Differences of immunohistochemical profiling of MOC-31, caveolin-1, connexin-43, Ki-67 in ameloblastic fibroma, ameloblastic fibrodentinoma, ameloblastic fibro-odontoma, and odontomas

Figure 1 Immunoexpression of caveolin-1 in ameloblastic fibroma, ameloblastic fibrodentinoma, ameloblastic fibro-odontoma, and odontoma. A: Ameloblastic fibroma showing positivity (++) in ameloblastic epithelial islands and odontogenic mesenchymal cells; B: Ameloblastic fibrodentinoma with positivity (++) only in the ameloblastic epithelial component; C: Ameloblastic fibro-odontoma with positivity (++) in the ameloblastic epithelial component, also showing its relation to the formation of mineralized dental tissues (dentin); D: Odontoma with positivity (++) in the epithelial component. Immunohistochemistry, caveolin-1, A-D: × 20.

Figure 2 Immunoexpression of MOC-31 in ameloblastic fibrodentinoma, ameloblastic fibroma, ameloblastic fibro-odontoma, odontoma, and pericoronal cap. A: Ameloblastic fibrodentinoma with positivity (++) in the stellate reticulum; B: Ameloblastic fibroma with positivity (+) in the stellate reticulum; C: Ameloblastic fibro-odontoma showing positivity (++) in stellate reticulum areas and ameloblasts; D: Ameloblastic fibro-odontoma where positivity is observed in epithelial islands continuous with the formation of mineralized structures; E: Odontoma, which was negative for protein immunoexpression; F: Pericoronal cap with positivity (+) in odontogenic epithelial tissues. Immunohistochemistry, MOC-31, A-F: × 20.

Figure 3 Immunoexpression of connexin-43 in ameloblastic fibroma, ameloblastic fibro-odontoma, and odontoma. A: Ameloblastic fibroma with positivity (++) in odontogenic mesenchymal cells; B: Ameloblastic fibro-odontoma with positive staining (++) in ameloblasts and odontogenic mesenchymal cells; C: Odontoma, which was negative for protein immunoexpression. Immunohistochemistry, connexin-43, A: × 9, B and C: × 20.

Figure 4 Immunoexpression of Ki-67 in ameloblastic fibroma, ameloblastic fibro-odontoma, and odontoma. A: Ameloblastic fibroma with nuclear positivity in 5% of cells with staining in ameloblastic cells; B: Ameloblastic fibro-odontoma with immunoexpression in 4.5%, involving both ameloblasts and odontogenic mesenchymal cells; C: Odontoma, which was negative for protein immunoexpression. Immunohistochemistry, Ki-67, A-C: × 20.