Liao C, Zhao MJ, Zhao J, Jia D, Song H, Li ZP. Over-expression of LPTS-L in hepatocellular carcinoma cell line SMMC-7721 induces crisis. World J Gastroenterol 2002; 8(6): 1050-1052 [PMID: 12439923 DOI: 10.3748/wjg.v8.i6.1050]
Corresponding Author of This Article
Professor Mu-Jun Zhao, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. mjzhao@sunm.shcnc.ac.cn
Article-Type of This Article
Liver Cancer
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World J Gastroenterol. Dec 15, 2002; 8(6): 1050-1052 Published online Dec 15, 2002. doi: 10.3748/wjg.v8.i6.1050
Over-expression of LPTS-L in hepatocellular carcinoma cell line SMMC-7721 induces crisis
Cheng Liao, Mu-Jun Zhao, Jing Zhao, Di Jia, Hai Song, Zai-Ping Li
Cheng Liao, Mu-Jun Zhao, Jing Zhao, Di Jia, Hai Song, Zai-Ping Li, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Author contributions: All authors contributed equally to the work.
Supported by a grant from National High Technology “863” Program of China No. 2001AA221021, a grant from Special Funds for Major State Basic Research “973” of China No. 001CB510205 and a grant from the National Natural Sciences Foundation of China No. 30170524.
Correspondence to: Professor Mu-Jun Zhao, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. mjzhao@sunm.shcnc.ac.cn
Received: May 16, 2002 Revised: May 30, 2002 Accepted: June 3, 2002 Published online: December 15, 2002
Abstract
AIM: To evaluate the function of the longer transcripts LPTS-L in hepatocellular carcinoma cell line SMMC-7721.
METHODS: SMMC-7721 cells were transfected with LPTS-L expression construct and stably transfected cells were selected by G418. Multiple single clones formed and were checked for their phenotype. In the study of the effect on telomerase activity of LPTS-L in vitro, GST-LPTS-L fusion protein was expressed in E.coli and purified by glutathione-agarose column. Telomeric repeat amplification protocol (TRAP) assays were performed to study the influence of telomerase activity in SMMC-7721 cells.
RESULTS: Over-expression of LPTS-L induced SMMC-7721 cells into crisis. LPTS-L could inhibit the telomerase activity in SMMC-7721 cells in vitro.
CONCLUSION: LPTS-L is a potent telomerase inhibitor. Over-expression of LPTS-L can induce hepatoma cells into crisis due to the reduction of telomerase activity.
