Fasciculation and elongation zeta proteins 1 and 2: From structural flexibility to functional diversity

Table 1 Identities and similarities between human proteins FEZ1, FEZ2 and UNC-76 from C. elegans

	UNC-76 identity (%)	UNC-76 similarity (%)	FEZ1 identity (%)	FEZ1 similarity (%)
FEZ1	35	46	-	-
FEZ2	34	45	49	56

Alignment of 376 and 385 amino acid UNC-76, 251 amino acid FEZ2, and 392 amino acid FEZ1. It is noteworthy that the N-terminal region shows to be more divergent, but yet with substantial similarity. The C-terminal region presented three particular regions with more similarity (amino acids 179-197, 251-307, and 354-381 – numbered according to the C. elegans protein)[1]. FEZ: Fasciculation and elongation zeta/zygin; UNC: Uncoordinated.

Table 2 FEZ1 interaction partners¹ involved in autophagy

Gene	Protein	FEZ1 interaction reference	Autophagy reference
RAB3GAP2	Rab3 GTPase-activating protein non-catalytic subunit	[13]	[48]
RAB3GAP1	Rab3 GTPase-activating protein catalytic subunit	[13]	[48]
FEZ1	Fasciculation and elongation protein zeta-1	[13]	[43]
FEZ2	Fasciculation and elongation protein zeta-2	[13]	[43]
TBC1D25	TBC1 domain family member 25	[13]	[49]
HAP1	Huntingtin-associated protein 1	[57]	[50]
HTT	Huntingtin	[57]	[51]
SCOCO	Short coiled-coil protein	[13]	[43]
TLK2	Serine/threonine-protein kinase tousled-like 2	[13]	[43]
NBR1	Next to BRCA1 gene 1 protein	[6]	[52]
PTPRS	PTPRS protein	[55]	[53]
TTR	Transthyretin	[55]	[54]
ULK1	UNC-51 like kinase 1	[43]	[56]

¹Interactions described to Homo sapiens sequence. Rab: Ras superfamily of small GTP-binding proteins; TBC: Tre-2/Bub2/Cdc16; BRCA: Breast cancer gene; PTPRS: Protein tyrosine phosphatase, receptor type S; UNC: Uncoordinated, serine/threonine protein kinase.

Table 3 FEZ interaction protein partners related to nuclear function and transcription

Protein	Description	Reference
DRAP1 (NC2α)	DR1 associated protein 1 (negative co-factor 2 alpha)	[13]
BAF60a (SMARCD1)	Component of SWI/SNF chromatin remodeling complex	[13]
SAP30L	Sin3A-associated protein, 30 kDa-like	[2,13]
BRD1	Bromodomain containing protein 1	[13]
TLK2	Tousled like kinase 2	[13]
ZNF251	Zinc finger protein 251	[13]
RARA	Retinoic acid receptor alpha	[2,61]
MED7	Mediator complex subunit 7	[2]
MLF1IP	MLF1 interacting protein	[2]
SAP30	Sin3A-associated protein, 30kDa	[2]
SFRS8	Splicing factor, arginine/serine-rich 8	[2]

DR1: Down-regulator of transcription 1; SMARCD1: SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator of Chromatin, Subfamily D, Member 1; BAF60a: Brg1/Brm associated factor, 60‐kDa, subunit A (Brm: brahma, Brg1: brahma-related gene 1); SWI/SNF: Switching defective/sucrose nonfermenting; MLF1: Myeloid leukemia factor 1.