Potential role of microRNA-503 in Icariin-mediated prevention of high glucose-induced endoplasmic reticulum stress

Table 1 Sequences of miR-503 mimic/inhibitor

miRNA	Sequence (5’-3’)
miR-503 mimic	TAGCAGCGGGAACAGTACTGCAG
miR-503 mimic NC	GACAGAGACACGAGCGGCTGTAT
miR-503 inhibitor	CTGCAGTACTGTTCCCGCTGCTA
miR-503 inhibitor NC	TAGTAACCGTCTTTCCCCTGGGC

miRNA/miR: microRNA.

Table 2 Reverse transcription-quantitative polymerase chain reaction primers

Primer	Sequence (5’-3’)
miR-151-3p-F	ACACTCCAGCTGGGCUAGACUGAGGCUCC
miR-151-3p-R	CTCAACTGGTGTCGTGGA
miR-19b-3p-F	ACACTCCAGCTGGGUGUGCAAAUCCAUGCAA
miR-19b-3p-R	CTCAACTGGTGTCGTGGA
miR-106b-5p-F	ACACTCCAGCTGGGUAAAGUGCUGACAGU
miR-106b-5p-R	CTCAACTGGTGTCGTGGA
miR-30e-5p-F	ACACTCCAGCTGGGUGUAAACAUCCUUGAC
miR-30e-5p-R	CTCAACTGGTGTCGTGGA
miR-122-5p-F	ACACTCCAGCTGGGUGGAGUGUGACAAUGG
miR-122-5p-R	CTCAACTGGTGTCGTGGA
miR-29a-3p-F	ACACTCCAGCTGGGUAGCACCAUCUGAAAU
miR-29a-3p-R	CTCAACTGGTGTCGTGGA
miR-29c-3p-F	ACACTCCAGCTGGGUAGCACCAUUUGAAAU
miR-29c-3p-R	CTCAACTGGTGTCGTGGA
miR-497-5p-F	ACACTCCAGCTGGGCAGCAGCACACUGUGG
miR-497-5p-R	CTCAACTGGTGTCGTGGA
miR-101a-3p-F	ACACTCCAGCTGGGUACAGUACUGUGAUA
miR-101a-3p-R	CTCAACTGGTGTCGTGGA
miR-741-3p-F	ACACTCCAGCTGGGAAAGAUGCCACGCUAU
miR-741-3p-R	CTCAACTGGTGTCGTGGA
miR-503-F	ACACTCCAGCTGGGTAGCAGCGGGAACAGTA
miR-503-R	CTCAACTGGTGTCGTGGA
miR-671-F	ACACTCCAGCTGGGUCCGGUUCUCAGGGC
miR-671-R	CTCAACTGGTGTCGTGGA
U6-F	CTCGCTTCGGCAGCACA
U6-R	AACGCTTCACGAATTTGCGT
SIRT4-F	TCCTGGGAGTGGACAGAATGA
SIRT4-R	CTGTGGATCCATGGGAACGC
Caspase 12-F	TGCCAATTCCGACAAACAGC
Caspase 12-R	CTGGATTCCCTGAGGAACTGT
GRP78-F	AACCCAGATGAGGCTGTAGCA
GRP78-R	ACATCAAGCAGAACCAGGTCAC
CHOP-F	CCAGCAGAGGTCACAAGCAC
CHOP-R	CGCACTGACCACTCTGTTTC
β-actin-F	CACCCGCGAGTACAACCTTC
β-actin-R	CCCATACCCACCATCACACC

F: Forward; R: Reverse; miRNA/miR: microRNA; SIRT4: Sirtuin 4; CHOP: C/EBP-homologous protein; GRP78: Glucose regulated protein 78.

Table 3 Detection of 12 differentially expressed miRNAs between diabetes mellitus rats and normal rats by miRNA sequencing

miRNA_id	Log2 FC	P value
rno-miR-151-3p	> 1.0	< 0.001
rno-miR-19b-3p	< -1.0	< 0.001
rno-miR-106b-5p	< -1.0	< 0.001
rno-miR-30e-5p	< -1.0	< 0.001
rno-miR-122-5p	> 1.0	< 0.001
rno-miR-29a-3p	< -1.0	< 0.001
rno-miR-29c-3p	< -1.0	< 0.001
rno-miR-497-5p	< -1.0	< 0.001
rno-miR-101a-3p	< -1.0	< 0.001
rno-miR-741-3p	> 1.0	< 0.001
rno-miR-503	< -1.0	< 0.001
rno-miR-671	> 1.0	< 0.001

miRNA/miR: microRNA; FC: Fold-change.

Table 4 Identification of mRNAs that may bind to miR-503 by sequencing results combined with the miRDB database

No.	Gene_id
1	SLC12A1
2	SIRT4
3	MLYCD
4	FBXL22
5	CCND1
6	MAP2K1
7	PCP4
8	RPS6KA3
9	EMC6
10	PPFIA3
11	TTC17
12	RAB11FIP1
13	GALNT2
14	NCS1
15	APLN
16	CEP85L
17	CLSTN1
18	KIF1C
19	PIGQ
20	BTRC
21	LOC691995
22	EGLN3
23	NABP1