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©2014 Baishideng Publishing Group Inc.
World J Biol Chem. Aug 26, 2014; 5(3): 387-397
Published online Aug 26, 2014. doi: 10.4331/wjbc.v5.i3.387
Published online Aug 26, 2014. doi: 10.4331/wjbc.v5.i3.387
Table 1 Taqman probe and primer sequences for quantitative real-time PCR
| Gene | Forward primer (5’-3’) | Reverse primer (5’-3’) | Taqman probe |
| HAMP | TGCCCATGTTCCAGAGGC | CCGCAGCAGAAAATGCAGAT | AAGGAGGCGAGACACCCACTTCCC |
| Human Gapdh | TGAAGGTCGGAGTCAACGG | AGAGTTAAAAGCAGCCCTGGTG | TTTGGTCGTATTGGGCGCCTGG |
| Hepcidin-1 | TGCAGAAGAGAAGGAAGAGAGACA | CACACTGGGAATTGTTACAGCATT | CAACTTCCCCATCTGCATCTTCTGCTGT |
| Hepcidin-2 | GCGATCCCAATGCAGAAGAG | TGTTACAGCACTGACAGCAGAATC | AGGAAGAGAGACATCAACTTCCCCATCTGC |
| Mouse Gapdh | TCACTGGCATGGCCTTCC | GGCGGCACGTCAGATCC | TTCCTACCCCCAATGTGTCCGTCG |
Table 2 Primer sequences for SYBR green quantitative real-time PCR
| Gene | Forward primer (5’-3’) | Reverse primer (5’-3’) |
| Mouse SAA3 | GCCTGGGCTGCTAAAGTCAT | TGCTCCATGTCCCGTGAAC |
| Mouse IL-6 | CTGCAAGAGACTTCCATCCAG | AGTGGTATAGACAGGTCTGTTGG |
| Mouse Gapdh | GTGGAGATTGTTGCCATCAACGA | CCCATTCTCGGCCTTGACTGT |
- Citation: Lu S, Zmijewski E, Gollan J, Harrison-Findik DD. Apoptosis induced by Fas signaling does not alter hepatic hepcidin expression. World J Biol Chem 2014; 5(3): 387-397
- URL: https://www.wjgnet.com/1949-8454/full/v5/i3/387.htm
- DOI: https://dx.doi.org/10.4331/wjbc.v5.i3.387