|
1
|
Lechien JR, Ragrag K, Kasongo J, Favier V, Mayo-Yanez M, Chiesa-Estomba CM, Iannella G, Cammaroto G, Saibene AM, Vaira LA, Carsuzaa F, Sagandykova K, Fieux M, Lisan Q, Hans S, Maniaci A. Association between Helicobacter pylori, reflux and chronic rhinosinusitis: a systematic review. Eur Arch Otorhinolaryngol 2025:10.1007/s00405-025-09212-3. [PMID: 39893593 DOI: 10.1007/s00405-025-09212-3] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/28/2024] [Accepted: 01/07/2025] [Indexed: 02/04/2025]
Abstract
BACKGROUND The prevalence, role, and clinical relevance of Helicobacter Pylori (HP) in sinonasal tissues of patients with chronic rhinosinusitis remain unclear. OBJECTIVE To investigate the prevalence and clinical relevance of Helicobacter Pylori (HP) in chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) and without nasal polyps (CRSSNP). METHODS Three investigators conducted a PubMed, Scopus, and Cochrane Library systematic review of the prevalence and clinical relevance of HP infection in CRS patients through the PRISMA framework. A bias analysis was conducted to identify potential heterogeneity and biases across studies. RESULTS Of the 42 identified studies, 20 met the inclusion criteria, accounting for 741 CRS patients and 368 controls. HP was detected in 37.1% (n = 127/342) of polyps of CRSwNP patients with the polymerase chain reaction (PCR) and 32.7% (n = 37/113) of polyp tissue with the immunohistochemistry (IHC). Controls reported a nasal PCR and IHC detection rates of 14.8% (n = 36/243) and 3.6% (n = 3/84), respectively. The HP rate did not differ between CRSwNP and CRSsNP. Among patients with CRS, the enzyme-linked immunosorbent assay testing detected blood HP antigens in 48.7% (n = 74/152) of CRS patients and 41.6% (n = 37/89) of controls. The detection of HP in polyps was associated with the severity of gastroesophageal reflux disease (GERD). There was an important heterogeneity between studies for the inclusion criteria, methods of HP detection, and reflux outcomes. CONCLUSION Helicobacter Pylori can be detected in one-third of sinonasal tissues from patients with CRS and can be considered a biomarker of GERD. The potential role of HP in the development of CRS remains unclear. The heterogeneity between studies limits the drawing of valid conclusions.
Collapse
Affiliation(s)
- Jerome R Lechien
- Department of Surgery, UMONS Research Institute for Health Sciences and Technology, University of Mons (UMons), Mons, Belgium.
- Department of Otolaryngology-Head Neck Surgery, Foch Hospital, School of Medicine, UFR Simone Veil, Université Versailles Saint-Quentin-en-Yvelines (Paris Saclay University), Paris, France.
- Research Committee, Young Otolaryngologists of the International Federation of Otorhinolaryngological Societies, Paris, France.
- Department of Surgery, Faculty of Medicine, UMONS Research Institute for Health Sciences and Technology, Avenue du Champ de Mars, 6, B7000, Mons, Belgium.
| | - Kamal Ragrag
- Department of Surgery, UMONS Research Institute for Health Sciences and Technology, University of Mons (UMons), Mons, Belgium
| | - Jason Kasongo
- Department of Surgery, UMONS Research Institute for Health Sciences and Technology, University of Mons (UMons), Mons, Belgium
| | - Valentin Favier
- Research Committee, Young Otolaryngologists of the International Federation of Otorhinolaryngological Societies, Paris, France
- Department of Otolaryngology-Head and Neck Surgery, CHU de Montpellier, Montpellier, France
| | - Miguel Mayo-Yanez
- Research Committee, Young Otolaryngologists of the International Federation of Otorhinolaryngological Societies, Paris, France
- Otorhinolaryngology-Head and Neck Surgery Department, Complexo Hospitalario Universitario A Coruña (CHUAC), A Coruña, Galicia, Spain
| | - Carlos M Chiesa-Estomba
- Research Committee, Young Otolaryngologists of the International Federation of Otorhinolaryngological Societies, Paris, France
- Department of Otorhinolaryngology-Head & Neck Surgery, Hospital Universitario Donostia, San Sebastian, Spain
| | - Giannicola Iannella
- Research Committee, Young Otolaryngologists of the International Federation of Otorhinolaryngological Societies, Paris, France
- Organi di Senso Department, Sapienza University of Rome, Viale del Policlinico 151, 00161, Rome, Italy
| | - Giovanni Cammaroto
- Research Committee, Young Otolaryngologists of the International Federation of Otorhinolaryngological Societies, Paris, France
- Head-Neck, and Oral Surgery Unit, Department of Head-Neck Surgery, Otolaryngology, Morgagni Pierantoni Hospital, 47121, Forlì, Italy
| | - Alberto M Saibene
- Research Committee, Young Otolaryngologists of the International Federation of Otorhinolaryngological Societies, Paris, France
- Otolaryngology Unit, Santi Paolo e Carlo Hospital, Department of Health Sciences, Università degli Studi di Milano, 20121, Milan, Italy
| | - Luigi A Vaira
- Research Committee, Young Otolaryngologists of the International Federation of Otorhinolaryngological Societies, Paris, France
- Maxillofacial Surgery Unit, Department of Medicine, Surgery and Pharmacy, University of Sassari, 07100, Sassari, Italy
- PhD School of Biomedical Science, Biomedical Sciences Department, University of Sassari, Sassari, Italy
| | - Florent Carsuzaa
- Research Committee, Young Otolaryngologists of the International Federation of Otorhinolaryngological Societies, Paris, France
- Department of Otolaryngology-Head and Neck Surgery, CHU de Poitiers, Poitiers, France
| | - Kalamkas Sagandykova
- Research Committee, Young Otolaryngologists of the International Federation of Otorhinolaryngological Societies, Paris, France
- Department of Otorhinolaryngology, NpJSC "Astana Medical University", 010000, Astana, Kazakhstan
| | - Maxime Fieux
- Research Committee, Young Otolaryngologists of the International Federation of Otorhinolaryngological Societies, Paris, France
- Hospices Civils de Lyon, Hôpital Lyon Sud, Service d'ORL, d'otoneurochirurgie et de Chirurgie Cervico-Faciale, 69310, Pierre Bénite, France
- Université de Lyon, Université Lyon 1, 69003, Lyon, France
- UMR 5305, Laboratoire de Biologie Tissulaire et d'Ingénierie Thérapeutique, Institut de Biologie et Chimie des Protéines, CNRS/Université Claude Bernard Lyon 1, 7 Passage du Vercors, CEDEX 07, 69003, Lyon, France
| | - Quentin Lisan
- Department of Otolaryngology-Head Neck Surgery, Foch Hospital, School of Medicine, UFR Simone Veil, Université Versailles Saint-Quentin-en-Yvelines (Paris Saclay University), Paris, France
- Research Committee, Young Otolaryngologists of the International Federation of Otorhinolaryngological Societies, Paris, France
| | - Stephane Hans
- Department of Otolaryngology-Head Neck Surgery, Foch Hospital, School of Medicine, UFR Simone Veil, Université Versailles Saint-Quentin-en-Yvelines (Paris Saclay University), Paris, France
- Research Committee, Young Otolaryngologists of the International Federation of Otorhinolaryngological Societies, Paris, France
| | - Antonino Maniaci
- Research Committee, Young Otolaryngologists of the International Federation of Otorhinolaryngological Societies, Paris, France
- Faculty of Medicine and Surgery, University of Enna Kore, 94100, Enna, Italy
| |
Collapse
|
|
2
|
Doulberis M, Kountouras J, Stadler T, Meerwein C, Polyzos SA, Kulaksiz H, Chapman MH, Rogler G, Riva D, Linas I, Kavaliotis J, Kazakos E, Mouratidou M, Liatsos C, Papaefthymiou A. Association between Helicobacter pylori Infection and Nasal Polyps: A Systematic Review and Meta-Analysis. Microorganisms 2023; 11:1581. [PMID: 37375083 DOI: 10.3390/microorganisms11061581] [Citation(s) in RCA: 5] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/28/2023] [Revised: 05/20/2023] [Accepted: 05/29/2023] [Indexed: 06/29/2023] Open
Abstract
BACKGROUND Helicobacter pylori (H. pylori) has definite or possible associations with multiple local and distant manifestations. H. pylori has been isolated from multiple sites throughout the body, including the nose. Clinical non-randomized studies with H. pylori report discrepant data regarding the association between H. pylori infection and nasal polyps. The aim of this first systematic review and meta-analysis was the assessment of the strength of the association between H. pylori infection and incidence of nasal polyps. METHODS We performed an electronic search in the three major medical databases, namely PubMed, EMBASE and Cochrane, to extract and analyze data as per PRISMA guidelines. RESULTS Out of 57 articles, 12 studies were graded as good quality for analysis. Male-to-female ratio was 2:1, and age ranged between 17-78 years. The cumulative pooled rate of H. pylori infection in the nasal polyp group was 32.3% (controls 17.8%). The comparison between the two groups revealed a more significant incidence of H. pylori infection among the nasal polyp group (OR 4.12), though with high heterogeneity I2 = 66%. Subgroup analysis demonstrated that in European studies, the prevalence of H. pylori infection among the nasal polyp group was significantly higher than in controls, yielding null heterogeneity. Subgroup analysis based on immunohistochemistry resulted in null heterogeneity with preserving a statistically significant difference in H. pylori infection prevalence between the groups. CONCLUSION The present study revealed a positive association between H. pylori infection and nasal polyps.
Collapse
Affiliation(s)
- Michael Doulberis
- Gastroklinik, Private Gastroenterological Practice, 8810 Horgen, Switzerland
- Department of Gastroenterology, University Hospital Zurich, 8091 Zurich, Switzerland
- Division of Gastroenterology and Hepatology, Medical University Department, Kantonsspital Aarau, 5001 Aarau, Switzerland
- Department of Internal Medicine, Second Medical Clinic, Ippokration Hospital, Aristotle University of Thessaloniki, 54642 Thessaloniki, Macedonia, Greece
| | - Jannis Kountouras
- Department of Internal Medicine, Second Medical Clinic, Ippokration Hospital, Aristotle University of Thessaloniki, 54642 Thessaloniki, Macedonia, Greece
| | - Thomas Stadler
- Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Zurich, 8091 Zurich, Switzerland
| | - Christian Meerwein
- Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Zurich, 8091 Zurich, Switzerland
| | - Stergios A Polyzos
- First Laboratory of Pharmacology, School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Macedonia, Greece
| | - Hasan Kulaksiz
- Gastroklinik, Private Gastroenterological Practice, 8810 Horgen, Switzerland
| | - Michael H Chapman
- Pancreaticobiliary Medicine Unit, University College London Hospitals (UCLH), London NW1 2BU, UK
| | - Gerhard Rogler
- Department of Gastroenterology, University Hospital Zurich, 8091 Zurich, Switzerland
| | - Daniele Riva
- Gastrocentro Plus, Private Gastroenterological Practice, 6900 Lugano, Switzerland
| | - Ioannis Linas
- Gastroenterologische Gruppenpraxis, Private Gastroenterological Practice, 3011 Bern, Switzerland
| | - John Kavaliotis
- Department of Internal Medicine, Second Medical Clinic, Ippokration Hospital, Aristotle University of Thessaloniki, 54642 Thessaloniki, Macedonia, Greece
| | - Evangelos Kazakos
- Department of Internal Medicine, Second Medical Clinic, Ippokration Hospital, Aristotle University of Thessaloniki, 54642 Thessaloniki, Macedonia, Greece
- School of Healthcare Sciences, Midwifery Department, University of West Macedonia, 50100 Kozani, Macedonia, Greece
| | - Maria Mouratidou
- Department of Internal Medicine, Second Medical Clinic, Ippokration Hospital, Aristotle University of Thessaloniki, 54642 Thessaloniki, Macedonia, Greece
| | - Christos Liatsos
- Department of Gastroenterology, 401 General Military Hospital of Athens, 11525 Athens, Attica, Greece
| | - Apostolis Papaefthymiou
- Pancreaticobiliary Medicine Unit, University College London Hospitals (UCLH), London NW1 2BU, UK
| |
Collapse
|
|
3
|
Zika G, Fousekis FS, Exarchakos G, Baltayiannis G. Detection of Helicobacter Pylori in Nasal Polyps: An Epidemiological Study. Indian J Otolaryngol Head Neck Surg 2023; 75:1084-1089. [PMID: 37206703 PMCID: PMC10188891 DOI: 10.1007/s12070-023-03585-9] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/09/2022] [Accepted: 02/16/2023] [Indexed: 03/06/2023] Open
Abstract
Many studies have described the detection of Helicobacter pylori (HP) in the nasal polyps; however, although gastroesophageal reflux has been associated with chronic rhinosinusitis and nasal polyps development, the role of HP remains unclear. Our aim was to describe the prevalence of HP detection in nasal polyps and its association with gastric HP infection and gastroesophageal reflux dsease (GERD). The prospective study involved 36 patients with nasal polyps, who underwent to endoscopic surgery removal of nasal polyps. Before surgical procedure all patients were tested for gastric HP infection by 13 C-urea breath test, while tissue samples from nasal polyps were tested for HP detection, using rapid urease test (CLO test) and histological examination with Giemsa stain. All patients were asked about GERD-related symptoms. HP in nasal polyps was detected in 9 out of 36 patients (25%) using histological examination with Giemsa stain, while the detection rate of HP was 30.5% (11/36) using CLO test. Furthermore, 28 out of 36 patients (77.7%) had gastric HP infection. All patients with HP colonization in nasal polyps had gastric HP infection and all patients with HP in nasal polyps reported symptoms related to GERD. HP was detected in approximately one out of three patients in nasal polyps, while all patients with HP detection in nasal polyps had concurrently gastric HP infection and reported GERD-related symptoms, suggesting a gastro-nasal route of HP.
Collapse
Affiliation(s)
- Giolanta Zika
- Department of Gastroenterology, School of Health Sciences, Faculty of Medicine, University Hospital of Ioannina, University of Ioannina, Ioannina, Greece
| | - Fotios S. Fousekis
- Department of Gastroenterology, School of Health Sciences, Faculty of Medicine, University Hospital of Ioannina, University of Ioannina, Ioannina, Greece
| | - Georgios Exarchakos
- Department of Otorhinolaryngology, Head and Neck Surgery, School of Health Sciences, Faculty of Medicine, University Hospital of Ioannina, University of Ioannina, Ioannina, Greece
| | - Gerasimos Baltayiannis
- Department of Gastroenterology and Hepatology, School of Health Sciences, Faculty of Medicine, University Hospital of Ioannina, University of Ioannina, PO Box 1186, 45110 Ioannina, Greece
| |
Collapse
|
|
4
|
Nemati S, Jafarinezhad A, Alavi Foumani A, Kazemnezhad Leili E, Farhadi F, Tangestaninejad A, Akbari M. The effects of functional endoscopic sinus surgery on chronic obstructive pulmonary disease (COPD) and asthma: A comparative study. Am J Otolaryngol 2022; 43:103478. [PMID: 35561429 DOI: 10.1016/j.amjoto.2022.103478] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/06/2022] [Revised: 04/16/2022] [Accepted: 05/01/2022] [Indexed: 11/29/2022]
Abstract
PURPOSE Chronic Rhinosinusitis (CRS) can be accompanied by asthma and, rarely by chronic obstructive pulmonary disease (COPD). Functional endoscopic sinus surgery (FESS) is the most common surgical approach for CRS which also improves asthma symptoms, but little is known about its effects on COPD. This study investigates the effects of FESS on COPD and asthma symptoms in CRS patients referred to a university hospital in northern Iran. MATERIALS AND METHODS In a prospective study, patients with COPD or asthma and CRS who were candidates for FESS underwent endoscopic examination, spirometry, and evaluation of symptoms of CRS, COPD, and asthma before, and 2 and 6 months after FESS. Statistical analyzes were performed using SPSS software version 21 and the level of significance was considered as P < 0.05. RESULTS Eighty-two CRS patients (45 with asthma/ mean age: 49.24 ± 12.75 years and 37 patients with COPD/ mean age: 61.43 ± 6.93 years) enrolled. In asthmatic patients, the mean FEV1 and FVC, and ACT (Asthma Control Test) score increased significantly after FESS (P < 0.001). In COPD cases, spirometry indices decreased, but CAT (COPD Assessment Test) score improved significantly (from 15.70 to 32.11) after FESS (P < 0.001). The mean SNOT score in both groups was significantly reduced. CONCLUSIONS In CRS patients with COPD, FESS improves the condition of SNOT-22 and CAT, although does not increase spirometry parameters. In asthmatic patients, FESS improves pulmonary function and asthma symptoms. So both patient groups (i.e. CRS with COPD and with asthma) will benefit from FESS.
Collapse
Affiliation(s)
- Shadman Nemati
- Otorhinolaryngology Research Center, Department of Otolaryngology and Head and Neck Surgery, School of Medicine, Guilan University of Medical Sciences, Amiralmomenin Hospital, Rasht, Iran
| | - Alireza Jafarinezhad
- Inflammatory Lung Diseases Research Center, School of Medicine, Guilan University of Medical Sciences, Razi Hospital, Rasht, Iran
| | - Ali Alavi Foumani
- Inflammatory Lung Diseases Research Center, School of Medicine, Guilan University of Medical Sciences, Razi Hospital, Rasht, Iran
| | - Ehsan Kazemnezhad Leili
- Otorhinolaryngology Research Center, Department of Biostatistics, School of Nursing and Midwifery, Guilan University of Medical Sciences, Rasht, Iran
| | - Fatemeh Farhadi
- Otorhinolaryngology Research Center, Department of Otolaryngology and Head and Neck Surgery, School of Medicine, Guilan University of Medical Sciences, Amiralmomenin Hospital, Rasht, Iran
| | - Azita Tangestaninejad
- Inflammatory Lung Diseases Research Center, School of Medicine, Guilan University of Medical Sciences, Razi Hospital, Rasht, Iran
| | - Maryam Akbari
- Otorhinolaryngology Research Center, Department of Otolaryngology and Head and Neck Surgery, School of Medicine, Guilan University of Medical Sciences, Amiralmomenin Hospital, Rasht, Iran.
| |
Collapse
|
|
5
|
Brown HJ, Kuhar HN, Plitt MA, Husain I, Batra PS, Tajudeen BA. The Impact of Laryngopharyngeal Reflux on Patient-reported Measures of Chronic Rhinosinusitis. Ann Otol Rhinol Laryngol 2020; 129:886-893. [PMID: 32390460 DOI: 10.1177/0003489420921424] [Citation(s) in RCA: 12] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/18/2022]
Abstract
OBJECTIVE This study explored the impact of laryngopharyngeal reflux (LPR) on quality-of-life outcomes captured by Sino-Nasal Outcome Test (SNOT-22) and Reflux Symptom Index (RSI) in patients with chronic rhinosinusitis (CRS) and patients with symptoms of LPR. METHODS In a retrospective chart review, SNOT-22 and RSI scores were analyzed in patients seen at a tertiary care center with CRS, LPR, or both CRS and LPR. SNOT-22 items were grouped into sleep, nasal, otologic, and emotional symptom subdomains. RESULTS A total of 138 patients (36 with CRS alone, 60 with LPR alone, and 42 with both CRS and LPR) were included. Compared to patients with CRS alone, those with CRS and LPR (CRS+LPR) had higher SNOT-22 total (50.54 ± 19.53 vs 35.31 ± 20.20, P < .001), sleep (19.61 ± 9.31 vs 14.42 ± 10.34, P < .022), nasal (17.38 ± 7.49 vs 11.11 ± 8.52, P < .001), otologic subdomains (9.17 ± 5.07 vs 5.53 ± 5.14, P < .002), and RSI (22.06 ± 9.42 vs 10.75 ± 8.43, P < .003). Patients with LPR alone had higher RSI compared to those with CRS (18.48 ± 9.77 vs 10.75 ± 8.43, P < .037). RSI and SNOT-22 scores were positively correlated irrespective of patient group (R = 0.289, P = .003). CONCLUSION Compared to patients with CRS or LPR alone, those with CRS+LPR demonstrated higher RSI and total and subdomain SNOT-22 scores. Patients with LPR alone had elevated SNOT-22 despite absent endoscopic evidence of sinusitis.
Collapse
Affiliation(s)
| | - Hannah N Kuhar
- Department of Otolaryngology, Ohio State University Medical Center, Columbus, OH, USA
| | - Max A Plitt
- Department of Otorhinolaryngology, Rush University Medical Center, Chicago, IL, USA
| | - Inna Husain
- Department of Otorhinolaryngology, Rush University Medical Center, Chicago, IL, USA
| | - Pete S Batra
- Department of Otorhinolaryngology, Rush University Medical Center, Chicago, IL, USA
| | - Bobby A Tajudeen
- Department of Otorhinolaryngology, Rush University Medical Center, Chicago, IL, USA
| |
Collapse
|
|
6
|
Nemati S, Keihanian F, Saeidinia A, Bakhshaei M. Effect Of Bevacizumab On Growth Of Human Nasal Polyposis In Vitro; An Off-Label Use Of Anti-Angiogenic Agent For Nasal Polyposis Treatment. DRUG DESIGN DEVELOPMENT AND THERAPY 2019; 13:3383-3389. [PMID: 31576112 PMCID: PMC6767760 DOI: 10.2147/dddt.s219724] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 06/19/2019] [Accepted: 09/11/2019] [Indexed: 01/02/2023]
Abstract
Introduction Nasal polyposis (NP) is a frequent problem during adulthood. Treatment of NP is primarily based on drugs, such as oral or topical steroids and in some types, by surgery. Despite of available therapeutic options for NP, recurrence after polypectomy is found. Vascular endothelial growth factor (VEGF) is a known factor involved in NP. Bevacizumab is a monoclonal antibody, which acts against VEGF. Aim Regarding the availability of bevacizumab and its use in ophthalmic off-label application, in this study, we hypothesized that it could be a choice of non-invasive treatment. The researchers aimed at evaluating the use of bevacizumab in vitro on the growth of NP. Materials and methods In this experimental study, the researchers used eight non-allergic NP tissues from patients admitted for polypectomy clinic of Imam Reza Hospital, Mashhad. Tissues were cultured in DMEM medium based on standard protocols in the presence or absence of bevacizumab (10 to 250 μM) then incubated. The mean of the responses was reported. The level of VEGF and MTT test for NP epithelial cell viability were determined for each group. Data were analyzed using the SPSS software. Results The researchers demonstrated that bevacizumab leads to a decrease in the level of VEGF (the most common cause of angiogenesis in NP) in media culture of NP, dose-dependently (P<0.001). The highest mean was related to the 10-μM group and the least mean was related to the 250-μM group. In MTT test after 5 days, it was shown that the percentage of viable epithelial NP cells (due to apoptosis) was decreased dose-dependently and could lead to resolving NP tissue (P<0.001), significantly. Conclusion This study showed that bevacizumab could help decrease the growth of NP tissue dose-dependently in organ culture in vitro by inhibiting VEGF. It seems that bevacizumab could be a good candidate for the treatment of non-allergic NP.
Collapse
Affiliation(s)
- Shadman Nemati
- Rhino-sinus, Ear, and Skull Base Diseases Research Center, Guilan University of Medical Sciences, Rasht, Iran
| | - Faeze Keihanian
- Cardiology Department, Imam Reza & Ghaem Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.,Pharmaceutical Research Division, Booali Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
| | - Amin Saeidinia
- Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.,Pharmaceutical Research Division, Booali Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
| | - Mahdi Bakhshaei
- Faculty of Medicine, Ear, Nose and Throat Department, Mashhad University of Medical Sciences, Mashhad, Iran
| |
Collapse
|
|
7
|
Investigation of antibiotic resistance and the presence of integron genes among ESBL producing Klebsiella isolates. Meta Gene 2019. [DOI: 10.1016/j.mgene.2018.10.008] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/18/2022] Open
|
|
8
|
Asl SF, Pourvahedi M, Mojtahedi A, Shenagari M. Analysis of babA, cagE and cagA Genes in Helicobacter pylori from Upper Gastric Patients in the North of Iran. Infect Disord Drug Targets 2019; 19:274-278. [PMID: 29766826 DOI: 10.2174/1871526518666180515113218] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/12/2017] [Revised: 03/20/2018] [Accepted: 04/10/2018] [Indexed: 06/08/2023]
Abstract
OBJECTIVE Helicobacter pylori is a Gram-negative bacterium which has a serious effect on up to half of the world's population and has been related to different gastric diseases. The goal of this study was to assess the frequency of babA, cagE and cagA genotypes among H. pylori strains isolated from gastric biopsies of endoscopic patients in the north of Iran. METHODS The present study was performed on 90 strains of H. pylori isolated from patients with gastric diseases (Gastric ulcer (GU), Duodenal ulcer (DU), Gastritis (G), Non-ulcer dyspepsia (NUD) and Gastric adenocarcinoma (GC)). DNA was extracted from all isolated strains and PCR method was performed to detect the prevalence of babA2, cagE and cagA genes using specific primers. RESULTS Among 90 samples of H. pylori, babA2, cagE, and cagA genes were detected in 42.2%, 30% and 82.2% of strains respectively. The statistical analysis showed that the prevalence of cagA gene in GU, G, DU, and NUD was significantly higher than other genes. Moreover, cagA, and babA2 genes were significantly more prevalent in GC patients compared to cagE gene. Our isolates exhibited 8 distinct arrangements of virulence patterns. The occurrence of cagA (35.6%) was the most prevalent pattern followed by cagA/babA2 (20%) and cagA/babA2/cagE (14.4%). CONCLUSION In summary, as first report from Guilan province in the north of Iran, we showed significant association between the presence of babA2, cagE, and cagA genes in different types of gastric disorders.
Collapse
Affiliation(s)
- Saba Fakhrieh Asl
- Internal Medicine Department, Poursina Hospital, Guilan University of Medical Sciences, Rasht, Iran
| | - Mehrnaz Pourvahedi
- Genetics Department, Faculty of Basic Sciences, Guilan University, Rasht, Iran
| | - Ali Mojtahedi
- Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
- Microbiology Department, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
| | - Mohammad Shenagari
- Microbiology Department, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
| |
Collapse
|
|
9
|
Asgharzadeh Kangachar S, Mojtahedi A. Evaluation of integrons classes 1–3 in extended spectrum beta-lactamases and multi drug resistant Escherichia coli isolates in the North of Iran. GENE REPORTS 2018. [DOI: 10.1016/j.genrep.2018.07.014] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/17/2023]
|
|
10
|
Intranasal Helicobacter pylori infection in patients with chronic rhinosinusitis with polyposis. The Journal of Laryngology & Otology 2018; 132:816-821. [DOI: 10.1017/s0022215118001299] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/10/2023]
Abstract
AbstractObjectiveTo determine the prevalence of Helicobacter pylori infection in nasal biopsy specimens from patients with chronic rhinosinusitis with polyposis versus control patients, and to assess the correlations between H pylori infection identified in the nasal tissue and patients’ sociodemographic data and reflux-related symptoms and signs.MethodsNasal biopsy samples were taken from 75 adult patients who underwent nasal surgery for chronic rhinosinusitis with polyposis (clinical group, n = 45) and a deviated septum (control group, n = 30). H pylori infection was identified using histochemical and rapid urease tests.ResultsThe prevalence of intranasal H pylori infection was significantly higher in the clinical group (28.9 per cent) compared to the control group (3.3 per cent) (p = 0.005). A significant yet weak association was found between positive H pylori status and laryngopharyngeal reflux related hypertrophy of the posterior commissure of the larynx. No other correlations reached statistical significance.ConclusionH pylori infection is potentially related to chronic rhinosinusitis with polyposis. Further research is needed to clarify the role of H pylori as a risk factor for the development of sinonasal diseases and to examine its link with laryngopharyngeal reflux.
Collapse
|
|
11
|
Faghani M, Keshavars P, Sharafshah A, Pourgholamali B, Moharami F, Nemati S. TNF-alpha G/A308 polymorphism association with nasal polyposis in North part of Iran. Eur Arch Otorhinolaryngol 2018; 275:2253-2257. [PMID: 30003390 DOI: 10.1007/s00405-018-5059-4] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/03/2018] [Accepted: 07/02/2018] [Indexed: 11/28/2022]
Abstract
Nasal Polyposis (NP) is a complex multi-factorial disease; associated with several environmental, genetic and inflammatory factors. TNF-alpha is one of the major pro-inflammatory cytokines involved in NP pathogenesis. Some of the polymorphisms of this gene affect its expression. Therefore, the aim of this study was to evaluate the polymorphism of TNF-alpha G/A308 gene and its association with nasal polyposis in north part of Iran. In this case-control study, 60 patients with NP and 75 healthy individuals referred to Guilan University hospital were evaluated. After DNA extraction, RFLP-PCR was used to determine polymorphism. Chi-square test was used to compare the frequency distribution of genotype and alleles of TNF-alpha gene with NP. The frequency of genotype G/G, A/A and G/A in the NP group was 46.7, 10.0 and 43.3%, and in the control group was 65.3, 5.3 and 29.3%, respectively. There was a statistically significant difference between genotype G/G in two groups (P = 0.006, OR 2.58, CI 1.27-5.23). In addition, the frequency of allele A in patients and controls was 31.7 and 20%, respectively; and this difference was statistically significant (p = 0.02). The findings of this study demonstrated that polymorphism in TNF-alpha gene might be a risk factor for NP in north part of Iran and the minor frequency of G308A allele in the current study is slightly more than other major populations. However, more investigations with high number of population are necessary in future.
Collapse
Affiliation(s)
- Masoumeh Faghani
- Anatomical Sciences Department, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.,Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
| | - Parvaneh Keshavars
- Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
| | - Alireza Sharafshah
- Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
| | - Babak Pourgholamali
- Anatomical Sciences Department, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
| | - Farshad Moharami
- Anatomical Sciences Department, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
| | - Shadman Nemati
- Rino-sinus, Ear, and Skull base Diseases Research Center, Amir-al Momenin University Hospital, Guilan University of Medical Sciences, 17 Shahrivar Ave, Rasht, Guilan, Iran.
| |
Collapse
|
|
12
|
Bansal D, Sharma S, Agarwal S, Saha R, Gupta N. Detection of Helicobacter pylori in Nasal Polyps. Head Neck Pathol 2016; 10:306-13. [PMID: 26830396 PMCID: PMC4972759 DOI: 10.1007/s12105-016-0699-4] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 12/20/2015] [Accepted: 01/25/2016] [Indexed: 12/14/2022]
Abstract
To detect the presence of Helicobacter pylori in nasal polyps. A case-control study was conducted enrolling 35 patients with nasal polyps (cases) and patients undergoing septoplasty (controls). Fresh tissue samples were used for urea broth test and imprint cytology, while formalin fixed tissue sections were used for morphology, special stains and immunohistochemistry for H. pylori. Fresh stool samples from both groups were tested to correlate the gastrointestinal status. H. pylori was detected in 40.0 % (14/35) of cases and 8.5 % of controls (3/35) (p = 0.004) by immunohistochemistry. Amongst cases, eight were positive with urea broth test, six with imprint cytology (Giemsa stain), three with H & E, and nine with modified McMullen's stain. Hyperplasia of the lining epithelium and lymphoid aggregates were significantly noticed in nasal polyps positive for H. pylori. Stool antigen test was positive in subjects who were positive for H. pylori in the nasal mucosa. There appears to be an association between H. pylori and nasal polyps. Immunohistochemistry is more sensitive and specific method to detect H. pylori. H. pylori induced inflammatory tissue reaction pattern indicates a possible causal association. Further studies are needed to prove the causal relationship between H. pylori and nasal polyps.
Collapse
Affiliation(s)
- Divya Bansal
- Department of Pathology, University College of Medical Sciences and GTB Hospital, University of Delhi, Dilshad Garden, Delhi, 110095, India
| | - Sonal Sharma
- Department of Pathology, University College of Medical Sciences and GTB Hospital, University of Delhi, Dilshad Garden, Delhi, 110095, India.
| | - Sarla Agarwal
- Department of Pathology, University College of Medical Sciences and GTB Hospital, University of Delhi, Dilshad Garden, Delhi, 110095, India
| | - Rumpa Saha
- Department of Microbiology, University College of Medical Sciences and GTB Hospital, University of Delhi, Delhi, India
| | - Neelima Gupta
- Department of Otorhinolaryngology, University College of Medical Sciences and GTB Hospital, University of Delhi, Delhi, India
| |
Collapse
|
|
13
|
Bakhtiari S, Alvandi A, Pajavand H, Navabi J, Najafi F, Abiri R. Development and Diagnostic Evaluation of Loop-Mediated Isothermal Amplification Using a New Gene Target for Rapid Detection of Helicobacter pylori. Jundishapur J Microbiol 2016; 9:e28831. [PMID: 27540449 PMCID: PMC4976074 DOI: 10.5812/jjm.28831] [Citation(s) in RCA: 16] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/30/2015] [Revised: 11/18/2015] [Accepted: 11/27/2015] [Indexed: 01/05/2023] Open
Abstract
BACKGROUND Helicobacter pylori cause chronic gastritis and subsequent diseases like gastric and duodenal ulcers and gastric adenocarcinoma. Current methods for detecting H. pylori have several disadvantages and it is of utmost importance to develop a simple, quick, accurate, and cost-effective diagnostic test. OBJECTIVES The aim of this study was to set up and evaluate a diagnostic value of loop- mediated isothermal amplification (LAMP) for detecting H. pylori. PATIENTS AND METHODS The analytical sensitivity values (limit of detection) of LAMP and polymerase chain reaction (PCR) were determined using serial dilutions of H. pylori DNA. Analytical specificity of the methods using new designed primers targeted ureC gene was also determined. RESULTS The detection limits of the LAMP and PCR assay were similar and were 10 fg of pure DNA of H. pylori, which is equal to 6 copy numbers of H. pylori genome. Analytical specificity of the tests was 100% because the tests were positive only with H. pylori DNA. CONCLUSIONS The analytical sensitivity of LAMP and PCR methods, using the designed primers, was 8 times more than any other reported methods. The designed methods are specific and sensitive for detection of H. pylori in different clinical and environmental samples.
Collapse
Affiliation(s)
- Somaye Bakhtiari
- Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IR Iran
| | - Amirhooshang Alvandi
- Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IR Iran
| | - Hamid Pajavand
- Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IR Iran
| | - Jafar Navabi
- Imam Khomeini Hospital, Kermanshah University of Medical Sciences, Kermanshah, IR Iran
| | - Farid Najafi
- Department of Biostatistics and Epidemiology, School of Hygiene, Kermanshah University of Medical Sciences, Kermanshah, IR Iran
| | - Ramin Abiri
- Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IR Iran
- Corresponding author: Ramin Abiri, Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, P. O. Box: 6714869914, Kermanshah, IR Iran. Tel: +98-9122773648, Fax: +98-8314274623, E-mail:
| |
Collapse
|
|
14
|
Xin J, Sun H, Kong H, Li L, Zheng J, Yin C, Cao Y, Jia Y, Li C. Intercellular adhesion molecule-1 expression in activated eosinophils is associated with mucosal remodeling in nasal polyps. Mol Med Rep 2015; 11:3391-7. [PMID: 25573100 PMCID: PMC4368088 DOI: 10.3892/mmr.2015.3174] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/07/2014] [Accepted: 12/02/2014] [Indexed: 12/23/2022] Open
Abstract
Nasal polyposis (NP) is characterized by chronic mucosal inflammation with infiltrating eosinophils. Eosinophil-mediated tissue remodeling may be involved in NP pathogenesis; therefore, improved understanding of tissue remodeling may result the identification of novel pathways and therapeutic strategies. The present study aimed to investigate the pathological changes occurring during tissue remodeling in NP, in order to assess the role of intercellular adhesion molecule-1 (ICAM-1) in localized tissue remodeling and the potential association between ICAM-1 expression and markers of eosinophil activation. A total of 28 eligible patients and 10 healthy controls participated in the current study. Nasal mucosal tissues of these subjects were retrospectively evaluated for mucosal remodeling using histopathological staining. ICAM-1 and eosinophil cationic protein (ECP) expression levels were determined by immunohistochemical analysis. Compared with the healthy controls, all the specimens from NP patients presented substantial epithelial damage, skewed cellular distribution with a reduced density of goblet cells, an increased density of subepithelial gland and increased subepithelial collagen deposition. In addition, the NP specimens exhibited significantly higher eosinophil infiltration and ICAM-1 expression compared with the controls. Positive correlations were observed between ICAM-1 and ECP expression levels (P=0.010), as well as between extracellular collagen deposition and ICAM-1 (P=0.010) and ECP (P=0.012) expression levels in the NP specimens, but not in the control specimens. Morphological evidence demonstrated eosinophil-mediated tissue remodeling in NP tissues. ICAM-1 expression in activated eosinophils was associated with NP remodeling, indicating the possibility that ICAM-1 may regulate NP remodeling.
Collapse
Affiliation(s)
- Jingwei Xin
- Department of Otorhinolaryngology‑Head and Neck Surgery, China‑Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China
| | - Hui Sun
- Department of Thyroid Surgery, Jilin Provincial Key Laboratory of Surgical Translational Medicine, China‑Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China
| | - Hong Kong
- Department of Otorhinolaryngology‑Head and Neck Surgery, China‑Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China
| | - Lin Li
- Department of Otorhinolaryngology‑Head and Neck Surgery, China‑Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China
| | - Jun Zheng
- Department of Otorhinolaryngology‑Head and Neck Surgery, China‑Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China
| | - Chunxia Yin
- Department of Gynecology and Obstertrics, Changchun Obstetrics‑Gynecology Hospital, Changchun, Jilin 130042, P.R. China
| | - Yang Cao
- Department of Gynecology and Obstertrics, Changchun Obstetrics‑Gynecology Hospital, Changchun, Jilin 130042, P.R. China
| | - Yunxiao Jia
- Department of Gynecology and Obstertrics, Changchun Obstetrics‑Gynecology Hospital, Changchun, Jilin 130042, P.R. China
| | - Chaoxu Li
- Department of Cerebral Surgery, The People's Hospital of Changchun, Changchun, Jilin 130051, P.R. China
| |
Collapse
|
|
15
|
Kariya S, Okano M, Nishizaki K. An association between Helicobacter pylori and upper respiratory tract disease: Fact or fiction? World J Gastroenterol 2014; 20:1470-1484. [PMID: 24587622 PMCID: PMC3925855 DOI: 10.3748/wjg.v20.i6.1470] [Citation(s) in RCA: 24] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 09/16/2013] [Revised: 11/03/2013] [Accepted: 01/06/2014] [Indexed: 02/06/2023] Open
Abstract
Helicobacter pylori (H. pylori) is a major cause of chronic gastritis and gastric ulcers and considerable evidence supports the notion that infection with this bacterium is also associated with gastric malignancy in addition to various other conditions including pulmonary, vascular and autoimmune disorders. Gastric juice infected with H. pylori might play an important role in upper respiratory tract infection. Although direct and/or indirect mechanisms might be involved in the association between H. pylori and upper respiratory tract diseases, the etiological role of H. pylori in upper respiratory tract disorders has not yet been fully elucidated. Although various studies over the past two decades have suggested a relationship between H. pylori and upper respiratory tract diseases, the findings are inconsistent. The present overview describes the outcomes of recent investigations into the impact of H. pylori on upper respiratory tract and adjacent lesions.
Collapse
|