Evaluation of urinary extracellular vesicles and microRNAs to diagnose urogenital tuberculosis

Table 1 Oligonucleotides used in this study

Primer set name	Reverse transcriptase reaction primer (5’ to 3’)	Real-time quantitative PCR primer (5’ to 3’)
miRNA-155-5p	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACCCC	Forward: TCGCTTTAATGCTAATCGTGATA
		Reverse: CCAGTGCAGGGTCCGAGGTA
miRNA-26a-5p	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGCCTA	Forward: TCGCTTTCAAGTAATCCAGGA
		Reverse: CCAGTGCAGGGTCCGAGGTA
miRNA-29a-3p	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTAACCG	Forward: TCGCTTAGCACCATCTGAAAT
		Reverse: CCAGTGCAGGGTCCGAGGTA
U6	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAATATGGAACGCTT	Forward: AGAAGATTAGCATGGCCCCTG
		Reverse: CCAGTGCAGGGTCCGAGGTA

PCR: Polymerase chain reaction; MiRNAs: MicroRNA.

Table 2 Demographic characteristics of the study subjects

Characteristics	Healthy control	UTI	UGTB
n	70	69	89
Gender (male), n (%)	32 (45.71)	35 (50.72)	43 (48.31)
Age (year), mean ± SD	40 ± 15	42 ± 14	42 ± 15
Age (groups), n
18-35 years	35	27	36
35-50 years	15	18	19
51-65 years	20	24	34

UTI: Urinary tract infections; UGTB: Urogenital tuberculosis.

Table 3 Culture results of 69 Urinary tract infection samples

Organism	Number
Escherichia coli	40
Other lactose fermenters	5
Non-lactose fermenters	8
Gram positive organisms	17

Table 4 Mycobacterium tuberculosis culture results of 89 urogenital tuberculosis samples

Mycobacterium tuberculosis	Culture results
Culture positive	18
Culture sterile	55
Contamination	01
Only smear	15

Table 5 Receivers operating characteristic curve comparison with cut-off values, sensitivity, specificity and likelihood ratio

Classification	Cut-off	Sensitivity (%)	95%CI	Specificity (%)	95%CI	Likelihood ratio
UGTB vs UTI
miRNA-155-5p	> 2.680	78.95	56.67%-91.49%	85.00	63.96%-94.76%	5.263
miRNA-29a-3p	< 2.945	65.00	43.29%-81.88%	85.00	63.96%-94.76%	4.333
UGTB vs HC
miRNA-155-5p	> 2.320	65.00	43.29%-81.88%	70.00	48.10%-85.45%	2.167
miRNA-26a-5p	> 1.565	70.00	48.10%-85.45%	75.00	53.13%-88.81%	2.800

CI: Confidence interval; UTI: Urinary tract infections; UGTB: Urogenital tuberculosis; HC: Healthy control; MiRNAs: MicroRNA.