Tongxinluo promotes endothelium-dependent arteriogenesis to attenuate diabetic peripheral arterial disease

Table 1 Primary and secondary antibodies

Antibodies	Company	Dilution
CD31	abcam, ab281583	1:300 dilution
α-SMA	abcam, ab124964	1:300 dilution
Caspase-1	Servicebio, GB11383	1:500 dilution
Cleaved-caspase-1	Immunoway, YC002	1:1000 dilution
NLRP3	Immunoway, YT5382	1:1000 dilution
GSDMD	Proteintech, 66387-1-l g	1:5000 dilution
ICAM-1	abcam, ab222736	1:1000 dilution
VCAM-1	abcam, ab134047	1:1000 dilution
eNOS	abcam, ab5589	1:1000 dilution
VEGF	abcam, ab52917	1:5000 dilution
CD45	Proteintech, 60287-1-l g	1:200 dilution
TNF-α	abcam, ab183218	1:1000 dilution
VE-cadherin	Proteintech, 27956-1-AP	1:1000 dilution
β-catenin	Proteintech, 51067-2-AP	1:5000 dilution
F4/80	Servicebio, GB113373	1:500 dilution
iNOS	abcam, ab15323	1:250 dilution
Arg-1	Proteintech, 66129-1-l g	1:1000 dilution
Jagged-1	Immunoway, YT5401	1:1000 dilution
Notch-1	Servicebio, GB111690	1:500 dilution
ephrin-B2	Immunoway, YT1586	1:1000 dilution
MMP9	Servicebio, GB11132	1:1000 dilution
MMP2	Servicebio, GB11130	1:1000 dilution
GAPDH	Servicebio, G3206-1OD	1:2000 dilution
α-tubulin	Servicebio, GB15139	1:500 dilution
Goat anti-rabbit IgG	abcam, ab216777	1:5000 dilution
Goat anti-mouse IgG H&L	Abcam, ab216772	1:5000 dilution
Alexa Flour® 488 goat anti-rabbit IgG	Servicebio, GB25303	1:250 dilution
Cy3-conjugated goat	Servicebio, GB25303	1:250 dilution
anti-mouse IgG

α-SMA: α-smooth muscle actin; NLRP3: Nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; ICAM-1: Intercellular cell adhesion molecule-1; VCAM-1: vascular cell adhesion molecule-1; eNOS: Endothelial nitric oxide synthase; VEGF: Vascular endothelial growth factor; TNF-α: Tumor necrosis factor-α; iNOS: Inducible nitric oxide synthase; Arg-1: Arginase-1; MMP9: Matrix metalloproteinase9; MMP2: Matrix metalloproteinase 2.

Table 2 Primer sequences

Gene	Species	Forward sequence (5’-3’)	Reverse sequence (5’-3’)
PCNA	Mouse	GTCGGGTGAATTTGCACGTA	CTCTATGGTTACCGCCTCCTC
GAPDH	Mouse	CCTCGTCCCGTAGACAAAATG	TGAGGTCAATGAAGGGGTCGT
Jagged-1	Human	TGTGGCTTGGATCTGTTGCTTGG	ACGTTGTTGGTGGTGTTGTCCTC
Notch-1	Human	GTGCCCTGGGCTTCTCTG	GGCACGATTTCCCTGACCA
ephrin-B2	Human	CGTCCAGAACTAGAAGCTGGT	CCGTCTGTGCTAGAACCTGG
VEGF	Human	ACAAATGTGAATGCAGACCAAA	ACCAACGTACACGCTCCAG
eNOS	Human	GCCGGAACAGCACAAGAGTTA	CCCTGCACTGTCTGTGTTACT
VE-Cadherin	Human	CAGCCCAAAGTGTGTGAGAA	CGGTCAAACTGCCCATACTT
β-catenin	Human	AGCTTCCAGACACGCTATCAT	CGGTACAACGAGCTGTTTCTAC
GAPDH	Human	GGAAGCTTGTCATCAATGGAAATC	TGATGACCCTTTTGGCTCCC

VEGF: Vascular endothelial growth factor; eNOS: Endothelial nitric oxide synthase.