Role of CCM3 in lead-induced neurological damage in diabetes

Figure 1 The blood glucose levels of mice in each group at four time points. WT: Wild-type.

Figure 2 The blood lead levels of mice in each group. ^aP < 0.05. WT: Wild-type.

Figure 3 Results of water maze experiment on mice in each group. A: Representative diagram of swimming trajectory of mice on day 5; B: Escape latency of mice on day 5. ^aP < 0.05 vs wild-type control group. WT: Wild-type.

Figure 4 Fluorescence co-localization results of CD31 and glial fibrillary acidic protein in the hippocampus of mice in each group. A: Expression of CD31 and glial fibrillary acidic protein (GFAP) in the hippocampus of mice in each group, B: Average fluorescence intensity of CD31, C: Average fluorescence intensity of GFAP. ^aP < 0.05 vs wild-type control group, ^cP < 0.05 vs wild-type diabetes group. WT: Wild-type.

Figure 5 Differential protein volcano and AKT protein abundance difference. A: Differential protein volcano between wild-type (WT) control group and CCM3^+/- diabetes group; B: Differential protein volcano between CCM3^+/- control group and CCM3^+/- diabetes group; C: Differential protein volcano between WT diabetes lead exposed group and CCM3^+/- diabetes group; D: AKT protein abundance difference between WT control group and CCM3^+/- diabetes group; E: AKT protein abundance difference between CCM3^+/- control group and CCM3^+/- diabetes group; F: AKT protein abundance difference between WT diabetes lead exposed group and CCM3^+/- diabetes group.

Figure 6 Western blot results of hippocampal tissue of mice in each group. A: Expression of CCM3 and GPX4 in the hippocampus of mice in each group; B: Expression of PI3K and AKT in the hippocampus of mice in each group. ^aP < 0.05. WT: Wild-type.

Figure 7 Glutathione levels in the hippocampus of mice. ^aP < 0.05. WT: Wild-type.

Figure 8  Research population screening process.

Figure 9 Heat map of correlation between urinary lead levels, blood glucose levels and rs6784267 locus with four neurotransmitter metabolites. ^aP < 0.05. VMA: Vanillylmandelic acid; HVA: Homovanillic acid; DOPAC: 3,4-dihydroxyphenylacetic acid; 5-HIAA: 5-hydroxyindole acetic acid.