide new insights in the regulatory networks behind serum-mediated protective effects on adult human cardiac stem cells.
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Affiliation(s)
- Anna L. Höving
- Department of Cell Biology, University of Bielefeld, 33615 Bielefeld, Germany; (K.E.S.); (J.H.); (A.-K.R.); (J.F.W.G.)
- Institute for Laboratory- and Transfusion Medicine, Heart and Diabetes Centre NRW, Ruhr University Bochum, 32545 Bad Oeynhausen, Germany; (I.F.); (C.K.)
- Correspondence: (A.L.H.); (C.K.)
| | - Kazuko E. Schmidt
- Department of Cell Biology, University of Bielefeld, 33615 Bielefeld, Germany; (K.E.S.); (J.H.); (A.-K.R.); (J.F.W.G.)
- Institute for Laboratory- and Transfusion Medicine, Heart and Diabetes Centre NRW, Ruhr University Bochum, 32545 Bad Oeynhausen, Germany; (I.F.); (C.K.)
| | - Madlen Merten
- AG Molecular Neurobiology, University of Bielefeld, 33615 Bielefeld, Germany; (M.M.); (B.K.)
| | - Jassin Hamidi
- Department of Cell Biology, University of Bielefeld, 33615 Bielefeld, Germany; (K.E.S.); (J.H.); (A.-K.R.); (J.F.W.G.)
| | - Ann-Katrin Rott
- Department of Cell Biology, University of Bielefeld, 33615 Bielefeld, Germany; (K.E.S.); (J.H.); (A.-K.R.); (J.F.W.G.)
| | - Isabel Faust
- Institute for Laboratory- and Transfusion Medicine, Heart and Diabetes Centre NRW, Ruhr University Bochum, 32545 Bad Oeynhausen, Germany; (I.F.); (C.K.)
| | - Johannes F. W. Greiner
- Department of Cell Biology, University of Bielefeld, 33615 Bielefeld, Germany; (K.E.S.); (J.H.); (A.-K.R.); (J.F.W.G.)
| | - Jan Gummert
- Department of Thoracic and Cardiovascular surgery, Heart and Diabetes Centre NRW, Ruhr-University Bochum, 32545 Bad Oeynhausen, Germany;
| | - Barbara Kaltschmidt
- AG Molecular Neurobiology, University of Bielefeld, 33615 Bielefeld, Germany; (M.M.); (B.K.)
| | - Christian Kaltschmidt
- Department of Cell Biology, University of Bielefeld, 33615 Bielefeld, Germany; (K.E.S.); (J.H.); (A.-K.R.); (J.F.W.G.)
- Correspondence: (A.L.H.); (C.K.)
| | - Cornelius Knabbe
- Institute for Laboratory- and Transfusion Medicine, Heart and Diabetes Centre NRW, Ruhr University Bochum, 32545 Bad Oeynhausen, Germany; (I.F.); (C.K.)
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Rusu MC, Hostiuc S, Fildan AP, Tofolean DE. Critical Review: What Cell Types Are the Lung Telocytes? Anat Rec (Hoboken) 2019; 303:1280-1292. [PMID: 31443120 DOI: 10.1002/ar.24237] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/21/2018] [Revised: 03/11/2019] [Accepted: 05/13/2019] [Indexed: 12/15/2022]
Abstract
Telocytes (TCs) are stromal cells defined by peculiar long, thin, moniliform prolongations known as telopodes. When isolated, their morphology often lacks the specificity for the proper definition of a particular cell type. Recent studies have linked TCs with different functions and different cell lineages. Although some authors have studied pulmonary TCs, their research has important limitations that we will attempt to summarize in this article. We will focus our analysis on the following: the culture methods used to study them, the lack of proper discrimination of TCs from lymphatic endothelial cells (LECs), whose ultrastructures are very similar, and the immune phenotype of TCs, which may appear in other cell types such as those related to the endothelial lineage or stem/progenitor cells. In conclusion, the cellular diagnosis of lung TCs should be considered with caution until properly designed studies can positively identify these cells and differentiate them from other cell types such as LECs and stem/progenitor cells. Anat Rec, 303:1280-1292, 2020. © 2019 American Association for Anatomy.
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Affiliation(s)
- Mugurel C Rusu
- Division of Anatomy, Faculty of Dental Medicine, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania
| | - Sorin Hostiuc
- Department of Legal Medicine and Bioethics, Faculty of Dental Medicine, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania
| | - Ariadna P Fildan
- Internal Medicine Department, Faculty of Medicine, Ovidius University of Constanţa, Constatnţa, Romania
| | - Doina E Tofolean
- Internal Medicine Department, Faculty of Medicine, Ovidius University of Constanţa, Constatnţa, Romania
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Petrea C, Crăiţoiu Ş, Vrapciu A, Mănoiu V, Rusu M. The telopode- and filopode-projecting heterogeneous stromal cells of the human sclera niche. Ann Anat 2018; 218:129-140. [DOI: 10.1016/j.aanat.2017.12.013] [Citation(s) in RCA: 14] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/27/2017] [Revised: 12/13/2017] [Accepted: 12/15/2017] [Indexed: 12/23/2022]
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Dobra MA, Vrapciu AD, Pop F, Petre N, Rusu MC. The molecular phenotypes of ureteral telocytes are layer-specific. Acta Histochem 2018; 120:41-45. [PMID: 29153593 DOI: 10.1016/j.acthis.2017.11.003] [Citation(s) in RCA: 16] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/10/2017] [Revised: 11/12/2017] [Accepted: 11/13/2017] [Indexed: 01/16/2023]
Abstract
Telocytes (TC) are the delicate interstitial (stromal) cells defined by their long, thin and moniliform processes termed telopodes. Numerous studies determined that different subsets of telocytes populate almost all tissues and attempted to relate these subsets to various functions, from cell signaling to tissue repair and regeneration. Extremely few studies addressed the urinary tract though few data on the molecular pattern of the urinary TCs actually exist. We therefore hypothesized that subsets of urinary TCs co-localize within the human ureter and we aimed at performing an immunohistochemical study to evaluate the tissue-specific molecular pattern of TCs. On sample tissues of proximal ureter drawn from ten human adult patients during surgery were applied primary antibodies against CD34, CD105, von Willebrand Factor, the heavy chain of smooth muscle myosin (SMM) and c-erbB-2. The molecular pattern indicated three different subsets of ureteral TCs which are neither endothelial nor epithelial in nature: (a) type I: the CD34-/CD105+ TCs of the superficial layer of lamina propria; (b) type II: the CD34+/CD105± myoid TCs of the deep layer of lamina propria and (c) type III: the CD34+/CD105+ perivascular TCs. Although apparently different, all these subsets of TCs could belong to the stem/progenitor niche of the ureter.
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Affiliation(s)
- M A Dobra
- Division of Anatomy, Faculty of Dental Medicine, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania
| | - A D Vrapciu
- Division of Anatomy, Faculty of Dental Medicine, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania
| | - F Pop
- MEDCENTER - Center of Excellence in Laboratory Medicine and Pathology
| | - N Petre
- "Carol Davila" Clinical Hospital of Nephrology, Bucharest, Romania
| | - M C Rusu
- Division of Anatomy, Faculty of Dental Medicine, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania; MEDCENTER - Center of Excellence in Laboratory Medicine and Pathology.
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