Comprehensive analysis of cell-extracellular matrix protein Ras suppressor-1 in function and prognosis of gastrointestinal cancers

Table 1 Correlation of Ras suppressor 1 expression and prognosis in gastric cancer with various clinicopathological factors

	OS (631)			FP (522)			PPS (384)
	n	HR	P value	n	HR	P value	n	HR	P value
Sex
Female	187	1.88 (1.22-2.89)	c	179	1.65 (1.09-2.51)	a	127	1.99 (1.21-3.25)	b
Male	349	1.36 (0.94-1.97)	0.098	341	1.4 (0.98-2.01)	0.064	256	1.52 (1.09-2.14)	a
Stage
I	62	2.84 (0.63-12.88)	0.16	60	2.7 (0.6-12.26)	0.18	31	2.79 (0.53-14.68)	0.21
II	135	1.47 (0.79-2.75)	0.22	131	0.73 (0.4-1.32)	0.29	105	2.01 (1.04-3.9)	a
III	197	1.27 (0.87-1.87)	0.22	186	1.51 (1.02-2.22)	a	142	1.2 (0.77-1.86)	0.42
IV	140	1.53 (0.95-2.46)	0.078	141	1.17 (0.74-1.84)	0.51	104	1.79 (1.13-2.83)	a
T
T2	241	1.64 (0.98-2.73)	0.055	239	1.42 (0.88-2.29)	0.15	196	1.57 (0.9-2.72)	0.11
T3	204	1.3 (0.92-1.83)	0.14	204	1.36 (0.95-1.94)	0.091	150	1.36 (0.93-1.99)	0.12
T4	38	3.33 (1.12-9.96)	a	39	2.12 (0.85-5.3)	0.1	29	4.68 (1.34-16.32)	b
N
N0	74	2.77 (1.19-6.43)	a	72	2.69 (1.16-6.23)	a	41	10.98 (2.7-44.62)	c
N1-3	422	1.54 (1.18-2.01)	b	423	1.35 (1.04-1.74)	a	337	1.64 (1.22-2.19)	c
M
M0	444	1.72 (1.2-2.47)	b	443	1.63 (1.16-2.31)	b	342	1.77 (1.31-2.4)	c
M1	56	1.84 (1.02-3.29)	a	56	1.44 (0.79-2.6)	0.23	56	2.09 (0.98-4.45)	0.052
HER2 status
Negative	429	1.46 (1.12-1.91)	b	429	1.55 (1.06-2.26)	a	283	1.79 (1.28-2.5)	c
Positive	202	1.41 (0.95-2.09)	0.086	166	1.46 (0.9-2.38)	0.13	101	1.46 (0.89-2.4)	0.14
Treatment
Surgery alone	380	1.44 (1.07-1.93)	a	375	1.36 (0.97-1.9)	0.071	277	1.82 (1.31-2.52)	c
5-FU-based adjuvant	34	1.77 (0.69-4.52)	0.23	34	1.8 (0.77-4.25)	0.17	21	0.39 (0.13-1.18)	0.085
Other adjuvant	76	2.65 (1.09-6.4)	a	80	3.46 (1.03-11.67)	a	74	2.5 (0.98-6.41)	a

^aP < 0.05.

^bP < 0.01.

^cP < 0.001.

5-FU: 5-fluorouracil; FS: First progression; HR: Hazard ratio; OS: Overall survival; PPS: Post-progression survival.

Table 2 Correlation between Ras suppressor 1 level and immune cell biomarkers in gastrointestinal cancers

Immune cell	Biomarker	COAD				STAD				GBM
		None		Purity		None		Purity		None		Purity
		Cor	P value	Cor	P value	Cor	P value	Cor	P value	Cor	P value	Cor	P value
CD8+ T cell	CD8A	0.052	2.64E-01	0.008	8.73E-01	0.306	c	0.305	c	-0.056	4.90E-01	-0.026	7.62E-01
	CD8B	-0.043	3.60E-01	-0.076	1.25E-01	0.158	b	0.151	b	-0.157	5.30E-02	-0.124	1.50E-01
T cell	CD3D	0.034	4.72E-01	-0.009	8.63E-01	0.23	c	0.228	c	-0.140	8.37E-02	-0.114	1.86E-01
	CD3E	0.123	b	0.099	a	0.248	c	0.245	c	-0.042	6.06E-01	0.007	9.38E-01
	CD2	0.176	c	0.168	c	0.304	c	0.307	c	-0.074	3.61E-01	-0.034	6.96E-01
B cell	CD19	0.116	a	0.099	a	0.183	c	0.161	b	0.019	8.14E-01	-0.047	5.86E-01
	CD79A	0.197	c	0.18	c	0.18	c	0.155	b	0.093	2.55E-01	0.133	1.21E-01
Monocyte	CD86	0.255	c	0.258	c	0.361	c	0.375	c	0.024	7.72E-01	0.104	2.28E-01
	CD115 (CSF1R)	0.189	c	0.176	c	0.439	c	0.44	c	0.200	a	0.302	c
TAM	CCL2	0.352	c	0.337	c	0.348	c	0.355	c	0.034	6.74E-01	0.083	3.35E-01
	CD68	0.096	a	0.094	5.76E-02	0.17	c	0.169	c	0.169	a	0.275	b
	IL10	0.21	c	0.204	c	0.384	c	0.406	c	-0.043	5.98E-01	0.036	6.78E-01
M1 Macrophage	INOS (NOS2)	-0.196	c	-0.196	c	0.035	4.75E-01	0.061	2.36E-01	0.049	5.50E-01	0.082	3.39E-01
	IRF5	0.216	c	0.219	c	0.108	a	0.093	7.03E-02	0.040	6.26E-01	0.094	2.74E-01
	COX2 (PTGS2)	0.084	7.40E-02	0.039	4.31E-01	0.186	c	0.183	c	0.198	a	0.254	b
M2 Macrophage	CD163	0.187	c	0.168	c	0.395	c	0.397	c	0.155	5.51E-02	0.213	a
	VSIG4	0.18	c	0.158	b	0.401	c	0.421	c	0.018	8.25E-01	0.101	2.39E-01
	MS4A4A	0.221	c	0.204	c	0.421	c	0.431	c	0.060	4.58E-01	0.166	5.21E-02
Neutrophils	CD66B (CEACAM8)	-0.024	6.15E-01	-0.013	7.98E-01	0.029	5.57E-01	0.034	5.08E-01	0.033	6.87E-01	0.044	6.11E-01
	CD11B (ITGAM)	0.136	b	0.13	b	0.392	c	0.392	c	0.233	b	0.319	c
	CCR7	0.17	c	0.16	b	0.322	c	0.325	c	0.226	b	0.254	b
Natural killer cell	KIR2DL1	-0.024	6.08E-01	-0.057	2.51E-01	0.143	b	0.145	b	-0.025	7.62E-01	-0.027	7.52E-01
	KIR2DL3	-0.076	1.06E-01	-0.091	6.78E-02	0.094	5.45E-02	0.083	1.07E-01	-0.022	7.88E-01	-0.052	5.48E-01
	KIR2DL4	-0.072	1.23E-01	-0.11	a	0.005	9.25E-01	0.003	9.61E-01	-0.009	9.11E-01	0.009	9.13E-01
	KIR3DL1	-0.049	2.92E-01	-0.073	1.40E-01	0.142	b	0.127	a	0.042	6.06E-01	0.018	8.34E-01
	KIR3DL2	-0.01	8.31E-01	-0.063	2.04E-01	0.146	b	0.147	b	-0.086	2.93E-01	-0.060	4.86E-01
	KIR3DL3	-0.086	6.63E-02	-0.093	6.04E-02	-0.078	1.12E-01	-0.064	2.16E-01	-0.118	1.48E-01	-0.107	2.12E-01
	KIR2DS4	-0.015	7.48E-01	-0.026	5.99E-01	0.051	3.03E-01	0.044	3.95E-01	-0.001	9.88E-01	0.003	9.72E-01
Dendritic cell	HLA-DPB1	0.051	2.73E-01	0.024	6.27E-01	0.287	c	0.291	c	0.032	6.91E-01	0.108	2.08E-01
	HLA-DQB1	0.056	2.29E-01	0.037	4.55E-01	0.152	b	0.153	b	0.152	6.11E-02	0.234	b
	HLA-DRA	0.125	b	0.103	a	0.268	c	0.27	c	-0.049	5.51E-01	0.014	8.73E-01
	HLA-DPA1	0.16	c	0.142	b	0.255	c	0.256	c	0.032	6.91E-01	0.108	2.08E-01
	BDCA-1 (CD1C)	0.29	c	0.291	c	0.303	c	0.302	c	-0.030	7.10E-01	0.025	7.70E-01
	BDCA-4 (NRP1)	0.348	c	0.37	c	0.523	c	0.52	c	0.376	c	0.462	c
	CD11c (ITGAX)	0.156	c	0.15	b	0.341	c	0.348	c	0.081	3.19E-01	0.099	2.49E-01
Th1	T-bet (TBX21)	0.069	1.39E-01	0.06	2.27E-01	0.266	c	0.264	c	0.237	b	0.258	b
	STAT4	0.159	c	0.148	b	0.301	c	0.294	c	-0.091	2.63E-01	-0.043	6.18E-01
	STAT1	0.197	c	0.182	c	0.189	c	0.177	c	0.078	3.38E-01	0.075	3.84E-01
	IFN-γ (IFNG)	0.023	6.28E-01	0.009	8.53E-01	0.098	a	0.091	7.73E-02	0.120	1.40E-01	0.130	1.30E-01
	TNF-α (TNF)	0.118	a	0.098	a	0.104	a	0.071	1.67E-01	0.022	7.83E-01	0.026	7.67E-01
Th2	GATA3	0.176	c	0.183	c	0.305	c	0.304	c	0.125	1.24E-01	0.143	9.67E-02
	STAT6	0.006	8.92E-01	0.01	8.44E-01	0.224	c	0.219	c	0.289	c	0.353	c
	STAT5A	0.155	c	0.172	c	0.431	c	0.426	c	0.071	3.81E-01	0.104	2.24E-01
	IL13	0.124	b	0.099	a	0.151	b	0.153	b	0.021	7.93E-01	0.029	7.39E-01
Tfh	BCL6	0.145	b	0.143	b	0.388	c	0.367	c	0.238	b	0.232	b
	IL21	0.074	1.13E-01	0.06	2.31E-01	0.133	b	0.12	a	0.023	7.73E-01	-0.026	7.67E-01
Th17	STAT3	0.318	c	0.327	c	0.426	c	0.411	c	0.404	c	0.394	c
	IL17A	0.055	2.42E-01	0.057	2.51E-01	-0.124	a	-0.133	b	-0.077	3.42E-01	-0.082	3.42E-01
Treg	FOXP3	0.218	c	0.214	c	0.224	c	0.223	c	0.065	4.24E-01	0.073	3.98E-01
	CCR8	0.317	c	0.327	c	0.341	c	0.336	c	0.052	5.26E-01	0.082	3.38E-01
	STAT5B	0.401	c	0.413	c	0.499	c	0.491	c	0.218	b	0.179	a
	TGFβ (TGFB1)	0.114	a	0.106	3.28E-02	0.418	c	0.406	c	0.325	c	0.387	c
T cell (exhausted)	PD1 (PDCD1)	-0.016	7.28E-01	-0.05	3.16E-01	0.205	c	0.202	c	0.085	2.96E-01	0.132	1.23E-01
	CTLA4	0.1	a	0.092	6.43E-02	0.17	c	0.164	b	0.030	7.12E-01	0.060	4.89E-01
	LAG3	-0.001	9.91E-01	-0.041	4.04E-01	0.202	c	0.196	c	0.116	1.52E-01	0.126	1.42E-01
	TIM-3 (HAVCR2)	0.223	c	0.22	c	0.38	c	0.391	c	0.046	5.76E-01	0.106	2.16E-01
	GZMB	0.028	5.53E-01	0.007	8.89E-01	0.134	b	0.132	b	-0.009	9.15E-01	0.043	6.20E-01

^aP < 0.05.

^bP < 0.01.

^cP < 0.001.

COAD: Colon adenocarcinoma; STAD: Stomach adenocarcinoma; GBM: Glioblastoma multiforme.