Case Control Study
Copyright ©The Author(s) 2023.
World J Exp Med. Dec 20, 2023; 13(5): 102-114
Published online Dec 20, 2023. doi: 10.5493/wjem.v13.i5.102
Table 1 The association of microRNA-125a, tumor necrosis factor-alpha, and interleukin 12 with major clinical symptoms of systemic lupus erythematosus patients
Characteristics
miR-125a
TNF-α
IL-12
Lupus nephritisYes0.199 ± 0.07P = 0.57653.42 ± 14.44P = 0.153122.37 ± 22.36P = 0.104
No0.205 ± 0.0945.60 ± 12.13108.34 ± 29.87
Malar rashYes0.180 ± 0.06P = 0.01754.60 ± 12.44P = 0.360128.45 ± 20.28P = 0.065
No0.216 ± 0.0943.37 ± 11.51102.42 ± 28.94
Hair lossYes0.188 ± 0.06P = 0.02252.63 ± 13.08P = 0.401126.87 ± 20.62P = 0.049
No0.211 ± 0.0944.34 ± 11.98103.19 ± 29.48
SLEDAI≤ 40.202 ± 0.08P = 0.00544.00 ± 14.71P = 0.001102.43 ± 23.38P = 0.000
5-120.280 ± 0.0830.49 ± 12.5969.54 ± 32.43
≥ 120.199 ± 0.0949.11 ± 13.51113.26 ± 28.54
Table 2 List of primers for microRNA-125a and U6 internal control
Primer
Sequence (5'>3')
miR-125aF: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCGT
R: GTCGTATCCAGTGCAGGGTCCGAGGTGCCGAGGATTTCCACCACCTG
U6F: GCTTCGGCAGCACATATACTAAAAT
R: CGCTTCACGAATTTGCGTGTCAT