Endocytoscopic visualization of squamous cell islands within Barrett’s epithelium

Figure 1 White light endoscopy, narrow-band imaging and endocytoscopy examination of long segment Barrett’s esophagus. A: Long segment Barrett’s esophagus under white light endoscopy (WLE); B: Narrow-band imaging with low magnification clearly visualized small squamous cell islands within regular columnar Barrett's epithelium, which are also identified by WLE with difficulty; C: Endocytoscopy (ECS) examination after crystal violet and methylene blue (CM) double staining; D: ECS examination under higher magnification (× 480) shows the glandular orifices of regular Barrett’s epithelium.

Figure 2 Endocytoscopy examination of histologically confirmed squamous cell islands within Barrett’s esophagus. A: Endocytoscopy (ECS) examination shows squamous cell islands, within regular glandular structures of Barrett’s esophagus. According to ECS examination, squamous papillary structure is classified as ECA2, (round-shaped cells with different-sized small nuclei, suggestive of inflammatory changes); B: Histological examination (hematoxylin and eosin stain magnification) of biopsies from the same area as in Figure (A) confirmed the presence of a squamous papillary structure surrounded by Barrett’s glandular epithelium.