Microbiological spectrum and probability of transmission of infection from ocular tissues of enucleated eyes harvested from septicemic donors

Table 1 Microbiological culture analysis in group I and II

Group	Blood culture			Conjunctival culture			Corneal culture			Scleral culture			Aqueous culture			Vitreous culture
	Positive		Negative	Positive		Negative	Positive		Negative	Positive		Negative	Positive		Negative	Positive		Negative
Group I (septicaemic), n = 42	16	38.10%	26	12	28.57%	30	3	7.14%	39	4	9.52%	38	2	4.76%	40	1	2.38%	41
Group II (non-septicaemic), n = 222	12	5.40%	210	37	16.66%	185	20	9.01%	202	24	10.81%	198	5	2.25%	217	3	1.35%	219
P value	0.000			0.058			0.485			0.530			0.308			0.502
Odds ratio	10.6			1.99			0.777			0.868			2.162			1.776
95% confidence interval	4.2-27.63			0.938-4.263			0.141-2.815			0.207-2.739			0.199-13.78			0.033-22.74

Table 2 Microbiological spectrum in ocular tissues of study groups

Isolated species	Conjunctival swab		Corneal		Scleral		Aqueous		Vitreous		Blood
	Group I (n = 42)	Group II (n = 222)	Group I (n = 42)	Group II (n = 222)	Group I (n = 42)	Group II (n = 222)	Group I (n = 42)	Group II (n = 222)	Group I (n = 42)	Group II (n = 222)	Group I (n = 42)	Group II (n = 222)
Klebsiella pneumoniae	1	-	1	-	1	1	-	-	-	-	2	-
Streptococcus viridans	-	2	1	1	-	-	-	-	-	-	2	-
Streptococcus pneumoniae	-	-	-	-	-	1	-	-	-	-	-	-
Staphylococcus aureus	3	8	-	6	1	4	-	-	-	-	-	4
Coagulase-negative Staphylococcus	6	19	1	13	2	17	-	5	-	3	2	-
Enterobacter species	-	2	-	-	-	-	-	-	-	-	4	-
Haemophilus influenza	-	-	-	-	-	-	2	-	1	-	-	-
Propionibacterium acnes	1	-	-	-	-	-	-	-	-	-	-	-
Candida albicans	-	-	-	-	-	-	-	-	-	-	2	2
Escherichia coli	-	3	-	-	-	1	-	-	-	-	2	2
Pseudomonas aeruginosa	-	-	-	-	-	-	-	-	-	-	-	4
Mixed1	1	3	-	-	-	-	-	-	-	-	2	-

¹Mixed isolates consist of: Group I: (1) Coagulase-negative Staphylococcus and Streptococcus viridans; group II: From 3 different eyes of three donors; Staphylococcus aureus and Streptococcus viridans; (2) Coagulase-negative Staphylococcus and Streptococcus viridans, and (3) Coagulase-negative Staphylococcus and Enterobacter species.

Table 3 Microbiological analysis of ocular tissues harvested from blood culture positive septicemic group and blood culture negative non-septicemic donors

Group	Conjunctival swab			Corneal tissue			Scleral tissue			Aqueous tap			Vitreous tap
	+	-	P value	+	-	P value	+	-	P value	+	-	P value	+	-	P value
Septicemic (n = 16)	3	13	0.564	2	14	0.477	1	15	0.450	2	14	0.080	1	15	0.256
Non-septicemic (n = 210)	37	173		20	190		24	186		5	205		3	207
Odds ratio	1.079			1.355			0.518			5.768			4.543
95% confidence interval	0.188-4.207			0.140-6.603			0.012-3.672			0.506-39.39			0.082-60.7

+: Positive culture, -: Negative culture.

Table 4 Correlation of death to preservation time with microbial growth in study groups

Group	Culture results	Death to preservation time		P value
		≤ 12 hours	12 hours
Septicemic donors (n = 42)	Positive	0	3	0.0104
	Negative	32	7
Non septicemic donors (n = 222)	Positive	7	13	0.0003
	Negative	153	49