High tolerance to mutations in a Chlamydia trachomatis peptide deformylase loop

Table 1 Effects of mutations in the DGELV loop region on catalytic properties

	Wild-type	D68R	E70R	D68R/E70R	Δ68-72
Km
mM (mean ± SD)	6.8 ± 0.7	7.4 ± 1.2	6.1 ± 1.7	6.7 ± 0.4	18.6 ± 2.9
% control	100	109	91	99	275
kcat
s-1 (mean ± SD)	37.9 ± 2.6	93.1 ± 8.4b	27.9 ± 4.3a	113.1 ± 10.9b	197.0 ± 29.0b
% control	100	246	74	299	520
kcat/Km
M-1s-1 (mean ± SD)	5605 ± 357	12764 ± 970b	4652 ± 677	16781 ± 678b	10605 ± 301b
% control	100	228	83	299	189

^aP < 0.05,

^bP < 0.01 (2-tailed Student t test for comparison with the wild-type chlamydial peptide deformylase).