Abnormalities of liver sinusoidal endothelial cells in primary biliary cholangitis

Table 1 Demographic characteristics of primary biliary cholangitis patients (n = 30), mean ± SD

Characteristics
Median age	48 (21-67)
Advanced disease stage (III-IV)	40%
AMA positive	100%
ANA positive	47%
IgM (> 170 mg/dL)	86%
IgM (mg/dL)	363 ± 198
ALP at diagnosis (U/L)	263 ± 171
Total bilirubin (mg/dL)	1.06 ± 1.38
Albumin (g/dL)	4.22 ± 0.51
Mayo risk score	4.31 ± 1.18

AMA: Anti-mitochondrial antibodies; ANA: Anti-nuclear antibodies; IgM: Immunoglobulin M; ALP: Alkaline phosphatase.

Table 2 Primers used in quantitative polymerase chain reaction

Item
Gene name ENDRB	RefSeq ID: NM_000115
Right primer sequence	CCACGGAGGCTTTCTGTATG; Tm: 60.647
Left primer sequence	CACATCGTCATTGACATCCC; Tm: 59.769
cDNA amplicon size	109
Gene name ENDRA	RefSeq ID: NM_001957
Right primer sequence	ATTGTGATCAAAAGGCCAGC; Tm: 60.081
Left primer sequence	GATAGCCAGTCTTGCCCTTG; Tm: 59.836
cDNA amplicon size	100
Gene name ET1	RefSeq ID: NM_001955
Right primer sequence	GACTGGGAGTGGGTTTCTCC; Tm: 60.891
Left primer sequence	TCTCTGCTGTTTGTGGCTTG; Tm: 60.175
cDNA amplicon size	103
Gene name ET2	RefSeq ID: NM_001956
Right primer sequence	TTCCTCCACCTGGAATGTGT; Tm: 60.363
Left primer sequence	AGAGCTTCTCCAAAGGCTGA; Tm: 59.308
cDNA amplicon size	108
Gene name ET3	RefSeq ID: NM_000114
Right primer sequence	GGACAGTCCATAGGGCACC; Tm: 60.342
Left primer sequence	GCACGTGCTTCACCTACAAG; Tm: 59.515
cDNA amplicon size	101
Cyclophilin A	ATGGTCAACCCCACCGTGT; TTCTGCTGTCTTTGGAACTTTGTC

ENDRB: Endothelin receptor B; RefSeq ID: Reference sequence identifier; ENDRA: Endothelin receptor A; ET: Endothelin.