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©2013 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Methodol. Mar 26, 2013; 3(1): 11-18
Published online Mar 26, 2013. doi: 10.5662/wjm.v3.i1.11
Published online Mar 26, 2013. doi: 10.5662/wjm.v3.i1.11
Methods for the extraction and RNA profiling of exosomes
Emily Zeringer, Mu Li, Tim Barta, Jeoffrey Schageman, Ketil Winther Pedersen, Axl Neurauter, Susan Magdaleno, Robert Setterquist, Alexander V Vlassov, Life Technologies, Austin, TX 78744, United States
Author contributions: Zeringer E, Li M, Barta T, Schageman J, Pedersen KW and Neurauter A were involved in design of the study, data acquisition, analysis and interpretation of data; Magdaleno S, Setterquist R and Vlassov AV contributed to the conception and design of the study, and wrote the article.
Correspondence to: Alexander V Vlassov, PhD, Life Technologies, 2130 Woodward street, Austin, TX 78744, United States. sasha.vlassov@lifetech.com
Telephone: +1-512-7213843 Fax: +1-512-6510201
Received: February 27, 2013
Revised: March 8, 2013
Accepted: March 18, 2013
Published online: March 26, 2013
Revised: March 8, 2013
Accepted: March 18, 2013
Published online: March 26, 2013
Core Tip
Core tip: Exosomes are small vesicles (30-150 nm) perceived to be carriers of the unique effector or signaling macromolecules (miRNA, ncRNA, mRNA and protein) between very specific cells within our body. The spectrum of current scientific interests ranges from studying the functions and pathways of exosomes to utilizing them in diagnostics and therapeutics development. Here we describe a complete exosome workflow solution: fast and efficient isolation of exosomes; extraction of their cargo; characterization of exosomal RNA content using quantitative reverse transcription-polymerase chain reaction and other techniques.