Copyright
©The Author(s) 2017.
World J Hematol. Feb 6, 2017; 6(1): 17-23
Published online Feb 6, 2017. doi: 10.5315/wjh.v6.i1.17
Published online Feb 6, 2017. doi: 10.5315/wjh.v6.i1.17
Table 1 Primer sequences of genes
| Forward | Reverse | |
| Mouse Flvcr1 | ||
| 5’-CAGTTGATAGTCGGGTAGATCCAA-3' 5'-ACACCGGCTTCTTCAGAGTGA-3’ | ||
| Mouse Abcg2 | ||
| 5’-TCGCAGAAGGAGATGTGTTGAG-3 5CCAGAATAGCATTAAGGCCAGG-3’ | ||
| Mouse HO-1 | ||
| 5’-CAAGGAGGTACACATCCAAGCC-3' 5'-TACAAGGAAGCCATCACCAGCT-3’ | ||
| Mouse 18S | ||
| 5’-GAATTCCCAGTAAGTGGCGGG-3' 5'-GGGCAGGGACTTAATCAACG-3’ | ||
Table 2 Iron parameters of wild type and hemochromatosis gene(-/-) mice after 24 h of feeding heme
| Serum iron (μmol/L) | % Transferrin saturation | ||
| Control | 25.3 ± 4.4 | 32.1 ± 6.5 | |
| WT | Heme-24 h | 26.8 ± 3.1 | 35.9 ± 7.9 |
| Control | 49.8 ± 5.0 | 59.8 ± 6.1 | |
| Hfe(-/-) | Heme-24 h | 50.5 ± 10.6 | 61.0 ± 10.4 |
- Citation: Laftah AH, Simpson RJ, Latunde-Dada GO. Intestinal heme absorption in hemochromatosis gene knock-out mice. World J Hematol 2017; 6(1): 17-23
- URL: https://www.wjgnet.com/2218-6204/full/v6/i1/17.htm
- DOI: https://dx.doi.org/10.5315/wjh.v6.i1.17