Circulating levels of growth differentiation factor-15 and its genetic variants -3148C>G (rs4808793) in pediatric cardiac patients

Figure 1 Polymerase chain reaction amplification and digestion products of growth differentiation factor-15 rs4808793 (-3148C>G) single-nucleotide polymorphism using the restriction fragment length polymorphism- polymerase chain reaction technique. A: Lane 1 and 6 referred to 100-bp DNA ladder; lanes 2, 3, 4, and 5 showed undigested amplified polymerase chain reaction product bands at 665 bp; B: Lanes 2, 4, 6, 7, and 8 referred to wild homozygous (CC) genotype with 665 bp bands; lane 3 referred to homozygous variant (GG) genotype with 422 bp, and 243 bp bands; lane 5 referred to heterozygous variant (CG) genotype with 665 bp, 422 bp, and 243 bp bands.

Figure 2 Receiver operator characteristics curve of serum growth differentiation factor-15 levels (pg/mL) among pediatric patients. A: In predicting pediatric patients with congenital heart disease; B: In predicting heart failure among patients with cardiac diseases.