Cytokine gene polymorphisms in idiopathic pulmonary fibrosis

Table 1 Demographic and basic clinical parameters of idiopathic pulmonary fibrosis patients, mean ± SD (range)

Parameters	Value
Men:women ratio	37:19
Mean age (yr)	67.29 ± 11.85
Mean FVC (%)	70.96 ± 20.60 (21-127)
TLCO (%)	39.78 ± 16.63 (13-81)
BALF LY (%)	17.86 ± 18.93 (0-72)
BALF PMN (%)	15.84 ± 17.58 (1-70)
BALF EOS (%)	3.21 ± 6.95 (0-28)

BALF: Bronchoalveolar lavage fluid; FVC: Forced vital capacity; TL_CO: Transfer factor for CO; LY: Lymphocytes; PMN: Polymorphonuclear leukocytes; EOS: Eosinophilic leukocytes.

Table 2 List of investigated cytokine gene polymorphisms

Polymorphism	Genotype
IL-1α (-889)	C/C C/T T/T
IL-1β (-511)	C/C C/T T/T
IL-1β (+3962)	C/C C/T T/T
IL-1R pst 1970	C/C C/T T/T
IL-1 RA mspa 11100	C/C C/T T/T
IL-1 RA (+1902)	A/A A/G G/G
IL-12 (-1188)	A/A A/C C/C
INF-β UTR 5644	A/A A/T T/T
TGF-β1 codon 10	C/C C/T T/T
TGF-β1 codon 25	C/C C/G G/G
TNF-α (-308)	A/A A/G G/G
TNF-α (-238)	A/A A/G G/G
IL-2 (-330)	G/G G/T T/T
IL-2 (+166)	G/G G/T T/T
IL-4 (-1098)	G/G G/T T/T
IL-4 (-590)	C/C C/T T/T
IL-4 (-33)	C/C C/T T/T
IL-6 (-174)	C/C C/G G/G
IL-6 (+565)	A/A A/G G/G
IL-10 (-1082)	A/A A/G G/G
IL-10 (-819)	C/C C/T T/T
IL-10 (-592)	A/A A/C C/C

IL: Interleukin; TNF: Tumor necrosis factor; INF: Interferon; UTR: Untranslated region.

Table 3 IL-4 (-590), IL-4 (-33) and IL-4 haplotype 1 polymorphisms in idiopathic pulmonary fibrosis and healthy controls n (%)

Locus	Genotype	Controls	IPF group	P value
IL-4 (-590)	CC	112 (77.8)	24 (42.9)	< 0.0001
	CT	27 (18.8)	31 (55.4)
	TT	5 (3.5)	1 (1.8)
IL-4 (-33)	CC	112 (77.8)	29 (52.7)	0.0006
	CT	27 (18.8)	25 (45.5)
	TT	5 (3.5)	1 (1.8)
IL-4 haplotype 1	GCC	0 (0.0)	1 (1.8)	< 0.0001
	TCC	112 (77.8)	23 (41.1)
	TCT	0 (0.0)	1 (1.8)
	TTC	0 (0.0)	10 (17.9)
	TTT	32 (22.2)	21 (37.5)

IPF: Idiopathic pulmonary fibrosis; IL: Interleukin.